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The map label for this gene is adhE [H]

Identifier: 118579194

GI number: 118579194

Start: 800306

End: 801661

Strand: Reverse

Name: adhE [H]

Synonym: Ppro_0756

Alternate gene names: 118579194

Gene position: 801661-800306 (Counterclockwise)

Preceding gene: 118579217

Following gene: 118579192

Centisome position: 20.0

GC content: 57.15

Gene sequence:

>1356_bases
ATGGCAGACGTAGTTAAAGTGATTGATGAGTTGGTAGCAAAGGCAACGAAGGCAGCTCAAATCTACAAAACCTTCACCCA
GGAGCAGGTTGACAAAATTTGTGCCGCCATGGACGCAGTCAGCGTCGCCAACGAGGTAATCCTTGGCGAAGAAGCTGTAG
CGGAAACCGGCATCGGCCGCGCTGACCACAAAGCCATCAAGAACCACCTTGGCGCCCACGTTGTTGCTGAATGGCAAAAA
GATCAAAAATCCGTTGGTATTATCAGGGAAGAAGAAGGCGTAAAGTACATCGCCGAACCTTTCGGTGTTATCGCCGCCGC
TACACCGACCACAAACCCGACTTCCACCGTTATGTTCAAGTCGCTGATCGCCCTCAAGGCCCGTAACTCCGTCATCTTCG
CTGCCCACCCACGCGCACAGAAGTGCAGCGTCCACGCAGCACAGTTGATGCGTGATGCCGCCATCAGCGCCGGTGCACCC
GCTGACATCATCCAGTGGATCGATGAACCCTCCATCGAAGCAACCAACGCCCTGTTCAAGCATCCCGGCGTAAAACTGGT
CCTGGCCACCGGCGGCGGCGCCATGGTTAAATCGGCGTACAGCTCCGGCCACCCCGCCATCGGCGTCGGCCCCGGCAACA
CCCCGGTATTCATCTCCAAGTCGGCCAAACTCAGCGTTGCCATCAACAACGTTATCGCCTCCAAGACCTTCGACAATGGC
ACCATCTGCTCCTCCGACCAGTCCATGATCTTTGACGATCCGGCCGTCTGCAAAGAGGGCGTGAAGATGATGGTCGAGCG
CGGAGCCTATCTCGTCAACGACGAAGAGAAAGCCAAGCTGGAAGCGGTCATGTTCGACAAGGAACGCGGCGTTCCCGCCA
CCGCCATCGTCGGCAAATCACCCCAGTTCATCGCCAAGCTGGCCGGTTTTGATATTCCTGAAGACGCCAAACTGCTGTTG
GTGCCCCTCAAGTGCATCGGACCCGAAGACTGGTTCAGCCATGAGAAACTCTCCCCAGTTCTGGGCTATATCGCCTACAA
CGGCACCGACGAGGCCATTGCCGCAGCAAAATCCCAGCTGCTGTGGGGCGGCGCCGGACATACTTCGGTTGTTCACTCCC
AGGACCAGGCCATTCTGGACAGGTATGCCATGGAAATCCCCGCCAACCGTTTGCTGCTCAACCAGCCTGCGGTTCACGGC
TCCGTCGGCCTGATCTACAACAAACTGCCTCCTTCACTGACTCTGGGTTGCGGCACCGACGGCAGCAACTACCTGGGCAA
CAACATCAGTTTCCGCGACCTGATGAGCATCAAGGCGGTTGCACAGCGTATGGTTGACTACGAGCGCGACGAATAA

Upstream 100 bases:

>100_bases
ACGCCACGTGCAAAAATCACATTGTTATATTTGGCAACAAAATGCTTCGCACAGTCTAACGCTGTTGCGTGCAGTAACAC
TATTCCATGGGAGGTATGTA

Downstream 100 bases:

>100_bases
GAACATCCTCCCCAGCAACGAAAAAACGTTGCAATAAAAAACCCCCCTGTCCGGACGACCCGGGTAGGGGGGTTTTACAT
GTCAACTGCTCAACGACCTA

Product: acetaldehyde dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MADVVKVIDELVAKATKAAQIYKTFTQEQVDKICAAMDAVSVANEVILGEEAVAETGIGRADHKAIKNHLGAHVVAEWQK
DQKSVGIIREEEGVKYIAEPFGVIAAATPTTNPTSTVMFKSLIALKARNSVIFAAHPRAQKCSVHAAQLMRDAAISAGAP
ADIIQWIDEPSIEATNALFKHPGVKLVLATGGGAMVKSAYSSGHPAIGVGPGNTPVFISKSAKLSVAINNVIASKTFDNG
TICSSDQSMIFDDPAVCKEGVKMMVERGAYLVNDEEKAKLEAVMFDKERGVPATAIVGKSPQFIAKLAGFDIPEDAKLLL
VPLKCIGPEDWFSHEKLSPVLGYIAYNGTDEAIAAAKSQLLWGGAGHTSVVHSQDQAILDRYAMEIPANRLLLNQPAVHG
SVGLIYNKLPPSLTLGCGTDGSNYLGNNISFRDLMSIKAVAQRMVDYERDE

Sequences:

>Translated_451_residues
MADVVKVIDELVAKATKAAQIYKTFTQEQVDKICAAMDAVSVANEVILGEEAVAETGIGRADHKAIKNHLGAHVVAEWQK
DQKSVGIIREEEGVKYIAEPFGVIAAATPTTNPTSTVMFKSLIALKARNSVIFAAHPRAQKCSVHAAQLMRDAAISAGAP
ADIIQWIDEPSIEATNALFKHPGVKLVLATGGGAMVKSAYSSGHPAIGVGPGNTPVFISKSAKLSVAINNVIASKTFDNG
TICSSDQSMIFDDPAVCKEGVKMMVERGAYLVNDEEKAKLEAVMFDKERGVPATAIVGKSPQFIAKLAGFDIPEDAKLLL
VPLKCIGPEDWFSHEKLSPVLGYIAYNGTDEAIAAAKSQLLWGGAGHTSVVHSQDQAILDRYAMEIPANRLLLNQPAVHG
SVGLIYNKLPPSLTLGCGTDGSNYLGNNISFRDLMSIKAVAQRMVDYERDE
>Mature_450_residues
ADVVKVIDELVAKATKAAQIYKTFTQEQVDKICAAMDAVSVANEVILGEEAVAETGIGRADHKAIKNHLGAHVVAEWQKD
QKSVGIIREEEGVKYIAEPFGVIAAATPTTNPTSTVMFKSLIALKARNSVIFAAHPRAQKCSVHAAQLMRDAAISAGAPA
DIIQWIDEPSIEATNALFKHPGVKLVLATGGGAMVKSAYSSGHPAIGVGPGNTPVFISKSAKLSVAINNVIASKTFDNGT
ICSSDQSMIFDDPAVCKEGVKMMVERGAYLVNDEEKAKLEAVMFDKERGVPATAIVGKSPQFIAKLAGFDIPEDAKLLLV
PLKCIGPEDWFSHEKLSPVLGYIAYNGTDEAIAAAKSQLLWGGAGHTSVVHSQDQAILDRYAMEIPANRLLLNQPAVHGS
VGLIYNKLPPSLTLGCGTDGSNYLGNNISFRDLMSIKAVAQRMVDYERDE

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787493, Length=442, Percent_Identity=43.4389140271493, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI1788797, Length=359, Percent_Identity=31.7548746518106, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 48065; Mature: 47933

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVVKVIDELVAKATKAAQIYKTFTQEQVDKICAAMDAVSVANEVILGEEAVAETGIGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCH
ADHKAIKNHLGAHVVAEWQKDQKSVGIIREEEGVKYIAEPFGVIAAATPTTNPTSTVMFK
HHHHHHHHHCCCHHHHHHHHCCHHCCCEECCCCCHHHHCCCCEEEECCCCCCCHHHHHHH
SLIALKARNSVIFAAHPRAQKCSVHAAQLMRDAAISAGAPADIIQWIDEPSIEATNALFK
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHH
HPGVKLVLATGGGAMVKSAYSSGHPAIGVGPGNTPVFISKSAKLSVAINNVIASKTFDNG
CCCEEEEEEECCCHHHHHHHCCCCCEEEECCCCCCEEEECCCEEEEEEHHHHHHCCCCCC
TICSSDQSMIFDDPAVCKEGVKMMVERGAYLVNDEEKAKLEAVMFDKERGVPATAIVGKS
CEECCCCCEEECCHHHHHHHHHHHHHCCCEEECCCHHHHEEHEEEECCCCCCEEEEECCC
PQFIAKLAGFDIPEDAKLLLVPLKCIGPEDWFSHEKLSPVLGYIAYNGTDEAIAAAKSQL
HHHHHHHHCCCCCCCCEEEEEEHHHCCCHHHCCCHHHHHHHHHEEECCCHHHHHHHHHHH
LWGGAGHTSVVHSQDQAILDRYAMEIPANRLLLNQPAVHGSVGLIYNKLPPSLTLGCGTD
EECCCCCCCEEECCHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCCCEEEECCCC
GSNYLGNNISFRDLMSIKAVAQRMVDYERDE
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ADVVKVIDELVAKATKAAQIYKTFTQEQVDKICAAMDAVSVANEVILGEEAVAETGIGR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCH
ADHKAIKNHLGAHVVAEWQKDQKSVGIIREEEGVKYIAEPFGVIAAATPTTNPTSTVMFK
HHHHHHHHHCCCHHHHHHHHCCHHCCCEECCCCCHHHHCCCCEEEECCCCCCCHHHHHHH
SLIALKARNSVIFAAHPRAQKCSVHAAQLMRDAAISAGAPADIIQWIDEPSIEATNALFK
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHH
HPGVKLVLATGGGAMVKSAYSSGHPAIGVGPGNTPVFISKSAKLSVAINNVIASKTFDNG
CCCEEEEEEECCCHHHHHHHCCCCCEEEECCCCCCEEEECCCEEEEEEHHHHHHCCCCCC
TICSSDQSMIFDDPAVCKEGVKMMVERGAYLVNDEEKAKLEAVMFDKERGVPATAIVGKS
CEECCCCCEEECCHHHHHHHHHHHHHCCCEEECCCHHHHEEHEEEECCCCCCEEEEECCC
PQFIAKLAGFDIPEDAKLLLVPLKCIGPEDWFSHEKLSPVLGYIAYNGTDEAIAAAKSQL
HHHHHHHHCCCCCCCCEEEEEEHHHCCCHHHCCCHHHHHHHHHEEECCCHHHHHHHHHHH
LWGGAGHTSVVHSQDQAILDRYAMEIPANRLLLNQPAVHGSVGLIYNKLPPSLTLGCGTD
EECCCCCCCEEECCHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCCCEEEECCCC
GSNYLGNNISFRDLMSIKAVAQRMVDYERDE
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]