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The map label for this gene is emrB [H]

Identifier: 118478224

GI number: 118478224

Start: 2742390

End: 2743925

Strand: Reverse

Name: emrB [H]

Synonym: BALH_2583

Alternate gene names: 118478224

Gene position: 2743925-2742390 (Counterclockwise)

Preceding gene: 118478225

Following gene: 118478221

Centisome position: 52.19

GC content: 36.78

Gene sequence:

>1536_bases
ATGGTTGAGAAGAATAATAAGCTCGGATTTGTTGTGGCGGGCTTATTGCTAGGTATTTTAATGGCATCAATGGATAATAC
CATTGTCGTAACAGCAATGGGAACGATTGTTGGTGACTTAGGAGGCCTTGAAAACTTTGTATGGGTCGTTTCGGCCTATA
TGGTCGCAGAAATGGCAGGGATGCCGATTTTCGGTAAATTATCAGATATGTATGGTAGAAAGAGATTCTTTATTTTCGGT
TTAATCGTCTTTATGATTGGTTCGGCACTTTGTGGTACTGCTGAAAATATTACACAGCTAGGTATTTATCGTGCCATTCA
AGGTATTGGCGGCGGGGCATTAGTGCCGATCGCGTTTACTATCGTTTTTGATATTTTCCCTCCTGAAAAACGCGGGAAAA
TGGGTGGTTTATTCGGAGCAGTATTTGGTTTATCAAGTATTTTCGGGCCATTACTTGGCGCATATATTACAGATTACATT
AGCTGGCACTGGGTATTTTATATTAACTTACCGCTTGGCGTTTTAGCACTTATTTTTATTACGTTCTTTTATAAAGAATC
AAGAGTTCATAGAAAGCAAAAAATTGATTGGTTTGGTGCAATTACTTTAGTTGGTGCAGTAATTTGTTTAATGTTTGCTT
TAGAACTTGGCGGACAAAAGTATGACTGGGATTCTAGCTTTATTTTAAGTTTATTTGGTGGATTCGCTATTTTAATTATT
GCATTTATATTTATTGAACGTAAAGTAGAAGAACCGATCATTTCATTTGAGATGTTTAAGCAGCGCTTATTCGGAATGAG
TACCATTATTGCTTTATGTTACGGTGCTGCGTTTATGTCAGCAACTGTATACATTCCGTTATTCATTCAAGGTGTATACG
GCGGTAGTGCAACAAATTCAGGGTTATTACTATTGCCAATGATGTTAGGATCAGTTGTAACAGCGCAATTAGGTGGATTT
TTAACGACGAAGCTTAGCTACAGAAACATTATGATTATTTCTGCGGTTATTATGCTAATTGGACTATTTTTATTAAGCAC
ATTAACGCCAGAAACAAGTCGCGCATTATTAACAGTGTATATGATTATTATCGGATTTGGAGTTGGATTCTCATTTTCTG
TACTAAGTATGGCAGCTATTCACAACTTTGGTATGGAGCAACGTGGATCTGCGACTTCAACGAGTAATTTCATTCGTTCA
TTAGGAATGACGCTTGGTATTACAATCTTCGGAATGATTCAAAGAACAGGTTTCCAAGATCAATTAGAAGAAGCATTTAA
AGGGATGAGCGGGGGAATGAATACAAACGCTTTAGGAGATTCAAGAGCTATTTTATCAGAATCGGCAAGATCTCAAATCC
CGCCGCAAATATTAGATAAAATTATTAACGCTCTTTCTAGCTCAATTGTCCAAACATTTATGTGGGCATTGGTACCAGCT
GGTTTAGCATTCTTATTTATTTTCTTTATGGGAAATGAGCGCATGGTAATGAAGAAACAACAAAAGAATAAAAAGAACGA
AACATCAAAAGCGTAA

Upstream 100 bases:

>100_bases
AATTAGTGTTGTAGAATATCATACTAGTTTTAAGCAAAAAGGTAATGCAGTCGTAAAAGAACATAAACAAATATAAGTAG
TATGAGGGAGAGCAAGAGAT

Downstream 100 bases:

>100_bases
TGAAAAAGCTCCTTTCCAATTGGAAAGGAGCTTTTTCGTATGTACGGTTTTAGATTAGCCTTCGATGATTTCGTATTCTT
CGTCTGCTAATAATAAAATT

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG
LIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYI
SWHWVFYINLPLGVLALIFITFFYKESRVHRKQKIDWFGAITLVGAVICLMFALELGGQKYDWDSSFILSLFGGFAILII
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGSATNSGLLLLPMMLGSVVTAQLGGF
LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVYMIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRS
LGMTLGITIFGMIQRTGFQDQLEEAFKGMSGGMNTNALGDSRAILSESARSQIPPQILDKIINALSSSIVQTFMWALVPA
GLAFLFIFFMGNERMVMKKQQKNKKNETSKA

Sequences:

>Translated_511_residues
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG
LIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYI
SWHWVFYINLPLGVLALIFITFFYKESRVHRKQKIDWFGAITLVGAVICLMFALELGGQKYDWDSSFILSLFGGFAILII
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGSATNSGLLLLPMMLGSVVTAQLGGF
LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVYMIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRS
LGMTLGITIFGMIQRTGFQDQLEEAFKGMSGGMNTNALGDSRAILSESARSQIPPQILDKIINALSSSIVQTFMWALVPA
GLAFLFIFFMGNERMVMKKQQKNKKNETSKA
>Mature_511_residues
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAGMPIFGKLSDMYGRKRFFIFG
LIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFTIVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYI
SWHWVFYINLPLGVLALIFITFFYKESRVHRKQKIDWFGAITLVGAVICLMFALELGGQKYDWDSSFILSLFGGFAILII
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGSATNSGLLLLPMMLGSVVTAQLGGF
LTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVYMIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRS
LGMTLGITIFGMIQRTGFQDQLEEAFKGMSGGMNTNALGDSRAILSESARSQIPPQILDKIINALSSSIVQTFMWALVPA
GLAFLFIFFMGNERMVMKKQQKNKKNETSKA

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=403, Percent_Identity=30.5210918114144, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87081983, Length=417, Percent_Identity=28.537170263789, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788710, Length=441, Percent_Identity=28.1179138321995, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1790195, Length=421, Percent_Identity=24.7030878859857, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1788392, Length=405, Percent_Identity=25.679012345679, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790146, Length=162, Percent_Identity=29.6296296296296, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI48994889, Length=134, Percent_Identity=31.3432835820896, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=554, Percent_Identity=23.826714801444, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319770, Length=399, Percent_Identity=27.8195488721804, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319783, Length=457, Percent_Identity=24.0700218818381, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6325455, Length=529, Percent_Identity=23.2514177693762, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6323735, Length=324, Percent_Identity=30.8641975308642, Blast_Score=112, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6321663, Length=540, Percent_Identity=22.7777777777778, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324954, Length=398, Percent_Identity=24.8743718592965, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323520, Length=410, Percent_Identity=24.6341463414634, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55787; Mature: 55787

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAG
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHC
MPIFGKLSDMYGRKRFFIFGLIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFT
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
IVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYISWHWVFYINLPLGVLALIFI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHH
TFFYKESRVHRKQKIDWFGAITLVGAVICLMFALELGGQKYDWDSSFILSLFGGFAILII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGSATNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
GLLLLPMMLGSVVTAQLGGFLTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVY
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRSLGMTLGITIFGMIQRTGFQD
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QLEEAFKGMSGGMNTNALGDSRAILSESARSQIPPQILDKIINALSSSIVQTFMWALVPA
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAFLFIFFMGNERMVMKKQQKNKKNETSKA
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVEKNNKLGFVVAGLLLGILMASMDNTIVVTAMGTIVGDLGGLENFVWVVSAYMVAEMAG
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHC
MPIFGKLSDMYGRKRFFIFGLIVFMIGSALCGTAENITQLGIYRAIQGIGGGALVPIAFT
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
IVFDIFPPEKRGKMGGLFGAVFGLSSIFGPLLGAYITDYISWHWVFYINLPLGVLALIFI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHH
TFFYKESRVHRKQKIDWFGAITLVGAVICLMFALELGGQKYDWDSSFILSLFGGFAILII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AFIFIERKVEEPIISFEMFKQRLFGMSTIIALCYGAAFMSATVYIPLFIQGVYGGSATNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
GLLLLPMMLGSVVTAQLGGFLTTKLSYRNIMIISAVIMLIGLFLLSTLTPETSRALLTVY
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MIIIGFGVGFSFSVLSMAAIHNFGMEQRGSATSTSNFIRSLGMTLGITIFGMIQRTGFQD
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QLEEAFKGMSGGMNTNALGDSRAILSESARSQIPPQILDKIINALSSSIVQTFMWALVPA
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAFLFIFFMGNERMVMKKQQKNKKNETSKA
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]