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The map label for this gene is oprM [H]

Identifier: 118579204

GI number: 118579204

Start: 812739

End: 814181

Strand: Direct

Name: oprM [H]

Synonym: Ppro_0766

Alternate gene names: 118579204

Gene position: 812739-814181 (Clockwise)

Preceding gene: 118579203

Following gene: 118579205

Centisome position: 20.28

GC content: 69.16

Gene sequence:

>1443_bases
GTGGGGCCTGATTTCAGGCGGCCGGAACAGGCGCTTCCTGTCGGCTATACCGCACCTGCTCTGCCGAAGGTTCATCCGGC
GGTGGGTGGCGCTGCCGGCGAGACGCAGGCAGTGGTTCCCGGCCGGGACCTGCCCGCCCAGTGGTGGCGGCTGTTCCGCA
ATGCCGAGCTCGACCGTCTGATCCGCCAGGCATTGGACGATAGTCCCACCTTGGCCGCTGCCGGGGCGGCCCTGCGCCAG
GCCGAGGAGAACCTGCGCGCCCGGAGCGGGGCGGTATACTATCCCGCCCTGGACGCTTCCCTCTCCGGGGCGCGCCAGAA
GGTGTCAAGCGCCGCCAGCGGCCAGCCGGGAACGGGTGGTTTTACCTACGGGCTGTACAACGCCTCGCTGGTGCTCTCCT
ACGACCTTGACCTGTTCGGCGGCGGCCGCCGCGAACTGGAGGAGTTGCGCTCACAGGTGGATTACCAGCGTTTCCAGCTG
GAGGCCGCCCATCTCTCCCTGACCGCCAACATCGTCACCACGGTGGTGAGGGAGGCCTCCCTGAGGGCGCAACTGGCAAC
CACCCGTGAGATGCTGGCGGTACAGGAACAGCTGCGCGACCTGGTGGCGCGGCAGCTCCAGCTGGGGGGGACGACCCGCG
TCGAACTGCTCAGCCAGGAGAGCCAGGTGGCCCAGTTGCGGGCCAGCCTGCCCCTGCTGGAAAAGGAGCTTGCCTGGACC
CGCAACCAGCTGGCGCAGTACTGCGCCAGGTTCCCCAGCCAGGCCGGATTGCCGGAATTCAGCCTGGACCAGCTGCTGCT
GCCGCCGGAACTGCCGCTCTCCCTCCCCTCGTCCCTGGCCCGGCAGCGCCCGGATATCCGCGCTGCGGAAGAACTGCTCC
ACGCAGCCAGCGCCCGTATCGGCGTTGCCACGGCCAACCTCTACCCCCAGATCACCCTCAGCGGCGGCGTGGGGACCCAG
GCCGCCACCCTGGGCAAGCTGCTCTCCAGCGGCAGCCCCATCTGGAGCCTGGGCGCCGGCCTGCTGCAGCCCCTGTTCCG
TGGCGGCGAGCTGACCGCCAGGCGCCGGGCCGCGATCGCCGCCTATGACCAGGCAACGGCGCAGTACCGGGAGACGATTC
TGAAATCGTTTCAGAGTGTGGCCGACGTACTGCAGGCCCTGGATTCGGATGCGACCGCACTCGTGGCCCAGGCCGAAAGC
GAGCGCACAGCCCGTGAGACCCTCACGCTGACCCGTCGGCAGTATGAAATCGGTGCGACAAACTACCTGCTGCTGCTCAA
TGCCCAGCGTCAGCAGCAGCAGGCCAGCATTGCCCTGATCCAGGCCCGGGCAGCCCGTCTGGCGGACAGCGCCGCGCTCT
TCCAGGCCCTGGGGGGCGGATGGTGGAACCGGGAAGCCGGAGCCGATGCCGCTCAACCCGCGGAGCAGGAGGAGCGGCGA
TGA

Upstream 100 bases:

>100_bases
AAGGCGTTGAGTGATGGGCATTGTGCTAAACAGAGTTCTCCGTAACGGAAGACGGGTTGTGTTCGCTCTGGCAGCTGCCG
CTGCGCTGGCGGGGTGTGCC

Downstream 100 bases:

>100_bases
GTCTTCCGAACGCCCTGCTCCCTCACGCTTCCTCTCCACCCTATTGCGGAGTTATACAACCATGAATAAACGAGTCCTGT
TTGCCATACTTGGCCTCTTG

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MGPDFRRPEQALPVGYTAPALPKVHPAVGGAAGETQAVVPGRDLPAQWWRLFRNAELDRLIRQALDDSPTLAAAGAALRQ
AEENLRARSGAVYYPALDASLSGARQKVSSAASGQPGTGGFTYGLYNASLVLSYDLDLFGGGRRELEELRSQVDYQRFQL
EAAHLSLTANIVTTVVREASLRAQLATTREMLAVQEQLRDLVARQLQLGGTTRVELLSQESQVAQLRASLPLLEKELAWT
RNQLAQYCARFPSQAGLPEFSLDQLLLPPELPLSLPSSLARQRPDIRAAEELLHAASARIGVATANLYPQITLSGGVGTQ
AATLGKLLSSGSPIWSLGAGLLQPLFRGGELTARRRAAIAAYDQATAQYRETILKSFQSVADVLQALDSDATALVAQAES
ERTARETLTLTRRQYEIGATNYLLLLNAQRQQQQASIALIQARAARLADSAALFQALGGGWWNREAGADAAQPAEQEERR

Sequences:

>Translated_480_residues
MGPDFRRPEQALPVGYTAPALPKVHPAVGGAAGETQAVVPGRDLPAQWWRLFRNAELDRLIRQALDDSPTLAAAGAALRQ
AEENLRARSGAVYYPALDASLSGARQKVSSAASGQPGTGGFTYGLYNASLVLSYDLDLFGGGRRELEELRSQVDYQRFQL
EAAHLSLTANIVTTVVREASLRAQLATTREMLAVQEQLRDLVARQLQLGGTTRVELLSQESQVAQLRASLPLLEKELAWT
RNQLAQYCARFPSQAGLPEFSLDQLLLPPELPLSLPSSLARQRPDIRAAEELLHAASARIGVATANLYPQITLSGGVGTQ
AATLGKLLSSGSPIWSLGAGLLQPLFRGGELTARRRAAIAAYDQATAQYRETILKSFQSVADVLQALDSDATALVAQAES
ERTARETLTLTRRQYEIGATNYLLLLNAQRQQQQASIALIQARAARLADSAALFQALGGGWWNREAGADAAQPAEQEERR
>Mature_479_residues
GPDFRRPEQALPVGYTAPALPKVHPAVGGAAGETQAVVPGRDLPAQWWRLFRNAELDRLIRQALDDSPTLAAAGAALRQA
EENLRARSGAVYYPALDASLSGARQKVSSAASGQPGTGGFTYGLYNASLVLSYDLDLFGGGRRELEELRSQVDYQRFQLE
AAHLSLTANIVTTVVREASLRAQLATTREMLAVQEQLRDLVARQLQLGGTTRVELLSQESQVAQLRASLPLLEKELAWTR
NQLAQYCARFPSQAGLPEFSLDQLLLPPELPLSLPSSLARQRPDIRAAEELLHAASARIGVATANLYPQITLSGGVGTQA
ATLGKLLSSGSPIWSLGAGLLQPLFRGGELTARRRAAIAAYDQATAQYRETILKSFQSVADVLQALDSDATALVAQAESE
RTARETLTLTRRQYEIGATNYLLLLNAQRQQQQASIALIQARAARLADSAALFQALGGGWWNREAGADAAQPAEQEERR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=464, Percent_Identity=27.1551724137931, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI2367348, Length=439, Percent_Identity=24.373576309795, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51823; Mature: 51692

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPDFRRPEQALPVGYTAPALPKVHPAVGGAAGETQAVVPGRDLPAQWWRLFRNAELDRL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHCCCHHHH
IRQALDDSPTLAAAGAALRQAEENLRARSGAVYYPALDASLSGARQKVSSAASGQPGTGG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCC
FTYGLYNASLVLSYDLDLFGGGRRELEELRSQVDYQRFQLEAAHLSLTANIVTTVVREAS
EEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRAQLATTREMLAVQEQLRDLVARQLQLGGTTRVELLSQESQVAQLRASLPLLEKELAWT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RNQLAQYCARFPSQAGLPEFSLDQLLLPPELPLSLPSSLARQRPDIRAAEELLHAASARI
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
GVATANLYPQITLSGGVGTQAATLGKLLSSGSPIWSLGAGLLQPLFRGGELTARRRAAIA
EEEEECCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
AYDQATAQYRETILKSFQSVADVLQALDSDATALVAQAESERTARETLTLTRRQYEIGAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NYLLLLNAQRQQQQASIALIQARAARLADSAALFQALGGGWWNREAGADAAQPAEQEERR
CEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
GPDFRRPEQALPVGYTAPALPKVHPAVGGAAGETQAVVPGRDLPAQWWRLFRNAELDRL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHCCCHHHH
IRQALDDSPTLAAAGAALRQAEENLRARSGAVYYPALDASLSGARQKVSSAASGQPGTGG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCC
FTYGLYNASLVLSYDLDLFGGGRRELEELRSQVDYQRFQLEAAHLSLTANIVTTVVREAS
EEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRAQLATTREMLAVQEQLRDLVARQLQLGGTTRVELLSQESQVAQLRASLPLLEKELAWT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RNQLAQYCARFPSQAGLPEFSLDQLLLPPELPLSLPSSLARQRPDIRAAEELLHAASARI
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
GVATANLYPQITLSGGVGTQAATLGKLLSSGSPIWSLGAGLLQPLFRGGELTARRRAAIA
EEEEECCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
AYDQATAQYRETILKSFQSVADVLQALDSDATALVAQAESERTARETLTLTRRQYEIGAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NYLLLLNAQRQQQQASIALIQARAARLADSAALFQALGGGWWNREAGADAAQPAEQEERR
CEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]