Click here to switch to the map view.

The map label for this gene is rpoA

Identifier: 118579145

GI number: 118579145

Start: 735699

End: 736721

Strand: Direct

Name: rpoA

Synonym: Ppro_0706

Alternate gene names: 118579145

Gene position: 735699-736721 (Clockwise)

Preceding gene: 118579144

Following gene: 118579146

Centisome position: 18.36

GC content: 41.06

Gene sequence:

>1023_bases
ATGTATAAAAACTGGCGCGATCTTATCAGACCTAAGCAATTACAGTTTGAAAAAGAGTCGCTTTCAGATACATACGGTAA
ATTTTTTGCAGAACCTTTTGAACGTGGTTTTGCTACAACTTTGGGAAATTCTTTGCGGAGAATACTTCTTTCTTCACTTC
AGGGTTCCGCAATTACTTCAGTTCGTATAAAGGGTGTTTTGCACGAATTCAGTGCCATCCAAGGTGTTACTGAAGATGTT
ACAGATATCATTCTTAATCTTAAGGGCGTTCGTCTGAAGCTTCACTCTGTTGTTCAGGCTACTATACGTGTAGTCCATAC
CGGTGAGGGTGTCGTAAAAGCTGGCGACTTTGTCGTCGGTCATAACGTTGAGATAATGAATCCCGAACACCATATTGCAA
CATGCGGCAAAGATGCTCATTTTGAAATGGATATGACTGTCAAGATGGGTAAGGGTTATGTCTCGGCTGATAAAAACCGC
GATGAAAAAGCCCCGGTTGGTACAATTCCCATTGACTCGATATTTTCTCCGATCAAGAAAGTTAATTTTACCATTTCCAA
CGCTCGTGTTGGTCAAATGACAGACTATGACAAACTGACCTTAGAGATTTGGACGGATGGAAGTGTCAAGCCTGATGATG
CTCTGGCCTATTCCGCAAAAATTATGAAAGAGCAGTTATCTATTTTCATTAATTTTGATGAAGAAGCTGAGCCTCAGGCT
CCTGAAGAGTCACAGGATGAAATAGATAAGATAAACGAGAATCTCTACCGTACGGTTGAGGAACTTGAACTTTCTGTACG
ATCAGCCAATTGCCTTAAGAACGCCGGTATCAAACTTATTGGCGAACTCGTTTCTAAGACTGAGGCTGAAATGTTAAAAA
CACAGAATTTCGGTCGTAAGTCTCTGAACGAAATCAAAGATATTTTGAGTGATATGGGACTTACGTTTGGTATGAAGCTT
GACTCTTTTCCCGAGCCTGATATGTTACGTCGCTTGCGTGGAGAGCAGAACGAAGAAGAATAA

Upstream 100 bases:

>100_bases
CAACTGGTCGTCGAACTCTATTCAAAATAAGTAATGCTGACTGAATACGAGTGTTTTCAGTAACTCCCAGCACCGGGCGT
AATCTCCGGAGGAGGTAGTA

Downstream 100 bases:

>100_bases
ATCCTTTTATTTAAGCGTATTTTGAGAAAGGAACCGATATATGCGGCATTTAAAATCAGGAAGGCGTCTTGGAAGAAAAA
CGAGTCATCGTGAGGCCATG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MYKNWRDLIRPKQLQFEKESLSDTYGKFFAEPFERGFATTLGNSLRRILLSSLQGSAITSVRIKGVLHEFSAIQGVTEDV
TDIILNLKGVRLKLHSVVQATIRVVHTGEGVVKAGDFVVGHNVEIMNPEHHIATCGKDAHFEMDMTVKMGKGYVSADKNR
DEKAPVGTIPIDSIFSPIKKVNFTISNARVGQMTDYDKLTLEIWTDGSVKPDDALAYSAKIMKEQLSIFINFDEEAEPQA
PEESQDEIDKINENLYRTVEELELSVRSANCLKNAGIKLIGELVSKTEAEMLKTQNFGRKSLNEIKDILSDMGLTFGMKL
DSFPEPDMLRRLRGEQNEEE

Sequences:

>Translated_340_residues
MYKNWRDLIRPKQLQFEKESLSDTYGKFFAEPFERGFATTLGNSLRRILLSSLQGSAITSVRIKGVLHEFSAIQGVTEDV
TDIILNLKGVRLKLHSVVQATIRVVHTGEGVVKAGDFVVGHNVEIMNPEHHIATCGKDAHFEMDMTVKMGKGYVSADKNR
DEKAPVGTIPIDSIFSPIKKVNFTISNARVGQMTDYDKLTLEIWTDGSVKPDDALAYSAKIMKEQLSIFINFDEEAEPQA
PEESQDEIDKINENLYRTVEELELSVRSANCLKNAGIKLIGELVSKTEAEMLKTQNFGRKSLNEIKDILSDMGLTFGMKL
DSFPEPDMLRRLRGEQNEEE
>Mature_340_residues
MYKNWRDLIRPKQLQFEKESLSDTYGKFFAEPFERGFATTLGNSLRRILLSSLQGSAITSVRIKGVLHEFSAIQGVTEDV
TDIILNLKGVRLKLHSVVQATIRVVHTGEGVVKAGDFVVGHNVEIMNPEHHIATCGKDAHFEMDMTVKMGKGYVSADKNR
DEKAPVGTIPIDSIFSPIKKVNFTISNARVGQMTDYDKLTLEIWTDGSVKPDDALAYSAKIMKEQLSIFINFDEEAEPQA
PEESQDEIDKINENLYRTVEELELSVRSANCLKNAGIKLIGELVSKTEAEMLKTQNFGRKSLNEIKDILSDMGLTFGMKL
DSFPEPDMLRRLRGEQNEEE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=328, Percent_Identity=41.4634146341463, Blast_Score=265, Evalue=3e-72,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_PELPD (A1ALW7)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_900395.1
- ProteinModelPortal:   A1ALW7
- STRING:   A1ALW7
- GeneID:   4571505
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_0706
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   A1ALW7
- ProtClustDB:   PRK05182
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 38204; Mature: 38204

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKNWRDLIRPKQLQFEKESLSDTYGKFFAEPFERGFATTLGNSLRRILLSSLQGSAITS
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VRIKGVLHEFSAIQGVTEDVTDIILNLKGVRLKLHSVVQATIRVVHTGEGVVKAGDFVVG
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEECCCEEEE
HNVEIMNPEHHIATCGKDAHFEMDMTVKMGKGYVSADKNRDEKAPVGTIPIDSIFSPIKK
CCEEEECCCCHHEECCCCCCEEEEEEEEECCCEEECCCCCCCCCCCCCCCHHHHHHHHHH
VNFTISNARVGQMTDYDKLTLEIWTDGSVKPDDALAYSAKIMKEQLSIFINFDEEAEPQA
HEEEECCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCC
PEESQDEIDKINENLYRTVEELELSVRSANCLKNAGIKLIGELVSKTEAEMLKTQNFGRK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHH
SLNEIKDILSDMGLTFGMKLDSFPEPDMLRRLRGEQNEEE
HHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MYKNWRDLIRPKQLQFEKESLSDTYGKFFAEPFERGFATTLGNSLRRILLSSLQGSAITS
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VRIKGVLHEFSAIQGVTEDVTDIILNLKGVRLKLHSVVQATIRVVHTGEGVVKAGDFVVG
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEECCCEEEE
HNVEIMNPEHHIATCGKDAHFEMDMTVKMGKGYVSADKNRDEKAPVGTIPIDSIFSPIKK
CCEEEECCCCHHEECCCCCCEEEEEEEEECCCEEECCCCCCCCCCCCCCCHHHHHHHHHH
VNFTISNARVGQMTDYDKLTLEIWTDGSVKPDDALAYSAKIMKEQLSIFINFDEEAEPQA
HEEEECCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCC
PEESQDEIDKINENLYRTVEELELSVRSANCLKNAGIKLIGELVSKTEAEMLKTQNFGRK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHH
SLNEIKDILSDMGLTFGMKLDSFPEPDMLRRLRGEQNEEE
HHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA