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The map label for this gene is oprF [H]

Identifier: 118578882

GI number: 118578882

Start: 462505

End: 463758

Strand: Reverse

Name: oprF [H]

Synonym: Ppro_0441

Alternate gene names: 118578882

Gene position: 463758-462505 (Counterclockwise)

Preceding gene: 118578884

Following gene: 118578871

Centisome position: 11.57

GC content: 60.29

Gene sequence:

>1254_bases
ATGAAAAAGACAGCAAGAATCTCGTTGCTGGTGGCAGGCTCCCTGATAGCTCTGGCAACAGCCGCATCCGCCGAAAATAG
AGAAGGCGCATTCTCGCTCTCACCCGTAATCGGTGGAATCACGTTCGGAGGCGGTCAGCACCTGGAGACCGCGCCGGTCT
ACGGCATCCGTGCCGGGTACAACTTCACCAAGGCGTTGGGCATCGAGGCCCTGTTCGACTACTCCAACACCGAGCCGACC
AGTAACGGCAGCAAGCTCACCAATGGCAAAAACACCGACTACTACCGCATGGGCGGCGAGCTGCTCTACCACTTCTTCCC
CGACAACAAATTCGTCCCCTACTTCGCCGCCGGCTATGCCGCCCATACCTTCAAGGGGGCCGTTCCGGCCGGAGAGAGCT
CAGATGTCAAAGGCGTGGGCGACTACGGCTTGGGCGCCAAGTATTTCCTGAACGACAAGGTCGCCCTGCGCGGCGATGTG
CGCCACCTGATCTATCGCCAGGCAAGCGAGACCCAGCACGCGGTTGAATACACCATGGGCCTCCACTTTCCCTTTGGCGG
AAAGACGGAAGCCGCTCCCGTGGCTGAACCGGCGCCCGAACCGGTGGTGGCACCGGTTGCCCCATCTGACAGCGATGGCG
ACGGCGTGTTCGATACCGCTGACAACTGTCCCGGAACTCCCCAGGGCGTGAAGGTCGACGCAAAGGGCTGCCCCCTGGAT
TCCGACAGGGACGGCGTCTATGACTATCAGGATAACTGCCCCGGAACGCCGGAAGGCGTGAAGGTCGACACCAAAGGTTG
TCCGCTGGATTCCGACAAAGACGGCGTCTATGACTCCTATGACAAGTGCCCCGGCACCCCGGAAGGCACCACCGTGGACA
AGACCGGTTGCCCGGTCAAGCTCTGCAGCCCCACGGTTCTCAACATCGAATTCGACACCAACAAGTCCAACATCAAGCCA
CAGTACAATGACGAGCTGAAAAGGGTTGCCGACTTCCTGGCCGAATTCCCCACCGCCAAGGGAGCCATCGAAGGCCACAC
CGACAACGTGGGCGGCAAGGCATACAACATGAAGCTCTCCCAGCGCCGCGCCAACAGCGTACGCAGCTATCTCATCAAGA
CCTTCAATGTCGCTCCGGAACGCATCAGCGCCAAGGGCCATGGCCTCACCAAACCGGTAGCCAGCAACAAGACCGCCGAC
GGCAGGAAGCAGAACCGCCGCATCGAGGCAAACTTCACCTGCAACGGCAAATAA

Upstream 100 bases:

>100_bases
CCGGGGACTTTTGTATTGCATGAAGACAAAAGAGTGTGCTAAGAATTTTAAGATGGAGATTGCTTAATCGTAGTACAAAC
CCAAAAGAAGGTTCAACAAC

Downstream 100 bases:

>100_bases
CGGCCGGCTGAAGCATTCCTCTCGCACAAACGCCCCTCCGGAATCGATCCGGAGGGGCGTTTGTGCATTTCACCGGGCCA
GGAAGATTCTATTCTTCGTT

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MKKTARISLLVAGSLIALATAASAENREGAFSLSPVIGGITFGGGQHLETAPVYGIRAGYNFTKALGIEALFDYSNTEPT
SNGSKLTNGKNTDYYRMGGELLYHFFPDNKFVPYFAAGYAAHTFKGAVPAGESSDVKGVGDYGLGAKYFLNDKVALRGDV
RHLIYRQASETQHAVEYTMGLHFPFGGKTEAAPVAEPAPEPVVAPVAPSDSDGDGVFDTADNCPGTPQGVKVDAKGCPLD
SDRDGVYDYQDNCPGTPEGVKVDTKGCPLDSDKDGVYDSYDKCPGTPEGTTVDKTGCPVKLCSPTVLNIEFDTNKSNIKP
QYNDELKRVADFLAEFPTAKGAIEGHTDNVGGKAYNMKLSQRRANSVRSYLIKTFNVAPERISAKGHGLTKPVASNKTAD
GRKQNRRIEANFTCNGK

Sequences:

>Translated_417_residues
MKKTARISLLVAGSLIALATAASAENREGAFSLSPVIGGITFGGGQHLETAPVYGIRAGYNFTKALGIEALFDYSNTEPT
SNGSKLTNGKNTDYYRMGGELLYHFFPDNKFVPYFAAGYAAHTFKGAVPAGESSDVKGVGDYGLGAKYFLNDKVALRGDV
RHLIYRQASETQHAVEYTMGLHFPFGGKTEAAPVAEPAPEPVVAPVAPSDSDGDGVFDTADNCPGTPQGVKVDAKGCPLD
SDRDGVYDYQDNCPGTPEGVKVDTKGCPLDSDKDGVYDSYDKCPGTPEGTTVDKTGCPVKLCSPTVLNIEFDTNKSNIKP
QYNDELKRVADFLAEFPTAKGAIEGHTDNVGGKAYNMKLSQRRANSVRSYLIKTFNVAPERISAKGHGLTKPVASNKTAD
GRKQNRRIEANFTCNGK
>Mature_417_residues
MKKTARISLLVAGSLIALATAASAENREGAFSLSPVIGGITFGGGQHLETAPVYGIRAGYNFTKALGIEALFDYSNTEPT
SNGSKLTNGKNTDYYRMGGELLYHFFPDNKFVPYFAAGYAAHTFKGAVPAGESSDVKGVGDYGLGAKYFLNDKVALRGDV
RHLIYRQASETQHAVEYTMGLHFPFGGKTEAAPVAEPAPEPVVAPVAPSDSDGDGVFDTADNCPGTPQGVKVDAKGCPLD
SDRDGVYDYQDNCPGTPEGVKVDTKGCPLDSDKDGVYDSYDKCPGTPEGTTVDKTGCPVKLCSPTVLNIEFDTNKSNIKP
QYNDELKRVADFLAEFPTAKGAIEGHTDNVGGKAYNMKLSQRRANSVRSYLIKTFNVAPERISAKGHGLTKPVASNKTAD
GRKQNRRIEANFTCNGK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=133, Percent_Identity=41.3533834586466, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788957, Length=105, Percent_Identity=34.2857142857143, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 44528; Mature: 44528

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTARISLLVAGSLIALATAASAENREGAFSLSPVIGGITFGGGQHLETAPVYGIRAGY
CCCCEEEEEEECCHHHHHHHHCCCCCCCCCEEECHHCCEEEECCCCCCCCCCCEEEECCC
NFTKALGIEALFDYSNTEPTSNGSKLTNGKNTDYYRMGGELLYHFFPDNKFVPYFAAGYA
CHHHHHCHHHEECCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEECCCCCCCCHHHCCHH
AHTFKGAVPAGESSDVKGVGDYGLGAKYFLNDKVALRGDVRHLIYRQASETQHAVEYTMG
HHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEHHHHHHHHHHHHCHHHHHEEEEEE
LHFPFGGKTEAAPVAEPAPEPVVAPVAPSDSDGDGVFDTADNCPGTPQGVKVDAKGCPLD
EECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEECCCCCCCCCCCEEECCCCCCCC
SDRDGVYDYQDNCPGTPEGVKVDTKGCPLDSDKDGVYDSYDKCPGTPEGTTVDKTGCPVK
CCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHCCCCCCCCCEECCCCCCEE
LCSPTVLNIEFDTNKSNIKPQYNDELKRVADFLAEFPTAKGAIEGHTDNVGGKAYNMKLS
ECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHH
QRRANSVRSYLIKTFNVAPERISAKGHGLTKPVASNKTADGRKQNRRIEANFTCNGK
HHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCHHHCCEEEEEEEECCC
>Mature Secondary Structure
MKKTARISLLVAGSLIALATAASAENREGAFSLSPVIGGITFGGGQHLETAPVYGIRAGY
CCCCEEEEEEECCHHHHHHHHCCCCCCCCCEEECHHCCEEEECCCCCCCCCCCEEEECCC
NFTKALGIEALFDYSNTEPTSNGSKLTNGKNTDYYRMGGELLYHFFPDNKFVPYFAAGYA
CHHHHHCHHHEECCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEECCCCCCCCHHHCCHH
AHTFKGAVPAGESSDVKGVGDYGLGAKYFLNDKVALRGDVRHLIYRQASETQHAVEYTMG
HHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEHHHHHHHHHHHHCHHHHHEEEEEE
LHFPFGGKTEAAPVAEPAPEPVVAPVAPSDSDGDGVFDTADNCPGTPQGVKVDAKGCPLD
EECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEECCCCCCCCCCCEEECCCCCCCC
SDRDGVYDYQDNCPGTPEGVKVDTKGCPLDSDKDGVYDSYDKCPGTPEGTTVDKTGCPVK
CCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHCCCCCCCCCEECCCCCCEE
LCSPTVLNIEFDTNKSNIKPQYNDELKRVADFLAEFPTAKGAIEGHTDNVGGKAYNMKLS
ECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHH
QRRANSVRSYLIKTFNVAPERISAKGHGLTKPVASNKTADGRKQNRRIEANFTCNGK
HHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCHHHCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]