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The map label for this gene is trmE

Identifier: 118480511

GI number: 118480511

Start: 5253339

End: 5254715

Strand: Reverse

Name: trmE

Synonym: BALH_4991

Alternate gene names: 118480511

Gene position: 5254715-5253339 (Counterclockwise)

Preceding gene: 118480512

Following gene: 118480510

Centisome position: 99.95

GC content: 36.82

Gene sequence:

>1377_bases
ATGGAATTTGATACAATTGCCGCGATTTCCACAGCGCTTGGAGAGGGTGCAATTGCCATTGTTCGAGTAAGTGGAGATGA
TGCGGTTGAAAAAGTTAATCGTATTTTTAAAGGGAAGGATTTAACAGAGGTTCCTTCTCACACGATTCATTATGGTCATA
TTGTGGATTTAGATACAAATCAGGTTATTGAAGAAGTGATGGTATCTATTATGCGTGCACCAAGGACTTTTACACGTGAA
AATATAGTAGAAATTAACTGTCACGGTGGACTTGTTTCGGTAAATAAAGTATTGCAGCTTATTTTAGCGCAAGGGGTACG
ATTAGCAGAGCCTGGTGAATTTACAAAACGTGCTTTTTTAAATGGCCGTATTGATTTATCACAAGCAGAAGCTGTTATGG
ACTTAATCCGTGCAAAAACAGATCGTGCAATGAACGTAGCGATTAATCAAATGGAAGGACGATTATCTAAATTAATCGGT
CGTCTGCGTCAAGATATATTAGAAACGTTAGCTCATGTTGAGGTAAACATAGATTACCCAGAATATGATGATGTGGAAGA
AATGACACATAATATTTTAATCGAGAAAGCTACACATGTTCGTTCTGAAATTGCAAAGATATTAGAAACATCGAAGCAAG
GAAAGATTTTACGTGAAGGTATTGCTACTGCAATCATCGGTAGACCTAATGTTGGGAAATCATCGCTATTAAATAGTCTC
GTTCAGGAGAAAAAGGCAATTGTAACTGATATTGCAGGAACAACTCGTGATGTTATTGAAGAGTACGTTAATGTGCGTGG
TGTACCACTTAAACTTATAGATACAGCGGGGATTCGTGAAACGGAAGATGTTGTTGAACGAATTGGCGTAGAGCGTTCAA
AAGAAATGATGAGTCAAGCTGATTTAGTGTTAGTTGTCGTTAATTATAGTGAAGCTTTAACGAATGAGGATGAAGAGCTA
TTCCGTGCAGTACAAGGAAAAGATTTCATTGTTATTGTAAATAAGACAGATTTACCGCAAGCAATTGATATGGAACGTGT
TATAGAATTGGCAGCGGGAAATCGTGTTATTACAACATCGTTAATTGAGGAACAAGGAATAGATGAGCTTGAAAAGGCAA
TAGCTGATTTATTTTTTGAAGGAACAATCGATTCTGCAGATGTAACATATGTTTCTAATGCGAGACATATTGGATTATTA
ACACAAGCAGGAAAAACGATTGGAGATGCAATTGAGGCGATAGAAAATGGTGTTCCAATTGATATGGTACAAATCGATTT
AACAAGAACGTGGGAAATACTTGGTGAAATTACTGGTGATACCGTTCATGAAAGCCTAATTGACCAGTTGTTCTCTCAAT
TTTGTTTAGGTAAATAA

Upstream 100 bases:

>100_bases
TTATTCTTTTGTAGATAGGTTCGTTATGGTATTCTAATAATTTAGTGAAGAAAATAATCATGTTGAAATAGAGATAAATA
AGCAAGTGAGGTGAAAGGAC

Downstream 100 bases:

>100_bases
TATAGTGGTGTTTCAGAAAGATAGAGATTATTAGGAGGAATAAACAATGGGATACAATGCCGGTTCATACGATGTCATAG
TAATCGGTGCAGGTCATGCA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MEFDTIAAISTALGEGAIAIVRVSGDDAVEKVNRIFKGKDLTEVPSHTIHYGHIVDLDTNQVIEEVMVSIMRAPRTFTRE
NIVEINCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIG
RLRQDILETLAHVEVNIDYPEYDDVEEMTHNILIEKATHVRSEIAKILETSKQGKILREGIATAIIGRPNVGKSSLLNSL
VQEKKAIVTDIAGTTRDVIEEYVNVRGVPLKLIDTAGIRETEDVVERIGVERSKEMMSQADLVLVVVNYSEALTNEDEEL
FRAVQGKDFIVIVNKTDLPQAIDMERVIELAAGNRVITTSLIEEQGIDELEKAIADLFFEGTIDSADVTYVSNARHIGLL
TQAGKTIGDAIEAIENGVPIDMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK

Sequences:

>Translated_458_residues
MEFDTIAAISTALGEGAIAIVRVSGDDAVEKVNRIFKGKDLTEVPSHTIHYGHIVDLDTNQVIEEVMVSIMRAPRTFTRE
NIVEINCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIG
RLRQDILETLAHVEVNIDYPEYDDVEEMTHNILIEKATHVRSEIAKILETSKQGKILREGIATAIIGRPNVGKSSLLNSL
VQEKKAIVTDIAGTTRDVIEEYVNVRGVPLKLIDTAGIRETEDVVERIGVERSKEMMSQADLVLVVVNYSEALTNEDEEL
FRAVQGKDFIVIVNKTDLPQAIDMERVIELAAGNRVITTSLIEEQGIDELEKAIADLFFEGTIDSADVTYVSNARHIGLL
TQAGKTIGDAIEAIENGVPIDMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK
>Mature_458_residues
MEFDTIAAISTALGEGAIAIVRVSGDDAVEKVNRIFKGKDLTEVPSHTIHYGHIVDLDTNQVIEEVMVSIMRAPRTFTRE
NIVEINCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIG
RLRQDILETLAHVEVNIDYPEYDDVEEMTHNILIEKATHVRSEIAKILETSKQGKILREGIATAIIGRPNVGKSSLLNSL
VQEKKAIVTDIAGTTRDVIEEYVNVRGVPLKLIDTAGIRETEDVVERIGVERSKEMMSQADLVLVVVNYSEALTNEDEEL
FRAVQGKDFIVIVNKTDLPQAIDMERVIELAAGNRVITTSLIEEQGIDELEKAIADLFFEGTIDSADVTYVSNARHIGLL
TQAGKTIGDAIEAIENGVPIDMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=479, Percent_Identity=30.4801670146138, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI193082988, Length=508, Percent_Identity=28.5433070866142, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI193082993, Length=468, Percent_Identity=30.5555555555556, Blast_Score=192, Evalue=5e-49,
Organism=Escherichia coli, GI2367268, Length=464, Percent_Identity=33.4051724137931, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI87082120, Length=122, Percent_Identity=37.7049180327869, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=465, Percent_Identity=29.247311827957, Blast_Score=185, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=30.0202839756592, Blast_Score=205, Evalue=2e-53,
Organism=Drosophila melanogaster, GI45550813, Length=477, Percent_Identity=31.0272536687631, Blast_Score=201, Evalue=7e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_BACAH (A0RLR2)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_897662.1
- ProteinModelPortal:   A0RLR2
- SMR:   A0RLR2
- STRING:   A0RLR2
- EnsemblBacteria:   EBBACT00000070585
- GeneID:   4544398
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_4991
- NMPDR:   fig|412694.5.peg.4790
- eggNOG:   COG0486
- GeneTree:   EBGT00070000032012
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   BTHU412694:BALH_4991-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 50562; Mature: 50562

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: BINDING 22-22 BINDING 84-84 BINDING 123-123 BINDING 458-458

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFDTIAAISTALGEGAIAIVRVSGDDAVEKVNRIFKGKDLTEVPSHTIHYGHIVDLDTN
CCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEECEEEECCHH
QVIEEVMVSIMRAPRTFTRENIVEINCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAFL
HHHHHHHHHHHHCCCHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
NGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIGRLRQDILETLAHVEVNIDYP
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
EYDDVEEMTHNILIEKATHVRSEIAKILETSKQGKILREGIATAIIGRPNVGKSSLLNSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
VQEKKAIVTDIAGTTRDVIEEYVNVRGVPLKLIDTAGIRETEDVVERIGVERSKEMMSQA
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHCCC
DLVLVVVNYSEALTNEDEELFRAVQGKDFIVIVNKTDLPQAIDMERVIELAAGNRVITTS
CEEEEEEECHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCHHCHHHHHHHHCCCEEEEHH
LIEEQGIDELEKAIADLFFEGTIDSADVTYVSNARHIGLLTQAGKTIGDAIEAIENGVPI
HHHHCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEHHHHCCHHHHHHHHHHHCCCCE
DMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK
EEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEFDTIAAISTALGEGAIAIVRVSGDDAVEKVNRIFKGKDLTEVPSHTIHYGHIVDLDTN
CCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEECEEEECCHH
QVIEEVMVSIMRAPRTFTRENIVEINCHGGLVSVNKVLQLILAQGVRLAEPGEFTKRAFL
HHHHHHHHHHHHCCCHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
NGRIDLSQAEAVMDLIRAKTDRAMNVAINQMEGRLSKLIGRLRQDILETLAHVEVNIDYP
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
EYDDVEEMTHNILIEKATHVRSEIAKILETSKQGKILREGIATAIIGRPNVGKSSLLNSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
VQEKKAIVTDIAGTTRDVIEEYVNVRGVPLKLIDTAGIRETEDVVERIGVERSKEMMSQA
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHCCC
DLVLVVVNYSEALTNEDEELFRAVQGKDFIVIVNKTDLPQAIDMERVIELAAGNRVITTS
CEEEEEEECHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCHHCHHHHHHHHCCCEEEEHH
LIEEQGIDELEKAIADLFFEGTIDSADVTYVSNARHIGLLTQAGKTIGDAIEAIENGVPI
HHHHCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEHHHHCCHHHHHHHHHHHCCCCE
DMVQIDLTRTWEILGEITGDTVHESLIDQLFSQFCLGK
EEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA