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The map label for this gene is purA [H]

Identifier: 118480495

GI number: 118480495

Start: 5236983

End: 5238362

Strand: Reverse

Name: purA [H]

Synonym: BALH_4974

Alternate gene names: 118480495

Gene position: 5238362-5236983 (Counterclockwise)

Preceding gene: 118480496

Following gene: 118480494

Centisome position: 99.64

GC content: 38.77

Gene sequence:

>1380_bases
ATGTTCGCTTTTTGGTTGACTTCTTTTTCCGTTTTGGTACAATTATATTGTTTGAATAGATCGTTTGAAAATTGCGGAGG
TGCTTTAATAATGTCTTCAGTAGTAGTTGTAGGAACACAATGGGGCGACGAAGGAAAAGGTAAAATTACTGATTTTCTTT
CTGAGCATGCGGAAGTAGTTGCAAGATATCAAGGTGGAAATAACGCAGGACATACAATCGTTTTTGGTGGAGTTAAATAT
AAATTACACTTAATTCCATCTGGTATTTTCTATAAAGAGAAAATTTGTGTAATCGGAAACGGCTTAGTAGTAGATCCGAA
AGCATTACTTGAAGAGTTAAAATACTTACACGATCGTGGTGTAAGTACTGATAATTTACGCGTAAGTAACCGTGCGCACG
TTATTTTACCTTATCACTTAAAACAGGACGAGTTAGAAGAAGCAAGTAAAGGTGATAACAAAATCGGTACAACGAAAAAA
GGTATCGGTCCTGCATATATGGACAAAGCTGCTCGTATCGGTATTCGTATGGCTGATCTTTTAGACCGTGAAGCATTTAA
AGAGAAGCTTGAGCAAAATTTAGCACAAAAAAATCGTTTATTTGAAAAAATGTACGATACAGAAGGTTTCAGTGTAGACG
AAATCTTCGAAGAGTACTTCGAGTACGGACAGCAAATCGCACAATATGTATGTGATACGTCTGTTGTATTAAATGATGCA
TTAGATAACAATCACCGTGTATTATTTGAAGGTGCACAAGGTGTTATGCTTGATATCGACCACGGTACGTACCCGTTCGT
TACATCTTCTAACCCAATTGCTGGTGGTGTAACAGTTGGAACTGGAGTTGGTCCTGCGAAAGTTACTCGTGTTGTAGGTG
TATGTAAAGCATATACAAGCCGCGTTGGTGACGGTCCATTCCCTACTGAGCTTCATGACGAAATTGGTCATCAAATTCGT
GAAGTTGGTCGTGAGTATGGAACGACAACTGGTCGTCCACGCCGCGTAGGTTGGTTCGATAGCGTTGTTGTAAGACACGC
ACGTCGTGTTAGTGGTTTAACAGATCTATCATTAAACTCTATCGACGTTCTAACTGGTATTCCAACACTTAAAATTTGTG
TTGCTTACAAATGCGATGGCAAAGTTATCGATGAAGTTCCAGCAAACTTAAACATTTTAGCGAAATGTGAGCCTGTATAC
GAAGAGCTTCCAGGTTGGACAGAAGATATTACTGGTGTAAGATCATTAGATGAGCTTCCTGAAAATGCACGAAAATACGT
AGAACGTGTTTCTGAGTTAACAGGAATTCAATTATCTATGTTCTCAGTTGGACCAGATCGTAACCAAACAAATATCGTTC
GTAACGTATACGAAGCTTAA

Upstream 100 bases:

>100_bases
TCTTATAATAAGATGTGTTTCTTTTTTTTTATATAAAAGATAAGGCTTTCATAATGTAAAAATATAATTCTTACGAACGA
AAAAGTTTTTTTATAAAAAA

Downstream 100 bases:

>100_bases
TTGATATTTTTGAGAAAAACTCATGTCCGCATGAGTTTTTTCTTATCTTTTATAAAAAAATAAAAAAAGGTATTGCGAAA
TATAAAGAAAATATGTTATG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MFAFWLTSFSVLVQLYCLNRSFENCGGALIMSSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFGGVKY
KLHLIPSGIFYKEKICVIGNGLVVDPKALLEELKYLHDRGVSTDNLRVSNRAHVILPYHLKQDELEEASKGDNKIGTTKK
GIGPAYMDKAARIGIRMADLLDREAFKEKLEQNLAQKNRLFEKMYDTEGFSVDEIFEEYFEYGQQIAQYVCDTSVVLNDA
LDNNHRVLFEGAQGVMLDIDHGTYPFVTSSNPIAGGVTVGTGVGPAKVTRVVGVCKAYTSRVGDGPFPTELHDEIGHQIR
EVGREYGTTTGRPRRVGWFDSVVVRHARRVSGLTDLSLNSIDVLTGIPTLKICVAYKCDGKVIDEVPANLNILAKCEPVY
EELPGWTEDITGVRSLDELPENARKYVERVSELTGIQLSMFSVGPDRNQTNIVRNVYEA

Sequences:

>Translated_459_residues
MFAFWLTSFSVLVQLYCLNRSFENCGGALIMSSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFGGVKY
KLHLIPSGIFYKEKICVIGNGLVVDPKALLEELKYLHDRGVSTDNLRVSNRAHVILPYHLKQDELEEASKGDNKIGTTKK
GIGPAYMDKAARIGIRMADLLDREAFKEKLEQNLAQKNRLFEKMYDTEGFSVDEIFEEYFEYGQQIAQYVCDTSVVLNDA
LDNNHRVLFEGAQGVMLDIDHGTYPFVTSSNPIAGGVTVGTGVGPAKVTRVVGVCKAYTSRVGDGPFPTELHDEIGHQIR
EVGREYGTTTGRPRRVGWFDSVVVRHARRVSGLTDLSLNSIDVLTGIPTLKICVAYKCDGKVIDEVPANLNILAKCEPVY
EELPGWTEDITGVRSLDELPENARKYVERVSELTGIQLSMFSVGPDRNQTNIVRNVYEA
>Mature_459_residues
MFAFWLTSFSVLVQLYCLNRSFENCGGALIMSSVVVVGTQWGDEGKGKITDFLSEHAEVVARYQGGNNAGHTIVFGGVKY
KLHLIPSGIFYKEKICVIGNGLVVDPKALLEELKYLHDRGVSTDNLRVSNRAHVILPYHLKQDELEEASKGDNKIGTTKK
GIGPAYMDKAARIGIRMADLLDREAFKEKLEQNLAQKNRLFEKMYDTEGFSVDEIFEEYFEYGQQIAQYVCDTSVVLNDA
LDNNHRVLFEGAQGVMLDIDHGTYPFVTSSNPIAGGVTVGTGVGPAKVTRVVGVCKAYTSRVGDGPFPTELHDEIGHQIR
EVGREYGTTTGRPRRVGWFDSVVVRHARRVSGLTDLSLNSIDVLTGIPTLKICVAYKCDGKVIDEVPANLNILAKCEPVY
EELPGWTEDITGVRSLDELPENARKYVERVSELTGIQLSMFSVGPDRNQTNIVRNVYEA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=422, Percent_Identity=42.8909952606635, Blast_Score=335, Evalue=8e-92,
Organism=Homo sapiens, GI22748717, Length=421, Percent_Identity=43.2304038004751, Blast_Score=331, Evalue=8e-91,
Organism=Homo sapiens, GI40316944, Length=392, Percent_Identity=42.0918367346939, Blast_Score=291, Evalue=6e-79,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=46.9767441860465, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25146553, Length=448, Percent_Identity=41.7410714285714, Blast_Score=330, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=42.1911421911422, Blast_Score=329, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6324109, Length=423, Percent_Identity=41.371158392435, Blast_Score=340, Evalue=4e-94,
Organism=Drosophila melanogaster, GI21356033, Length=417, Percent_Identity=42.9256594724221, Blast_Score=334, Evalue=6e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 50837; Mature: 50837

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00178 AA_TRNA_LIGASE_I ; PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAFWLTSFSVLVQLYCLNRSFENCGGALIMSSVVVVGTQWGDEGKGKITDFLSEHAEVV
CEEHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHH
ARYQGGNNAGHTIVFGGVKYKLHLIPSGIFYKEKICVIGNGLVVDPKALLEELKYLHDRG
HHCCCCCCCCCEEEECCEEEEEEEECCCCEEECEEEEEECCEEECHHHHHHHHHHHHHCC
VSTDNLRVSNRAHVILPYHLKQDELEEASKGDNKIGTTKKGIGPAYMDKAARIGIRMADL
CCCCCEEECCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHH
LDREAFKEKLEQNLAQKNRLFEKMYDTEGFSVDEIFEEYFEYGQQIAQYVCDTSVVLNDA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHC
LDNNHRVLFEGAQGVMLDIDHGTYPFVTSSNPIAGGVTVGTGVGPAKVTRVVGVCKAYTS
CCCCCEEEEECCCEEEEEECCCCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
RVGDGPFPTELHDEIGHQIREVGREYGTTTGRPRRVGWFDSVVVRHARRVSGLTDLSLNS
HCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
IDVLTGIPTLKICVAYKCDGKVIDEVPANLNILAKCEPVYEELPGWTEDITGVRSLDELP
CHHHHCCCCEEEEEEEECCCCCHHCCCCCCEEEEECCHHHHHCCCCHHHHHHHHHHHHCH
ENARKYVERVSELTGIQLSMFSVGPDRNQTNIVRNVYEA
HHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MFAFWLTSFSVLVQLYCLNRSFENCGGALIMSSVVVVGTQWGDEGKGKITDFLSEHAEVV
CEEHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHH
ARYQGGNNAGHTIVFGGVKYKLHLIPSGIFYKEKICVIGNGLVVDPKALLEELKYLHDRG
HHCCCCCCCCCEEEECCEEEEEEEECCCCEEECEEEEEECCEEECHHHHHHHHHHHHHCC
VSTDNLRVSNRAHVILPYHLKQDELEEASKGDNKIGTTKKGIGPAYMDKAARIGIRMADL
CCCCCEEECCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHH
LDREAFKEKLEQNLAQKNRLFEKMYDTEGFSVDEIFEEYFEYGQQIAQYVCDTSVVLNDA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHC
LDNNHRVLFEGAQGVMLDIDHGTYPFVTSSNPIAGGVTVGTGVGPAKVTRVVGVCKAYTS
CCCCCEEEEECCCEEEEEECCCCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
RVGDGPFPTELHDEIGHQIREVGREYGTTTGRPRRVGWFDSVVVRHARRVSGLTDLSLNS
HCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
IDVLTGIPTLKICVAYKCDGKVIDEVPANLNILAKCEPVYEELPGWTEDITGVRSLDELP
CHHHHCCCCEEEEEEEECCCCCHHCCCCCCEEEEECCHHHHHCCCCHHHHHHHHHHHHCH
ENARKYVERVSELTGIQLSMFSVGPDRNQTNIVRNVYEA
HHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA