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The map label for this gene is oprM [H]

Identifier: 118578771

GI number: 118578771

Start: 327788

End: 329212

Strand: Reverse

Name: oprM [H]

Synonym: Ppro_0329

Alternate gene names: 118578771

Gene position: 329212-327788 (Counterclockwise)

Preceding gene: 118578772

Following gene: 118578769

Centisome position: 8.21

GC content: 63.02

Gene sequence:

>1425_bases
ATGAAACACGCCTTCTTGAAAAAAGCCTTCTTTCTTCACTTGAGCGGGCTGTTATTCCTGGCCGGCTGTTCCATGGCGCC
CAACTATACCCGGCCTGAGGCGCCGGTTCCCCAGGCCTGGCCCAGCGGACCGGCCTACAAGGGCGCTGCCAGCCAAGCAC
TGGAGCCTGAGGCGGTGCAGCTGAAGTGGGAGCAGTTCTTTATCAACCCGCAGCTGCGAAAACTGATCAAACTGGCCCTG
GACAACAACCGCGACCTGCGGATTGCCACTCTCAACGTGGAGAAGTCCCGTGCCCAATACCGCATCCAGCGCGCCGACCT
GCTGCCCAGCATCAATGGTAACGGCAACGCCACCATGGAGCGGACACCGTCCGACCTCTCCAGCAGCGGTGAGGCCATAA
CCTCCCACAGCTATACCCTGAATCTGGGGGTCAGCAGCTACGAAGTGGATCTGTTCGGCCGCGTACAGAGCCTCAAGGAT
CAGGCCCTGGAGCAGTTCCTGTCAACGGAGCAGGCGGTGCGCAGCGTGCGCCTCTCACTGGTGGCCGAGGTGGCCAACAA
CTACCTGACCCTGGCCGCCGATCGCGAGCGACTGAAGTTGGCCAGGGAAACGCTTGAAAGCCAGCGGGTTTCCTACAACC
TGATCAAGAGCCGTTTCTCCGCTGGCGCCTCGTCCGAACTGGACCTGCGCCAGGCCCAGACCAGCGTGGATTCGGCACGG
GTTGACGTGGCCCGCTACACCAGCCAGGTGGCGCAAGACGAGAACCTGCTGGCACTGGTGGTGGGAGGCCCGGTTCCGGC
GGAACTCCTCCCCAGTGGCTTCGAAGCAGCGGGAATGATGCGGGAGCTGTCCAGCGGTGTGCCGTCGGAGGTGCTCCAGC
GGAGACCGGACATCATGGAGGCCGAACACCTGCTCAAGGGGGCCAACGCCAACATCGGCGCGGCCCGGGCAGCCTTCTTC
CCCCGCATCACCCTCAACACCAGCATCGGCACCTCCAGCGACCAGCTGAGCGGTCTCTTTTCGCCCGGCTCATTCGCCTG
GGGTTTCGCCCCCCAATTCACCCTGCCGATCTTCGACTTCGGCAGGAACAAGGCAGGCCTGACCGTTGCCGAGCGGGACC
GGGACATCTACCTGGCCCAGTACGAAAAGTCGATCCAGAGCGCCTTCCGGGAGGTGGCCGACGCCTTGGCCGTAAACGGG
ACAATTACGGAGCAGTTGGATGCGCAGCAGTCCCTGGTCCAGGCCACATCGGAAAGTTACCGTCTCTCCCAGGCCCGCTA
CATGGGGGGTATCAGCAGCTACTTGGACGTGCTGGTTTCCCAGCGCTCGCTCTACACCACGCAGCAGTCGCTGATCGGCG
TCAACCTGACCAAGCTCTCCAACCTGGTGACGCTCTACAAGGTGCTGGGCGGAGGGAGCGAGTAG

Upstream 100 bases:

>100_bases
ATACCGTTCTTCTTTGTCCTCGTATCCCGACTGTTCGGCAGAAAAAACCCGTCAGCCAACGAAAAGAGCGAACGTATCAC
CGCACCGGAGGTCAACTGAC

Downstream 100 bases:

>100_bases
GCCCTCCCCCTGGACGCAGAGACACCGTGATCCCCTTTCCCGGGGAATCACGGCGTCTCGTCATTTCAGCCCACCCTTGC
CGCAGGTATATCAGAGGATC

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MKHAFLKKAFFLHLSGLLFLAGCSMAPNYTRPEAPVPQAWPSGPAYKGAASQALEPEAVQLKWEQFFINPQLRKLIKLAL
DNNRDLRIATLNVEKSRAQYRIQRADLLPSINGNGNATMERTPSDLSSSGEAITSHSYTLNLGVSSYEVDLFGRVQSLKD
QALEQFLSTEQAVRSVRLSLVAEVANNYLTLAADRERLKLARETLESQRVSYNLIKSRFSAGASSELDLRQAQTSVDSAR
VDVARYTSQVAQDENLLALVVGGPVPAELLPSGFEAAGMMRELSSGVPSEVLQRRPDIMEAEHLLKGANANIGAARAAFF
PRITLNTSIGTSSDQLSGLFSPGSFAWGFAPQFTLPIFDFGRNKAGLTVAERDRDIYLAQYEKSIQSAFREVADALAVNG
TITEQLDAQQSLVQATSESYRLSQARYMGGISSYLDVLVSQRSLYTTQQSLIGVNLTKLSNLVTLYKVLGGGSE

Sequences:

>Translated_474_residues
MKHAFLKKAFFLHLSGLLFLAGCSMAPNYTRPEAPVPQAWPSGPAYKGAASQALEPEAVQLKWEQFFINPQLRKLIKLAL
DNNRDLRIATLNVEKSRAQYRIQRADLLPSINGNGNATMERTPSDLSSSGEAITSHSYTLNLGVSSYEVDLFGRVQSLKD
QALEQFLSTEQAVRSVRLSLVAEVANNYLTLAADRERLKLARETLESQRVSYNLIKSRFSAGASSELDLRQAQTSVDSAR
VDVARYTSQVAQDENLLALVVGGPVPAELLPSGFEAAGMMRELSSGVPSEVLQRRPDIMEAEHLLKGANANIGAARAAFF
PRITLNTSIGTSSDQLSGLFSPGSFAWGFAPQFTLPIFDFGRNKAGLTVAERDRDIYLAQYEKSIQSAFREVADALAVNG
TITEQLDAQQSLVQATSESYRLSQARYMGGISSYLDVLVSQRSLYTTQQSLIGVNLTKLSNLVTLYKVLGGGSE
>Mature_474_residues
MKHAFLKKAFFLHLSGLLFLAGCSMAPNYTRPEAPVPQAWPSGPAYKGAASQALEPEAVQLKWEQFFINPQLRKLIKLAL
DNNRDLRIATLNVEKSRAQYRIQRADLLPSINGNGNATMERTPSDLSSSGEAITSHSYTLNLGVSSYEVDLFGRVQSLKD
QALEQFLSTEQAVRSVRLSLVAEVANNYLTLAADRERLKLARETLESQRVSYNLIKSRFSAGASSELDLRQAQTSVDSAR
VDVARYTSQVAQDENLLALVVGGPVPAELLPSGFEAAGMMRELSSGVPSEVLQRRPDIMEAEHLLKGANANIGAARAAFF
PRITLNTSIGTSSDQLSGLFSPGSFAWGFAPQFTLPIFDFGRNKAGLTVAERDRDIYLAQYEKSIQSAFREVADALAVNG
TITEQLDAQQSLVQATSESYRLSQARYMGGISSYLDVLVSQRSLYTTQQSLIGVNLTKLSNLVTLYKVLGGGSE

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=466, Percent_Identity=38.8412017167382, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI2367348, Length=461, Percent_Identity=24.7288503253796, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51788; Mature: 51788

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHAFLKKAFFLHLSGLLFLAGCSMAPNYTRPEAPVPQAWPSGPAYKGAASQALEPEAVQ
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHE
LKWEQFFINPQLRKLIKLALDNNRDLRIATLNVEKSRAQYRIQRADLLPSINGNGNATME
EEHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEC
RTPSDLSSSGEAITSHSYTLNLGVSSYEVDLFGRVQSLKDQALEQFLSTEQAVRSVRLSL
CCCCHHHCCCCEEECCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAEVANNYLTLAADRERLKLARETLESQRVSYNLIKSRFSAGASSELDLRQAQTSVDSAR
HHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VDVARYTSQVAQDENLLALVVGGPVPAELLPSGFEAAGMMRELSSGVPSEVLQRRPDIME
HHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHCCCCHHH
AEHLLKGANANIGAARAAFFPRITLNTSIGTSSDQLSGLFSPGSFAWGFAPQFTLPIFDF
HHHHHCCCCCCCCHHHHHHCCEEEEECCCCCCCHHHCCCCCCCCCCCCCCCCEECCHHHC
GRNKAGLTVAERDRDIYLAQYEKSIQSAFREVADALAVNGTITEQLDAQQSLVQATSESY
CCCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RLSQARYMGGISSYLDVLVSQRSLYTTQQSLIGVNLTKLSNLVTLYKVLGGGSE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKHAFLKKAFFLHLSGLLFLAGCSMAPNYTRPEAPVPQAWPSGPAYKGAASQALEPEAVQ
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHE
LKWEQFFINPQLRKLIKLALDNNRDLRIATLNVEKSRAQYRIQRADLLPSINGNGNATME
EEHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEC
RTPSDLSSSGEAITSHSYTLNLGVSSYEVDLFGRVQSLKDQALEQFLSTEQAVRSVRLSL
CCCCHHHCCCCEEECCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAEVANNYLTLAADRERLKLARETLESQRVSYNLIKSRFSAGASSELDLRQAQTSVDSAR
HHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VDVARYTSQVAQDENLLALVVGGPVPAELLPSGFEAAGMMRELSSGVPSEVLQRRPDIME
HHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHCCCCHHH
AEHLLKGANANIGAARAAFFPRITLNTSIGTSSDQLSGLFSPGSFAWGFAPQFTLPIFDF
HHHHHCCCCCCCCHHHHHHCCEEEEECCCCCCCHHHCCCCCCCCCCCCCCCCEECCHHHC
GRNKAGLTVAERDRDIYLAQYEKSIQSAFREVADALAVNGTITEQLDAQQSLVQATSESY
CCCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RLSQARYMGGISSYLDVLVSQRSLYTTQQSLIGVNLTKLSNLVTLYKVLGGGSE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]