Click here to switch to the map view.

The map label for this gene is metE [H]

Identifier: 118479431

GI number: 118479431

Start: 4059110

End: 4060942

Strand: Reverse

Name: metE [H]

Synonym: BALH_3851

Alternate gene names: 118479431

Gene position: 4060942-4059110 (Counterclockwise)

Preceding gene: 118479435

Following gene: 118479430

Centisome position: 77.25

GC content: 38.46

Gene sequence:

>1833_bases
GTGAAATTACTAGATTTATTGTCAAAAGGAATTGTAATAGGTGATGGTGCGGTTGGAACATTATTACATTCACACGGTTT
GCAAAGTAGTTTTGAAGAATTGAATATATCTGATCCAGATTTAATTATATCGATTCATAAGCAATATGTAGCTGCTGGGG
CAGATGTAATTCAAACAAATACGTACGGAGCAAATGAAGCGAAATTACGTATGTATGGTTTAGAAAATCAAGTTACTGAA
ATTAATAGAGCCGCAGTAAAACTGGCGAAAGCATCTGTTACAGACAGAAATGCAATTTTAGGAACAATAGGTGGTATGAA
ACATATCGGAGCGGTTACGACAACTGATATGGAGCGAGAATTTATGTTACTGGAACAAGCAAGTGCATTATTAGAAGAAC
AGGTTGATGGACTGCTATTAGAGACTTTTTATGATGAGTTTGAATTACTCCATGCTGTTCAGGTGTTACGAAAAGAAACG
AATATTCCAATTGTAGCGCAATTAGCATTACATGAGGCCGGTACGACTCAAAATGGAAATGATGTAAATGAAATATTAAA
ACAGCTTTTAGATTACGGTGCAAATGTTGTTGGATTGAACTGCCAACTAGGGCCACTTCATATGACGGAAGCTTTAAAAA
TGATATCGATTCCGAAAAATGGTTACTTGTCAGCGTATCCAAATGCAGGTCTCCCAAATTATGTGGAGGGACGTTATGTA
TACGAGGGGAGTCCAGCTTATTTCGAAGCGATGACACCGAAATTTATTGAGCAAGGTATTCGGTTATTGGGTGGTTGTTG
TGGCACGACTCCAGAACATATTGAAAGTATGAAACGTGCGACTCTAAATGTTACACCTGTAATAGAAAAAGATACGATTC
AAAGACCGAAAGTAGTTCATACACACGAGAAACGTTCAAAAGCTCATGTCACTTTAGCAGAGAAAGCAAAAAAACAAACG
ACAGTAGTAGTAGAATTAGATCCACCGAAAACGTTAGATACGCAGCGATTTTTTGAAGGCGCAAGAGCATTGAAAAGGGC
TGGAGCAGATGCTATTACTCTAGCAGATAATTCATTAGCATCGCCTCGTATCTCAAATATGGCAATGGGAGCATTATTAA
CGAAGCATGATATTCCGGTATTGACGCACTTAACATGTAGAGACCATAACGTCATCGGACTACAATCTCACTTACTAGGT
CTATCAGCGTTAGGTATGGAAGAAGTGTTAGCTTTAACTGGAGATCCAGCGCGTGTTGGTGATTTTCCAGGTGCCACTTC
TGTATATGATTTGTCCTCTATTGAGCTCATTAAAATGATTAAAGAAATGAATGATGGGCGCTCTATTTTAGGGAAATCGA
TAGGTCCAGCAACAAGGTTTTCAGTAGGCGGCGCGTTTAATCCGCATGTAAGACATTTAAAAGCAGCGGTTAAGCGAATG
GAACGGAAAATAGATGCTGGAGCGGAATATTTCTTAACGCAGCCGATTTATGACATCGCGTTAATCGAAGAAGTGTACGA
AGCGACGAAGCATTTGGAACAACCTATTTTTATTGGCATTATGCCATTAATAAGTAAACGGAATGCAGATTTTCTTCATT
TTGAGGTGCCAGGTATTACGCTACCGGAAGCCGTAAGGGAGAGGATGGATGGTCATGAAACGAAAGAGGCGGCAATTGAG
GAAGGAATTCGTATTTCACAAGAGTTGATTGATGAGACGATGAAATATTTTAACGGTATTTATCTTATTACGCCATTTCT
AAAATATGAAATTACAGAACATCTTGTTAAATATGTGAGAGAAAAGCAAGAAGTGAAAGAAGGTATTAATTGA

Upstream 100 bases:

>100_bases
TGCAAAACGAATGTTTTGCATAATAAGAGGAGGAACAATTATGTCCCCCTCTTCAAAGTGAAGAGGGGGTTTTTTATATT
GATAGAAATGAGGGAGATTT

Downstream 100 bases:

>100_bases
TGAAATGTATAGAAGAAAAATTACAAAATAGCATCTTACTATTAGATGGTGCAATGGGAACGATGATCCAGCAAGAGGAC
TTAACTGCGGAAGATTTCGG

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MKLLDLLSKGIVIGDGAVGTLLHSHGLQSSFEELNISDPDLIISIHKQYVAAGADVIQTNTYGANEAKLRMYGLENQVTE
INRAAVKLAKASVTDRNAILGTIGGMKHIGAVTTTDMEREFMLLEQASALLEEQVDGLLLETFYDEFELLHAVQVLRKET
NIPIVAQLALHEAGTTQNGNDVNEILKQLLDYGANVVGLNCQLGPLHMTEALKMISIPKNGYLSAYPNAGLPNYVEGRYV
YEGSPAYFEAMTPKFIEQGIRLLGGCCGTTPEHIESMKRATLNVTPVIEKDTIQRPKVVHTHEKRSKAHVTLAEKAKKQT
TVVVELDPPKTLDTQRFFEGARALKRAGADAITLADNSLASPRISNMAMGALLTKHDIPVLTHLTCRDHNVIGLQSHLLG
LSALGMEEVLALTGDPARVGDFPGATSVYDLSSIELIKMIKEMNDGRSILGKSIGPATRFSVGGAFNPHVRHLKAAVKRM
ERKIDAGAEYFLTQPIYDIALIEEVYEATKHLEQPIFIGIMPLISKRNADFLHFEVPGITLPEAVRERMDGHETKEAAIE
EGIRISQELIDETMKYFNGIYLITPFLKYEITEHLVKYVREKQEVKEGIN

Sequences:

>Translated_610_residues
MKLLDLLSKGIVIGDGAVGTLLHSHGLQSSFEELNISDPDLIISIHKQYVAAGADVIQTNTYGANEAKLRMYGLENQVTE
INRAAVKLAKASVTDRNAILGTIGGMKHIGAVTTTDMEREFMLLEQASALLEEQVDGLLLETFYDEFELLHAVQVLRKET
NIPIVAQLALHEAGTTQNGNDVNEILKQLLDYGANVVGLNCQLGPLHMTEALKMISIPKNGYLSAYPNAGLPNYVEGRYV
YEGSPAYFEAMTPKFIEQGIRLLGGCCGTTPEHIESMKRATLNVTPVIEKDTIQRPKVVHTHEKRSKAHVTLAEKAKKQT
TVVVELDPPKTLDTQRFFEGARALKRAGADAITLADNSLASPRISNMAMGALLTKHDIPVLTHLTCRDHNVIGLQSHLLG
LSALGMEEVLALTGDPARVGDFPGATSVYDLSSIELIKMIKEMNDGRSILGKSIGPATRFSVGGAFNPHVRHLKAAVKRM
ERKIDAGAEYFLTQPIYDIALIEEVYEATKHLEQPIFIGIMPLISKRNADFLHFEVPGITLPEAVRERMDGHETKEAAIE
EGIRISQELIDETMKYFNGIYLITPFLKYEITEHLVKYVREKQEVKEGIN
>Mature_610_residues
MKLLDLLSKGIVIGDGAVGTLLHSHGLQSSFEELNISDPDLIISIHKQYVAAGADVIQTNTYGANEAKLRMYGLENQVTE
INRAAVKLAKASVTDRNAILGTIGGMKHIGAVTTTDMEREFMLLEQASALLEEQVDGLLLETFYDEFELLHAVQVLRKET
NIPIVAQLALHEAGTTQNGNDVNEILKQLLDYGANVVGLNCQLGPLHMTEALKMISIPKNGYLSAYPNAGLPNYVEGRYV
YEGSPAYFEAMTPKFIEQGIRLLGGCCGTTPEHIESMKRATLNVTPVIEKDTIQRPKVVHTHEKRSKAHVTLAEKAKKQT
TVVVELDPPKTLDTQRFFEGARALKRAGADAITLADNSLASPRISNMAMGALLTKHDIPVLTHLTCRDHNVIGLQSHLLG
LSALGMEEVLALTGDPARVGDFPGATSVYDLSSIELIKMIKEMNDGRSILGKSIGPATRFSVGGAFNPHVRHLKAAVKRM
ERKIDAGAEYFLTQPIYDIALIEEVYEATKHLEQPIFIGIMPLISKRNADFLHFEVPGITLPEAVRERMDGHETKEAAIE
EGIRISQELIDETMKYFNGIYLITPFLKYEITEHLVKYVREKQEVKEGIN

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=324, Percent_Identity=27.4691358024691, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI13162290, Length=301, Percent_Identity=26.2458471760797, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI157266337, Length=307, Percent_Identity=26.3843648208469, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1790450, Length=325, Percent_Identity=28.6153846153846, Blast_Score=112, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17535405, Length=329, Percent_Identity=28.2674772036474, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25147436, Length=272, Percent_Identity=23.1617647058824, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25147441, Length=272, Percent_Identity=23.1617647058824, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 67265; Mature: 67265

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLDLLSKGIVIGDGAVGTLLHSHGLQSSFEELNISDPDLIISIHKQYVAAGADVIQTN
CCHHHHHHCCEEEECCHHHHHHHHHCCHHHHHHCCCCCCCEEEEEHHHHHHCCCCEEECC
TYGANEAKLRMYGLENQVTEINRAAVKLAKASVTDRNAILGTIGGMKHIGAVTTTDMERE
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHCCCEEECCHHHH
FMLLEQASALLEEQVDGLLLETFYDEFELLHAVQVLRKETNIPIVAQLALHEAGTTQNGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCC
DVNEILKQLLDYGANVVGLNCQLGPLHMTEALKMISIPKNGYLSAYPNAGLPNYVEGRYV
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCEEE
YEGSPAYFEAMTPKFIEQGIRLLGGCCGTTPEHIESMKRATLNVTPVIEKDTIQRPKVVH
ECCCCHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
THEKRSKAHVTLAEKAKKQTTVVVELDPPKTLDTQRFFEGARALKRAGADAITLADNSLA
CHHHCCHHEEEHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCC
SPRISNMAMGALLTKHDIPVLTHLTCRDHNVIGLQSHLLGLSALGMEEVLALTGDPARVG
CCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEHHHHHHHHHHCCHHHHHHHCCCCCCCC
DFPGATSVYDLSSIELIKMIKEMNDGRSILGKSIGPATRFSVGGAFNPHVRHLKAAVKRM
CCCCCCCEEECHHHHHHHHHHHCCCCHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHH
ERKIDAGAEYFLTQPIYDIALIEEVYEATKHLEQPIFIGIMPLISKRNADFLHFEVPGIT
HHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCEEEEECCCCC
LPEAVRERMDGHETKEAAIEEGIRISQELIDETMKYFNGIYLITPFLKYEITEHLVKYVR
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHH
EKQEVKEGIN
HHHHHHHCCC
>Mature Secondary Structure
MKLLDLLSKGIVIGDGAVGTLLHSHGLQSSFEELNISDPDLIISIHKQYVAAGADVIQTN
CCHHHHHHCCEEEECCHHHHHHHHHCCHHHHHHCCCCCCCEEEEEHHHHHHCCCCEEECC
TYGANEAKLRMYGLENQVTEINRAAVKLAKASVTDRNAILGTIGGMKHIGAVTTTDMERE
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHCCCEEECCHHHH
FMLLEQASALLEEQVDGLLLETFYDEFELLHAVQVLRKETNIPIVAQLALHEAGTTQNGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCC
DVNEILKQLLDYGANVVGLNCQLGPLHMTEALKMISIPKNGYLSAYPNAGLPNYVEGRYV
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCEEE
YEGSPAYFEAMTPKFIEQGIRLLGGCCGTTPEHIESMKRATLNVTPVIEKDTIQRPKVVH
ECCCCHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
THEKRSKAHVTLAEKAKKQTTVVVELDPPKTLDTQRFFEGARALKRAGADAITLADNSLA
CHHHCCHHEEEHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCC
SPRISNMAMGALLTKHDIPVLTHLTCRDHNVIGLQSHLLGLSALGMEEVLALTGDPARVG
CCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEHHHHHHHHHHCCHHHHHHHCCCCCCCC
DFPGATSVYDLSSIELIKMIKEMNDGRSILGKSIGPATRFSVGGAFNPHVRHLKAAVKRM
CCCCCCCEEECHHHHHHHHHHHCCCCHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHH
ERKIDAGAEYFLTQPIYDIALIEEVYEATKHLEQPIFIGIMPLISKRNADFLHFEVPGIT
HHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCEEEEECCCCC
LPEAVRERMDGHETKEAAIEEGIRISQELIDETMKYFNGIYLITPFLKYEITEHLVKYVR
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHH
EKQEVKEGIN
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]