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The map label for this gene is ptsI [H]

Identifier: 118579417

GI number: 118579417

Start: 1022215

End: 1023981

Strand: Direct

Name: ptsI [H]

Synonym: Ppro_0981

Alternate gene names: 118579417

Gene position: 1022215-1023981 (Clockwise)

Preceding gene: 118579416

Following gene: 118579418

Centisome position: 25.5

GC content: 61.35

Gene sequence:

>1767_bases
ATGGATTCGGCGAAGAATAACAGCATGCTCAGGTTCAGCGGTATCGCCGCATCGCCCGGAGTTTCCGTGGGCAGACTGCG
TGTCGTCGACCGACGCCATATCTCCGTTGACGAGTACGCTGTCAGAGGGGAAGCCGTTACCGCCGAGGAGGAGCGCCTGA
AGGCGGGGATTGCCCGCACCAGGGACGAACTGAGCCAGCTCAAGGAAACGCTCAGGGAAACCGCCGGCGACGATCACCTC
TTTTTTATCGATACCCACCTGCTGATCCTGTCCGATGAACGTCTCTTCAGCGAAGCTGTGGCGCATATCACTACTGACCG
GATAAACGCCGAGGGCGCCCTGCGCCGGACACTCCAGAAGTACCGCGAATACTTTGCCGGCATAGACGACCCCTACCTAC
GCGAGCGGATCAACGATGTGGAAACGGTCATCGACAAGATCCTGCGCAACATTGCCGGTGAACCCCATGCGCCCATAGTT
CCCGATGGCGACCAGACCATCATCGTGGCTCACGACATCTCGCCCGCCGACATCCTGCAGATGGATCGCGAACGCATCAT
CGGATTCGTCACCGAGATAGGGGGGAGAACATCCCATGCGTCGATCCTGGCACGGGCCTTTGAGTTGCCGGCGGTGGTCG
GCGTGGAAGGGGTCGCCGACAAGCCGCTGGATGGAATGCCGGCCATCCTTGACGGTCTCAGCGGCACGGTGATCGTTAAC
CCCGACAGCAGTACCTTTCTCGAACATCTGCAGCGCAAACGGCACTATGAGTACGTTGAGTCCGGGCTGCTCAGGCTGGC
GGAACTGCCGCCCGAAACGCGGGACGGCCAGCGGATCCTGCTCAAGGGTAACGTTGAGGTGCCCGAGGAGGGACCGTCGG
TGCTGCGCCATGGCGGCGAAGGGGTCGGCCTGTACCGTACCGAAATGCTCTTCATGAACCGGAGGGAGATGCCCGAGGAG
GAAGAACAGTATCAGGCATATGCCGCCATGCAGCGCGCCGTTGAACCCCACCCCCTGACCATCAGGACACTGGACGCTGG
AGGCGACAAGCTGCTGGATGGACTGCAGCACCCCACGGAGCAGAATCCCGCCCTGGGTGTTCGCGCCATCCGGCTGGCGT
TGCGCATGCCCGAAGAGTTCAAAAAACAGCTGCGGGCGATTCTCAGGGTCAGCGCAGCCGGAGATGTGCGCATCATGTTT
CCCATGATCTCCTGTCTGGATGAGTTGCGTAGCGCCCGTGCCGTGCTGGAGCAGGCCAAGGCCGAACTGGATGACGCGGG
CATTCCCTTTGACCGCCACATCCAGGTAGGGATCATGATCGAGGTTCCCTCCGCGGTTGTGGTCTCCGATCTGCTGGCCA
GGGAATCGGATTTTTTCAGTGTCGGCACCAACGACCTGATCCAGTACACCCTGGCCATTGACCGCTCCAACGAGCATCTG
GTTTCGCTGTATCAGCCGCTCCATCCGGCGGTGCTCCGGTCCCTGCGCACGGTGGTGGAGGCCGCCCGCGGGGCCGGCAT
TCCCGCCTGTATCTGTGGTGAAATGGCCGGTGACCCGCTCTATCTGCCGGTACTGCTCGGGCTTGGCTTCGGAGAACTAT
CCATGGGCGCCGGTTCCATCCCACGGGTGAAGCAGATGCTGCGCCGTTGCGACATGAATCAGGCTGCCGAGGTCGCCAGC
GCCTGCTTCGCGTTTTCCACCGCCGAACAGGTTGACCTGTTCCTCAGGAATGCCATTGCCACCACGTTTTCCGATACCTT
GGATTGA

Upstream 100 bases:

>100_bases
ATCATGATGTTAGCCGCGTCGAAAGGGGTTACCGTCTGTTTGACCGTCGATGGCGCCGACGAGGCGGAGGCCATGGCAGC
CATGGGAGAGTTGATCGTCA

Downstream 100 bases:

>100_bases
GACGGGATCCCCATGACGCCGCAGCTCTTGTTTTCGGTCTTTTCCTTTATTCTTGGCGCTGTTGTCGGCTCCTTCCTCAA
TGTCTGCATCTGCCGCATGC

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MDSAKNNSMLRFSGIAASPGVSVGRLRVVDRRHISVDEYAVRGEAVTAEEERLKAGIARTRDELSQLKETLRETAGDDHL
FFIDTHLLILSDERLFSEAVAHITTDRINAEGALRRTLQKYREYFAGIDDPYLRERINDVETVIDKILRNIAGEPHAPIV
PDGDQTIIVAHDISPADILQMDRERIIGFVTEIGGRTSHASILARAFELPAVVGVEGVADKPLDGMPAILDGLSGTVIVN
PDSSTFLEHLQRKRHYEYVESGLLRLAELPPETRDGQRILLKGNVEVPEEGPSVLRHGGEGVGLYRTEMLFMNRREMPEE
EEQYQAYAAMQRAVEPHPLTIRTLDAGGDKLLDGLQHPTEQNPALGVRAIRLALRMPEEFKKQLRAILRVSAAGDVRIMF
PMISCLDELRSARAVLEQAKAELDDAGIPFDRHIQVGIMIEVPSAVVVSDLLARESDFFSVGTNDLIQYTLAIDRSNEHL
VSLYQPLHPAVLRSLRTVVEAARGAGIPACICGEMAGDPLYLPVLLGLGFGELSMGAGSIPRVKQMLRRCDMNQAAEVAS
ACFAFSTAEQVDLFLRNAIATTFSDTLD

Sequences:

>Translated_588_residues
MDSAKNNSMLRFSGIAASPGVSVGRLRVVDRRHISVDEYAVRGEAVTAEEERLKAGIARTRDELSQLKETLRETAGDDHL
FFIDTHLLILSDERLFSEAVAHITTDRINAEGALRRTLQKYREYFAGIDDPYLRERINDVETVIDKILRNIAGEPHAPIV
PDGDQTIIVAHDISPADILQMDRERIIGFVTEIGGRTSHASILARAFELPAVVGVEGVADKPLDGMPAILDGLSGTVIVN
PDSSTFLEHLQRKRHYEYVESGLLRLAELPPETRDGQRILLKGNVEVPEEGPSVLRHGGEGVGLYRTEMLFMNRREMPEE
EEQYQAYAAMQRAVEPHPLTIRTLDAGGDKLLDGLQHPTEQNPALGVRAIRLALRMPEEFKKQLRAILRVSAAGDVRIMF
PMISCLDELRSARAVLEQAKAELDDAGIPFDRHIQVGIMIEVPSAVVVSDLLARESDFFSVGTNDLIQYTLAIDRSNEHL
VSLYQPLHPAVLRSLRTVVEAARGAGIPACICGEMAGDPLYLPVLLGLGFGELSMGAGSIPRVKQMLRRCDMNQAAEVAS
ACFAFSTAEQVDLFLRNAIATTFSDTLD
>Mature_588_residues
MDSAKNNSMLRFSGIAASPGVSVGRLRVVDRRHISVDEYAVRGEAVTAEEERLKAGIARTRDELSQLKETLRETAGDDHL
FFIDTHLLILSDERLFSEAVAHITTDRINAEGALRRTLQKYREYFAGIDDPYLRERINDVETVIDKILRNIAGEPHAPIV
PDGDQTIIVAHDISPADILQMDRERIIGFVTEIGGRTSHASILARAFELPAVVGVEGVADKPLDGMPAILDGLSGTVIVN
PDSSTFLEHLQRKRHYEYVESGLLRLAELPPETRDGQRILLKGNVEVPEEGPSVLRHGGEGVGLYRTEMLFMNRREMPEE
EEQYQAYAAMQRAVEPHPLTIRTLDAGGDKLLDGLQHPTEQNPALGVRAIRLALRMPEEFKKQLRAILRVSAAGDVRIMF
PMISCLDELRSARAVLEQAKAELDDAGIPFDRHIQVGIMIEVPSAVVVSDLLARESDFFSVGTNDLIQYTLAIDRSNEHL
VSLYQPLHPAVLRSLRTVVEAARGAGIPACICGEMAGDPLYLPVLLGLGFGELSMGAGSIPRVKQMLRRCDMNQAAEVAS
ACFAFSTAEQVDLFLRNAIATTFSDTLD

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=562, Percent_Identity=39.3238434163701, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1789193, Length=531, Percent_Identity=36.5348399246704, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1788726, Length=573, Percent_Identity=34.2059336823735, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI48994992, Length=495, Percent_Identity=35.7575757575758, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI1787994, Length=409, Percent_Identity=25.6723716381418, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64835; Mature: 64835

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSAKNNSMLRFSGIAASPGVSVGRLRVVDRRHISVDEYAVRGEAVTAEEERLKAGIART
CCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHH
RDELSQLKETLRETAGDDHLFFIDTHLLILSDERLFSEAVAHITTDRINAEGALRRTLQK
HHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHH
YREYFAGIDDPYLRERINDVETVIDKILRNIAGEPHAPIVPDGDQTIIVAHDISPADILQ
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHH
MDRERIIGFVTEIGGRTSHASILARAFELPAVVGVEGVADKPLDGMPAILDGLSGTVIVN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCCCCHHHHCCCCCEEEEC
PDSSTFLEHLQRKRHYEYVESGLLRLAELPPETRDGQRILLKGNVEVPEEGPSVLRHGGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCC
GVGLYRTEMLFMNRREMPEEEEQYQAYAAMQRAVEPHPLTIRTLDAGGDKLLDGLQHPTE
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCCC
QNPALGVRAIRLALRMPEEFKKQLRAILRVSAAGDVRIMFPMISCLDELRSARAVLEQAK
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
AELDDAGIPFDRHIQVGIMIEVPSAVVVSDLLARESDFFSVGTNDLIQYTLAIDRSNEHL
HHHCCCCCCCCCEEEEEEEEECCHHHHHHHHHHCCCCCEECCCHHHEEEEEEEECCCHHH
VSLYQPLHPAVLRSLRTVVEAARGAGIPACICGEMAGDPLYLPVLLGLGFGELSMGAGSI
HHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHCCCCHHCCCCCCC
PRVKQMLRRCDMNQAAEVASACFAFSTAEQVDLFLRNAIATTFSDTLD
HHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDSAKNNSMLRFSGIAASPGVSVGRLRVVDRRHISVDEYAVRGEAVTAEEERLKAGIART
CCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHH
RDELSQLKETLRETAGDDHLFFIDTHLLILSDERLFSEAVAHITTDRINAEGALRRTLQK
HHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHH
YREYFAGIDDPYLRERINDVETVIDKILRNIAGEPHAPIVPDGDQTIIVAHDISPADILQ
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHH
MDRERIIGFVTEIGGRTSHASILARAFELPAVVGVEGVADKPLDGMPAILDGLSGTVIVN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCCCCHHHHCCCCCEEEEC
PDSSTFLEHLQRKRHYEYVESGLLRLAELPPETRDGQRILLKGNVEVPEEGPSVLRHGGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCC
GVGLYRTEMLFMNRREMPEEEEQYQAYAAMQRAVEPHPLTIRTLDAGGDKLLDGLQHPTE
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCCC
QNPALGVRAIRLALRMPEEFKKQLRAILRVSAAGDVRIMFPMISCLDELRSARAVLEQAK
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
AELDDAGIPFDRHIQVGIMIEVPSAVVVSDLLARESDFFSVGTNDLIQYTLAIDRSNEHL
HHHCCCCCCCCCEEEEEEEEECCHHHHHHHHHHCCCCCEECCCHHHEEEEEEEECCCHHH
VSLYQPLHPAVLRSLRTVVEAARGAGIPACICGEMAGDPLYLPVLLGLGFGELSMGAGSI
HHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHCCCCHHCCCCCCC
PRVKQMLRRCDMNQAAEVASACFAFSTAEQVDLFLRNAIATTFSDTLD
HHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA