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The map label for this gene is pilD [H]

Identifier: 118579418

GI number: 118579418

Start: 1023994

End: 1024779

Strand: Direct

Name: pilD [H]

Synonym: Ppro_0982

Alternate gene names: 118579418

Gene position: 1023994-1024779 (Clockwise)

Preceding gene: 118579417

Following gene: 118579419

Centisome position: 25.55

GC content: 58.14

Gene sequence:

>786_bases
ATGACGCCGCAGCTCTTGTTTTCGGTCTTTTCCTTTATTCTTGGCGCTGTTGTCGGCTCCTTCCTCAATGTCTGCATCTG
CCGCATGCCGCGGGACGAGTCGGTCGTATCGCCCCCCTCCCACTGTCCGGGGTGTGGCTACCGGATTCGTTGGTACGACA
ATATTCCGCTGGTAAGCTACCTGCTGCTGCGCGGCAGGTGCCGGGGCTGCCGGGAAAAGATATCCCCCCGCTACCCTCTG
GTGGAGTTGCTCAACGCCCTTCTGTCTCTGGCGCTTTTTCTCCGCTTCGGGCCGACTCTCGCGTTTGCTGTCCTGTTCCT
CTTCTGTTCCGCGCTGGTTGTCATAACCTTTATCGACCTGGAGCATCAGATTATTCCCGACGAGATATCCCTGCCCGGTA
TCGTGCTCGGTTTCATCTTTTCTTTCTTCCTCAGGGGGCACGGCTGGCTGAACTCGCTTTTGGGCATACTTCTGGGAGGG
GGGAGCCTGCTGTTGGTGGCCTACGGTTACCAGTTGGTGACCGGCAAGGAGGGGATGGGGGGCGGTGATATCAAGCTTTT
GGCCATGATGGGGGCTTTTCTGGGGTGGAAATCCATCCCGTTCATCATCTTCGTCAGTTCGCTGGCCGGCTCGGTGATCG
GCGTTTCCCTGATGCTGATCCAGAAGAAAGATTCGAAGCTGGCCATTCCCTTTGGCCCCTATCTCGCCCTGGGCGCCCTG
CTGTACGTTTTTTACGGCCCCCGTCTGATCAGCTGGTACCTCACCCTGGGTGGTCTCCAGGGGTAA

Upstream 100 bases:

>100_bases
CGCCTGCTTCGCGTTTTCCACCGCCGAACAGGTTGACCTGTTCCTCAGGAATGCCATTGCCACCACGTTTTCCGATACCT
TGGATTGAGACGGGATCCCC

Downstream 100 bases:

>100_bases
GTCCATGCTCAAATGCCGCCCGAGCCTGATCGTATCAATCATGGCCTTCCTGGGGGGGCTTCTGGTATTGACCTGGCTGC
TCTTCAGCCTGCTGGCCTTT

Product: prepilin peptidase

Products: NA

Alternate protein names: Protein pilD; Protein secretion protein XCPA; Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTPQLLFSVFSFILGAVVGSFLNVCICRMPRDESVVSPPSHCPGCGYRIRWYDNIPLVSYLLLRGRCRGCREKISPRYPL
VELLNALLSLALFLRFGPTLAFAVLFLFCSALVVITFIDLEHQIIPDEISLPGIVLGFIFSFFLRGHGWLNSLLGILLGG
GSLLLVAYGYQLVTGKEGMGGGDIKLLAMMGAFLGWKSIPFIIFVSSLAGSVIGVSLMLIQKKDSKLAIPFGPYLALGAL
LYVFYGPRLISWYLTLGGLQG

Sequences:

>Translated_261_residues
MTPQLLFSVFSFILGAVVGSFLNVCICRMPRDESVVSPPSHCPGCGYRIRWYDNIPLVSYLLLRGRCRGCREKISPRYPL
VELLNALLSLALFLRFGPTLAFAVLFLFCSALVVITFIDLEHQIIPDEISLPGIVLGFIFSFFLRGHGWLNSLLGILLGG
GSLLLVAYGYQLVTGKEGMGGGDIKLLAMMGAFLGWKSIPFIIFVSSLAGSVIGVSLMLIQKKDSKLAIPFGPYLALGAL
LYVFYGPRLISWYLTLGGLQG
>Mature_260_residues
TPQLLFSVFSFILGAVVGSFLNVCICRMPRDESVVSPPSHCPGCGYRIRWYDNIPLVSYLLLRGRCRGCREKISPRYPLV
ELLNALLSLALFLRFGPTLAFAVLFLFCSALVVITFIDLEHQIIPDEISLPGIVLGFIFSFFLRGHGWLNSLLGILLGGG
SLLLVAYGYQLVTGKEGMGGGDIKLLAMMGAFLGWKSIPFIIFVSSLAGSVIGVSLMLIQKKDSKLAIPFGPYLALGALL
YVFYGPRLISWYLTLGGLQG

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the pilin precursor during membrane translocation. Required for the assembly of type IV pili and for secretion of most proteins [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=256, Percent_Identity=35.546875, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789732, Length=146, Percent_Identity=36.3013698630137, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 28522; Mature: 28390

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQLLFSVFSFILGAVVGSFLNVCICRMPRDESVVSPPSHCPGCGYRIRWYDNIPLVSY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHH
LLLRGRCRGCREKISPRYPLVELLNALLSLALFLRFGPTLAFAVLFLFCSALVVITFIDL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHC
EHQIIPDEISLPGIVLGFIFSFFLRGHGWLNSLLGILLGGGSLLLVAYGYQLVTGKEGMG
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCEEEECCCCCC
GGDIKLLAMMGAFLGWKSIPFIIFVSSLAGSVIGVSLMLIQKKDSKLAIPFGPYLALGAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEECCCCHHHHHHHH
LYVFYGPRLISWYLTLGGLQG
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TPQLLFSVFSFILGAVVGSFLNVCICRMPRDESVVSPPSHCPGCGYRIRWYDNIPLVSY
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHH
LLLRGRCRGCREKISPRYPLVELLNALLSLALFLRFGPTLAFAVLFLFCSALVVITFIDL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHC
EHQIIPDEISLPGIVLGFIFSFFLRGHGWLNSLLGILLGGGSLLLVAYGYQLVTGKEGMG
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCEEEECCCCCC
GGDIKLLAMMGAFLGWKSIPFIIFVSSLAGSVIGVSLMLIQKKDSKLAIPFGPYLALGAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEECCCCHHHHHHHH
LYVFYGPRLISWYLTLGGLQG
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7905475 [H]