| Definition | Bacillus thuringiensis str. Al Hakam chromosome, complete genome. |
|---|---|
| Accession | NC_008600 |
| Length | 5,257,091 |
Click here to switch to the map view.
The map label for this gene is dnaE [H]
Identifier: 118479038
GI number: 118479038
Start: 3656216
End: 3657667
Strand: Reverse
Name: dnaE [H]
Synonym: BALH_3446
Alternate gene names: 118479038
Gene position: 3657667-3656216 (Counterclockwise)
Preceding gene: 118479039
Following gene: 118479037
Centisome position: 69.58
GC content: 39.39
Gene sequence:
>1452_bases GTGGGGGAAAAGCTGGAGATAATATCAAATGCTTACCTATCACTATATTTCTCCGGAGAATACCAACCACGTGCCCATAA CTATACGAAAGTACTATTCGGTGAAGATTATGTTTATCGTGCAGGAACGATTGGTACAGTTGCGGAAAAAACTGCTTATG GATATGTAAAGGGTTATGCAAATGATCATAACTTAACGATTCGAAATGCAGAAATTGACCGCTTAGTAGCAGGATGTACC GGTGTAAAACGTACAACAGGACAGCATCCAGGTGGTATTATCGTTGTGCCAGATTATATGGACATTTTTGATTTCTCACC AATACAGTATCCAGCAGATTCAATAGGTGCTGAGTGGAGAACGACACACTTTGACTTCCACTCTATTCATGATAATTTAT TGAAGCTTGATATACTAGGACACGATGATCCGACAGTTATTCGTATGTTACAAGATTTAAGTGGTATTGATCCAAAAACA ATTCCAACGGATGATCCAGAAGTAATGAAAATTTTCTCAGGGCCAGAATCGTTAGGCGTAACGGAAGAACAAATTAACTG TAAAACAGGTACACTTGGTATACCAGAGTTTGGTACGAAATTCGTAAGACAGATGTTAGAAGAGACGAAACCGACGACAT TCTCAGAGTTAGTCCAAATTTCTGGATTATCACATGGTACGGACGTATGGCTTGGTAATGCAAACGAGTTAATTTATAAC GGTACATGTACGCTGAGTGAAGTTATCGGTTGTCGTGATGACATCATGGTATATTTAATCTATCAAGGTTTAGACCCATC ATTAGCCTTCAAAATCATGGAGTCAGTACGTAAAGGTAAAGGTGTACCAGAAGAATGGGAAGAGGACATGAAAAGTAATA ATGTACCAGGTTGGTATATTGATTCATGTAAGAAGATTAAGTACATGTTCCCGAAAGCCCATGCGGCTGCTTACGTACTT ATGGCCGTGCGTATCGCATACTTTAAAGTGCATTTCGCGCTTTTATTCTATGCGGCATACTTTACGGTTCGTGCAGATGA CTTTGACGTAGAAGCAATGGCAAAAGGTTCAGCATCTATACGTGCAAGAATTGATGAAATTGCGCAGAAAGGTTTAGATG CAGCACCGAAAGAGAAGAGTTTATTGACTGTACTAGAAATGACACTTGAAATGTGTGAGCGTGGATACTCATTCCAAAAG GTGGATTTATATCGCTCACATGCAACAGACTTTATTATTGATGGAGACTCATTAATCCCTCCATTTAATGCGGTGCCTGG TCTTGGTACAAACGCAGCTTTAAGTATTGTGGAAGCACGTAAAAATGGAGAATTCTTATCAAAAGAAGACTTGCAGCAGC GTAGTAAGGTTTCGAAAACAATTATTGAGTATTTAGATAGTCAAGGTTGTTTAGGTGATTTGCCGGATCAAAACCAGTTA TCGCTGTTCTGA
Upstream 100 bases:
>100_bases TTGAGAACGGAAGTAGGAAATCCAAAACTCAGCTTATAAACGTCGACAAAGTTAACGATGGAACGCCGTGTGAGTGTGAA AGCCTCATGCACGGTGTGGA
Downstream 100 bases:
>100_bases TAGGTAGGAAAAGTCTTTGCAAATCAACATTTGGTATGGTATACTATTAACCGAGATAACCATGTCATGTATATTGTGTG AAAAGAAGAGTGGGGAAACC
Product: DNA polymerase III, subunit alpha
Products: NA
Alternate protein names: PolIII [H]
Number of amino acids: Translated: 483; Mature: 482
Protein sequence:
>483_residues MGEKLEIISNAYLSLYFSGEYQPRAHNYTKVLFGEDYVYRAGTIGTVAEKTAYGYVKGYANDHNLTIRNAEIDRLVAGCT GVKRTTGQHPGGIIVVPDYMDIFDFSPIQYPADSIGAEWRTTHFDFHSIHDNLLKLDILGHDDPTVIRMLQDLSGIDPKT IPTDDPEVMKIFSGPESLGVTEEQINCKTGTLGIPEFGTKFVRQMLEETKPTTFSELVQISGLSHGTDVWLGNANELIYN GTCTLSEVIGCRDDIMVYLIYQGLDPSLAFKIMESVRKGKGVPEEWEEDMKSNNVPGWYIDSCKKIKYMFPKAHAAAYVL MAVRIAYFKVHFALLFYAAYFTVRADDFDVEAMAKGSASIRARIDEIAQKGLDAAPKEKSLLTVLEMTLEMCERGYSFQK VDLYRSHATDFIIDGDSLIPPFNAVPGLGTNAALSIVEARKNGEFLSKEDLQQRSKVSKTIIEYLDSQGCLGDLPDQNQL SLF
Sequences:
>Translated_483_residues MGEKLEIISNAYLSLYFSGEYQPRAHNYTKVLFGEDYVYRAGTIGTVAEKTAYGYVKGYANDHNLTIRNAEIDRLVAGCT GVKRTTGQHPGGIIVVPDYMDIFDFSPIQYPADSIGAEWRTTHFDFHSIHDNLLKLDILGHDDPTVIRMLQDLSGIDPKT IPTDDPEVMKIFSGPESLGVTEEQINCKTGTLGIPEFGTKFVRQMLEETKPTTFSELVQISGLSHGTDVWLGNANELIYN GTCTLSEVIGCRDDIMVYLIYQGLDPSLAFKIMESVRKGKGVPEEWEEDMKSNNVPGWYIDSCKKIKYMFPKAHAAAYVL MAVRIAYFKVHFALLFYAAYFTVRADDFDVEAMAKGSASIRARIDEIAQKGLDAAPKEKSLLTVLEMTLEMCERGYSFQK VDLYRSHATDFIIDGDSLIPPFNAVPGLGTNAALSIVEARKNGEFLSKEDLQQRSKVSKTIIEYLDSQGCLGDLPDQNQL SLF >Mature_482_residues GEKLEIISNAYLSLYFSGEYQPRAHNYTKVLFGEDYVYRAGTIGTVAEKTAYGYVKGYANDHNLTIRNAEIDRLVAGCTG VKRTTGQHPGGIIVVPDYMDIFDFSPIQYPADSIGAEWRTTHFDFHSIHDNLLKLDILGHDDPTVIRMLQDLSGIDPKTI PTDDPEVMKIFSGPESLGVTEEQINCKTGTLGIPEFGTKFVRQMLEETKPTTFSELVQISGLSHGTDVWLGNANELIYNG TCTLSEVIGCRDDIMVYLIYQGLDPSLAFKIMESVRKGKGVPEEWEEDMKSNNVPGWYIDSCKKIKYMFPKAHAAAYVLM AVRIAYFKVHFALLFYAAYFTVRADDFDVEAMAKGSASIRARIDEIAQKGLDAAPKEKSLLTVLEMTLEMCERGYSFQKV DLYRSHATDFIIDGDSLIPPFNAVPGLGTNAALSIVEARKNGEFLSKEDLQQRSKVSKTIIEYLDSQGCLGDLPDQNQLS LF
Specific function: Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]
COG id: COG2176
COG function: function code L; DNA polymerase III, alpha subunit (gram-positive type)
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 exonuclease domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR011708 - InterPro: IPR006054 - InterPro: IPR006055 - InterPro: IPR013520 - InterPro: IPR016027 - InterPro: IPR004365 - InterPro: IPR004013 - InterPro: IPR003141 - InterPro: IPR006308 - InterPro: IPR012337 [H]
Pfam domain/function: PF07733 DNA_pol3_alpha; PF00929 Exonuc_X-T; PF02811 PHP; PF01336 tRNA_anti [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 53909; Mature: 53778
Theoretical pI: Translated: 4.80; Mature: 4.80
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.4 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 4.1 %Cys+Met (Translated Protein) 1.5 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 3.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGEKLEIISNAYLSLYFSGEYQPRAHNYTKVLFGEDYVYRAGTIGTVAEKTAYGYVKGYA CCCHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCEEEECCCCCHHHHHHHHHEEEECC NDHNLTIRNAEIDRLVAGCTGVKRTTGQHPGGIIVVPDYMDIFDFSPIQYPADSIGAEWR CCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHCCCCCCCCHHHCCCCCE TTHFDFHSIHDNLLKLDILGHDDPTVIRMLQDLSGIDPKTIPTDDPEVMKIFSGPESLGV EEECCHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHCCC TEEQINCKTGTLGIPEFGTKFVRQMLEETKPTTFSELVQISGLSHGTDVWLGNANELIYN CHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEECCCCEEEEE GTCTLSEVIGCRDDIMVYLIYQGLDPSLAFKIMESVRKGKGVPEEWEEDMKSNNVPGWYI CCCHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEC DSCKKIKYMFPKAHAAAYVLMAVRIAYFKVHFALLFYAAYFTVRADDFDVEAMAKGSASI CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHCCCHHH RARIDEIAQKGLDAAPKEKSLLTVLEMTLEMCERGYSFQKVDLYRSHATDFIIDGDSLIP HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCEEEECCCCCCC PFNAVPGLGTNAALSIVEARKNGEFLSKEDLQQRSKVSKTIIEYLDSQGCLGDLPDQNQL CCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC SLF CCC >Mature Secondary Structure GEKLEIISNAYLSLYFSGEYQPRAHNYTKVLFGEDYVYRAGTIGTVAEKTAYGYVKGYA CCHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCEEEECCCCCHHHHHHHHHEEEECC NDHNLTIRNAEIDRLVAGCTGVKRTTGQHPGGIIVVPDYMDIFDFSPIQYPADSIGAEWR CCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHCCCCCCCCHHHCCCCCE TTHFDFHSIHDNLLKLDILGHDDPTVIRMLQDLSGIDPKTIPTDDPEVMKIFSGPESLGV EEECCHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHCCC TEEQINCKTGTLGIPEFGTKFVRQMLEETKPTTFSELVQISGLSHGTDVWLGNANELIYN CHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEECCCCEEEEE GTCTLSEVIGCRDDIMVYLIYQGLDPSLAFKIMESVRKGKGVPEEWEEDMKSNNVPGWYI CCCHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEC DSCKKIKYMFPKAHAAAYVLMAVRIAYFKVHFALLFYAAYFTVRADDFDVEAMAKGSASI CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHCCCHHH RARIDEIAQKGLDAAPKEKSLLTVLEMTLEMCERGYSFQKVDLYRSHATDFIIDGDSLIP HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCEEEECCCCCCC PFNAVPGLGTNAALSIVEARKNGEFLSKEDLQQRSKVSKTIIEYLDSQGCLGDLPDQNQL CCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC SLF CCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12721630 [H]