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The map label for this gene is grepE [H]

Identifier: 118479483

GI number: 118479483

Start: 4116407

End: 4117018

Strand: Reverse

Name: grepE [H]

Synonym: BALH_3905

Alternate gene names: 118479483

Gene position: 4117018-4116407 (Counterclockwise)

Preceding gene: 118479484

Following gene: 118479482

Centisome position: 78.31

GC content: 37.91

Gene sequence:

>612_bases
TTGTGTTTAACATGGTACCTAATACCTTTTAAGGAGGTGAATATTGTGGAAGAGCGTAACGAACAAGTGGTAGAAGAAGT
AAAAGAAGCGCAAGTTGAAGAAGCTGTCACGCCAGAAAACAGTGAAGAAACTGTAGAAGAAAAAAGTGAGGCTGCTCTTT
TACAAGAAAAAGTAGATGAGTTACAAGCGAAACTAACGGAAACGGAAGGTCGCACATTACGTCTACAAGCTGATTTTGAA
AATTATAAGCGCCGTGTCCAAATGGATAAACAGGCTGCTGAAAAATATAGAGCACAAAGTCTAGTTTCAGACATTTTGCC
AGCTCTTGATAATTTTGAAAGAGCAATGCAAGTGGAAGCAACTGATGAGCAAACGAAATCCTTGTTACAAGGTATGGAAA
TGGTGCATCGTCAATTGCTAGAAGCGTTGAATAAAGAAGGTGTTGAAGTGATTGAAGCTGTTGGTAAACAGTTTGATCCT
AATGAACACCAAGCTATTATGCAAGTGGAAGACAGTGAATTTGAATCAAATGCGGTAGTTGAAGAATTCCAAAAAGGTTA
TAAACTAAAAGACCGTGTGATTCGCCCATCAATGGTAAAAGTAAATCAATAA

Upstream 100 bases:

>100_bases
GAGACAATTTACAGATGGACTTAAAAAGTAAATGAGAGTAATACATTCGCTCAAGTGATGAACTTTTCATTGCTGTGTGT
AATATATAATGCTTAGGTAA

Downstream 100 bases:

>100_bases
TTTACATAAAAGTAGGAGGATTCGCCATGAGTAAAATTATCGGTATTGACTTAGGTACAACAAACTCTTGTGTAGCTGTT
ATGGAAGGTGGAGAACCAAA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MCLTWYLIPFKEVNIVEERNEQVVEEVKEAQVEEAVTPENSEETVEEKSEAALLQEKVDELQAKLTETEGRTLRLQADFE
NYKRRVQMDKQAAEKYRAQSLVSDILPALDNFERAMQVEATDEQTKSLLQGMEMVHRQLLEALNKEGVEVIEAVGKQFDP
NEHQAIMQVEDSEFESNAVVEEFQKGYKLKDRVIRPSMVKVNQ

Sequences:

>Translated_203_residues
MCLTWYLIPFKEVNIVEERNEQVVEEVKEAQVEEAVTPENSEETVEEKSEAALLQEKVDELQAKLTETEGRTLRLQADFE
NYKRRVQMDKQAAEKYRAQSLVSDILPALDNFERAMQVEATDEQTKSLLQGMEMVHRQLLEALNKEGVEVIEAVGKQFDP
NEHQAIMQVEDSEFESNAVVEEFQKGYKLKDRVIRPSMVKVNQ
>Mature_203_residues
MCLTWYLIPFKEVNIVEERNEQVVEEVKEAQVEEAVTPENSEETVEEKSEAALLQEKVDELQAKLTETEGRTLRLQADFE
NYKRRVQMDKQAAEKYRAQSLVSDILPALDNFERAMQVEATDEQTKSLLQGMEMVHRQLLEALNKEGVEVIEAVGKQFDP
NEHQAIMQVEDSEFESNAVVEEFQKGYKLKDRVIRPSMVKVNQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=161, Percent_Identity=34.7826086956522, Blast_Score=77, Evalue=7e-15,
Organism=Escherichia coli, GI1788967, Length=173, Percent_Identity=35.8381502890173, Blast_Score=108, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324806, Length=203, Percent_Identity=30.5418719211823, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653432, Length=135, Percent_Identity=34.8148148148148, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 23478; Mature: 23478

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCLTWYLIPFKEVNIVEERNEQVVEEVKEAQVEEAVTPENSEETVEEKSEAALLQEKVDE
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LQAKLTETEGRTLRLQADFENYKRRVQMDKQAAEKYRAQSLVSDILPALDNFERAMQVEA
HHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TDEQTKSLLQGMEMVHRQLLEALNKEGVEVIEAVGKQFDPNEHQAIMQVEDSEFESNAVV
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHH
EEFQKGYKLKDRVIRPSMVKVNQ
HHHHHCCHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MCLTWYLIPFKEVNIVEERNEQVVEEVKEAQVEEAVTPENSEETVEEKSEAALLQEKVDE
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LQAKLTETEGRTLRLQADFENYKRRVQMDKQAAEKYRAQSLVSDILPALDNFERAMQVEA
HHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TDEQTKSLLQGMEMVHRQLLEALNKEGVEVIEAVGKQFDPNEHQAIMQVEDSEFESNAVV
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHH
EEFQKGYKLKDRVIRPSMVKVNQ
HHHHHCCHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA