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The map label for this gene is blyA [H]

Identifier: 118477048

GI number: 118477048

Start: 1491999

End: 1492538

Strand: Direct

Name: blyA [H]

Synonym: BALH_1349

Alternate gene names: 118477048

Gene position: 1491999-1492538 (Clockwise)

Preceding gene: 118477047

Following gene: 118477049

Centisome position: 28.38

GC content: 37.59

Gene sequence:

>540_bases
TTGGTAGTAATGGCGATGTTTCCTATAGAGCGTAACTATATTGGATATGGAAGTTCACGGCCGGGAATTCCTCTTTCTAA
AGTAAGGTTTATTGTAAGCCATGATACGGGGAATCCTGGTAGCAATGCGATTGGGAATCGGGATTATTTTAATGAGATAC
AACCGAAAGCTTCGGCACATACATTTATAGATGACAAAACAATACTAGAAATTATTCCTATAAATGAAGTTGCTTATCAT
GTTCGATATGGTGTGCCGACAGATAATGACTTATATGGGTATGATGCCAATAAGGCTGCTATTGGAGTGGAGCTTTGTTA
TGGCGGTGATGTTAATTTTTGGGAAGCATATACCCGTTTTACGTGGTATCATGCGTATTTATGTCAAAACTTCGGCTTGA
ATCCGAAAAAAGATATCGTATCACATAAAACACTTGATCCAGCCAGAAAGATTGATCCTGAAAATGTTTTAGGGAAACAA
GGTATTAAATTTCAGCAGTTTTTAGCAGATGTCTATCGGATGTACGTTTCGTTTAGATGA

Upstream 100 bases:

>100_bases
TCAAATATTTTCGATTTTCTCTTCGCGTAAGAAATATGCTATAAGGACATTGCTACCGAATACATATATAAAGAAGTAAG
GTAATCTTCTGAAGCGGGAG

Downstream 100 bases:

>100_bases
CAAATATATGAAAAATGAATACAATAAAGATAGGTGCCTACTGCTGTAAGTAGGTTTTTTCTTGTTTTTATAAATAGTGT
ATTGGTAGAAGACTAGGAGT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MVVMAMFPIERNYIGYGSSRPGIPLSKVRFIVSHDTGNPGSNAIGNRDYFNEIQPKASAHTFIDDKTILEIIPINEVAYH
VRYGVPTDNDLYGYDANKAAIGVELCYGGDVNFWEAYTRFTWYHAYLCQNFGLNPKKDIVSHKTLDPARKIDPENVLGKQ
GIKFQQFLADVYRMYVSFR

Sequences:

>Translated_179_residues
MVVMAMFPIERNYIGYGSSRPGIPLSKVRFIVSHDTGNPGSNAIGNRDYFNEIQPKASAHTFIDDKTILEIIPINEVAYH
VRYGVPTDNDLYGYDANKAAIGVELCYGGDVNFWEAYTRFTWYHAYLCQNFGLNPKKDIVSHKTLDPARKIDPENVLGKQ
GIKFQQFLADVYRMYVSFR
>Mature_179_residues
MVVMAMFPIERNYIGYGSSRPGIPLSKVRFIVSHDTGNPGSNAIGNRDYFNEIQPKASAHTFIDDKTILEIIPINEVAYH
VRYGVPTDNDLYGYDANKAAIGVELCYGGDVNFWEAYTRFTWYHAYLCQNFGLNPKKDIVSHKTLDPARKIDPENVLGKQ
GIKFQQFLADVYRMYVSFR

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Involved in prophage SP-beta-mediated cell lysis [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20414; Mature: 20414

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVMAMFPIERNYIGYGSSRPGIPLSKVRFIVSHDTGNPGSNAIGNRDYFNEIQPKASAH
CEEEEEECCCCCEECCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCE
TFIDDKTILEIIPINEVAYHVRYGVPTDNDLYGYDANKAAIGVELCYGGDVNFWEAYTRF
EEECCCEEEEEEECCCEEEEEEECCCCCCCEECCCCCCEEEEEEEEECCCCHHHHHHHHH
TWYHAYLCQNFGLNPKKDIVSHKTLDPARKIDPENVLGKQGIKFQQFLADVYRMYVSFR
HHHHHHHHHHCCCCCHHHHHCCCCCCHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVVMAMFPIERNYIGYGSSRPGIPLSKVRFIVSHDTGNPGSNAIGNRDYFNEIQPKASAH
CEEEEEECCCCCEECCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCE
TFIDDKTILEIIPINEVAYHVRYGVPTDNDLYGYDANKAAIGVELCYGGDVNFWEAYTRF
EEECCCEEEEEEECCCEEEEEEECCCCCCCEECCCCCCEEEEEEEEECCCCHHHHHHHHH
TWYHAYLCQNFGLNPKKDIVSHKTLDPARKIDPENVLGKQGIKFQQFLADVYRMYVSFR
HHHHHHHHHHCCCCCHHHHHCCCCCCHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579063; 9384377 [H]