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The map label for this gene is vanS [H]

Identifier: 118477337

GI number: 118477337

Start: 1801436

End: 1802563

Strand: Direct

Name: vanS [H]

Synonym: BALH_1654

Alternate gene names: 118477337

Gene position: 1801436-1802563 (Clockwise)

Preceding gene: 118477336

Following gene: 118477338

Centisome position: 34.27

GC content: 31.56

Gene sequence:

>1128_bases
TTGAAGAGAATCCAAAAGAGCCGAAGTTTATACAGAATGTTTGGGGAGTCGGATATAAAATTGAAGATTAAATCAGTACA
AGGCATTAGAGCTAAGTTTTTTATCGCATTCATATGTAGCATCTTATTAGCCACTGTAAGTATAATAGCGTTTCAAATTG
TAATTGGAAATATATATAGTCATGTTACTGCGTTAGAAGAAAAATATTCACTTTTATATGTAATAGCTTTTTTAACTTTT
ACTACAATATACTTTGCATTCATGACAAAAACAATGATGAAAAGATTATCTGAAATTAACAAGAGTGTGAAGGAAATAAG
TAGTGGTAATTTGGAAATACATATACCTATTTCAAAAAATGACGAGATTGGTGAATTAGCGAAGAATGTAAATAGAATGG
CCAAAAGTTTAAAAGAGTCTATCGAGAATGAAAAAAAATCGCAGGAAATGAAAAATGAAATGATTAGTAATATTTCGCAT
GACTTACGAACACCTGTAACATCTCTAATCGGTTACGCGGACTTGTTAGGAAGTCAGTTTCATACAAATAGAGAAGAATG
TGAACAGTATGTAAGCATATTAAAGCGAAAAAGTTATGAATTAAAAAATCAAGTAGATGATTTATTAGAATACTGTCAAA
TAAATTATAGAGAGATTGAATTACATAAAAGTGTAGTAAATATGAAAGCATTAATGGAACAAATTATGATTGATTTTGTA
CCACAACTAGATGATGCCGATATGAGCTTTTGTATAAAAGGTGATAAGGAGTTACATGTGGAAATGGATGTAGCGCTTAT
GGTGAGGGTATTCGAAAATGTAATTAGTAATAGTATTATGTACGGGAAAGACGGAAAACAGATTTTGATTCAAATTTCTA
AAAGAGACCTGAATGTTGAAATAGAAATTAAAAATTTTGGACAATGTATTCCGAATGAGAACCTTCCTTACGTTTTTGAG
AAGTTTTATCGTGGTGAAAAATCACGCAGCTCTCATACAGGTGGAAAAGGAATGGGGCTTGCAATTGCGAGAAGTATAGC
AGAGCTTCATAAAGGGGATATCACTGTGCGCAGTAACGAGAAAGAAACTGTTTTCACTATCGCTTTACCGCGTTATAAAG
AATTGTAA

Upstream 100 bases:

>100_bases
GTTTTTACTATTGAAGAAATATATGAAAGCGTTTGGAAAGAAAGAGCCTATGAAAGTAATAATACAGTAATGGTTCATAT
CGCAAGATTAAGAAATAAAA

Downstream 100 bases:

>100_bases
GAAAGTCGTAAGTATTTCTTTTATATGTCGTAATTTTCACTTCGTATTATGTAGAACAAAGATATGAGAGTGGGGATACA
ACATGTGGAAGAAATATGTA

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKRIQKSRSLYRMFGESDIKLKIKSVQGIRAKFFIAFICSILLATVSIIAFQIVIGNIYSHVTALEEKYSLLYVIAFLTF
TTIYFAFMTKTMMKRLSEINKSVKEISSGNLEIHIPISKNDEIGELAKNVNRMAKSLKESIENEKKSQEMKNEMISNISH
DLRTPVTSLIGYADLLGSQFHTNREECEQYVSILKRKSYELKNQVDDLLEYCQINYREIELHKSVVNMKALMEQIMIDFV
PQLDDADMSFCIKGDKELHVEMDVALMVRVFENVISNSIMYGKDGKQILIQISKRDLNVEIEIKNFGQCIPNENLPYVFE
KFYRGEKSRSSHTGGKGMGLAIARSIAELHKGDITVRSNEKETVFTIALPRYKEL

Sequences:

>Translated_375_residues
MKRIQKSRSLYRMFGESDIKLKIKSVQGIRAKFFIAFICSILLATVSIIAFQIVIGNIYSHVTALEEKYSLLYVIAFLTF
TTIYFAFMTKTMMKRLSEINKSVKEISSGNLEIHIPISKNDEIGELAKNVNRMAKSLKESIENEKKSQEMKNEMISNISH
DLRTPVTSLIGYADLLGSQFHTNREECEQYVSILKRKSYELKNQVDDLLEYCQINYREIELHKSVVNMKALMEQIMIDFV
PQLDDADMSFCIKGDKELHVEMDVALMVRVFENVISNSIMYGKDGKQILIQISKRDLNVEIEIKNFGQCIPNENLPYVFE
KFYRGEKSRSSHTGGKGMGLAIARSIAELHKGDITVRSNEKETVFTIALPRYKEL
>Mature_375_residues
MKRIQKSRSLYRMFGESDIKLKIKSVQGIRAKFFIAFICSILLATVSIIAFQIVIGNIYSHVTALEEKYSLLYVIAFLTF
TTIYFAFMTKTMMKRLSEINKSVKEISSGNLEIHIPISKNDEIGELAKNVNRMAKSLKESIENEKKSQEMKNEMISNISH
DLRTPVTSLIGYADLLGSQFHTNREECEQYVSILKRKSYELKNQVDDLLEYCQINYREIELHKSVVNMKALMEQIMIDFV
PQLDDADMSFCIKGDKELHVEMDVALMVRVFENVISNSIMYGKDGKQILIQISKRDLNVEIEIKNFGQCIPNENLPYVFE
KFYRGEKSRSSHTGGKGMGLAIARSIAELHKGDITVRSNEKETVFTIALPRYKEL

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=273, Percent_Identity=28.2051282051282, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788393, Length=287, Percent_Identity=25.0871080139373, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786600, Length=231, Percent_Identity=25.1082251082251, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786912, Length=225, Percent_Identity=25.7777777777778, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787894, Length=304, Percent_Identity=25.3289473684211, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789149, Length=255, Percent_Identity=22.7450980392157, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=23.7704918032787, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082128, Length=235, Percent_Identity=25.9574468085106, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788279, Length=263, Percent_Identity=26.9961977186312, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI48994928, Length=234, Percent_Identity=26.0683760683761, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790346, Length=285, Percent_Identity=21.0526315789474, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790551, Length=325, Percent_Identity=22.1538461538462, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790861, Length=235, Percent_Identity=21.2765957446809, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788549, Length=221, Percent_Identity=24.8868778280543, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 43111; Mature: 43111

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIQKSRSLYRMFGESDIKLKIKSVQGIRAKFFIAFICSILLATVSIIAFQIVIGNIYS
CCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVTALEEKYSLLYVIAFLTFTTIYFAFMTKTMMKRLSEINKSVKEISSGNLEIHIPISKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
DEIGELAKNVNRMAKSLKESIENEKKSQEMKNEMISNISHDLRTPVTSLIGYADLLGSQF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HTNREECEQYVSILKRKSYELKNQVDDLLEYCQINYREIELHKSVVNMKALMEQIMIDFV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PQLDDADMSFCIKGDKELHVEMDVALMVRVFENVISNSIMYGKDGKQILIQISKRDLNVE
CCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEECCCCEEE
IEIKNFGQCIPNENLPYVFEKFYRGEKSRSSHTGGKGMGLAIARSIAELHKGDITVRSNE
EEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCC
KETVFTIALPRYKEL
CCEEEEEECCCCCCC
>Mature Secondary Structure
MKRIQKSRSLYRMFGESDIKLKIKSVQGIRAKFFIAFICSILLATVSIIAFQIVIGNIYS
CCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVTALEEKYSLLYVIAFLTFTTIYFAFMTKTMMKRLSEINKSVKEISSGNLEIHIPISKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
DEIGELAKNVNRMAKSLKESIENEKKSQEMKNEMISNISHDLRTPVTSLIGYADLLGSQF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HTNREECEQYVSILKRKSYELKNQVDDLLEYCQINYREIELHKSVVNMKALMEQIMIDFV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PQLDDADMSFCIKGDKELHVEMDVALMVRVFENVISNSIMYGKDGKQILIQISKRDLNVE
CCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEECCCCEEE
IEIKNFGQCIPNENLPYVFEKFYRGEKSRSSHTGGKGMGLAIARSIAELHKGDITVRSNE
EEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCC
KETVFTIALPRYKEL
CCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]