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The map label for this gene is cca [H]

Identifier: 118477089

GI number: 118477089

Start: 1528063

End: 1529265

Strand: Direct

Name: cca [H]

Synonym: BALH_1390

Alternate gene names: 118477089

Gene position: 1528063-1529265 (Clockwise)

Preceding gene: 118477088

Following gene: 118477090

Centisome position: 29.07

GC content: 37.32

Gene sequence:

>1203_bases
ATGACAAAAATGGAAATATTTAAAAAAGCTAGTTCTATTATTGAGACTTTAAAGCAACAAGGACATGAGGCTTACTTTGT
CGGTGGAAGCGTAAGAGATCTTATTATCGATAGGCCGATTGGAGATATTGATATTGCGACATCTGCTTTACCAGAGGAAG
TGATGGGGATATTTCCAAGACATGTTCCTGTTGGTCTTGAACATGGAACTGTAATTGTAGTAGAAAATGGTGAGCCTTAT
GAGGTAACCACTTTTCGAACAGAGAGTGAATATGAAGATTTTCGAAGACCTAGTAGTGTTCAATTTGTTCGTTCGTTAGA
AGAGGATTTAAAACGTCGTGATTTCACAATGAATGCAATTGCCATGACGGAAGAAGGCGAAATGGTTGATTTATTTGCAG
GACAGGAAGCGATTCAAAAGAGAGAAATTGTGACAGTGGGAAATGCTGCGGATCGTTTTCAAGAAGATGCCCTGCGAATG
ATGCGTGGTATTCGGTTCGTAAGTACGTTAGGTTTTTCTTTAGAGACGAAAACGAAACAAGCAATTGAAACATATGGACA
TTTGCTGGAACATATAGCAATTGAGAGAATTACAGTGGAGTTTGAAAAACTGTTAACTGGCACATATTGCGTGAAGGGTC
TCAAAGAATTAGTGGAAACGAAACTATTTTCTCATCTGCCATATCTACAAATGTCAGAAGAGAGATTATTAAAAGCTACG
CAGTATAACTGGGATTCTTTTGAAACAGACATTGAGGCATGGGCGTTTTTCTTATATTGTATTGGAGAAGAACATCCATC
TGTCTTTTTACGTCAATGGAAGTTTTCAAATAAAAAAATAAAAGATATCGTCGCAGTTTTATTAACAATCCGTAAGAGAA
AGGAGAAGGATTGGGATACAGTCCTTCTTTATAAAACGGGAATTCATATAGCTGAAATGTCAGAAAGAGTATATGAAGCG
ATGATTGAAAGCTATGACAATACATCTGTGAAACGAGTGCAAACGTTGTTTGAAGCATTGCCAATCAAGAGTCGACAAGA
AATGAATGTGACTGGTAATGATTTATTAAACTGGGCAAATAAAAAACCAGGTCCGTGGGTTGCTGAAATGATTCAAAACA
TCGAGGAAGCAATCGTACAAGGAAACGTAGTGAATGAGAAAGAGTGTATAAGGGAGTGGCTGCAAGAATGCAATCTACTA
TAA

Upstream 100 bases:

>100_bases
CGTAATATGGGAGAGCGAGCAAGAGCAAGTGTCTACGAGCAGTTCCGCTCTGAAAAAATCGTATCACAATATGAAACGAT
TTACTATGATATACTAAGGG

Downstream 100 bases:

>100_bases
GAAAGCAGTTATTGCAAGTTTTTTCTGAGGCGGATGGAGAGTTTGTATCTGGCCAAACGATTAGTGATAAACTTGGTTGC
TCGAGAACCGCTGTGTGGAA

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTKMEIFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMGIFPRHVPVGLEHGTVIVVENGEPY
EVTTFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGEMVDLFAGQEAIQKREIVTVGNAADRFQEDALRM
MRGIRFVSTLGFSLETKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKAT
QYNWDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLLYKTGIHIAEMSERVYEA
MIESYDNTSVKRVQTLFEALPIKSRQEMNVTGNDLLNWANKKPGPWVAEMIQNIEEAIVQGNVVNEKECIREWLQECNLL

Sequences:

>Translated_400_residues
MTKMEIFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMGIFPRHVPVGLEHGTVIVVENGEPY
EVTTFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGEMVDLFAGQEAIQKREIVTVGNAADRFQEDALRM
MRGIRFVSTLGFSLETKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKAT
QYNWDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLLYKTGIHIAEMSERVYEA
MIESYDNTSVKRVQTLFEALPIKSRQEMNVTGNDLLNWANKKPGPWVAEMIQNIEEAIVQGNVVNEKECIREWLQECNLL
>Mature_399_residues
TKMEIFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMGIFPRHVPVGLEHGTVIVVENGEPYE
VTTFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGEMVDLFAGQEAIQKREIVTVGNAADRFQEDALRMM
RGIRFVSTLGFSLETKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQ
YNWDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLLYKTGIHIAEMSERVYEAM
IESYDNTSVKRVQTLFEALPIKSRQEMNVTGNDLLNWANKKPGPWVAEMIQNIEEAIVQGNVVNEKECIREWLQECNLL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=206, Percent_Identity=32.5242718446602, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI87081691, Length=237, Percent_Identity=32.0675105485232, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789436, Length=190, Percent_Identity=32.6315789473684, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=268, Percent_Identity=29.4776119402985, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI281360112, Length=269, Percent_Identity=29.7397769516729, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357337, Length=269, Percent_Identity=29.7397769516729, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46241; Mature: 46109

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKMEIFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMGIFPR
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCHHHHHHHCHHHHHHHCCC
HVPVGLEHGTVIVVENGEPYEVTTFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAI
CCCCCCCCCEEEEEECCCCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEE
AMTEEGEMVDLFAGQEAIQKREIVTVGNAADRFQEDALRMMRGIRFVSTLGFSLETKTKQ
EEECCCCEEEECCCHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AIETYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHH
QYNWDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDT
HCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCE
VLLYKTGIHIAEMSERVYEAMIESYDNTSVKRVQTLFEALPIKSRQEMNVTGNDLLNWAN
EEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCC
KKPGPWVAEMIQNIEEAIVQGNVVNEKECIREWLQECNLL
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKMEIFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMGIFPR
CHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCHHHHHHHCHHHHHHHCCC
HVPVGLEHGTVIVVENGEPYEVTTFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAI
CCCCCCCCCEEEEEECCCCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEE
AMTEEGEMVDLFAGQEAIQKREIVTVGNAADRFQEDALRMMRGIRFVSTLGFSLETKTKQ
EEECCCCEEEECCCHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AIETYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHH
QYNWDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDT
HCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCE
VLLYKTGIHIAEMSERVYEAMIESYDNTSVKRVQTLFEALPIKSRQEMNVTGNDLLNWAN
EEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCC
KKPGPWVAEMIQNIEEAIVQGNVVNEKECIREWLQECNLL
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA