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The map label for this gene is dnaN [H]

Identifier: 118578451

GI number: 118578451

Start: 1565

End: 2686

Strand: Direct

Name: dnaN [H]

Synonym: Ppro_0002

Alternate gene names: 118578451

Gene position: 1565-2686 (Clockwise)

Preceding gene: 118578450

Following gene: 118578452

Centisome position: 0.04

GC content: 53.39

Gene sequence:

>1122_bases
ATGGAATTCAGAATTGACAAGGAAGTCTTCCTCAAATCGCTTCAAAAGATACAGGGTATCGTCGAAAAGCGAACCTCCAT
GCCGATCCTGTCAAACGTATTGCTGGAAGCCACCGAATCGTCACTCCGCGTCACGGCAACCGATCTGGAAGTCGGAATGA
AATGCATCTACCCGGCTGAAATCCTCTCTCCGGGGAGAATCACCGTGGGAGCCAAGAAAATCTACGAAATCGTCAAGGAA
CTGCCCAACCAGCAGATCAGTTTCTCCGCCAAGGACAACGACTGGGTGGAGATAAGATGTGGCAAGGTTCATTTCAATAT
CGTCGGATTATCAGCCGAGGAATTTCCCTACTTCCCCGAAGTGCACGAGGAGAACCTGTTCGAAATCGAGAGCGCCCTGC
TTAAGGGAATGATCGAAAAGACCTCCTATGCCATCTGCAACGATGACACGAAGTACAACTTAAACGGCCTGTTCGCCAAG
ACGGAAATCAACGACGACGACGTACAGCTGCTCAAGATGGTTGCCACCGATGGCCACCGGCTCTCCATCGCCAGCTGCCC
GTTCAAGGGCAACGCGGGCCAGGAGCTTTCCAAGGGGGTCATCCTCCCCAAGAAGGGCGTCTACGAACTGAAGAAGATCG
CCGAGGAAGATGGCGGGACACTGATGTTCGGATTCATGGACAACAGCGCCGTCATCAAACGGGGCGATTCCTACATGGTC
ATGCGCCTGGTTGACGGAGAATTTCCCGACTACAACCGCGTCATTCCCACGTCGAATGACCGAAGCATCATCATCAACTG
TGACGACTTCACCCACTCGGTACGCAGGATGGCCATACTCTCCAGCGAAAAATTCAAGGGAATCATGCTGGATATATCCG
AATCCAGCATCAGGATCTCTTCCAGCAATCCCGAACTGGGAGACGCCATGGAGGAGATCGATGTCAGCTACGGCGGCGAG
CCGTTCTCGGTCAGATTCAACGCCCGCTACCTCCTGGACGTACTCGCCGTAACCGAGACCGAATCAGTGGAGATGAAATT
CAAGGATGAACTGGCGCCCTCCATCATCCTGCCGGACAAGAGCGACAGCTTCCTGGCCGTTATCATGCCCATGAGACTCT
GA

Upstream 100 bases:

>100_bases
GAATCCAAGACTTGAACGGTTATCACCAGATTCACAGCCCCTATAACAACAACAAAGCTTTTCTTTTTTAATAAAAGATA
CTGACAGTAAGGAGTGACCC

Downstream 100 bases:

>100_bases
CAGAAGGATTTGTGCGCCTCACCAGACTAAGTATCGCCGATTTCCGCAACATCGGATCCGTACGCTTCACGCCAGGCAGA
TGCTTCAACCTGATTCATGG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MEFRIDKEVFLKSLQKIQGIVEKRTSMPILSNVLLEATESSLRVTATDLEVGMKCIYPAEILSPGRITVGAKKIYEIVKE
LPNQQISFSAKDNDWVEIRCGKVHFNIVGLSAEEFPYFPEVHEENLFEIESALLKGMIEKTSYAICNDDTKYNLNGLFAK
TEINDDDVQLLKMVATDGHRLSIASCPFKGNAGQELSKGVILPKKGVYELKKIAEEDGGTLMFGFMDNSAVIKRGDSYMV
MRLVDGEFPDYNRVIPTSNDRSIIINCDDFTHSVRRMAILSSEKFKGIMLDISESSIRISSSNPELGDAMEEIDVSYGGE
PFSVRFNARYLLDVLAVTETESVEMKFKDELAPSIILPDKSDSFLAVIMPMRL

Sequences:

>Translated_373_residues
MEFRIDKEVFLKSLQKIQGIVEKRTSMPILSNVLLEATESSLRVTATDLEVGMKCIYPAEILSPGRITVGAKKIYEIVKE
LPNQQISFSAKDNDWVEIRCGKVHFNIVGLSAEEFPYFPEVHEENLFEIESALLKGMIEKTSYAICNDDTKYNLNGLFAK
TEINDDDVQLLKMVATDGHRLSIASCPFKGNAGQELSKGVILPKKGVYELKKIAEEDGGTLMFGFMDNSAVIKRGDSYMV
MRLVDGEFPDYNRVIPTSNDRSIIINCDDFTHSVRRMAILSSEKFKGIMLDISESSIRISSSNPELGDAMEEIDVSYGGE
PFSVRFNARYLLDVLAVTETESVEMKFKDELAPSIILPDKSDSFLAVIMPMRL
>Mature_373_residues
MEFRIDKEVFLKSLQKIQGIVEKRTSMPILSNVLLEATESSLRVTATDLEVGMKCIYPAEILSPGRITVGAKKIYEIVKE
LPNQQISFSAKDNDWVEIRCGKVHFNIVGLSAEEFPYFPEVHEENLFEIESALLKGMIEKTSYAICNDDTKYNLNGLFAK
TEINDDDVQLLKMVATDGHRLSIASCPFKGNAGQELSKGVILPKKGVYELKKIAEEDGGTLMFGFMDNSAVIKRGDSYMV
MRLVDGEFPDYNRVIPTSNDRSIIINCDDFTHSVRRMAILSSEKFKGIMLDISESSIRISSSNPELGDAMEEIDVSYGGE
PFSVRFNARYLLDVLAVTETESVEMKFKDELAPSIILPDKSDSFLAVIMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=378, Percent_Identity=36.2433862433862, Blast_Score=238, Evalue=4e-64,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41808; Mature: 41808

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFRIDKEVFLKSLQKIQGIVEKRTSMPILSNVLLEATESSLRVTATDLEVGMKCIYPAE
CCEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEHHHCEEEECHHH
ILSPGRITVGAKKIYEIVKELPNQQISFSAKDNDWVEIRCGKVHFNIVGLSAEEFPYFPE
HCCCCEEEECHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEEEEEEEECHHHCCCCCC
VHEENLFEIESALLKGMIEKTSYAICNDDTKYNLNGLFAKTEINDDDVQLLKMVATDGHR
CCCCHHHHHHHHHHHHHHHHCCEEEECCCCEECCCEEEEEECCCCHHHHHHHHHHCCCCE
LSIASCPFKGNAGQELSKGVILPKKGVYELKKIAEEDGGTLMFGFMDNSAVIKRGDSYMV
EEEEECCCCCCCCCHHHCCEECCCCCHHHHHHHHHCCCCEEEEEEECCCEEEECCCCEEE
MRLVDGEFPDYNRVIPTSNDRSIIINCDDFTHSVRRMAILSSEKFKGIMLDISESSIRIS
EEEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEE
SSNPELGDAMEEIDVSYGGEPFSVRFNARYLLDVLAVTETESVEMKFKDELAPSIILPDK
CCCCCHHHHHHHHHHHCCCCEEEEEECHHEEEEEHHHCCCCCEEEEECCCCCCEEEECCC
SDSFLAVIMPMRL
CCCEEEEEEECCC
>Mature Secondary Structure
MEFRIDKEVFLKSLQKIQGIVEKRTSMPILSNVLLEATESSLRVTATDLEVGMKCIYPAE
CCEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEHHHCEEEECHHH
ILSPGRITVGAKKIYEIVKELPNQQISFSAKDNDWVEIRCGKVHFNIVGLSAEEFPYFPE
HCCCCEEEECHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEEEEEEEEECHHHCCCCCC
VHEENLFEIESALLKGMIEKTSYAICNDDTKYNLNGLFAKTEINDDDVQLLKMVATDGHR
CCCCHHHHHHHHHHHHHHHHCCEEEECCCCEECCCEEEEEECCCCHHHHHHHHHHCCCCE
LSIASCPFKGNAGQELSKGVILPKKGVYELKKIAEEDGGTLMFGFMDNSAVIKRGDSYMV
EEEEECCCCCCCCCHHHCCEECCCCCHHHHHHHHHCCCCEEEEEEECCCEEEECCCCEEE
MRLVDGEFPDYNRVIPTSNDRSIIINCDDFTHSVRRMAILSSEKFKGIMLDISESSIRIS
EEEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEE
SSNPELGDAMEEIDVSYGGEPFSVRFNARYLLDVLAVTETESVEMKFKDELAPSIILPDK
CCCCCHHHHHHHHHHHCCCCEEEEEECHHEEEEEHHHCCCCCEEEEECCCCCCEEEECCC
SDSFLAVIMPMRL
CCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]