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The map label for this gene is mexA [H]

Identifier: 118578773

GI number: 118578773

Start: 332391

End: 333575

Strand: Reverse

Name: mexA [H]

Synonym: Ppro_0331

Alternate gene names: 118578773

Gene position: 333575-332391 (Counterclockwise)

Preceding gene: 118578774

Following gene: 118578772

Centisome position: 8.32

GC content: 65.15

Gene sequence:

>1185_bases
ATGCATGTCACACAAACAATCAAACGCGCCACTCTCCTGGCCGGTATTGCCGGGGTACTCATGCTCAACGGCTGCGGCAA
TAAACAGTCGGGCGCAACCCCACCCCCCGCTCCCCCCGTGGTAGGCGTCGTCATCATCAAACCCCAGCGGGTGGAGTTGA
CAACGGAGCTGGCCGGACGCACGGCCGCCTACCTGGTGGCCGAAGTGCGTCCCCAGGTGAACGGCATCGTCCAGAAGCGG
CTGTTCACCGAAGGGAGCGACGTCAAGGCCGGCCAGGTCCTCTACCAGATCGATCCCTCCACCTATCGCGCCGCCCATGC
CAGCGCCAGGGCCTCGCTCTCCCGCGCCGAGGCGACGCTGGTCTCGGCCCGGCTCAAAGCCAAGCGCTACGGGGAACTGG
TCAAGATCAACGCCGTCAGCCAGCAGGATCATGACGATGCCTACGCTGCCCTGAAACAGGCCGAGGCGGACGTGGAGTCC
AGCAAGGCAGCCGTGGAATCGGCCCGCATCAACCTGGCCTATACCCGGGTTACCGCCCCCATCTCCGGAAGAATCGGCAG
GTCGCTGATCACCACCGGCGCGCTGATGACCGCCAACCAGGCCACCGCCCTGGCCACCATCCAGCAGTTGAGCCCGGTCT
ACGTGGACATAACCCAGACCAGCGCCGAATTGCTGCGCCTGAAGCACGCCCTGGCCAGCGGCCAGATGAAGGGGGGCGGC
CAGGGAGGCACTAAGGTCAAGCTGCTTCTGGAAGACGGCAGCACCTATCCCCATGAAGGCGTGCTGAAGTTTTCCGACGT
GACCGTCGACCAGAGCACCGGCTCGGTCACCCTGCGCTCCGTCTTTGCCAACCCGAAGCAGCTTTTGCTCCCCGGCATGT
ACGTGCGCGCCGTGATCCAGGAGGGGGTCAACGAAAACGCCATCCTGGCGCCCCAGAAGGGTGTCACCCGCAACTCGGCC
GGAAATCCCACCGCACTGGTGGTGGGAGCCAACAACAAGGTTGAGTCGCGCCTGCTCACGGTTTCCCGAACCATCGGCGA
CTCCTGGCTGGTCAACGAGGGGCTCAAGGCCGGCGACCGACTGATCGTCGAGGGCACCCAGAAGGCCAAGCCGGGGAGCA
GCGTCAAACCGGTGGCATACACTCCACGCACGGGCGCGGCAACCGCAACGGCAGCGGCACACTAG

Upstream 100 bases:

>100_bases
CTATTCGCCCTGCTTGACCTGACCGGGTCTGCAGCACAAACTTCCACACAAAAAGACTGAACGTTCATTCATTTTATTTC
ACGTATCGAGGTAGCGAACC

Downstream 100 bases:

>100_bases
AGGAGATTTCACATGGCTCAGTTTTTCATCGATCGCCCCATCTTCGCCTGGGTGATCGCCATCATCATCATGCTGGCCGG
CATACTGGCCATCAAGCAGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MHVTQTIKRATLLAGIAGVLMLNGCGNKQSGATPPPAPPVVGVVIIKPQRVELTTELAGRTAAYLVAEVRPQVNGIVQKR
LFTEGSDVKAGQVLYQIDPSTYRAAHASARASLSRAEATLVSARLKAKRYGELVKINAVSQQDHDDAYAALKQAEADVES
SKAAVESARINLAYTRVTAPISGRIGRSLITTGALMTANQATALATIQQLSPVYVDITQTSAELLRLKHALASGQMKGGG
QGGTKVKLLLEDGSTYPHEGVLKFSDVTVDQSTGSVTLRSVFANPKQLLLPGMYVRAVIQEGVNENAILAPQKGVTRNSA
GNPTALVVGANNKVESRLLTVSRTIGDSWLVNEGLKAGDRLIVEGTQKAKPGSSVKPVAYTPRTGAATATAAAH

Sequences:

>Translated_394_residues
MHVTQTIKRATLLAGIAGVLMLNGCGNKQSGATPPPAPPVVGVVIIKPQRVELTTELAGRTAAYLVAEVRPQVNGIVQKR
LFTEGSDVKAGQVLYQIDPSTYRAAHASARASLSRAEATLVSARLKAKRYGELVKINAVSQQDHDDAYAALKQAEADVES
SKAAVESARINLAYTRVTAPISGRIGRSLITTGALMTANQATALATIQQLSPVYVDITQTSAELLRLKHALASGQMKGGG
QGGTKVKLLLEDGSTYPHEGVLKFSDVTVDQSTGSVTLRSVFANPKQLLLPGMYVRAVIQEGVNENAILAPQKGVTRNSA
GNPTALVVGANNKVESRLLTVSRTIGDSWLVNEGLKAGDRLIVEGTQKAKPGSSVKPVAYTPRTGAATATAAAH
>Mature_394_residues
MHVTQTIKRATLLAGIAGVLMLNGCGNKQSGATPPPAPPVVGVVIIKPQRVELTTELAGRTAAYLVAEVRPQVNGIVQKR
LFTEGSDVKAGQVLYQIDPSTYRAAHASARASLSRAEATLVSARLKAKRYGELVKINAVSQQDHDDAYAALKQAEADVES
SKAAVESARINLAYTRVTAPISGRIGRSLITTGALMTANQATALATIQQLSPVYVDITQTSAELLRLKHALASGQMKGGG
QGGTKVKLLLEDGSTYPHEGVLKFSDVTVDQSTGSVTLRSVFANPKQLLLPGMYVRAVIQEGVNENAILAPQKGVTRNSA
GNPTALVVGANNKVESRLLTVSRTIGDSWLVNEGLKAGDRLIVEGTQKAKPGSSVKPVAYTPRTGAATATAAAH

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=363, Percent_Identity=55.6473829201102, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1789665, Length=362, Percent_Identity=53.8674033149171, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI1789929, Length=352, Percent_Identity=50.5681818181818, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI87082036, Length=357, Percent_Identity=28.8515406162465, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI87081796, Length=396, Percent_Identity=26.2626262626263, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41284; Mature: 41284

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVTQTIKRATLLAGIAGVLMLNGCGNKQSGATPPPAPPVVGVVIIKPQRVELTTELAGR
CCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEEEECCCCEEEEHHHCCC
TAAYLVAEVRPQVNGIVQKRLFTEGSDVKAGQVLYQIDPSTYRAAHASARASLSRAEATL
HHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
VSARLKAKRYGELVKINAVSQQDHDDAYAALKQAEADVESSKAAVESARINLAYTRVTAP
HHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECC
ISGRIGRSLITTGALMTANQATALATIQQLSPVYVDITQTSAELLRLKHALASGQMKGGG
CCCCCCHHHHHHCCEEECCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCC
QGGTKVKLLLEDGSTYPHEGVLKFSDVTVDQSTGSVTLRSVFANPKQLLLPGMYVRAVIQ
CCCCEEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEHHCCCHHHHCCHHHHHHHHH
EGVNENAILAPQKGVTRNSAGNPTALVVGANNKVESRLLTVSRTIGDSWLVNEGLKAGDR
CCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHEEHHHHCCCCHHHHCCCCCCCE
LIVEGTQKAKPGSSVKPVAYTPRTGAATATAAAH
EEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MHVTQTIKRATLLAGIAGVLMLNGCGNKQSGATPPPAPPVVGVVIIKPQRVELTTELAGR
CCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEEEECCCCEEEEHHHCCC
TAAYLVAEVRPQVNGIVQKRLFTEGSDVKAGQVLYQIDPSTYRAAHASARASLSRAEATL
HHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
VSARLKAKRYGELVKINAVSQQDHDDAYAALKQAEADVESSKAAVESARINLAYTRVTAP
HHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECC
ISGRIGRSLITTGALMTANQATALATIQQLSPVYVDITQTSAELLRLKHALASGQMKGGG
CCCCCCHHHHHHCCEEECCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCC
QGGTKVKLLLEDGSTYPHEGVLKFSDVTVDQSTGSVTLRSVFANPKQLLLPGMYVRAVIQ
CCCCEEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEHHCCCHHHHCCHHHHHHHHH
EGVNENAILAPQKGVTRNSAGNPTALVVGANNKVESRLLTVSRTIGDSWLVNEGLKAGDR
CCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHEEHHHHCCCCHHHHCCCCCCCE
LIVEGTQKAKPGSSVKPVAYTPRTGAATATAAAH
EEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]