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The map label for this gene is recN [H]

Identifier: 118479365

GI number: 118479365

Start: 3997849

End: 3999651

Strand: Reverse

Name: recN [H]

Synonym: BALH_3782

Alternate gene names: 118479365

Gene position: 3999651-3997849 (Counterclockwise)

Preceding gene: 118479366

Following gene: 118479364

Centisome position: 76.08

GC content: 35.72

Gene sequence:

>1803_bases
TTGAATAAAGAACTGAGAAAAGCCCTTAATAGGGGATTAGTTTATAACGAGGTGAATGGGGCATTGTTATCGGAATTATC
GATTAGAAACTTTGCTATTATTGAGTCATTAAATATTTCTTTTCAAAAAGGATTAACGGTTTTAAGTGGTGAAACAGGGG
CCGGAAAATCGATTATTATTGATGCGATTAGTTTACTTGTAGGTGGCCGCGGTTCGGCAGAATTTGTTCGATACGGAACA
GAAAAAGCTGAGATAGAGGGGCTATTTTATGTAGAAGATGATAAGCATCCATGTATTGAAAAGGCAGAAGAATTGGATAT
AGAAATAGAAGACGGCATGATTATTTTGAAGCGTGATATCGCTGCAAACGGAAAAAGCGTATGTCGTGTAAATGGAAAAC
TAGTTACCCTTAGTGTATTAAAAGAAATTGGAAAAACACTTGTTGATATTCATGGGCAGCACGAAACGCAAGATTTAATG
AATGAAGAACGTCATCTATTTATGCTCGATCATTTTGATGGGGAGCGTATCGTAAAACAATTGGATATATACCAAAACGT
ATACGCTGACTATGAGAAGTTAAAAAAACAATTGAAATCGTTAAGTGAAAATGAACAACAAATGGCGCATCGCTTAGATT
TAATTCAATTCCAGCATGAAGAAATCCGTAAGGCGGATTTAAAGATGGATGAAGAAAATAACTTAACTGAAGAACGATTG
CAAATTTCTAATTTCGAAAAGATTTATAAAGCATTAGGTGACGCATATCGCTCGTTAAGTGCTGATGGACAAGGATTAGA
TAATGTAAGAAGTGCAATGGGACAAATGGAGAGTATTACACATTTAGACGAAGTGTACCAAGAAAATCATGATTCAATTG
CAAATAGCTATTACCTATTAGAAGAAGTTGCATATCAACTAAGAGAAAAACTCGATATGATGGAATATGATCCAAATCGT
TTAGACGAAATTGAAACGCGTTTAAATGAAATTCGTATGCTAAAGAGAAAATATGGAAATAATGTAGAAGAGATATTAGC
GTATGCTGATAAAATTGAACAAGAGATTTTTACGATTGAAAATAAAGACGTTCATATTGAAACGACGAAGAAACAGTTGA
AGGAATTAGAAAGTGTTATTCTGAAAGAAGCAACATTGTTAAGTAATATGCGTCATGAACTTGCAGAGCATCTTACAAAT
GCCATTCATCAGGAATTAAAAGAATTGTATATGGAAAAAACAAAATTTGAAGTGAGAATCATAAAGCGAGAAGGAAATGC
GGAAGAGCCTCTCGTGGAGGGAGTACCGGTAAGGCTTACAGCGGACGGTTATGATCATGTGGAATTTTATATTTCAACCA
ATCCAGGTGAGCCGCTAAAACCACTTTCAAAGGTCGCTTCTGGTGGAGAGTTGTCCCGTATTATTTTAGCTTTAAAAAGT
ATTTTTTCTAAGCATCAAGGTGTTGCATCTGTTATTTTTGATGAAGTGGATACTGGTGTAAGTGGCCGGGTCGCACAGGC
TATTGCGGAAAAAATTTATCGAGTATCAGTAAACTCACAAGTACTTTGTATTACGCACTTACCTCAAGTAGCTTCAATGG
CGGATTCGCATTTATTTATCCGAAAACAAGTAGCGAATGATCGAACAATTACATCCGTTACCGTTTTAACTATGGAGGAT
AAAGTAACAGAAATAGCTCGAATGATTTCTGGTGTGGAAATTACAGATTTAACGACAGAACATGCGAAAGAATTACTTAC
GCAAGCGCATCATTTTAAACAGACAGCAGAGGCTATCCAGTAA

Upstream 100 bases:

>100_bases
AAAAATTAGAAAATAGGTGGATTGACCCGTAAAACAAATGTTTGGTACAATGGAGCGGTTAGAAAAGTAATTTATACATA
CAAACATATAGTATGACTAT

Downstream 100 bases:

>100_bases
TGGATAGCTTTTTTTCTCAAATGATGATTTCTTAATTCTTCCGGTTATAAATAAAGCATTGCAAGGAGAGATTAAAAAGT
GAAGCCAAGAGGCTGAATGT

Product: DNA repair protein

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MNKELRKALNRGLVYNEVNGALLSELSIRNFAIIESLNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGT
EKAEIEGLFYVEDDKHPCIEKAEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLM
NEERHLFMLDHFDGERIVKQLDIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERL
QISNFEKIYKALGDAYRSLSADGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNR
LDEIETRLNEIRMLKRKYGNNVEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTN
AIHQELKELYMEKTKFEVRIIKREGNAEEPLVEGVPVRLTADGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKS
IFSKHQGVASVIFDEVDTGVSGRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMED
KVTEIARMISGVEITDLTTEHAKELLTQAHHFKQTAEAIQ

Sequences:

>Translated_600_residues
MNKELRKALNRGLVYNEVNGALLSELSIRNFAIIESLNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGT
EKAEIEGLFYVEDDKHPCIEKAEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLM
NEERHLFMLDHFDGERIVKQLDIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERL
QISNFEKIYKALGDAYRSLSADGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNR
LDEIETRLNEIRMLKRKYGNNVEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTN
AIHQELKELYMEKTKFEVRIIKREGNAEEPLVEGVPVRLTADGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKS
IFSKHQGVASVIFDEVDTGVSGRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMED
KVTEIARMISGVEITDLTTEHAKELLTQAHHFKQTAEAIQ
>Mature_600_residues
MNKELRKALNRGLVYNEVNGALLSELSIRNFAIIESLNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGT
EKAEIEGLFYVEDDKHPCIEKAEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLM
NEERHLFMLDHFDGERIVKQLDIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERL
QISNFEKIYKALGDAYRSLSADGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNR
LDEIETRLNEIRMLKRKYGNNVEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTN
AIHQELKELYMEKTKFEVRIIKREGNAEEPLVEGVPVRLTADGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKS
IFSKHQGVASVIFDEVDTGVSGRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMED
KVTEIARMISGVEITDLTTEHAKELLTQAHHFKQTAEAIQ

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=573, Percent_Identity=32.6352530541012, Blast_Score=294, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 68187; Mature: 68187

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKELRKALNRGLVYNEVNGALLSELSIRNFAIIESLNISFQKGLTVLSGETGAGKSIII
CCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHCCCHHHHCCEEEECCCCCCCHHHH
DAISLLVGGRGSAEFVRYGTEKAEIEGLFYVEDDKHPCIEKAEELDIEIEDGMIILKRDI
HHHHHHHCCCCCHHHHHCCCCHHHCCEEEEEECCCCCHHHHHHHCCEEEECCEEEEEECC
AANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQ
CCCCCCEEEECCEEEHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCHHHHHHH
LDIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
QISNFEKIYKALGDAYRSLSADGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLL
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
EEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRKYGNNVEEILAYADKIEQEIFTIE
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEC
NKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRI
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
IKREGNAEEPLVEGVPVRLTADGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKS
EECCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHH
IFSKHQGVASVIFDEVDTGVSGRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFI
HHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHH
RKQVANDRTITSVTVLTMEDKVTEIARMISGVEITDLTTEHAKELLTQAHHFKQTAEAIQ
HHHHCCCCEEEEEEEEEEHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKELRKALNRGLVYNEVNGALLSELSIRNFAIIESLNISFQKGLTVLSGETGAGKSIII
CCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHCCCHHHHCCEEEECCCCCCCHHHH
DAISLLVGGRGSAEFVRYGTEKAEIEGLFYVEDDKHPCIEKAEELDIEIEDGMIILKRDI
HHHHHHHCCCCCHHHHHCCCCHHHCCEEEEEECCCCCHHHHHHHCCEEEECCEEEEEECC
AANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQ
CCCCCCEEEECCEEEHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCHHHHHHH
LDIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
QISNFEKIYKALGDAYRSLSADGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLL
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
EEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRKYGNNVEEILAYADKIEQEIFTIE
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEC
NKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRI
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
IKREGNAEEPLVEGVPVRLTADGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKS
EECCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHH
IFSKHQGVASVIFDEVDTGVSGRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFI
HHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHH
RKQVANDRTITSVTVLTMEDKVTEIARMISGVEITDLTTEHAKELLTQAHHFKQTAEAIQ
HHHHCCCCEEEEEEEEEEHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]