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The map label for this gene is qor [C]

Identifier: 118478724

GI number: 118478724

Start: 3295033

End: 3296067

Strand: Direct

Name: qor [C]

Synonym: BALH_3111

Alternate gene names: 118478724

Gene position: 3295033-3296067 (Clockwise)

Preceding gene: 118478719

Following gene: 118478727

Centisome position: 62.68

GC content: 35.27

Gene sequence:

>1035_bases
ATGGAGGGAATACAAATGAAAGCAATTGGTTTACATGAATACTTACCTATTGAAGAAGAAAATAGTTTAATTGATATCGA
AGTTGAAAGACCTGTTGCTACAGGAAGAGATATACTCGTTAAAATTAATGCCATTTCCGTTAATCCAGTTGATACAAAAG
TTCGCTCTCCAAAAGATAAAAAAGAAGATGTAGCGAAAATACTTGGCTGGGATGCTAGTGGTGTTGTCGTGCAAACCGGC
GAAAGTTGTACGTTATTTAAAGAAGGCGACGAAGTGTTTTACGCTGGAAGCATTACGAGACAAGGTACATATAGTGAATA
TCATTTAGTTGATGAAAGAATTGTTGGTAAAAAACCAAAAACGTTAAGTGATGCTGAATCTGCAGCACTTCCTTTAACAG
CTATTACGGCTTGGGAAGGTTTATTCGAACGTTTAGGCATTGATTATAATAAAAAAGATACAAATACATTTAAAAACATT
TTAATTATTGGGGGAGCTGGTGGTGTCGGTTCAATCGCAATTCAATTAGCAAAATGGGCTGGACTAAATGTAATCACAAC
TGCATCCCGCACTGAGACGATACACTGGGTTGAAAAATTCGGTGCCGATTATATTATTAACCATCACCAACCTTTACAAG
ATCAAATATTAGAATTCGGGATAAAAGATGTAGATTATATTTTCTGCTTAAACAATACAGATCAACATTGGCAAGCAATA
TGTGAACTTATTAAACCACAAGGAAAAATTTGCTCTATCGTAGAAAATGAGCGCCCTCTTGAAATGGGTATTTTAAAAAG
TAAAAGTGCTACACTCGTTTGGGAATTTATGTTTACAAAAGCGATGTATGAAACTGGCGATATGATTACGCAGCACGAAC
TATTAAATAAAGTAAGCGAACTATTAGATGAGGGCATTTTAAAGACTACATTAAATGAAACACTAACACCAATTAACGCT
GAAAACGTAAAGAAAGCACATGCCCTGCTTGAAAGCGGCAGTACAATTGGGAAGATCGTTTTAGAGAAATTTTAA

Upstream 100 bases:

>100_bases
TAATGAGGTTGAAACGGATATTTTACAAAACGGAAAAGACCCATCTTTTTACATAATGACACTTTACCTACTATAATAAA
AAAGAAAAACTCTTACTTTA

Downstream 100 bases:

>100_bases
TAATAAAAAGTCCGATTTCTAAATGAGAAATCGGACTTTTTACTATCTTTATTTACTTGAAATCTCCTGATTATTTGGAA
CATCTAACGGACGTCGTTTC

Product: alginate lyase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MEGIQMKAIGLHEYLPIEEENSLIDIEVERPVATGRDILVKINAISVNPVDTKVRSPKDKKEDVAKILGWDASGVVVQTG
ESCTLFKEGDEVFYAGSITRQGTYSEYHLVDERIVGKKPKTLSDAESAALPLTAITAWEGLFERLGIDYNKKDTNTFKNI
LIIGGAGGVGSIAIQLAKWAGLNVITTASRTETIHWVEKFGADYIINHHQPLQDQILEFGIKDVDYIFCLNNTDQHWQAI
CELIKPQGKICSIVENERPLEMGILKSKSATLVWEFMFTKAMYETGDMITQHELLNKVSELLDEGILKTTLNETLTPINA
ENVKKAHALLESGSTIGKIVLEKF

Sequences:

>Translated_344_residues
MEGIQMKAIGLHEYLPIEEENSLIDIEVERPVATGRDILVKINAISVNPVDTKVRSPKDKKEDVAKILGWDASGVVVQTG
ESCTLFKEGDEVFYAGSITRQGTYSEYHLVDERIVGKKPKTLSDAESAALPLTAITAWEGLFERLGIDYNKKDTNTFKNI
LIIGGAGGVGSIAIQLAKWAGLNVITTASRTETIHWVEKFGADYIINHHQPLQDQILEFGIKDVDYIFCLNNTDQHWQAI
CELIKPQGKICSIVENERPLEMGILKSKSATLVWEFMFTKAMYETGDMITQHELLNKVSELLDEGILKTTLNETLTPINA
ENVKKAHALLESGSTIGKIVLEKF
>Mature_344_residues
MEGIQMKAIGLHEYLPIEEENSLIDIEVERPVATGRDILVKINAISVNPVDTKVRSPKDKKEDVAKILGWDASGVVVQTG
ESCTLFKEGDEVFYAGSITRQGTYSEYHLVDERIVGKKPKTLSDAESAALPLTAITAWEGLFERLGIDYNKKDTNTFKNI
LIIGGAGGVGSIAIQLAKWAGLNVITTASRTETIHWVEKFGADYIINHHQPLQDQILEFGIKDVDYIFCLNNTDQHWQAI
CELIKPQGKICSIVENERPLEMGILKSKSATLVWEFMFTKAMYETGDMITQHELLNKVSELLDEGILKTTLNETLTPINA
ENVKKAHALLESGSTIGKIVLEKF

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=334, Percent_Identity=30.2395209580838, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI194239676, Length=206, Percent_Identity=31.0679611650485, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI194239674, Length=206, Percent_Identity=31.0679611650485, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI13236495, Length=206, Percent_Identity=31.0679611650485, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI22538446, Length=353, Percent_Identity=25.7790368271955, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI22538444, Length=353, Percent_Identity=25.7790368271955, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1790485, Length=314, Percent_Identity=28.0254777070064, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17507255, Length=184, Percent_Identity=32.0652173913043, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71987554, Length=192, Percent_Identity=31.7708333333333, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6319520, Length=325, Percent_Identity=29.2307692307692, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR014182
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 38232; Mature: 38232

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGIQMKAIGLHEYLPIEEENSLIDIEVERPVATGRDILVKINAISVNPVDTKVRSPKDK
CCCCEEEEECHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEEEECCCCCCCCCCCCH
KEDVAKILGWDASGVVVQTGESCTLFKEGDEVFYAGSITRQGTYSEYHLVDERIVGKKPK
HHHHHHHHCCCCCCEEEECCCCEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCC
TLSDAESAALPLTAITAWEGLFERLGIDYNKKDTNTFKNILIIGGAGGVGSIAIQLAKWA
CCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHC
GLNVITTASRTETIHWVEKFGADYIINHHQPLQDQILEFGIKDVDYIFCLNNTDQHWQAI
CCEEEEECCCCHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCCCEEEEEECCCCHHHHHH
CELIKPQGKICSIVENERPLEMGILKSKSATLVWEFMFTKAMYETGDMITQHELLNKVSE
HHHHCCCCCEEEECCCCCCCEEEEECCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLDEGILKTTLNETLTPINAENVKKAHALLESGSTIGKIVLEKF
HHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHEECC
>Mature Secondary Structure
MEGIQMKAIGLHEYLPIEEENSLIDIEVERPVATGRDILVKINAISVNPVDTKVRSPKDK
CCCCEEEEECHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEEEECCCCCCCCCCCCH
KEDVAKILGWDASGVVVQTGESCTLFKEGDEVFYAGSITRQGTYSEYHLVDERIVGKKPK
HHHHHHHHCCCCCCEEEECCCCEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCC
TLSDAESAALPLTAITAWEGLFERLGIDYNKKDTNTFKNILIIGGAGGVGSIAIQLAKWA
CCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHC
GLNVITTASRTETIHWVEKFGADYIINHHQPLQDQILEFGIKDVDYIFCLNNTDQHWQAI
CCEEEEECCCCHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCCCEEEEEECCCCHHHHHH
CELIKPQGKICSIVENERPLEMGILKSKSATLVWEFMFTKAMYETGDMITQHELLNKVSE
HHHHCCCCCEEEECCCCCCCEEEEECCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLDEGILKTTLNETLTPINAENVKKAHALLESGSTIGKIVLEKF
HHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]