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The map label for this gene is ypcP [H]

Identifier: 118477127

GI number: 118477127

Start: 1571079

End: 1571984

Strand: Direct

Name: ypcP [H]

Synonym: BALH_1429

Alternate gene names: 118477127

Gene position: 1571079-1571984 (Clockwise)

Preceding gene: 118477126

Following gene: 118477129

Centisome position: 29.88

GC content: 36.53

Gene sequence:

>906_bases
ATGACGTGGGGTTATTATTTTTTTGGTAGGAGAGGAATTATGAAAAAAGTATTATTAGTTGATGGTATGGCACTATTATT
TCGTGCTTTTTATGCAACAAGTGTCTACGGACAGTTTATGAAACGACAAGATGGTACCCCTACAAACGGGATTCATGGTT
ATATGAAACATTTATTAACAGCAATGCAAGCAATTGAACCGACTCATATCGTAACATGCTGGGATATGGGGAGTACGACA
TTTAGAACAGAATCGTTCTCAAATTATAAAGCGAATCGTGCAGCGCCGCCTGAAGAATTAATTCCGCAATTTGATTTAGT
ACAAGAAATGACTGCGAAATTATCCGTGCCAGTCATCGGTATGAAAGGGTATGAAGCGGATGATTGTATCGGTACGCTTG
CGAAACAATATTGTAATGAAGCGGAAGTTTATATTTTAACAGGTGATACGGATTTACTTCAGCTTGTTGATAAAAATGTT
ACAGTTATGCTTCTGCGCAAAGGAATGGGAAATTATGAGTATTACACACCAGAGAAAATTATGGAAGAAAAAGGTGTAGA
ACCGTGGCAAATCGTGCATGCGAAAGCTTTCATGGGAGATACGAGCGATAATTATCCAGGTGTAAAAGGTATCGGTGAAA
AAACAGCATATAAGCTTATTCAAGAACATGGTACAGTAGCAACTGTACTAGAAAATGTGGCATCATTAACGAAAGCGCAA
CGTACGAAGATTGAAAGTGATTTAGAGAATTTAAATATTTCATTACAATTAGCGCAAATTCATTGTGAAGTTCCAATTTC
ATGTTCACTAGAAGAAGGATTACACACAATAGATGAAGAAAAACTACGATTCGTTTGTGAAGAAATGAATTGGGGAAGAC
CTGAAATGTTAATCAATATGCTGTAA

Upstream 100 bases:

>100_bases
ACGTCTTAATGTGTAAATGATGCGAAATATACAAAAACAATGTATAATAGACAAAGTTGAAAAAACGTAGAATGGTAATC
TATTTATATAACCTTACGTA

Downstream 100 bases:

>100_bases
ATACTCGGAAGACCGATTAAAAGTAAAATGAATCGGTCTTTCTTTTTTTGTTCACAAAGTTGTCAGAAAATAAACGGAAT
TTTCATGTGAAGTTCAAATG

Product: 5'-3' exonuclease

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTWGYYFFGRRGIMKKVLLVDGMALLFRAFYATSVYGQFMKRQDGTPTNGIHGYMKHLLTAMQAIEPTHIVTCWDMGSTT
FRTESFSNYKANRAAPPEELIPQFDLVQEMTAKLSVPVIGMKGYEADDCIGTLAKQYCNEAEVYILTGDTDLLQLVDKNV
TVMLLRKGMGNYEYYTPEKIMEEKGVEPWQIVHAKAFMGDTSDNYPGVKGIGEKTAYKLIQEHGTVATVLENVASLTKAQ
RTKIESDLENLNISLQLAQIHCEVPISCSLEEGLHTIDEEKLRFVCEEMNWGRPEMLINML

Sequences:

>Translated_301_residues
MTWGYYFFGRRGIMKKVLLVDGMALLFRAFYATSVYGQFMKRQDGTPTNGIHGYMKHLLTAMQAIEPTHIVTCWDMGSTT
FRTESFSNYKANRAAPPEELIPQFDLVQEMTAKLSVPVIGMKGYEADDCIGTLAKQYCNEAEVYILTGDTDLLQLVDKNV
TVMLLRKGMGNYEYYTPEKIMEEKGVEPWQIVHAKAFMGDTSDNYPGVKGIGEKTAYKLIQEHGTVATVLENVASLTKAQ
RTKIESDLENLNISLQLAQIHCEVPISCSLEEGLHTIDEEKLRFVCEEMNWGRPEMLINML
>Mature_300_residues
TWGYYFFGRRGIMKKVLLVDGMALLFRAFYATSVYGQFMKRQDGTPTNGIHGYMKHLLTAMQAIEPTHIVTCWDMGSTTF
RTESFSNYKANRAAPPEELIPQFDLVQEMTAKLSVPVIGMKGYEADDCIGTLAKQYCNEAEVYILTGDTDLLQLVDKNVT
VMLLRKGMGNYEYYTPEKIMEEKGVEPWQIVHAKAFMGDTSDNYPGVKGIGEKTAYKLIQEHGTVATVLENVASLTKAQR
TKIESDLENLNISLQLAQIHCEVPISCSLEEGLHTIDEEKLRFVCEEMNWGRPEMLINML

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=264, Percent_Identity=35.2272727272727, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI226510970, Length=210, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR008918 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 34058; Mature: 33927

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWGYYFFGRRGIMKKVLLVDGMALLFRAFYATSVYGQFMKRQDGTPTNGIHGYMKHLLT
CCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AMQAIEPTHIVTCWDMGSTTFRTESFSNYKANRAAPPEELIPQFDLVQEMTAKLSVPVIG
HHHHCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCEEE
MKGYEADDCIGTLAKQYCNEAEVYILTGDTDLLQLVDKNVTVMLLRKGMGNYEYYTPEKI
CCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
MEEKGVEPWQIVHAKAFMGDTSDNYPGVKGIGEKTAYKLIQEHGTVATVLENVASLTKAQ
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RTKIESDLENLNISLQLAQIHCEVPISCSLEEGLHTIDEEKLRFVCEEMNWGRPEMLINM
HHHHHHHHHHCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
L
C
>Mature Secondary Structure 
TWGYYFFGRRGIMKKVLLVDGMALLFRAFYATSVYGQFMKRQDGTPTNGIHGYMKHLLT
CCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AMQAIEPTHIVTCWDMGSTTFRTESFSNYKANRAAPPEELIPQFDLVQEMTAKLSVPVIG
HHHHCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCEEE
MKGYEADDCIGTLAKQYCNEAEVYILTGDTDLLQLVDKNVTVMLLRKGMGNYEYYTPEKI
CCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
MEEKGVEPWQIVHAKAFMGDTSDNYPGVKGIGEKTAYKLIQEHGTVATVLENVASLTKAQ
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RTKIESDLENLNISLQLAQIHCEVPISCSLEEGLHTIDEEKLRFVCEEMNWGRPEMLINM
HHHHHHHHHHCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]