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The map label for this gene is metG

Identifier: 118480122

GI number: 118480122

Start: 4808337

End: 4809971

Strand: Reverse

Name: metG

Synonym: BALH_4573

Alternate gene names: 118480122

Gene position: 4809971-4808337 (Counterclockwise)

Preceding gene: 118480127

Following gene: 118480116

Centisome position: 91.49

GC content: 37.55

Gene sequence:

>1635_bases
ATGAGTATTTTTATTGGGGGCGCTTGGCCGTATGCAAATGGTTCGTTACATCTTGGACATATTGCGAGTTTGTTACCTGG
AGATATTTTAGCACGTTATTACCGGGCAAAAGGTGAGAATGTGCTGTATGTTTCGGGAAGTGATTGTAATGGAACACCAA
TTGCAATTCGGGCAAAACAAGAAGGTGTGACGGCGAAGGAAATTGCTAATAAGTATCATGAAGAGTTTGAGCGATGTTTT
CGTAACCTTGGTTTTACGTATGATTGCTATACACGTACAGATAGTGAGCACCATCATGAAACGGTTCAAAATGTTTTTTT
ACGTTTATTAGAAGAAGGACATATTTATAAAAAAACAGTAGAACAAGCATATTGCGAAACGTGTACACAGTTTTTACCTG
ATCGTTATGTAGAAGGTGTTTGTCCACATTGCCATGAAGAGGCAAGAGGAGATCAATGCGATGCGTGTTCAGCTATTTTA
GATCCACTCGATTTGTTAGAAAAGAAATGTAAGTTATGTGGTAGTACACCATCCATACAGGAAACAGAGCATTTCTATTT
CGCATTGCATACATTTCAGGAGCAAATTAAAAGGGCTGTAGAAATCGCTAAACAAACAGGAACATGGCGCGACAATGCGA
TTCAACTAACAGAGAGATATGTAAAGGAAGGATTACTAGATAGGGCCGTATCACGTGATTTACCAATCGGGGTACCAATT
CCAGTAGAAGGGTATGAGGATAAGAAAATTTACGTATGGATTGAAGCAGTGACAGGGTATTATTCAGCAAGTAAACATTG
GGCTGAAGAAACAGGGAAAGATGATCGAGAGTTTTGGGATAGCGAAGCGAAAACATATTATGTGCACGGAAAGGATAATA
TTCCGTTTCATTCCGTCATTTGGCCAGCAGTATTACTTGGAATAGGAGAAGGGGCAATTCCTCGTCATATCGTTTCGAAT
GAATATTTAACAGTTGAAAAAAGAAAATTATCTACGAGTAAAAACTGGGCAGTATGGGTGCCTGATATATTAGAACGCTA
TGATCCAGATTCTATTCGTTACTTTTTAACAGTAAATGCACCAGAAAATCGTGACACTGATTTTTCATGGCGTGAATTTA
TTTACAGTCATAATAGTGAATTGTTAGGTGCATACGGGAACTTTGTGAACCGAACATTAAAGTTTATTGAAAAATATTAT
GGTGGTATCATGCCGAAGGGAAGTATTGATGTAGAGCTAAAAGATAAGATAGAAAGATTATATAAACATGTAGGAGAGGC
GATTGAGCAAACTAAATTTAAGGTGGCATTAGAATCAATATTTGATGCAGTACGTTTTGCAAATAAATACTTTGATGAGA
GGCAGCCGTGGAAAGAAAGGGAAGATGATCCAGTTTCATGCGAAGAAACGATTTATAATTGTGTGTATCTCATCGCTAAT
TTTGCAAATCTCCTGGAACCATTTTTACCATTTTCAAGTGAAAGAATTAGAAACACATTATCGATTGTGAACCGAAATTG
GGAGCCTCAACATACGTTGCCGACTAGAATTGATAGTGTAAAGCCACTATTTGAACGAATCGATGTAAAACAAATTGAGC
GTGAGTTAGAAAAGTTATATGGAGCGGTAAAGTGA

Upstream 100 bases:

>100_bases
TTGGCGAAATAAGGTGGTACCGCGACTGTTTATACAGCCCCGCCCTTATCTTTTTTAAGATAGGGGCGGGGCTTTTTATA
TTTTAAAGGAGGAATGTAAG

Downstream 100 bases:

>100_bases
GATAGAGAAAAAGACCTTTCAAATGCATGAAAGGTCTTTTGTTATGCACTATGCACGCTACTATGTTTCTTTTCTTTCCT
AGCGGTAAGTAGCTGAGGGG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase 1; MetRS 1

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MSIFIGGAWPYANGSLHLGHIASLLPGDILARYYRAKGENVLYVSGSDCNGTPIAIRAKQEGVTAKEIANKYHEEFERCF
RNLGFTYDCYTRTDSEHHHETVQNVFLRLLEEGHIYKKTVEQAYCETCTQFLPDRYVEGVCPHCHEEARGDQCDACSAIL
DPLDLLEKKCKLCGSTPSIQETEHFYFALHTFQEQIKRAVEIAKQTGTWRDNAIQLTERYVKEGLLDRAVSRDLPIGVPI
PVEGYEDKKIYVWIEAVTGYYSASKHWAEETGKDDREFWDSEAKTYYVHGKDNIPFHSVIWPAVLLGIGEGAIPRHIVSN
EYLTVEKRKLSTSKNWAVWVPDILERYDPDSIRYFLTVNAPENRDTDFSWREFIYSHNSELLGAYGNFVNRTLKFIEKYY
GGIMPKGSIDVELKDKIERLYKHVGEAIEQTKFKVALESIFDAVRFANKYFDERQPWKEREDDPVSCEETIYNCVYLIAN
FANLLEPFLPFSSERIRNTLSIVNRNWEPQHTLPTRIDSVKPLFERIDVKQIERELEKLYGAVK

Sequences:

>Translated_544_residues
MSIFIGGAWPYANGSLHLGHIASLLPGDILARYYRAKGENVLYVSGSDCNGTPIAIRAKQEGVTAKEIANKYHEEFERCF
RNLGFTYDCYTRTDSEHHHETVQNVFLRLLEEGHIYKKTVEQAYCETCTQFLPDRYVEGVCPHCHEEARGDQCDACSAIL
DPLDLLEKKCKLCGSTPSIQETEHFYFALHTFQEQIKRAVEIAKQTGTWRDNAIQLTERYVKEGLLDRAVSRDLPIGVPI
PVEGYEDKKIYVWIEAVTGYYSASKHWAEETGKDDREFWDSEAKTYYVHGKDNIPFHSVIWPAVLLGIGEGAIPRHIVSN
EYLTVEKRKLSTSKNWAVWVPDILERYDPDSIRYFLTVNAPENRDTDFSWREFIYSHNSELLGAYGNFVNRTLKFIEKYY
GGIMPKGSIDVELKDKIERLYKHVGEAIEQTKFKVALESIFDAVRFANKYFDERQPWKEREDDPVSCEETIYNCVYLIAN
FANLLEPFLPFSSERIRNTLSIVNRNWEPQHTLPTRIDSVKPLFERIDVKQIERELEKLYGAVK
>Mature_543_residues
SIFIGGAWPYANGSLHLGHIASLLPGDILARYYRAKGENVLYVSGSDCNGTPIAIRAKQEGVTAKEIANKYHEEFERCFR
NLGFTYDCYTRTDSEHHHETVQNVFLRLLEEGHIYKKTVEQAYCETCTQFLPDRYVEGVCPHCHEEARGDQCDACSAILD
PLDLLEKKCKLCGSTPSIQETEHFYFALHTFQEQIKRAVEIAKQTGTWRDNAIQLTERYVKEGLLDRAVSRDLPIGVPIP
VEGYEDKKIYVWIEAVTGYYSASKHWAEETGKDDREFWDSEAKTYYVHGKDNIPFHSVIWPAVLLGIGEGAIPRHIVSNE
YLTVEKRKLSTSKNWAVWVPDILERYDPDSIRYFLTVNAPENRDTDFSWREFIYSHNSELLGAYGNFVNRTLKFIEKYYG
GIMPKGSIDVELKDKIERLYKHVGEAIEQTKFKVALESIFDAVRFANKYFDERQPWKEREDDPVSCEETIYNCVYLIANF
ANLLEPFLPFSSERIRNTLSIVNRNWEPQHTLPTRIDSVKPLFERIDVKQIERELEKLYGAVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=563, Percent_Identity=33.5701598579041, Blast_Score=335, Evalue=6e-92,
Organism=Homo sapiens, GI24308436, Length=519, Percent_Identity=27.1676300578035, Blast_Score=162, Evalue=7e-40,
Organism=Escherichia coli, GI1788432, Length=546, Percent_Identity=30.7692307692308, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1786861, Length=139, Percent_Identity=30.2158273381295, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17540794, Length=566, Percent_Identity=31.6254416961131, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI212645096, Length=530, Percent_Identity=24.5283018867925, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71992781, Length=423, Percent_Identity=23.8770685579196, Blast_Score=99, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6321704, Length=549, Percent_Identity=33.1511839708561, Blast_Score=325, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6321610, Length=497, Percent_Identity=24.748490945674, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24655311, Length=567, Percent_Identity=34.7442680776014, Blast_Score=338, Evalue=5e-93,
Organism=Drosophila melanogaster, GI21358483, Length=519, Percent_Identity=24.6628131021195, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM1_BACAH (A0RKM3)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_897273.1
- ProteinModelPortal:   A0RKM3
- SMR:   A0RKM3
- STRING:   A0RKM3
- EnsemblBacteria:   EBBACT00000067060
- GeneID:   4542105
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_4573
- NMPDR:   fig|412694.5.peg.4400
- eggNOG:   COG0143
- GeneTree:   EBGT00070000031891
- HOGENOM:   HBG721667
- OMA:   CGNQLDP
- ProtClustDB:   PRK12268
- BioCyc:   BTHU412694:BALH_4573-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 62988; Mature: 62856

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 332-332

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIFIGGAWPYANGSLHLGHIASLLPGDILARYYRAKGENVLYVSGSDCNGTPIAIRAKQ
CEEEEECCCCCCCCCEEHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECC
EGVTAKEIANKYHEEFERCFRNLGFTYDCYTRTDSEHHHETVQNVFLRLLEEGHIYKKTV
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHH
EQAYCETCTQFLPDRYVEGVCPHCHEEARGDQCDACSAILDPLDLLEKKCKLCGSTPSIQ
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHCHHHHHHHHHHHCCCCCCCC
ETEHFYFALHTFQEQIKRAVEIAKQTGTWRDNAIQLTERYVKEGLLDRAVSRDLPIGVPI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PVEGYEDKKIYVWIEAVTGYYSASKHWAEETGKDDREFWDSEAKTYYVHGKDNIPFHSVI
CCCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEECCCCCCHHHHH
WPAVLLGIGEGAIPRHIVSNEYLTVEKRKLSTSKNWAVWVPDILERYDPDSIRYFLTVNA
HHHHHHHCCCCCCCHHHHCCCCEEEEHHHCCCCCCCEEECHHHHHCCCCCCEEEEEEEEC
PENRDTDFSWREFIYSHNSELLGAYGNFVNRTLKFIEKYYGGIMPKGSIDVELKDKIERL
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHH
YKHVGEAIEQTKFKVALESIFDAVRFANKYFDERQPWKEREDDPVSCEETIYNCVYLIAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FANLLEPFLPFSSERIRNTLSIVNRNWEPQHTLPTRIDSVKPLFERIDVKQIERELEKLY
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
GAVK
CCCC
>Mature Secondary Structure 
SIFIGGAWPYANGSLHLGHIASLLPGDILARYYRAKGENVLYVSGSDCNGTPIAIRAKQ
EEEEECCCCCCCCCEEHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECC
EGVTAKEIANKYHEEFERCFRNLGFTYDCYTRTDSEHHHETVQNVFLRLLEEGHIYKKTV
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHH
EQAYCETCTQFLPDRYVEGVCPHCHEEARGDQCDACSAILDPLDLLEKKCKLCGSTPSIQ
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHCHHHHHHHHHHHCCCCCCCC
ETEHFYFALHTFQEQIKRAVEIAKQTGTWRDNAIQLTERYVKEGLLDRAVSRDLPIGVPI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PVEGYEDKKIYVWIEAVTGYYSASKHWAEETGKDDREFWDSEAKTYYVHGKDNIPFHSVI
CCCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEECCCCCCHHHHH
WPAVLLGIGEGAIPRHIVSNEYLTVEKRKLSTSKNWAVWVPDILERYDPDSIRYFLTVNA
HHHHHHHCCCCCCCHHHHCCCCEEEEHHHCCCCCCCEEECHHHHHCCCCCCEEEEEEEEC
PENRDTDFSWREFIYSHNSELLGAYGNFVNRTLKFIEKYYGGIMPKGSIDVELKDKIERL
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHH
YKHVGEAIEQTKFKVALESIFDAVRFANKYFDERQPWKEREDDPVSCEETIYNCVYLIAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FANLLEPFLPFSSERIRNTLSIVNRNWEPQHTLPTRIDSVKPLFERIDVKQIERELEKLY
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
GAVK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA