Click here to switch to the map view.

The map label for this gene is trpS2 [H]

Identifier: 118579018

GI number: 118579018

Start: 617746

End: 618813

Strand: Reverse

Name: trpS2 [H]

Synonym: Ppro_0579

Alternate gene names: 118579018

Gene position: 618813-617746 (Counterclockwise)

Preceding gene: 118579019

Following gene: 118579017

Centisome position: 15.44

GC content: 59.93

Gene sequence:

>1068_bases
ATGCATGAAGGATCATTCGAGGCCACACTGAAACGAAGCAAACAACTGGCGGCGGAGCTTGAGCAGACCCCCAAGAAATT
CCGTGTCCTGACCGGCGACCGGCCGACCGGGCGGCTGCATGTCGGCCACCTGTTCGGTTCGCTCCAGAACCGGATCAGAC
TCCAGTCCCTGGGCGTGCCGACCTTCATCGTGATAGCGGACTACCAGGTCCTGACGGATCGGGACCACTTCGACTCCATC
GCGGAGAACACGCTGCAACTGGCGATCGACTACCTTTCCGTGGGTATCGACCCTATCGGGGAAGACACCGACATCTTTCC
CCACAGTCACGTGCCGGAACTCAACCAACTCCTGCTCCCCTTTCTGACCCTGGTTACCCTGTCGGAACTGGACCGCAATC
CGACGGTCAAGGAAGAGATCCAGTCTGCCGGACTCAAGCGCATCAACGCGGGTATGTACACCTATCCGATTCACCAGGCC
GCGGACATACTGTTTTGCAAGGGAAACGTGGTTCCGGTGGGAAAAGACCAGCTGCCGCACCTGGAAATCACCCGCACCAT
CGCCCGCCGGTTCAATGAAAAGTTCGGTCAGGGGAAGCAGGTGTTTCCGGAGCCGCAACCGTTGCTCAGCCAGGCGCCCC
TGATCCTGGGGCTGGACGGTTCCCAGAAGATGAGCAAAAGCCGCAACAATGCCATCATGCTGAGCGCGACCGCCGAGGAG
ACCGCCGCGCTGATCAAAAAAGCCAAGACCGACTCGGAGCGGCGCATCAGCTACGATCCTGTGAACCGCCCCGAGGTTTC
TAACCTGATTCTCCTGGCCTCCCTCTCCACCGACCGGGATCCGGAGAGCATTGCCGAAGGCATCGGCGATGGCGGGTCGG
GCAAACTGAAATTATTCGTGACCGAGGCGCTCAACGACTATCTCCGTCCGATCCGGGAGAAGCGACGGGAGCTGGAAACC
GATCTGGGCTTCGTGCGTGGCGTGCTGCGAAAGGGGATAGAGGGAGCCCGGGCAGTGGCCATCGAGACCCTGGACGAAGT
GAGAAGCGTGATGAACATGAACATCTAG

Upstream 100 bases:

>100_bases
AAAAACCGGATCCGCGCAACCGCAACACGCCATCTGCGCGAGCCCGGCCTTCCAGCCATTAGGTGAAATCATCAGATAAG
AACAGGGGATTATGAGCGAC

Downstream 100 bases:

>100_bases
CCGGGCCATGCCAACCCTTCTGGTCGAAATCCGCTCTCGCGCCATTGACGTAGTTTGTATGGAGCTTGCATGAGATATAT
TATAGTTTCGCCTGCATACG

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase II; TrpRS II; Tryptophanyl-tRNA synthetase II [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MHEGSFEATLKRSKQLAAELEQTPKKFRVLTGDRPTGRLHVGHLFGSLQNRIRLQSLGVPTFIVIADYQVLTDRDHFDSI
AENTLQLAIDYLSVGIDPIGEDTDIFPHSHVPELNQLLLPFLTLVTLSELDRNPTVKEEIQSAGLKRINAGMYTYPIHQA
ADILFCKGNVVPVGKDQLPHLEITRTIARRFNEKFGQGKQVFPEPQPLLSQAPLILGLDGSQKMSKSRNNAIMLSATAEE
TAALIKKAKTDSERRISYDPVNRPEVSNLILLASLSTDRDPESIAEGIGDGGSGKLKLFVTEALNDYLRPIREKRRELET
DLGFVRGVLRKGIEGARAVAIETLDEVRSVMNMNI

Sequences:

>Translated_355_residues
MHEGSFEATLKRSKQLAAELEQTPKKFRVLTGDRPTGRLHVGHLFGSLQNRIRLQSLGVPTFIVIADYQVLTDRDHFDSI
AENTLQLAIDYLSVGIDPIGEDTDIFPHSHVPELNQLLLPFLTLVTLSELDRNPTVKEEIQSAGLKRINAGMYTYPIHQA
ADILFCKGNVVPVGKDQLPHLEITRTIARRFNEKFGQGKQVFPEPQPLLSQAPLILGLDGSQKMSKSRNNAIMLSATAEE
TAALIKKAKTDSERRISYDPVNRPEVSNLILLASLSTDRDPESIAEGIGDGGSGKLKLFVTEALNDYLRPIREKRRELET
DLGFVRGVLRKGIEGARAVAIETLDEVRSVMNMNI
>Mature_355_residues
MHEGSFEATLKRSKQLAAELEQTPKKFRVLTGDRPTGRLHVGHLFGSLQNRIRLQSLGVPTFIVIADYQVLTDRDHFDSI
AENTLQLAIDYLSVGIDPIGEDTDIFPHSHVPELNQLLLPFLTLVTLSELDRNPTVKEEIQSAGLKRINAGMYTYPIHQA
ADILFCKGNVVPVGKDQLPHLEITRTIARRFNEKFGQGKQVFPEPQPLLSQAPLILGLDGSQKMSKSRNNAIMLSATAEE
TAALIKKAKTDSERRISYDPVNRPEVSNLILLASLSTDRDPESIAEGIGDGGSGKLKLFVTEALNDYLRPIREKRRELET
DLGFVRGVLRKGIEGARAVAIETLDEVRSVMNMNI

Specific function: Catalyzes the formation of 5'adenyl-Trp and tRNA(Trp) but with 5-fold less activity than trpRS. Increases the solubility of the nitric oxide synthase oxygenase (nos), as well as its affinity for substrate L-arginine and its nitric-oxide synthase activity.

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=359, Percent_Identity=29.5264623955432, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI41352700, Length=218, Percent_Identity=32.1100917431193, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI1789786, Length=326, Percent_Identity=34.3558282208589, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71982800, Length=331, Percent_Identity=31.4199395770393, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71982793, Length=333, Percent_Identity=31.2312312312312, Blast_Score=131, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6320474, Length=348, Percent_Identity=29.0229885057471, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24666151, Length=332, Percent_Identity=31.9277108433735, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 39438; Mature: 39438

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEGSFEATLKRSKQLAAELEQTPKKFRVLTGDRPTGRLHVGHLFGSLQNRIRLQSLGVP
CCCCCHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCEEHHHHHHHHHHHHHHHHCCCC
TFIVIADYQVLTDRDHFDSIAENTLQLAIDYLSVGIDPIGEDTDIFPHSHVPELNQLLLP
EEEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
FLTLVTLSELDRNPTVKEEIQSAGLKRINAGMYTYPIHQAADILFCKGNVVPVGKDQLPH
HHHHHHHHHHCCCCCHHHHHHHCCHHHHCCCCEEECHHHCCCEEEECCCEEECCCCCCCC
LEITRTIARRFNEKFGQGKQVFPEPQPLLSQAPLILGLDGSQKMSKSRNNAIMLSATAEE
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEECCCCHHHHHCCCCEEEEEECHHH
TAALIKKAKTDSERRISYDPVNRPEVSNLILLASLSTDRDPESIAEGIGDGGSGKLKLFV
HHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCEEEEEE
TEALNDYLRPIREKRRELETDLGFVRGVLRKGIEGARAVAIETLDEVRSVMNMNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHEGSFEATLKRSKQLAAELEQTPKKFRVLTGDRPTGRLHVGHLFGSLQNRIRLQSLGVP
CCCCCHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCEEHHHHHHHHHHHHHHHHCCCC
TFIVIADYQVLTDRDHFDSIAENTLQLAIDYLSVGIDPIGEDTDIFPHSHVPELNQLLLP
EEEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
FLTLVTLSELDRNPTVKEEIQSAGLKRINAGMYTYPIHQAADILFCKGNVVPVGKDQLPH
HHHHHHHHHHCCCCCHHHHHHHCCHHHHCCCCEEECHHHCCCEEEECCCEEECCCCCCCC
LEITRTIARRFNEKFGQGKQVFPEPQPLLSQAPLILGLDGSQKMSKSRNNAIMLSATAEE
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEECCCCHHHHHCCCCEEEEEECHHH
TAALIKKAKTDSERRISYDPVNRPEVSNLILLASLSTDRDPESIAEGIGDGGSGKLKLFV
HHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCEEEEEE
TEALNDYLRPIREKRRELETDLGFVRGVLRKGIEGARAVAIETLDEVRSVMNMNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]