| Definition | Bacillus thuringiensis str. Al Hakam chromosome, complete genome. |
|---|---|
| Accession | NC_008600 |
| Length | 5,257,091 |
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The map label for this gene is priA [H]
Identifier: 118479090
GI number: 118479090
Start: 3713516
End: 3715921
Strand: Reverse
Name: priA [H]
Synonym: BALH_3499
Alternate gene names: 118479090
Gene position: 3715921-3713516 (Counterclockwise)
Preceding gene: 118479091
Following gene: 118479089
Centisome position: 70.68
GC content: 37.36
Gene sequence:
>2406_bases ATGAAATTTGTAAGTGTAATTGTTGATGTACCTGCACGTCAGACAGACCGACCATTTGATTATATTATCCCTAAAAAATG GGAAGATATTGTCCAGACAGGCATGCGTGTAGTAGTTCCGTTTGGCCCAAGGAAATTGCAAGGTTTTATTATTGGAATTA AAAATTCGGTTGAAGTAGAAAGTAAAAAGTTAAAGACAATCCACGAAATTTTAGATGTAACACCGGTTTTAAATGAAGAG TTGTTAAAGCTTGGGTATTGGCTTACAAGTGAAACGTTATGTTATATGATTTCAGCTTTTCAAGTTATGCTTCCGACGGC GATAAAAGCAACATATAAAAAACGTCTACAGCTTCGTAAACAAGAAGAAGTAGCACCTGAACTACTGTTTTTATTTCAGG AAAAAGAAGCGATAGATTGGGAAGCGATTGAGACGCAACCACATCTATACCGCACGATTCAACAAGAAATTAAACATGGC ACGATTGAAGTCGTTTATCAAGTAAAAGATAAAGTACAAAAGAAGAAACAAAGAGTCGTTCAACCGGAGTTGCCAGAAGA TAAATTAGAATTGGCAGCATTTGAACTGAAAAGTAAAAAACAACAAGATGTCCTCTATTATTTTGTGGAAAATTATAAAA GTGTGCCGTTGAAGGTAATAACAGAAGAGTTGCAAATAACAGATGCTCCGATTAAAGCACTTGTTAAAAAGGGGCTAATC TCAGAAAAGTATGTGGAAGTATATCGAAATCCGTATGACGATGACGATTTTGAACAAACGAAACCATTCCCACTTACTGA GGAACAAAAGCAAGTTATTACACCGATTTTATCATCAATTACAAATGAAACTTACAATCCATTTTTACTATATGGTGTTA CAGGAAGCGGAAAAACAGAAGTATATTTACAATCTATAGCAGCGGTGCTCGAGAAAGGAAAAGAAGCTATTGTACTTGTT CCTGAAATTGCACTAACGCCTCAGATGGTAGATCGTTTTAAAGGTAGATTTGGCTCGCAAGTTGCAGTTCTTCATAGTGC GCTGTCTGTTGGAGAAAAATATGATGAATGGCGTAAAATTTTAAGAAAAGAAGTGAAAGTTGTAGTTGGTGCACGTTCAG CTGTATTTGCTCCTTTTGAAAATTTAGGGATTATTATTATTGATGAGGAACATGAATCGAGCTATAAGCAAGAAGATAAT CCGAGGTATCATGCAAGGGATGTAGCTGTGTGGAGGGGACAATATCATAAATGTCCTATCGTTCTTGGTAGTGCAACACC GACGCTTGAATCGTTTGCAAGAGCGAAAAAAGGTGTGTATGAATTACTGACGATGGAAAAACGTATGAACGAACAAGCTT TACCGACAGTAGAGATTGTTGATATGCGTGAGGAACTTCGTGACGGGAATCGCTCCATGTTTTCAAAGGCACTGCATGAA AAAATAGCAGATCGATTAGAAAAAAAAGAACAAATGGTGCTCTTTTTAAATAGAAGGGGGCATTCTACATTTGTTATGTG CCGTGATTGCGGGTACGTTGTACAATGTCCGCATTGTGATATCTCACTAACATATCATAAAATGAACCATCGTTTAAAAT GCCATTATTGCAGTTACGAAGAAAATATGCCGACTGCGTGTCCTGCTTGTCAAAGTACATACATTCGTTTCTTTGGTACG GGTACGCAAAAGGTAGAAGAAGAAATTACAAAACTATTTCCAGAGGCACGAGTCATTCGAATGGATGTAGATACGACGAG TCGTAAAGGGATGCATGAGAAATTATTAAAGGCGTTCGGGGAAGAAAAAGCAGATATATTACTTGGAACACAAATGATTG CGAAAGGGTTAGATTTTCCGAAAGTAACGCTTGTTGGGGTTTTAACTGCTGATACGATGCTTCATTTACCTGATTTTAGG GCGAGTGAAAAGACTTATCAGTTATTGACGCAAGTAAGTGGACGAGCAGGCCGACATGAATTGCCAGGGGAAGTTATAAT TCAAACGTATACACCAGAACATTACAGTATAGAGTTAGCAAAGAACCAACAATACGATGTGTTTTTTGATCAAGAAATGC AAATGAGACGAACGAGACAATATCCACCTTATTACTATGTTGTACTTGTGACGGTATCTCATCCGGAATTATTGAAGGCA GTACAAGTGACGGAAAAAATTGTCGGTCATTTACGGTCGCACTGTTCGCAACAAACGATGGTGTTAGGACCGGTTGCTTC AGCAATTCCAAGGATAAAAGATAGATATCGTTATCAATGCATGATAAAATACAAACGGGAACCAAACTTAAAGAACGTGC TCAAAATGGTAAATGAACATTATCAAGCAGAAATGCAAAAAGAGCTACAAATCTCAATTGATTTTAATCCAACAATGTTA ATGTAG
Upstream 100 bases:
>100_bases GAAAAGATGGAGAAGTTATAGAGTTGCCACTTTTAACGAAGAAAGAGGTCGCTCGTGAAATATTAAAGCAAATTGAAATG ATGTTAGAAGATGATCGTCT
Downstream 100 bases:
>100_bases CGGAGGGAAATATGGCAGTTTTAGAAATAGTAAAGCATCCAAATGAAGTGTTAGAAACACCTTGTGAAAGAGTAATGAAC TTTGATAAAAAGTTAGTGAA
Product: primosome assembly protein PriA
Products: NA
Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]
Number of amino acids: Translated: 801; Mature: 801
Protein sequence:
>801_residues MKFVSVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVESKKLKTIHEILDVTPVLNEE LLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRKQEEVAPELLFLFQEKEAIDWEAIETQPHLYRTIQQEIKHG TIEVVYQVKDKVQKKKQRVVQPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTEVYLQSIAAVLEKGKEAIVLV PEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDN PRYHARDVAVWRGQYHKCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYEENMPTACPACQSTYIRFFGT GTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR ASEKTYQLLTQVSGRAGRHELPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEHYQAEMQKELQISIDFNPTML M
Sequences:
>Translated_801_residues MKFVSVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVESKKLKTIHEILDVTPVLNEE LLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRKQEEVAPELLFLFQEKEAIDWEAIETQPHLYRTIQQEIKHG TIEVVYQVKDKVQKKKQRVVQPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTEVYLQSIAAVLEKGKEAIVLV PEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDN PRYHARDVAVWRGQYHKCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYEENMPTACPACQSTYIRFFGT GTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR ASEKTYQLLTQVSGRAGRHELPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEHYQAEMQKELQISIDFNPTML M >Mature_801_residues MKFVSVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVESKKLKTIHEILDVTPVLNEE LLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRKQEEVAPELLFLFQEKEAIDWEAIETQPHLYRTIQQEIKHG TIEVVYQVKDKVQKKKQRVVQPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTEVYLQSIAAVLEKGKEAIVLV PEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDN PRYHARDVAVWRGQYHKCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYEENMPTACPACQSTYIRFFGT GTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR ASEKTYQLLTQVSGRAGRHELPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEHYQAEMQKELQISIDFNPTML M
Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase
COG id: COG1198
COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 helicase C-terminal domain [H]
Homologues:
Organism=Escherichia coli, GI1790370, Length=556, Percent_Identity=39.568345323741, Blast_Score=421, Evalue=1e-119, Organism=Escherichia coli, GI1787357, Length=191, Percent_Identity=25.130890052356, Blast_Score=65, Evalue=2e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014001 - InterPro: IPR011545 - InterPro: IPR001650 - InterPro: IPR014021 - InterPro: IPR005259 [H]
Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]
EC number: NA
Molecular weight: Translated: 92654; Mature: 92654
Theoretical pI: Translated: 8.25; Mature: 8.25
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.5 %Cys (Translated Protein) 3.1 %Met (Translated Protein) 4.6 %Cys+Met (Translated Protein) 1.5 %Cys (Mature Protein) 3.1 %Met (Mature Protein) 4.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKFVSVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVE CCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCHH SKKLKTIHEILDVTPVLNEELLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRK HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH QEEVAPELLFLFQEKEAIDWEAIETQPHLYRTIQQEIKHGTIEVVYQVKDKVQKKKQRVV HHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHCC QPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCC SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTE HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH VYLQSIAAVLEKGKEAIVLVPEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKI HHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH LRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDNPRYHARDVAVWRGQYHKCPI HHHHHHHHCCCCHHEECCHHHCCEEEEECCCCCCCCCCCCCCEEHHHHHEECCCCCCCCE VLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYE HHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEEECCCCCEEEEEECCCCCEEEEEECCC ENMPTACPACQSTYIRFFGTGTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFG CCCCCCCCHHHHCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC EEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFRASEKTYQLLTQVSGRAGRHE CCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCC LPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA CCCCEEEEEECCCCEEEEEECCCCEEEEECHHHHHHHHCCCCCEEEEEEEEECCHHHHHH VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEH HHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHH YQAEMQKELQISIDFNPTMLM HHHHHCCCEEEEEECCCCCCC >Mature Secondary Structure MKFVSVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVE CCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCHH SKKLKTIHEILDVTPVLNEELLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRK HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH QEEVAPELLFLFQEKEAIDWEAIETQPHLYRTIQQEIKHGTIEVVYQVKDKVQKKKQRVV HHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHCC QPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCC SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTE HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH VYLQSIAAVLEKGKEAIVLVPEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKI HHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH LRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDNPRYHARDVAVWRGQYHKCPI HHHHHHHHCCCCHHEECCHHHCCEEEEECCCCCCCCCCCCCCEEHHHHHEECCCCCCCCE VLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYE HHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEEECCCCCEEEEEECCCCCEEEEEECCC ENMPTACPACQSTYIRFFGTGTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFG CCCCCCCCHHHHCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC EEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFRASEKTYQLLTQVSGRAGRHE CCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCC LPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA CCCCEEEEEECCCCEEEEEECCCCEEEEECHHHHHHHHCCCCCEEEEEEEEECCHHHHHH VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEH HHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHH YQAEMQKELQISIDFNPTMLM HHHHHCCCEEEEEECCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9534248; 9384377; 9086272 [H]