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The map label for this gene is nos [H]

Identifier: 118480474

GI number: 118480474

Start: 5214485

End: 5215555

Strand: Reverse

Name: nos [H]

Synonym: BALH_4953

Alternate gene names: 118480474

Gene position: 5215555-5214485 (Counterclockwise)

Preceding gene: 118480476

Following gene: 118480473

Centisome position: 99.21

GC content: 37.54

Gene sequence:

>1071_bases
ATGAGTAAAACGAAACAATTAATAGAGGAAGCGAGTCATTTTATTACGATTTGCTATAAAGAGCTTAGTAAAGAACATTT
CATAGAAGAACGCATGAAAGAAATTCAAGCTGAGATAGAGAAGACAGGGACATATGAGCATACATTTGAAGAACTTGTTC
ATGGATCGCGAATGGCATGGCGCAATAGTAATCGATGTATCGGAAGACTATTTTGGAGTAAGATGCACATATTAGATGCA
CGTGAAGTAAATGATGAGGAAGGCGTATATCATGCATTAATTCATCATATTAAATATGCAACGAACGATGGAAAAGTGAA
ACCGACAATTACGATTTTTAAGCAATATGAAGGTGAAGAAAATAATATACGAATTTATAATCATCAATTAATTCGATATG
CAGGATATAAAACAGAAATGGGAGTGATTGGTGACTCTCATTCCGCTGCATTTACGGATTTTTGTCAGGAGCTTGGCTGG
CAAGGAGAAGGCACGAATTTCGATGTATTGCCACTGGTGTTTTCTGTTGATGGAAAAGAACCTGTATATAAAGAAATTCC
TAAAAACGAAGTGAAAGAAGTACCAATTGAACATCCAGAGTACCCAATTTCATCTTTAGGAGTCAAATGGTATGGAGTAC
CGATGATTTCAGATATGCGCTTAGAAATTGGTGGTATTTCCTATACAGCAGCTCCGTTTAATGGATGGTACATGGGTACG
GAAATTGGGGCGCGTAACTTAGCGGATCATGATCGTTATAATTTACTGCCCGCCGTTGCGGAGATGATGAAGCTAGATAC
ATCGAGAAACGGTACATTATGGAAAGACAAGGCATTAATTGAATTAAACGTGGCTGTTTTACATTCCTTTAAAAAACAAG
GAGTTAGTATTGTTGATCATCATACTGCTGCACAACAATTTCAGCAATTTGAGAAGCAAGAAGCTGCTTGTGGTCGTGTT
GTAACCGGCAATTGGGTGTGGCTCATTCCACCGTTATCTCCAGCTACCACTCATATTTATCATAAACCGTATCCAAATGA
AATTTTGAAGCCGAATTTCTTTCATAAATAG

Upstream 100 bases:

>100_bases
TTACAATAAAAGTAACACTGCGACTTTCATTTGTGACTCTCATTCTTTTTGCTTATACTGTAAAGTAGTAATGTTTTGTT
GAAATAGAAGGAGATCGTTT

Downstream 100 bases:

>100_bases
TTTGTAGTATAAGGCATTTTCTATAAAAATATAATTTTTTTAATTACTGTTACGTAGCAAATGGAAGCCTTTACTCTCGG
TTATTTTTGGAAGGAGCTTT

Product: nitric-oxide synthase, oxygenase subunit

Products: citrulline; nitric oxide; NADP+

Alternate protein names: NOSoxy-like protein [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSKTKQLIEEASHFITICYKELSKEHFIEERMKEIQAEIEKTGTYEHTFEELVHGSRMAWRNSNRCIGRLFWSKMHILDA
REVNDEEGVYHALIHHIKYATNDGKVKPTITIFKQYEGEENNIRIYNHQLIRYAGYKTEMGVIGDSHSAAFTDFCQELGW
QGEGTNFDVLPLVFSVDGKEPVYKEIPKNEVKEVPIEHPEYPISSLGVKWYGVPMISDMRLEIGGISYTAAPFNGWYMGT
EIGARNLADHDRYNLLPAVAEMMKLDTSRNGTLWKDKALIELNVAVLHSFKKQGVSIVDHHTAAQQFQQFEKQEAACGRV
VTGNWVWLIPPLSPATTHIYHKPYPNEILKPNFFHK

Sequences:

>Translated_356_residues
MSKTKQLIEEASHFITICYKELSKEHFIEERMKEIQAEIEKTGTYEHTFEELVHGSRMAWRNSNRCIGRLFWSKMHILDA
REVNDEEGVYHALIHHIKYATNDGKVKPTITIFKQYEGEENNIRIYNHQLIRYAGYKTEMGVIGDSHSAAFTDFCQELGW
QGEGTNFDVLPLVFSVDGKEPVYKEIPKNEVKEVPIEHPEYPISSLGVKWYGVPMISDMRLEIGGISYTAAPFNGWYMGT
EIGARNLADHDRYNLLPAVAEMMKLDTSRNGTLWKDKALIELNVAVLHSFKKQGVSIVDHHTAAQQFQQFEKQEAACGRV
VTGNWVWLIPPLSPATTHIYHKPYPNEILKPNFFHK
>Mature_355_residues
SKTKQLIEEASHFITICYKELSKEHFIEERMKEIQAEIEKTGTYEHTFEELVHGSRMAWRNSNRCIGRLFWSKMHILDAR
EVNDEEGVYHALIHHIKYATNDGKVKPTITIFKQYEGEENNIRIYNHQLIRYAGYKTEMGVIGDSHSAAFTDFCQELGWQ
GEGTNFDVLPLVFSVDGKEPVYKEIPKNEVKEVPIEHPEYPISSLGVKWYGVPMISDMRLEIGGISYTAAPFNGWYMGTE
IGARNLADHDRYNLLPAVAEMMKLDTSRNGTLWKDKALIELNVAVLHSFKKQGVSIVDHHTAAQQFQQFEKQEAACGRVV
TGNWVWLIPPLSPATTHIYHKPYPNEILKPNFFHK

Specific function: Catalyzes the production of nitric oxide [H]

COG id: COG4362

COG function: function code PE; Nitric oxide synthase, oxygenase domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NOS family. Bacterial NOS oxygenase subfamily [H]

Homologues:

Organism=Homo sapiens, GI10835173, Length=354, Percent_Identity=43.7853107344633, Blast_Score=331, Evalue=6e-91,
Organism=Homo sapiens, GI24041029, Length=356, Percent_Identity=41.5730337078652, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI40254422, Length=358, Percent_Identity=41.8994413407821, Blast_Score=314, Evalue=1e-85,
Organism=Homo sapiens, GI231571314, Length=356, Percent_Identity=41.8539325842697, Blast_Score=312, Evalue=2e-85,
Organism=Homo sapiens, GI231571356, Length=356, Percent_Identity=41.8539325842697, Blast_Score=312, Evalue=2e-85,
Organism=Homo sapiens, GI231571329, Length=356, Percent_Identity=41.8539325842697, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI78706868, Length=355, Percent_Identity=43.3802816901408, Blast_Score=336, Evalue=1e-92,
Organism=Drosophila melanogaster, GI78706866, Length=355, Percent_Identity=43.3802816901408, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24583543, Length=355, Percent_Identity=43.3802816901408, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI78706876, Length=355, Percent_Identity=43.3802816901408, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI78706882, Length=355, Percent_Identity=43.3802816901408, Blast_Score=335, Evalue=2e-92,
Organism=Drosophila melanogaster, GI78706870, Length=355, Percent_Identity=43.3802816901408, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI78706878, Length=355, Percent_Identity=43.3802816901408, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI78706880, Length=355, Percent_Identity=43.3802816901408, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI78706872, Length=154, Percent_Identity=41.5584415584416, Blast_Score=131, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017142
- InterPro:   IPR004030 [H]

Pfam domain/function: PF02898 NO_synthase [H]

EC number: 1.14.13.39

Molecular weight: Translated: 40987; Mature: 40856

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS60001 NOS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTKQLIEEASHFITICYKELSKEHFIEERMKEIQAEIEKTGTYEHTFEELVHGSRMAW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHEE
RNSNRCIGRLFWSKMHILDAREVNDEEGVYHALIHHIKYATNDGKVKPTITIFKQYEGEE
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCC
NNIRIYNHQLIRYAGYKTEMGVIGDSHSAAFTDFCQELGWQGEGTNFDVLPLVFSVDGKE
CCEEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCC
PVYKEIPKNEVKEVPIEHPEYPISSLGVKWYGVPMISDMRLEIGGISYTAAPFNGWYMGT
CHHHHCCHHHHHCCCCCCCCCCHHHCCEEEECCCEEECCEEEECCEEEEECCCCCEEEEC
EIGARNLADHDRYNLLPAVAEMMKLDTSRNGTLWKDKALIELNVAVLHSFKKQGVSIVDH
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCEEEEEHHHHHHHHHHCCCEEEHH
HTAAQQFQQFEKQEAACGRVVTGNWVWLIPPLSPATTHIYHKPYPNEILKPNFFHK
HHHHHHHHHHHHHHHHCCCEEECCEEEEECCCCCCCCEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKTKQLIEEASHFITICYKELSKEHFIEERMKEIQAEIEKTGTYEHTFEELVHGSRMAW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHEE
RNSNRCIGRLFWSKMHILDAREVNDEEGVYHALIHHIKYATNDGKVKPTITIFKQYEGEE
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCC
NNIRIYNHQLIRYAGYKTEMGVIGDSHSAAFTDFCQELGWQGEGTNFDVLPLVFSVDGKE
CCEEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCC
PVYKEIPKNEVKEVPIEHPEYPISSLGVKWYGVPMISDMRLEIGGISYTAAPFNGWYMGT
CHHHHCCHHHHHCCCCCCCCCCHHHCCEEEECCCEEECCEEEECCEEEEECCCCCEEEEC
EIGARNLADHDRYNLLPAVAEMMKLDTSRNGTLWKDKALIELNVAVLHSFKKQGVSIVDH
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCEEEEEHHHHHHHHHHCCCEEEHH
HTAAQQFQQFEKQEAACGRVVTGNWVWLIPPLSPATTHIYHKPYPNEILKPNFFHK
HHHHHHHHHHHHHHHHCCCEEECCEEEEECCCCCCCCEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-arginine; NADPH; H+; O2

Specific reaction: L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 11856757; 12220171 [H]