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The map label for this gene is fusA [H]

Identifier: 118579116

GI number: 118579116

Start: 719027

End: 721105

Strand: Direct

Name: fusA [H]

Synonym: Ppro_0677

Alternate gene names: 118579116

Gene position: 719027-721105 (Clockwise)

Preceding gene: 118579115

Following gene: 118579117

Centisome position: 17.94

GC content: 50.02

Gene sequence:

>2079_bases
GTGCCCCGTTTAGCAACGCTTGATAAATACAGAAACATCGGCATCATGGCCCATATTGACGCGGGCAAGACTACGACAAC
CGAGCGTATTCTCTACTACACCGGTGTCTCGCATAAGATTGGTGAGGTTCATGAGGGCGCGGCCACCATGGACTGGATGG
AGCAGGAGCAGGAGAGGGGTATTACCATTACATCCGCTGCTACTACCTGTTCGTGGCGTGACCATCGCATAAACATCATT
GACACCCCCGGACATGTGGACTTCACGATCGAGGTAGAGCGCTCGCTGCGTGTGCTCGATGGCGCGGTCGCCGTATTCTG
TTCTGTAGGTGGTGTTGAGCCACAGTCAGAGACTGTGTGGCGTCAAGCTGATAAATATGGCGTTCCCCGCATCGCCTTTA
TCAATAAGATGGATCGGGTCGGCGCGGATTTTTTTCGCGGCGTCCAGATGATCAGGGATCGCCTCAAAGCGAATCCTCTG
CCGATCCAGATACCGATCGGCAAGGAAGAGAATTTCAAGGGTGTAGTTGACCTTGTGCGCATGAAAGCTGTTATATGGGA
AGAAGAAGCGCTTGGTGCCAACTACCATGAGGAAGAGATTCCTGCTGATCTGCTTGTCGAAGCTCAGGAGTACCGTGACA
AGATGATCGAAGAGATTTCTAGTCATGATGATACTCTCATGGAGAAGTATCTGAGTGGCGAGGAATTGACCGAGCAGGAA
ATCATGGACGCCATCAGAACCTGTACCATTGGCATCAAATTCTTTCCTGTTATCTGCGGCACTGCGTTTAAAAATAAGGG
CGTGCAGAATTTGCTGGACGCGGTTGTTGATTATATGCCGTCCCCACTGGATATTCCTGCTATCAAAGGTCTTGATGCCG
ATACAGGTGAAGAAGTTGAGCGTCCTGCATCTGATGATGTGCCCTTTTCGGCCCTCGGTTTTAAGATTATGACTGACCCT
TTCGTAGGTCAGCTCACTTTTTTCCGCGTGTACTCAGGTGTCATAAATTCTGGTTCGTATGTCTATAATTCCACGAAGGG
CAAAAAAGAACGCATAGGTCGCATTCTGAAGATGCATGCAAACAAGCGCGAAGAGATCAAGGAAGTCTTTGCTGGAGATA
TTGCGGCCGCCGTTGGTCTTAAGTATACGACAACCGGTGATACGCTCTGCACCGAGGATGATGCAGTAATTCTAGAGTCA
ATTGAGTTCCCTGAGCCGGTAATTTCCATCGCGATTGAGCCGAAGACCAAGGCTGACCAGGAAAAGCTCGGTCTGAGTCT
TCAGAAGCTTGCAAGCGAGGATCCGTCCTTCCGTGTGAAAACCGACGAGGAAACCGGACAGACTATTATTTCCGGCATGG
GCGAACTGCATCTCGAAATTATCGTCGACCGACTCCTGCGAGAGTTCAAGGTCGAGGCGAACGTTGGCAAGCCGCAGGTT
GCCTACCGTGAAACGATTACCAAGAAAGTCAAGGTAGAGGGTAAATTCGTCCGTCAGTCCGGTGGTCGTGGACAGTATGG
CCATGTCTGGCTTGAGGTTGAGCCTCAGGAGGCTGGAAAAGGTTACGAATTTGTTGATGCCATTAAAGGTGGAGTTGTAC
CCCGAGAATATATACCTGCTGTTGATAAAGGAATTAAGGAAGCAACAGAGAACGGTGTTCTGGCTGGCTTCCCAGTTGTG
GACATCAAGGTTACCCTCATCGATGGATCGTACCATGAGGTTGACTCTTCGGAGATGGCGTTCAAGATCGCCGGTTCAAT
GGGTTTCAAAGAAGGCTGCCAGAAAGCTTCCCCAATTATCCTCGAGCCTATAGTATCGGTTGAGGTTGTTGTCCCTGACG
AATATATGGGTGATGTCATCGGTGACCTAAATTCGAAGCGTGGCCGGATCATGGGGATGGATACGAGAGCTGGTGCACAG
ATTGTGAACGCCATGGTTCCTATGGCTTCAATGTTCGGTTATTCCACCGATCTGCGCTCCGCTACCCAGGGACGTGCTAC
ATATTCGATGACGTTTGATCACTACGAGCCGGTACCTAAGTCGGTAGCAGAAGAGATTGTAGCTAAAGCAAAAGGTTAA

Upstream 100 bases:

>100_bases
CGCGGTGAAGAAGCGTGAGGATGTACATAAGATGGCAGAAGCCAACCGCGCTTTTGCCCACTATCGCTGGTAAGTAGTTC
AGATCTGTTTGGAGGATTTT

Downstream 100 bases:

>100_bases
GAATACAAGGAAGAGGAGGGCCTGTTATCATGGCGAAGGCTAAATTTGAGCGCACCAAACCGCACGTAAACATTGGCACG
ATTGGTCACGTCGACCACGG

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MPRLATLDKYRNIGIMAHIDAGKTTTTERILYYTGVSHKIGEVHEGAATMDWMEQEQERGITITSAATTCSWRDHRINII
DTPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSETVWRQADKYGVPRIAFINKMDRVGADFFRGVQMIRDRLKANPL
PIQIPIGKEENFKGVVDLVRMKAVIWEEEALGANYHEEEIPADLLVEAQEYRDKMIEEISSHDDTLMEKYLSGEELTEQE
IMDAIRTCTIGIKFFPVICGTAFKNKGVQNLLDAVVDYMPSPLDIPAIKGLDADTGEEVERPASDDVPFSALGFKIMTDP
FVGQLTFFRVYSGVINSGSYVYNSTKGKKERIGRILKMHANKREEIKEVFAGDIAAAVGLKYTTTGDTLCTEDDAVILES
IEFPEPVISIAIEPKTKADQEKLGLSLQKLASEDPSFRVKTDEETGQTIISGMGELHLEIIVDRLLREFKVEANVGKPQV
AYRETITKKVKVEGKFVRQSGGRGQYGHVWLEVEPQEAGKGYEFVDAIKGGVVPREYIPAVDKGIKEATENGVLAGFPVV
DIKVTLIDGSYHEVDSSEMAFKIAGSMGFKEGCQKASPIILEPIVSVEVVVPDEYMGDVIGDLNSKRGRIMGMDTRAGAQ
IVNAMVPMASMFGYSTDLRSATQGRATYSMTFDHYEPVPKSVAEEIVAKAKG

Sequences:

>Translated_692_residues
MPRLATLDKYRNIGIMAHIDAGKTTTTERILYYTGVSHKIGEVHEGAATMDWMEQEQERGITITSAATTCSWRDHRINII
DTPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSETVWRQADKYGVPRIAFINKMDRVGADFFRGVQMIRDRLKANPL
PIQIPIGKEENFKGVVDLVRMKAVIWEEEALGANYHEEEIPADLLVEAQEYRDKMIEEISSHDDTLMEKYLSGEELTEQE
IMDAIRTCTIGIKFFPVICGTAFKNKGVQNLLDAVVDYMPSPLDIPAIKGLDADTGEEVERPASDDVPFSALGFKIMTDP
FVGQLTFFRVYSGVINSGSYVYNSTKGKKERIGRILKMHANKREEIKEVFAGDIAAAVGLKYTTTGDTLCTEDDAVILES
IEFPEPVISIAIEPKTKADQEKLGLSLQKLASEDPSFRVKTDEETGQTIISGMGELHLEIIVDRLLREFKVEANVGKPQV
AYRETITKKVKVEGKFVRQSGGRGQYGHVWLEVEPQEAGKGYEFVDAIKGGVVPREYIPAVDKGIKEATENGVLAGFPVV
DIKVTLIDGSYHEVDSSEMAFKIAGSMGFKEGCQKASPIILEPIVSVEVVVPDEYMGDVIGDLNSKRGRIMGMDTRAGAQ
IVNAMVPMASMFGYSTDLRSATQGRATYSMTFDHYEPVPKSVAEEIVAKAKG
>Mature_691_residues
PRLATLDKYRNIGIMAHIDAGKTTTTERILYYTGVSHKIGEVHEGAATMDWMEQEQERGITITSAATTCSWRDHRINIID
TPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSETVWRQADKYGVPRIAFINKMDRVGADFFRGVQMIRDRLKANPLP
IQIPIGKEENFKGVVDLVRMKAVIWEEEALGANYHEEEIPADLLVEAQEYRDKMIEEISSHDDTLMEKYLSGEELTEQEI
MDAIRTCTIGIKFFPVICGTAFKNKGVQNLLDAVVDYMPSPLDIPAIKGLDADTGEEVERPASDDVPFSALGFKIMTDPF
VGQLTFFRVYSGVINSGSYVYNSTKGKKERIGRILKMHANKREEIKEVFAGDIAAAVGLKYTTTGDTLCTEDDAVILESI
EFPEPVISIAIEPKTKADQEKLGLSLQKLASEDPSFRVKTDEETGQTIISGMGELHLEIIVDRLLREFKVEANVGKPQVA
YRETITKKVKVEGKFVRQSGGRGQYGHVWLEVEPQEAGKGYEFVDAIKGGVVPREYIPAVDKGIKEATENGVLAGFPVVD
IKVTLIDGSYHEVDSSEMAFKIAGSMGFKEGCQKASPIILEPIVSVEVVVPDEYMGDVIGDLNSKRGRIMGMDTRAGAQI
VNAMVPMASMFGYSTDLRSATQGRATYSMTFDHYEPVPKSVAEEIVAKAKG

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=694, Percent_Identity=45.1008645533141, Blast_Score=572, Evalue=1e-163,
Organism=Homo sapiens, GI19923640, Length=709, Percent_Identity=40.9026798307475, Blast_Score=505, Evalue=1e-143,
Organism=Homo sapiens, GI25306283, Length=456, Percent_Identity=44.078947368421, Blast_Score=347, Evalue=3e-95,
Organism=Homo sapiens, GI25306287, Length=286, Percent_Identity=52.0979020979021, Blast_Score=286, Evalue=5e-77,
Organism=Homo sapiens, GI4503483, Length=414, Percent_Identity=27.536231884058, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI94966754, Length=136, Percent_Identity=39.7058823529412, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI217272892, Length=544, Percent_Identity=24.2647058823529, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI217272894, Length=545, Percent_Identity=24.2201834862385, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI157426893, Length=141, Percent_Identity=34.7517730496454, Blast_Score=90, Evalue=9e-18,
Organism=Homo sapiens, GI310132016, Length=117, Percent_Identity=39.3162393162393, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI310110807, Length=117, Percent_Identity=39.3162393162393, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI310123363, Length=117, Percent_Identity=39.3162393162393, Blast_Score=80, Evalue=7e-15,
Organism=Escherichia coli, GI1789738, Length=704, Percent_Identity=64.9147727272727, Blast_Score=927, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=491, Percent_Identity=28.3095723014257, Blast_Score=158, Evalue=9e-40,
Organism=Escherichia coli, GI48994988, Length=136, Percent_Identity=44.1176470588235, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1788922, Length=148, Percent_Identity=37.1621621621622, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=702, Percent_Identity=41.7378917378917, Blast_Score=543, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17556745, Length=726, Percent_Identity=28.6501377410468, Blast_Score=316, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI17506493, Length=414, Percent_Identity=27.7777777777778, Blast_Score=121, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=155, Percent_Identity=38.0645161290323, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71988819, Length=136, Percent_Identity=35.2941176470588, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71988811, Length=136, Percent_Identity=35.2941176470588, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=695, Percent_Identity=42.7338129496403, Blast_Score=557, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6322359, Length=797, Percent_Identity=32.2459222082811, Blast_Score=385, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324707, Length=510, Percent_Identity=26.8627450980392, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6320593, Length=510, Percent_Identity=26.8627450980392, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6323320, Length=175, Percent_Identity=33.7142857142857, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=146, Percent_Identity=36.3013698630137, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24582462, Length=697, Percent_Identity=45.3371592539455, Blast_Score=590, Evalue=1e-168,
Organism=Drosophila melanogaster, GI221458488, Length=723, Percent_Identity=34.9930843706777, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24585709, Length=415, Percent_Identity=26.9879518072289, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24585711, Length=415, Percent_Identity=26.9879518072289, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24585713, Length=415, Percent_Identity=26.9879518072289, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI78706572, Length=137, Percent_Identity=36.4963503649635, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28574573, Length=142, Percent_Identity=36.6197183098592, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 76468; Mature: 76337

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLATLDKYRNIGIMAHIDAGKTTTTERILYYTGVSHKIGEVHEGAATMDWMEQEQERG
CCCCCCHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHCC
ITITSAATTCSWRDHRINIIDTPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSETVW
EEEEECCCEECCCCCEEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHH
RQADKYGVPRIAFINKMDRVGADFFRGVQMIRDRLKANPLPIQIPIGKEENFKGVVDLVR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHH
MKAVIWEEEALGANYHEEEIPADLLVEAQEYRDKMIEEISSHDDTLMEKYLSGEELTEQE
HHHHHCCHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHH
IMDAIRTCTIGIKFFPVICGTAFKNKGVQNLLDAVVDYMPSPLDIPAIKGLDADTGEEVE
HHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHC
RPASDDVPFSALGFKIMTDPFVGQLTFFRVYSGVINSGSYVYNSTKGKKERIGRILKMHA
CCCCCCCCHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHC
NKREEIKEVFAGDIAAAVGLKYTTTGDTLCTEDDAVILESIEFPEPVISIAIEPKTKADQ
CCHHHHHHHHHHHHHHHHCCEEECCCCCEECCCCCCEEECCCCCCCEEEEEECCCCCCCH
EKLGLSLQKLASEDPSFRVKTDEETGQTIISGMGELHLEIIVDRLLREFKVEANVGKPQV
HHHCCHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AYRETITKKVKVEGKFVRQSGGRGQYGHVWLEVEPQEAGKGYEFVDAIKGGVVPREYIPA
HHHHHHHHHEEECEEEEECCCCCCCCCEEEEEECHHHCCCCCHHHHHHHCCCCCHHHHHH
VDKGIKEATENGVLAGFPVVDIKVTLIDGSYHEVDSSEMAFKIAGSMGFKEGCQKASPII
HHHHHHHHHCCCCEECCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHCCCEE
LEPIVSVEVVVPDEYMGDVIGDLNSKRGRIMGMDTRAGAQIVNAMVPMASMFGYSTDLRS
EECCCEEEEEECHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCHHHHH
ATQGRATYSMTFDHYEPVPKSVAEEIVAKAKG
HHCCCEEEEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRLATLDKYRNIGIMAHIDAGKTTTTERILYYTGVSHKIGEVHEGAATMDWMEQEQERG
CCCCCHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHCCCHHHHHHHHHHHCC
ITITSAATTCSWRDHRINIIDTPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEPQSETVW
EEEEECCCEECCCCCEEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHH
RQADKYGVPRIAFINKMDRVGADFFRGVQMIRDRLKANPLPIQIPIGKEENFKGVVDLVR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHH
MKAVIWEEEALGANYHEEEIPADLLVEAQEYRDKMIEEISSHDDTLMEKYLSGEELTEQE
HHHHHCCHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHH
IMDAIRTCTIGIKFFPVICGTAFKNKGVQNLLDAVVDYMPSPLDIPAIKGLDADTGEEVE
HHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHC
RPASDDVPFSALGFKIMTDPFVGQLTFFRVYSGVINSGSYVYNSTKGKKERIGRILKMHA
CCCCCCCCHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHC
NKREEIKEVFAGDIAAAVGLKYTTTGDTLCTEDDAVILESIEFPEPVISIAIEPKTKADQ
CCHHHHHHHHHHHHHHHHCCEEECCCCCEECCCCCCEEECCCCCCCEEEEEECCCCCCCH
EKLGLSLQKLASEDPSFRVKTDEETGQTIISGMGELHLEIIVDRLLREFKVEANVGKPQV
HHHCCHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AYRETITKKVKVEGKFVRQSGGRGQYGHVWLEVEPQEAGKGYEFVDAIKGGVVPREYIPA
HHHHHHHHHEEECEEEEECCCCCCCCCEEEEEECHHHCCCCCHHHHHHHCCCCCHHHHHH
VDKGIKEATENGVLAGFPVVDIKVTLIDGSYHEVDSSEMAFKIAGSMGFKEGCQKASPII
HHHHHHHHHCCCCEECCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHCCCEE
LEPIVSVEVVVPDEYMGDVIGDLNSKRGRIMGMDTRAGAQIVNAMVPMASMFGYSTDLRS
EECCCEEEEEECHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCHHHHH
ATQGRATYSMTFDHYEPVPKSVAEEIVAKAKG
HHCCCEEEEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA