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The map label for this gene is cwlH [H]

Identifier: 118478910

GI number: 118478910

Start: 3512535

End: 3514319

Strand: Reverse

Name: cwlH [H]

Synonym: BALH_3313

Alternate gene names: 118478910

Gene position: 3514319-3512535 (Counterclockwise)

Preceding gene: 118478911

Following gene: 118478909

Centisome position: 66.85

GC content: 36.58

Gene sequence:

>1785_bases
TTGGCTGAGGAGGGGAAATTTAACACGAATTTAATGATATGCGTGTTAAGTAGTATGATGAAAAAAGGATTTTATAATGT
TTTAGCTGCATCAATTGTATTTAGTATGGCAACGATTCCAAATTATTCCTATGCTAACGAACTTGACAAAACGGTTAAAG
TTTCACCTGATGAACAAGCTTTAAAAAGTATTGAAAACCATATGAAAGATGAAGATGGTAGAGGGGAAGATAAAGGAGTA
AGAAATGAAGTGCAAGGTGAATTTCTTGTACATATAGTGAAAGAGGTACCTTTATACGATTCTAGTAATTTTCAAAAAGA
GACAGGGGTTCGTATTTCGAATCAAGTCGTGAAAGCTGAGAAGAGAAAAGGAAACGCTTACTATGTTCAAACTTCATCGG
GAACTGGTTGGATACAAAATAGCGATGGGAATGTAGAAGTTAAAGAAATTCACCCGTTATTAAGTGAGAAGTTAATTGTA
AATGAAGAAACCTCTACATACTCTGAACCATTTGCTTCATACAAAGAAGAAAATGTACTGGAGCCACAAACTATTCAAGC
AATTGGACAGGCTGGAGAATGGTTCCAAGTAAAAATAAATAATGAGATGAAATGGATTCATTCACCTTCAGCAAAATTTG
AAGGAACAAAAGCTTCGCTTATACAAGGCGCAGCACCAATACGTACAAAATATGCAGCAGCTATGTATGCTGCACCAATA
GAAGAGAAGACAACGGATATTTATGGTGTACCTTTAAAAGAAATGATTGTACCGAAAGGAAATGAAAATATTCGTCCGGG
TTATGCGATGAACCCGAAGTATATTACGATTCATGAGACAGATAATTACAGTGTGGGGGCAAATGCTAGAAACCATGCGA
TATATTTATATAACCAAGCAACGGGAACGGAAGATCGTTCAGCGTCGTGGCATTTTACAGTAGATGATAAAGAGATTTAT
CAACACCTGCCATTAAACGAAAATGCTTGGCATGCAGGAGATGGGGCAGAAGGAACTGGAAATCGAGAATCAATTGCGAT
TGAAATTGCTGTAAACGAAGATGGTGATTATAATAAAGCCGTTGAAAATGCTCGTAAACTAGCAGCTTATTTAATGGGGG
AATTAAATATTCCTTTAGAGAATGTAAAAAAACATCAATTTTGGAGTGGGAAGATTTGTCCAGCCATTATGATTAAAAAT
AATGGCTGGGAACCATTCTTACAAGGAACAAAAGTGTATTATGATGCGAACCATAAAGATGATATAACGGGTGGTTGGTA
CGAAGCAGACATTCGTGAATTAGATAAGCGCGGCATTATGGTTGGAGATGGGAAAGGTTCTTATTGGCCAGAGCGTCTTG
TGACACGTGGTGAATTTGCGAACTTCATTTCAAGATCATTGCAGTTACCTGAAGGATCATCGAATTTTAGTGACTTAAAT
GCAGCACATCCATCGCTAATAGATGGTATTAATCGTGCAGCAAGTGCCGGGATAATAAGTGGTAGAGGAAATGGGATATT
TGCTCCAAATGATACGATTAAACGAGATGAAGTTGTTATCATGATTGACCGTGCACTGCAATATAAAAAAATTAAAGGTA
ACTTAGTTCCACTGCCATTCTCGGATCAAGATTTAGCATATGATAAACAAGCTGTACAACGTGTTTATGGATTAGGCATT
GTAAAAGGAAATGAAAATAATGAATTTATGCCGAAAGGTTCAGCAACGCGTGGAGAATCAGCAGCATTTATTAATAGAAT
GTTAGAAGTAATAGAAAGTAATTAA

Upstream 100 bases:

>100_bases
TGTAGGCAAATAGTAGTCATTCCTATCTATACGAATTGGAATGAGTCTGTTATATTGTAAATGGTTATGTTGTTTAATAG
TTTAGTAGATAACCTATATT

Downstream 100 bases:

>100_bases
TAAAAAAGCATTCGCTTTTGCGAGTGCTTTTTTATTTTTCAATATGTCACGGAAGGGAGTGGAGGTTTTTGAGTGGAATA
AGGAATGAAAGTGGAGGTGA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MAEEGKFNTNLMICVLSSMMKKGFYNVLAASIVFSMATIPNYSYANELDKTVKVSPDEQALKSIENHMKDEDGRGEDKGV
RNEVQGEFLVHIVKEVPLYDSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLIV
NEETSTYSEPFASYKEENVLEPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPI
EEKTTDIYGVPLKEMIVPKGNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEIY
QHLPLNENAWHAGDGAEGTGNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPLENVKKHQFWSGKICPAIMIKN
NGWEPFLQGTKVYYDANHKDDITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLN
AAHPSLIDGINRAASAGIISGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLGI
VKGNENNEFMPKGSATRGESAAFINRMLEVIESN

Sequences:

>Translated_594_residues
MAEEGKFNTNLMICVLSSMMKKGFYNVLAASIVFSMATIPNYSYANELDKTVKVSPDEQALKSIENHMKDEDGRGEDKGV
RNEVQGEFLVHIVKEVPLYDSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLIV
NEETSTYSEPFASYKEENVLEPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPI
EEKTTDIYGVPLKEMIVPKGNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEIY
QHLPLNENAWHAGDGAEGTGNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPLENVKKHQFWSGKICPAIMIKN
NGWEPFLQGTKVYYDANHKDDITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLN
AAHPSLIDGINRAASAGIISGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLGI
VKGNENNEFMPKGSATRGESAAFINRMLEVIESN
>Mature_593_residues
AEEGKFNTNLMICVLSSMMKKGFYNVLAASIVFSMATIPNYSYANELDKTVKVSPDEQALKSIENHMKDEDGRGEDKGVR
NEVQGEFLVHIVKEVPLYDSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLIVN
EETSTYSEPFASYKEENVLEPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIE
EKTTDIYGVPLKEMIVPKGNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEIYQ
HLPLNENAWHAGDGAEGTGNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPLENVKKHQFWSGKICPAIMIKNN
GWEPFLQGTKVYYDANHKDDITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLNA
AHPSLIDGINRAASAGIISGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLGIV
KGNENNEFMPKGSATRGESAAFINRMLEVIESN

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 66157; Mature: 66026

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEGKFNTNLMICVLSSMMKKGFYNVLAASIVFSMATIPNYSYANELDKTVKVSPDEQA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEECCCHHHH
LKSIENHMKDEDGRGEDKGVRNEVQGEFLVHIVKEVPLYDSSNFQKETGVRISNQVVKAE
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHH
KRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLIVNEETSTYSEPFASYKEENVL
HCCCCEEEEEECCCCCEEECCCCCEEHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCC
EPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPI
CHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCC
EEKTTDIYGVPLKEMIVPKGNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQA
CCCCCCEECCCHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCEEEEEECC
TGTEDRSASWHFTVDDKEIYQHLPLNENAWHAGDGAEGTGNRESIAIEIAVNEDGDYNKA
CCCCCCCCEEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHH
VENARKLAAYLMGELNIPLENVKKHQFWSGKICPAIMIKNNGWEPFLQGTKVYYDANHKD
HHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCHHHCCCEEEECCCCCC
DITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLN
CCCCCEEHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AAHPSLIDGINRAASAGIISGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPF
CCCCHHHHHHHHHHHCCEEECCCCEEECCCCCCCCCCEEEEECCCHHHHHHCCCEEECCC
SDQDLAYDKQAVQRVYGLGIVKGNENNEFMPKGSATRGESAAFINRMLEVIESN
CCCCCHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEEGKFNTNLMICVLSSMMKKGFYNVLAASIVFSMATIPNYSYANELDKTVKVSPDEQA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEECCCHHHH
LKSIENHMKDEDGRGEDKGVRNEVQGEFLVHIVKEVPLYDSSNFQKETGVRISNQVVKAE
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHH
KRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLIVNEETSTYSEPFASYKEENVL
HCCCCEEEEEECCCCCEEECCCCCEEHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCC
EPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPI
CHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCC
EEKTTDIYGVPLKEMIVPKGNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQA
CCCCCCEECCCHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCEEEEEECC
TGTEDRSASWHFTVDDKEIYQHLPLNENAWHAGDGAEGTGNRESIAIEIAVNEDGDYNKA
CCCCCCCCEEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHH
VENARKLAAYLMGELNIPLENVKKHQFWSGKICPAIMIKNNGWEPFLQGTKVYYDANHKD
HHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCHHHCCCEEEECCCCCC
DITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLN
CCCCCEEHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AAHPSLIDGINRAASAGIISGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPF
CCCCHHHHHHHHHHHCCEEECCCCEEECCCCCCCCCCEEEEECCCHHHHHHCCCEEECCC
SDQDLAYDKQAVQRVYGLGIVKGNENNEFMPKGSATRGESAAFINRMLEVIESN
CCCCCHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]