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The map label for this gene is fadD [H]

Identifier: 118478865

GI number: 118478865

Start: 3460677

End: 3462338

Strand: Reverse

Name: fadD [H]

Synonym: BALH_3263

Alternate gene names: 118478865

Gene position: 3462338-3460677 (Counterclockwise)

Preceding gene: 118478866

Following gene: 118478864

Centisome position: 65.86

GC content: 34.6

Gene sequence:

>1662_bases
GTGGGAGACGATGAAAGTGAGTGCTGTGTTTTTACCAGGGGATTGCATAAAAGCTATTATTGCAGCGATTCTTGTAACGA
AATTACATCGTTCATTGAAACATATTATTACGCCTGCTCTAAAGAATGGGAAATATACAAATGCGGGATAAGAAAGAGAA
GAAACACGTTTATTGCGTGTTTCTTTTTAGTATTTTGTAAAGAAAGGACATCTCATATGGGAATAACAAAAGAATATAAA
AAACATGCCTCTTTACAACCGAATAAAATAGCAATAAAGGAAAATGATCGAGTTTTAACATATAAAGAATGGTTTGAGTC
AGTGTATAAAGTAGCAAACTGGTTGAATAAAAAAGAATCGAAGAATAAAACGATAGCGATTGTATTAGAAAATCGTATAG
AGTTTTTACAACTATTCGCTGGTGCTGCTATGACCGGATGGGTTTGTGTGCCACTAGATATAAAGTGGAAACAAGATGAG
CTCAAAGAAAGAATTGCAATTAGTAATCCGGATATGATTGTGAAAGAGAGATATAAGTTAAATGATTTACCGGATGAAGA
AGGAAGAGTAATTGAAATTGATGAGTGGAAAAGGATGATTGAGAAATATCTTCCTACATATTTCCCTATAGAGAATGTAC
AAAATGCTCCTTTTTATATGGGATTCACATCAGGATCGACTGGGAAAGCAAAAGCGTTTTTACGTGCGCAACAATCGTGG
CTTCATAGTTTTGATTGTAATGTACATGACTTTCATATGAAAAGAGAGGATTCGATTTTAATAGCTGGGACGCTTGTTCA
TTCTCTTTTCTTATACGGGGCAATAAGCGCATTATATGTAGGGCAAACGGTTCACATTATGAGAAAGTTCATTCCAAATC
AAGTATTAGATAAGTTAGAAACTGAAAATATATCAGTTATGTATACAGTTCCGACAATGCTTGAAGCTTTATATAAAGAA
AATAGAGTGATAGAAAATAAAATGAAAATTATTTCGTCAGGGGCGAAATGGGAAGCTGGAGCGAAAGAAAAAATAAAGAA
TATATTTCCTTATGCGAAAAGATATGAATTTTATGGTGCATCGGAACTGAGTTTTGTAACAGCATTAGTTGATGAAGAGA
GCGAAAGAAGGCCAAATTCAGTAGGGAAACCTTGTCACAATGTGCAAGTTCGAGTATGTAATGAGGCAGGAAAAGAAGTA
CAGAAAGGTGAGATAGGAACTGTTTATGTGAAAAGTGATCAGTTTTTTATGGGATACATAATAGATGGGGTTTTAGCACG
GGAGTTGAATGCAGATGGCTGGATGACAGTGCGAGATGTAGGTTATGAAGATGAAGAAGGATTTATTTATATTGTCGGTA
GAGAGAAGAATATGATTTTATTTGGAGGAATTAATATTTTCCCAGAAGAAATAGAAAGCGTATTACATGAACATCCAGCT
GTTGATGAAATAGTTGTAATTGGTGTGGAAGATAGTTACTGGGGTGAAAAACCTGTCGCGATTGTAAAAGGAAGTGCTAC
GAGGCAACAATTAAAGAGTTTTTGCTTACAAAGATTATCCTCTTTTAAAATACCGAAAGAATGGTATTTTGTAGATGAAA
TACCGTATACAAATAGCGGGAAAATCGCTCGTATGGAAGCAAAAAGTATCATTGAAAATTAG

Upstream 100 bases:

>100_bases
TTTCTATTATAAAAGTATTATGCATTAATATAATTGGTGGTATTTTCGTAGTTTATACATTTGGTATTACTGTACAAGCT
TTCTTAATGGGTGTTTCCGT

Downstream 100 bases:

>100_bases
GAGAAAATATATGAATAGAGCAGTTATTGTAGAAGCGAAAAGGACACCTATTGGTAAGAAGAATGGGATGTTAAAAGACT
ATGAAGTTCAGCAATTAGCA

Product: acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MGDDESECCVFTRGLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIACFFLVFCKERTSHMGITKEYK
KHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNKKESKNKTIAIVLENRIEFLQLFAGAAMTGWVCVPLDIKWKQDE
LKERIAISNPDMIVKERYKLNDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDKLETENISVMYTVPTMLEALYKE
NRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEV
QKGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPA
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKSFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIEN

Sequences:

>Translated_553_residues
MGDDESECCVFTRGLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIACFFLVFCKERTSHMGITKEYK
KHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNKKESKNKTIAIVLENRIEFLQLFAGAAMTGWVCVPLDIKWKQDE
LKERIAISNPDMIVKERYKLNDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDKLETENISVMYTVPTMLEALYKE
NRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEV
QKGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPA
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKSFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIEN
>Mature_552_residues
GDDESECCVFTRGLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIACFFLVFCKERTSHMGITKEYKK
HASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNKKESKNKTIAIVLENRIEFLQLFAGAAMTGWVCVPLDIKWKQDEL
KERIAISNPDMIVKERYKLNDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSWL
HSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDKLETENISVMYTVPTMLEALYKEN
RVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEVQ
KGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPAV
DEIVVIGVEDSYWGEKPVAIVKGSATRQQLKSFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIEN

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=356, Percent_Identity=27.8089887640449, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI187761345, Length=516, Percent_Identity=25, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI187761343, Length=516, Percent_Identity=25, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI42544132, Length=284, Percent_Identity=28.169014084507, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI157311624, Length=495, Percent_Identity=23.2323232323232, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI157311622, Length=495, Percent_Identity=23.2323232323232, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI58082049, Length=501, Percent_Identity=23.1536926147705, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI38505220, Length=357, Percent_Identity=25.2100840336134, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI122937307, Length=360, Percent_Identity=26.1111111111111, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI115511026, Length=495, Percent_Identity=23.030303030303, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI13375727, Length=426, Percent_Identity=20.8920187793427, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI28416953, Length=555, Percent_Identity=23.7837837837838, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788107, Length=526, Percent_Identity=24.5247148288973, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI145693145, Length=503, Percent_Identity=24.0556660039761, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1786810, Length=510, Percent_Identity=24.3137254901961, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI221142682, Length=202, Percent_Identity=32.6732673267327, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788595, Length=333, Percent_Identity=26.1261261261261, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1790505, Length=399, Percent_Identity=23.0576441102757, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=526, Percent_Identity=23.9543726235741, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=527, Percent_Identity=23.719165085389, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32563687, Length=486, Percent_Identity=25.7201646090535, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=300, Percent_Identity=31, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=365, Percent_Identity=28.2191780821918, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71994703, Length=349, Percent_Identity=26.9340974212034, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=349, Percent_Identity=26.9340974212034, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=361, Percent_Identity=27.1468144044321, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=349, Percent_Identity=26.9340974212034, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=364, Percent_Identity=28.021978021978, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=495, Percent_Identity=24.4444444444444, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=495, Percent_Identity=24.2424242424242, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=491, Percent_Identity=23.8289205702648, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25141359, Length=486, Percent_Identity=21.3991769547325, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=530, Percent_Identity=25.2830188679245, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6319264, Length=559, Percent_Identity=23.9713774597496, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24581924, Length=341, Percent_Identity=28.1524926686217, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21355181, Length=475, Percent_Identity=25.6842105263158, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648260, Length=497, Percent_Identity=23.5412474849095, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI18859661, Length=498, Percent_Identity=24.2971887550201, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356947, Length=229, Percent_Identity=26.6375545851528, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24653035, Length=200, Percent_Identity=28.5, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356441, Length=505, Percent_Identity=21.980198019802, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648253, Length=509, Percent_Identity=22.7897838899804, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648255, Length=509, Percent_Identity=22.5933202357564, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21358303, Length=136, Percent_Identity=33.0882352941176, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19922652, Length=353, Percent_Identity=23.2294617563739, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24656500, Length=344, Percent_Identity=25.2906976744186, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI161076582, Length=205, Percent_Identity=28.2926829268293, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20130357, Length=201, Percent_Identity=26.3681592039801, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI62472339, Length=401, Percent_Identity=25.9351620947631, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24667955, Length=401, Percent_Identity=25.9351620947631, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24667959, Length=401, Percent_Identity=25.9351620947631, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281365686, Length=226, Percent_Identity=25.6637168141593, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648257, Length=199, Percent_Identity=25.6281407035176, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21355225, Length=438, Percent_Identity=22.3744292237443, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 63931; Mature: 63800

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDDESECCVFTRGLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIAC
CCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCHHHHH
FFLVFCKERTSHMGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNKKES
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHCCCC
KNKTIAIVLENRIEFLQLFAGAAMTGWVCVPLDIKWKQDELKERIAISNPDMIVKERYKL
CCCEEEEEEHHHHHHHHHHHCCHHCCCEEEEEEECCCHHHHHHHHCCCCCCCEECCCCCC
NDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
CCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDKLE
HHHCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TENISVMYTVPTMLEALYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGA
CCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHEECCCC
SELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEVQKGEIGTVYVKSDQFFMGYI
CHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEEECHHCCHHHCCCCEEEEEECCCEEEHHH
IDGVLARELNADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPA
HHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCC
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKSFCLQRLSSFKIPKEWYFVDEIPYTNSG
CCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
KIARMEAKSIIEN
CEEEEEHHHHHCC
>Mature Secondary Structure 
GDDESECCVFTRGLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIAC
CCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCHHHHH
FFLVFCKERTSHMGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNKKES
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHCCCC
KNKTIAIVLENRIEFLQLFAGAAMTGWVCVPLDIKWKQDELKERIAISNPDMIVKERYKL
CCCEEEEEEHHHHHHHHHHHCCHHCCCEEEEEEECCCHHHHHHHHCCCCCCCEECCCCCC
NDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
CCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDKLE
HHHCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TENISVMYTVPTMLEALYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGA
CCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHEECCCC
SELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEVQKGEIGTVYVKSDQFFMGYI
CHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEEECHHCCHHHCCCCEEEEEECCCEEEHHH
IDGVLARELNADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPA
HHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCC
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKSFCLQRLSSFKIPKEWYFVDEIPYTNSG
CCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
KIARMEAKSIIEN
CEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]