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The map label for this gene is dacF [H]

Identifier: 118479279

GI number: 118479279

Start: 3912520

End: 3913716

Strand: Reverse

Name: dacF [H]

Synonym: BALH_3693

Alternate gene names: 118479279

Gene position: 3913716-3912520 (Counterclockwise)

Preceding gene: 118479283

Following gene: 118479278

Centisome position: 74.45

GC content: 36.42

Gene sequence:

>1197_bases
TTGGTCATGAAGCGAGTTTTTGGAATACTTGTTTGTTTCATGTTATTGCTTTCTGGTACTTCAGTTAGTTTCGCACAATC
TGAGAAAACGAAGCAGGATAAAACAGAAGAAACAACGCCGAAGTTAGCAGAACAAGCGTCGTCAGCAATTGTAATTGAAC
AAGATACAGGTAAAGTTTTGTTTGATAAAAATCCAAATGAGAAATTACCGCCTGCTAGTATGACGAAGATTATGACAATG
CTATTAATTATGGAACAAGTTGAAAAAGGAAAATTAAAACTGACCGATAAAGTTAGAGCGAGTGAGCATGCAGCTTCAAT
GGGTGGATCACAAATCTTTTTAGAGCCTGGAGAAGAGATGACTGTAAATGAAATGCTAAAGGGTATTGCAATTGCATCTG
GAAATGATGCGTCTGTTGCAGTAGCGGAGCATATCGCTGGTTCAGAAGAAGGTTTTGTAAATATGATGAACAAAAAAGCG
AAAGATTTAGGGCTGAAAAATACTCATTTTCAAAATCCAACAGGTCTTCCGGCTAAAGACCATTATTCTACAGCAAATGA
TATGGCTATCATGGCGAAAGAATTGATGAAGTACCCACTTATTCGCAAATACACAGGTAAATACGAAGACTATTTACGTG
AAGATACGGATAAGAAGTTTTGGCTCGTTAATACGAATAAGTTAGTACGTTTTTATCCTGGAGTAGATGGTGTAAAAACG
GGCTTTACGACAGAAGCAAAATATTGTTTAACAGCATCGGCTGAGAAGAATGGGATGCGTGTTATTTCAGTTGTTATGGG
AGCACCTACATCAAAAGAACGGAACAATCAAGTAACGAAGCTTCTTGACTACGCATTTGGTCAATATATGACAAAGAAAT
TGTATACACGAGGCGAAAAAATTAAAACTGTCCAAGTAGGAAAAGGGAAAAAAGAAAAAGTAGATTTAGTTGCGTCAGAC
AATGTATCACTTCTTATGAAGAAGGGCGAAAATATGGACAAGGTAAAGCAAGAAGTAATTGCTGAAAAGAAAGTGAAAGC
GCCGATTAAAAAAGGTGATGCACTTGGTACGCTTGTTATTAAAAAAGATAAAGATGTTTTATTAAAACAAACAATTGTAG
CAAAAGAAGATGTTGCTGCAGCGAGCTGGTGGGAGTTATTTAAAAGAAGTTTTGGGATGTTTTCAACATCAAAATAG

Upstream 100 bases:

>100_bases
GATATAGGTGATGAATGTGTTTGTCAACTGAAAATGTTAGAAAAATATGGTATTAAAAAATAATAAATTGGAAAAAATAG
GTTTGATATAGAATGGAGGG

Downstream 100 bases:

>100_bases
CGGCAAACATTTTGCCGCTTTTCTATTCGTATGTAAGTAAATAATTGCTGAATGGTTTCACTTTATGGCGAAATAGTTCT
TCTTTTGTCAGTAGGAAGGA

Product: D-alanyl-D-alanine carboxypeptidase DacF

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MVMKRVFGILVCFMLLLSGTSVSFAQSEKTKQDKTEETTPKLAEQASSAIVIEQDTGKVLFDKNPNEKLPPASMTKIMTM
LLIMEQVEKGKLKLTDKVRASEHAASMGGSQIFLEPGEEMTVNEMLKGIAIASGNDASVAVAEHIAGSEEGFVNMMNKKA
KDLGLKNTHFQNPTGLPAKDHYSTANDMAIMAKELMKYPLIRKYTGKYEDYLREDTDKKFWLVNTNKLVRFYPGVDGVKT
GFTTEAKYCLTASAEKNGMRVISVVMGAPTSKERNNQVTKLLDYAFGQYMTKKLYTRGEKIKTVQVGKGKKEKVDLVASD
NVSLLMKKGENMDKVKQEVIAEKKVKAPIKKGDALGTLVIKKDKDVLLKQTIVAKEDVAAASWWELFKRSFGMFSTSK

Sequences:

>Translated_398_residues
MVMKRVFGILVCFMLLLSGTSVSFAQSEKTKQDKTEETTPKLAEQASSAIVIEQDTGKVLFDKNPNEKLPPASMTKIMTM
LLIMEQVEKGKLKLTDKVRASEHAASMGGSQIFLEPGEEMTVNEMLKGIAIASGNDASVAVAEHIAGSEEGFVNMMNKKA
KDLGLKNTHFQNPTGLPAKDHYSTANDMAIMAKELMKYPLIRKYTGKYEDYLREDTDKKFWLVNTNKLVRFYPGVDGVKT
GFTTEAKYCLTASAEKNGMRVISVVMGAPTSKERNNQVTKLLDYAFGQYMTKKLYTRGEKIKTVQVGKGKKEKVDLVASD
NVSLLMKKGENMDKVKQEVIAEKKVKAPIKKGDALGTLVIKKDKDVLLKQTIVAKEDVAAASWWELFKRSFGMFSTSK
>Mature_398_residues
MVMKRVFGILVCFMLLLSGTSVSFAQSEKTKQDKTEETTPKLAEQASSAIVIEQDTGKVLFDKNPNEKLPPASMTKIMTM
LLIMEQVEKGKLKLTDKVRASEHAASMGGSQIFLEPGEEMTVNEMLKGIAIASGNDASVAVAEHIAGSEEGFVNMMNKKA
KDLGLKNTHFQNPTGLPAKDHYSTANDMAIMAKELMKYPLIRKYTGKYEDYLREDTDKKFWLVNTNKLVRFYPGVDGVKT
GFTTEAKYCLTASAEKNGMRVISVVMGAPTSKERNNQVTKLLDYAFGQYMTKKLYTRGEKIKTVQVGKGKKEKVDLVASD
NVSLLMKKGENMDKVKQEVIAEKKVKAPIKKGDALGTLVIKKDKDVLLKQTIVAKEDVAAASWWELFKRSFGMFSTSK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=354, Percent_Identity=35.0282485875706, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1786851, Length=347, Percent_Identity=34.5821325648415, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87082022, Length=364, Percent_Identity=29.6703296703297, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87082054, Length=253, Percent_Identity=31.2252964426877, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44191; Mature: 44191

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMKRVFGILVCFMLLLSGTSVSFAQSEKTKQDKTEETTPKLAEQASSAIVIEQDTGKVL
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCEE
FDKNPNEKLPPASMTKIMTMLLIMEQVEKGKLKLTDKVRASEHAASMGGSQIFLEPGEEM
ECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCEEEECCCCCC
TVNEMLKGIAIASGNDASVAVAEHIAGSEEGFVNMMNKKAKDLGLKNTHFQNPTGLPAKD
CHHHHHHHEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
HYSTANDMAIMAKELMKYPLIRKYTGKYEDYLREDTDKKFWLVNTNKLVRFYPGVDGVKT
CCCCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCC
GFTTEAKYCLTASAEKNGMRVISVVMGAPTSKERNNQVTKLLDYAFGQYMTKKLYTRGEK
CCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
IKTVQVGKGKKEKVDLVASDNVSLLMKKGENMDKVKQEVIAEKKVKAPIKKGDALGTLVI
EEEEECCCCCCCCEEEEECCCCEEHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
KKDKDVLLKQTIVAKEDVAAASWWELFKRSFGMFSTSK
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVMKRVFGILVCFMLLLSGTSVSFAQSEKTKQDKTEETTPKLAEQASSAIVIEQDTGKVL
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCEE
FDKNPNEKLPPASMTKIMTMLLIMEQVEKGKLKLTDKVRASEHAASMGGSQIFLEPGEEM
ECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCEEEECCCCCC
TVNEMLKGIAIASGNDASVAVAEHIAGSEEGFVNMMNKKAKDLGLKNTHFQNPTGLPAKD
CHHHHHHHEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
HYSTANDMAIMAKELMKYPLIRKYTGKYEDYLREDTDKKFWLVNTNKLVRFYPGVDGVKT
CCCCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCC
GFTTEAKYCLTASAEKNGMRVISVVMGAPTSKERNNQVTKLLDYAFGQYMTKKLYTRGEK
CCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
IKTVQVGKGKKEKVDLVASDNVSLLMKKGENMDKVKQEVIAEKKVKAPIKKGDALGTLVI
EEEEECCCCCCCCEEEEECCCCEEHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
KKDKDVLLKQTIVAKEDVAAASWWELFKRSFGMFSTSK
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]