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The map label for this gene is def [H]

Identifier: 118578953

GI number: 118578953

Start: 540502

End: 541020

Strand: Reverse

Name: def [H]

Synonym: Ppro_0514

Alternate gene names: 118578953

Gene position: 541020-540502 (Counterclockwise)

Preceding gene: 118578954

Following gene: 118578952

Centisome position: 13.5

GC content: 62.24

Gene sequence:

>519_bases
ATGATTCGCACCATTCTCACCTACCCAAATCCCGAGCTGAAAAAGAAATCGGCAGCGGTTACCATCATAACCGACGAGAT
CCGCGAACTGGTCAGTGATATGACCGAAACCATGTACCACGCCCCCGGCGTGGGGCTGGCAGCCCCCCAGATCGGCGTCC
ATCAGCGGGTGATCATCATCGATGTCTCCGCCCACGACGAGCCGAACGAGCTGATTATAGCAATCAACCCGGTCATCGTC
CATGCCGAGGGAGAGAGCTACGAGGAGGAGGGCTGCCTGTCGGTACCCAAATACGCCGCCAACGTGCGCCGCCACGCCAG
CGTGACGGTCAGGGGCCTGGACCTGGAGGGGAGGGAACAGACCTGGCATGCCGAGGGGCTCTTGGCCATCGCCTTCCAGC
ACGAGATCGATCACCTGGACGGCAGACTCTTCGTCGACCACCTCTCGCCCCTCAAGCGCGATCTGTTCCAGCGCAGGGCC
CGCAAGGCGGCGGAAGCGAAAGCAGCGGGGGAGCGATGA

Upstream 100 bases:

>100_bases
GTCATGACCGCCTCCTCCGCCATCCCCGAAAAGATGAAAATTGATTTTTAACCGTTGAAACTATATACTGCTTCGACAAA
ATTCCGTCGCAAGGATGTCT

Downstream 100 bases:

>100_bases
GCAGCAGGAGCATCGTCTTCATGGGAACGCCCGAGTTTGCCTGCCCCACCCTGCAGGCCCTGATCGACCGCGGAGAACGG
CTGCTGGCAGTGGTCACCCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MIRTILTYPNPELKKKSAAVTIITDEIRELVSDMTETMYHAPGVGLAAPQIGVHQRVIIIDVSAHDEPNELIIAINPVIV
HAEGESYEEEGCLSVPKYAANVRRHASVTVRGLDLEGREQTWHAEGLLAIAFQHEIDHLDGRLFVDHLSPLKRDLFQRRA
RKAAEAKAAGER

Sequences:

>Translated_172_residues
MIRTILTYPNPELKKKSAAVTIITDEIRELVSDMTETMYHAPGVGLAAPQIGVHQRVIIIDVSAHDEPNELIIAINPVIV
HAEGESYEEEGCLSVPKYAANVRRHASVTVRGLDLEGREQTWHAEGLLAIAFQHEIDHLDGRLFVDHLSPLKRDLFQRRA
RKAAEAKAAGER
>Mature_172_residues
MIRTILTYPNPELKKKSAAVTIITDEIRELVSDMTETMYHAPGVGLAAPQIGVHQRVIIIDVSAHDEPNELIIAINPVIV
HAEGESYEEEGCLSVPKYAANVRRHASVTVRGLDLEGREQTWHAEGLLAIAFQHEIDHLDGRLFVDHLSPLKRDLFQRRA
RKAAEAKAAGER

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=30.5732484076433, Blast_Score=74, Evalue=8e-14,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=44.5121951219512, Blast_Score=142, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24645726, Length=178, Percent_Identity=31.4606741573034, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24645728, Length=178, Percent_Identity=27.5280898876405, Blast_Score=73, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19156; Mature: 19156

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTILTYPNPELKKKSAAVTIITDEIRELVSDMTETMYHAPGVGLAAPQIGVHQRVIII
CCEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
DVSAHDEPNELIIAINPVIVHAEGESYEEEGCLSVPKYAANVRRHASVTVRGLDLEGREQ
EECCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCCHHHHHHHHCCEEEEEECCCCCCCC
TWHAEGLLAIAFQHEIDHLDGRLFVDHLSPLKRDLFQRRARKAAEAKAAGER
EECCCCEEEEEEEHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIRTILTYPNPELKKKSAAVTIITDEIRELVSDMTETMYHAPGVGLAAPQIGVHQRVIII
CCEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
DVSAHDEPNELIIAINPVIVHAEGESYEEEGCLSVPKYAANVRRHASVTVRGLDLEGREQ
EECCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCCHHHHHHHHCCEEEEEECCCCCCCC
TWHAEGLLAIAFQHEIDHLDGRLFVDHLSPLKRDLFQRRARKAAEAKAAGER
EECCCCEEEEEEEHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA