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The map label for this gene is def [H]

Identifier: 118479185

GI number: 118479185

Start: 3819505

End: 3820059

Strand: Direct

Name: def [H]

Synonym: BALH_3596

Alternate gene names: 118479185

Gene position: 3819505-3820059 (Clockwise)

Preceding gene: 118479180

Following gene: 118479187

Centisome position: 72.65

GC content: 36.76

Gene sequence:

>555_bases
ATGCTTACAATGAAAGATGTAATTCGCGAAGGAGATCCTATTTTGCGAAACGTTGCAGAAGAGGTAGTAATACCAGCGAG
CGAAGAAGATACAAATACCCTTAAAGAAATGATTGAATTTGTAATAAATAGCCAAGATCCTGAAATGGCTGAAAAATATA
GTTTACGCCCTGGAATCGGATTAGCGGCTCCGCAAATCGGTATTTCAAAGAAAATGATTGCAGTTCACGTAACAGATACG
GACGGTACGTTATATAGTTATGCATTATTCAATCCAAAAATCATTAGCCATTCTGTTGAACGTACATATTTACAAAGTGG
TGAAGGCTGTCTATCAGTAGACCGTGAAGTACCTGGTTATGTACCTCGTTATACAAGAATTACAGTGAAAGCAACTTCTA
TCAACGGCGAAGAAGTAAAATTACGTTTAAAAGGTTTACCAGCAATTGTATTCCAACATGAAATTGACCATTTAAATGGT
GTTATGTTCTATGACCATATTAATAAAGAAAATCCATTTGCTGCTCCTGACGGTTCAAAACCTCTGGAGCGATAA

Upstream 100 bases:

>100_bases
ACCGACCTGTAGTAAAATGCTTTCCAAAGCGCTTTCCCTTACTTGTCGATGACTTTTTCCATCTCCTTTGGTAGACTAAA
GGAGAAAGGAGAGATTACAT

Downstream 100 bases:

>100_bases
TAAAAAAAGGCGGAAGATGATTCTCCGCCTTTTTACTATTATGAAATGGGTTCCATATCCACTTATAACAATCCAGCCCA
TTTTAATCCATGATATATGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MLTMKDVIREGDPILRNVAEEVVIPASEEDTNTLKEMIEFVINSQDPEMAEKYSLRPGIGLAAPQIGISKKMIAVHVTDT
DGTLYSYALFNPKIISHSVERTYLQSGEGCLSVDREVPGYVPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNG
VMFYDHINKENPFAAPDGSKPLER

Sequences:

>Translated_184_residues
MLTMKDVIREGDPILRNVAEEVVIPASEEDTNTLKEMIEFVINSQDPEMAEKYSLRPGIGLAAPQIGISKKMIAVHVTDT
DGTLYSYALFNPKIISHSVERTYLQSGEGCLSVDREVPGYVPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNG
VMFYDHINKENPFAAPDGSKPLER
>Mature_184_residues
MLTMKDVIREGDPILRNVAEEVVIPASEEDTNTLKEMIEFVINSQDPEMAEKYSLRPGIGLAAPQIGISKKMIAVHVTDT
DGTLYSYALFNPKIISHSVERTYLQSGEGCLSVDREVPGYVPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNG
VMFYDHINKENPFAAPDGSKPLER

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=121, Percent_Identity=35.5371900826446, Blast_Score=72, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24645728, Length=174, Percent_Identity=29.3103448275862, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20542; Mature: 20542

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTMKDVIREGDPILRNVAEEVVIPASEEDTNTLKEMIEFVINSQDPEMAEKYSLRPGIG
CCCHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
LAAPQIGISKKMIAVHVTDTDGTLYSYALFNPKIISHSVERTYLQSGEGCLSVDREVPGY
CCCCCCCCCCEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEECCCCCCC
VPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNGVMFYDHINKENPFAAPDGSK
CCCEEEEEEEEEECCCCEEEEEECCCCEEEEEHHHHHCCCEEEEEECCCCCCCCCCCCCC
PLER
CCCC
>Mature Secondary Structure
MLTMKDVIREGDPILRNVAEEVVIPASEEDTNTLKEMIEFVINSQDPEMAEKYSLRPGIG
CCCHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
LAAPQIGISKKMIAVHVTDTDGTLYSYALFNPKIISHSVERTYLQSGEGCLSVDREVPGY
CCCCCCCCCCEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEECCCCCCC
VPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNGVMFYDHINKENPFAAPDGSK
CCCEEEEEEEEEECCCCEEEEEECCCCEEEEEHHHHHCCCEEEEEECCCCCCCCCCCCCC
PLER
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]