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The map label for this gene is truB

Identifier: 118479030

GI number: 118479030

Start: 3649968

End: 3650891

Strand: Reverse

Name: truB

Synonym: BALH_3438

Alternate gene names: 118479030

Gene position: 3650891-3649968 (Counterclockwise)

Preceding gene: 118479031

Following gene: 118479029

Centisome position: 69.45

GC content: 36.36

Gene sequence:

>924_bases
ATGGAAGGTGTAGTATTATTACATAAGCCAAAAGGCATGACATCACATGATTGTGTATTTAAATTAAGAAAGATATTGCG
TGAAAAACGAATTGGTCATACAGGAACATTAGATCCAGATGTAACAGGAGTATTGCCGATTTGTGTTGGGAGAGCAACGA
AGATTGCACAATTTTTAACGAGTGAGACGAAAACATATGAAGGTGAAGTAACACTTGGATTTTCAACAACAACTGAAGAT
GCTTCTGGTGAAGTTGTGGAAACGAAACATGTAGATCGTGTTATTACTCGTAAGGAAGTAGAAGGGGCTCTTGCTGCATT
AACAGGTACGATTGAACAAATGCCTCCGATGTTTTCAGCTGTAAAAGTTAACGGGAAAAAATTATATGAATATGCAAGAG
CAGGACAAGAAGTTGAACGTCCAGTTCGTACAATTACAATTCATGAATTTGTATTACTAGATGACCGTGAAGTGTTTGAG
GGAGAAACTATTTCATTCCGTTTCCGTGTAACGTGTAGTAAAGGAACATATGTAAGAACGTTAGCCGTAATGATTGGGGA
AAAACTCGGTTTTCCATCACATATGTCTCACCTTGTAAGAACAGCTTCTGGTGAATTTTTATTAGAGGATTGCATATCAT
TTGAAGAAATTGAGGAAAACGTGCAAAATGGAACAGTAGAGTCTATCTTCATTTCAATTGATGAAGCATTAAGTAAATTC
CCGAAAATGGTTGTAGATGAAAAGCAAGCAGAAAAAATAAAGAATGGTATGTTCTTAAAAAATGAATTGCAAATAACGGC
GCCATTTATAACCGTGTTTGATAAAAACGATCGCTGCCTTGCAATTTATGAGCACCATCCAAAGCACCCTGGTATGCTAA
AGCCGATGAAAGTACTTGTGAATAATCAAGAACTAAAGCTATAA

Upstream 100 bases:

>100_bases
AACGTGAAGAATAATGGATAGACATTTGTCTATCCATTTTTTCAATGTAAAATAAGTTTTAGGTAATGATTATGAAAGAA
TTACGATGAGGTGAACATAT

Downstream 100 bases:

>100_bases
TGAATTATTGGAAGGATTATTACAGAAAAAGGTGAATTCAAGCGTGAAACTTATTCATTTAACTCATCCACATGAACAAA
ATAAATTAGAATTACCACCT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MEGVVLLHKPKGMTSHDCVFKLRKILREKRIGHTGTLDPDVTGVLPICVGRATKIAQFLTSETKTYEGEVTLGFSTTTED
ASGEVVETKHVDRVITRKEVEGALAALTGTIEQMPPMFSAVKVNGKKLYEYARAGQEVERPVRTITIHEFVLLDDREVFE
GETISFRFRVTCSKGTYVRTLAVMIGEKLGFPSHMSHLVRTASGEFLLEDCISFEEIEENVQNGTVESIFISIDEALSKF
PKMVVDEKQAEKIKNGMFLKNELQITAPFITVFDKNDRCLAIYEHHPKHPGMLKPMKVLVNNQELKL

Sequences:

>Translated_307_residues
MEGVVLLHKPKGMTSHDCVFKLRKILREKRIGHTGTLDPDVTGVLPICVGRATKIAQFLTSETKTYEGEVTLGFSTTTED
ASGEVVETKHVDRVITRKEVEGALAALTGTIEQMPPMFSAVKVNGKKLYEYARAGQEVERPVRTITIHEFVLLDDREVFE
GETISFRFRVTCSKGTYVRTLAVMIGEKLGFPSHMSHLVRTASGEFLLEDCISFEEIEENVQNGTVESIFISIDEALSKF
PKMVVDEKQAEKIKNGMFLKNELQITAPFITVFDKNDRCLAIYEHHPKHPGMLKPMKVLVNNQELKL
>Mature_307_residues
MEGVVLLHKPKGMTSHDCVFKLRKILREKRIGHTGTLDPDVTGVLPICVGRATKIAQFLTSETKTYEGEVTLGFSTTTED
ASGEVVETKHVDRVITRKEVEGALAALTGTIEQMPPMFSAVKVNGKKLYEYARAGQEVERPVRTITIHEFVLLDDREVFE
GETISFRFRVTCSKGTYVRTLAVMIGEKLGFPSHMSHLVRTASGEFLLEDCISFEEIEENVQNGTVESIFISIDEALSKF
PKMVVDEKQAEKIKNGMFLKNELQITAPFITVFDKNDRCLAIYEHHPKHPGMLKPMKVLVNNQELKL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=258, Percent_Identity=33.7209302325581, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI4503337, Length=202, Percent_Identity=33.6633663366337, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI215599015, Length=202, Percent_Identity=33.6633663366337, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI2367200, Length=265, Percent_Identity=37.7358490566038, Blast_Score=183, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17553978, Length=202, Percent_Identity=30.6930693069307, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=265, Percent_Identity=29.0566037735849, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324037, Length=138, Percent_Identity=37.6811594202899, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281364189, Length=202, Percent_Identity=32.1782178217822, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364187, Length=202, Percent_Identity=32.1782178217822, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364185, Length=202, Percent_Identity=32.1782178217822, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364183, Length=202, Percent_Identity=32.1782178217822, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI62471759, Length=202, Percent_Identity=32.1782178217822, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17975520, Length=202, Percent_Identity=32.1782178217822, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_BACAH (A0RHI1)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_896181.1
- ProteinModelPortal:   A0RHI1
- SMR:   A0RHI1
- STRING:   A0RHI1
- EnsemblBacteria:   EBBACT00000070527
- GeneID:   4544769
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_3438
- eggNOG:   COG0130
- GeneTree:   EBGT00050000002629
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK01550
- BioCyc:   BTHU412694:BALH_3438-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 34502; Mature: 34502

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGVVLLHKPKGMTSHDCVFKLRKILREKRIGHTGTLDPDVTGVLPICVGRATKIAQFLT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHH
SETKTYEGEVTLGFSTTTEDASGEVVETKHVDRVITRKEVEGALAALTGTIEQMPPMFSA
HCCCEECCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VKVNGKKLYEYARAGQEVERPVRTITIHEFVLLDDREVFEGETISFRFRVTCSKGTYVRT
EEECHHHHHHHHHCCHHHHCCHHEEEEEEEEEECCCHHHCCCEEEEEEEEEECCCCHHHH
LAVMIGEKLGFPSHMSHLVRTASGEFLLEDCISFEEIEENVQNGTVESIFISIDEALSKF
HHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHCCCHHHEEEEHHHHHHHH
PKMVVDEKQAEKIKNGMFLKNELQITAPFITVFDKNDRCLAIYEHHPKHPGMLKPMKVLV
HHHHHCHHHHHHHHCCCEEECCEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHC
NNQELKL
CCCCCCC
>Mature Secondary Structure
MEGVVLLHKPKGMTSHDCVFKLRKILREKRIGHTGTLDPDVTGVLPICVGRATKIAQFLT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHH
SETKTYEGEVTLGFSTTTEDASGEVVETKHVDRVITRKEVEGALAALTGTIEQMPPMFSA
HCCCEECCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VKVNGKKLYEYARAGQEVERPVRTITIHEFVLLDDREVFEGETISFRFRVTCSKGTYVRT
EEECHHHHHHHHHCCHHHHCCHHEEEEEEEEEECCCHHHCCCEEEEEEEEEECCCCHHHH
LAVMIGEKLGFPSHMSHLVRTASGEFLLEDCISFEEIEENVQNGTVESIFISIDEALSKF
HHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHCCCHHHEEEEHHHHHHHH
PKMVVDEKQAEKIKNGMFLKNELQITAPFITVFDKNDRCLAIYEHHPKHPGMLKPMKVLV
HHHHHCHHHHHHHHCCCEEECCEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHC
NNQELKL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA