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The map label for this gene is liaS [H]

Identifier: 118476997

GI number: 118476997

Start: 1439379

End: 1440434

Strand: Direct

Name: liaS [H]

Synonym: BALH_1295

Alternate gene names: 118476997

Gene position: 1439379-1440434 (Clockwise)

Preceding gene: 118476996

Following gene: 118476998

Centisome position: 27.38

GC content: 37.12

Gene sequence:

>1056_bases
ATGAAGAAACAAAAAAATATTTCGTGGATGTATATTCGTTATTCTATGTTGTCCTCTGTTAGTATCGCACTCATTTGTAC
AATTGTATATGTATGGAAAAGCGAGCAACAAGTGTATGATTTATTATGGAAGGAATCCATCGCTTCTGTTCCAATTGGCT
TGTTTATTATGACTACAAGTTTACTTATCGGGGGAATTGTAGGATATGCAATCGGTTACTATATAGAGCAGCGTATACAA
GGGTTAAATACGTTTCTATTTGAAGTGGAGCGTGGGAATTTTCCGAGCGATGTTTCGTTTACTGCAGATGATGAATTTCA
TGAAGTGGAACGAAAAGTAATCGTTTTGGCTCGTCGATTAGAGGAGCAAGCTGGACTTTTTCAAAAAGTAACGAATGAAC
GAGCTCATTGGAATGAAGAGATGAGACAAGAAGCGATTTCCCAAGAAAGGCATCGATTGGCGAGGGAGCTGCATGATTCT
GTAAGTCAGCAGCTTTTTGCAATGTCGATGATGATGTCGGCTATTAATGAACAAGTAGCTGAAATTCCAGAAACGACGAA
AAAGCAGTTGCAGCTTGTTGAAAATATGGTTGTAAATGCACAATCAGAGATGAGAGCATTGCTTCTTCATTTACGCCCAG
TGCAGTTAGAAGGTAAGAAACTAACAGAGGGTATAGAAGAATTATTAACAGAACTGTCTAGAAAGCAACATATGAAAATT
GAATGGTTAATTGAACCGATTCAATTAAAAAAAGGTGTAGAAGATCACTTATTCCGCATTGTACAAGAAGCGCTGTCTAA
TACTTTACGGCATGCGAAAGCAAAGAAAACGGAAGTACGTTTACGCAAAATTGAGCAATATGCAATATTGAAAATTATTG
ATGATGGTGTAGGTTTTAAAGTAGGAGTGAATAAGGCTGGTTCTTACGGATTAAGATCAATGCAAGAGCGAGTTCATGAA
ATTGGTGGTACATTAAAAGTGCTTAGTTTTCCAAATAAAGGTACACAAATAGAAGTAAAAGTACCGATTATGATTGAGAG
AGGGGGAGAATCATGA

Upstream 100 bases:

>100_bases
GTAATGTTACATTTAGAACAGAGCAGTATAAAGAGGCTCCTCGTCGTATTAAAATTATTTCTTCGCTTGTCGTAGGCGAT
ACGGAAGTGAGGAAAGTATA

Downstream 100 bases:

>100_bases
TAAAAGTATTACTAGTGGATGATCATGAAATGGTTCGAATGGGTGTATCAGCATATTTATCAACGCAGCCAGATATTGAA
GTAGTTGGAGAAGCTGAAAA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKKQKNISWMYIRYSMLSSVSIALICTIVYVWKSEQQVYDLLWKESIASVPIGLFIMTTSLLIGGIVGYAIGYYIEQRIQ
GLNTFLFEVERGNFPSDVSFTADDEFHEVERKVIVLARRLEEQAGLFQKVTNERAHWNEEMRQEAISQERHRLARELHDS
VSQQLFAMSMMMSAINEQVAEIPETTKKQLQLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMKI
EWLIEPIQLKKGVEDHLFRIVQEALSNTLRHAKAKKTEVRLRKIEQYAILKIIDDGVGFKVGVNKAGSYGLRSMQERVHE
IGGTLKVLSFPNKGTQIEVKVPIMIERGGES

Sequences:

>Translated_351_residues
MKKQKNISWMYIRYSMLSSVSIALICTIVYVWKSEQQVYDLLWKESIASVPIGLFIMTTSLLIGGIVGYAIGYYIEQRIQ
GLNTFLFEVERGNFPSDVSFTADDEFHEVERKVIVLARRLEEQAGLFQKVTNERAHWNEEMRQEAISQERHRLARELHDS
VSQQLFAMSMMMSAINEQVAEIPETTKKQLQLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMKI
EWLIEPIQLKKGVEDHLFRIVQEALSNTLRHAKAKKTEVRLRKIEQYAILKIIDDGVGFKVGVNKAGSYGLRSMQERVHE
IGGTLKVLSFPNKGTQIEVKVPIMIERGGES
>Mature_351_residues
MKKQKNISWMYIRYSMLSSVSIALICTIVYVWKSEQQVYDLLWKESIASVPIGLFIMTTSLLIGGIVGYAIGYYIEQRIQ
GLNTFLFEVERGNFPSDVSFTADDEFHEVERKVIVLARRLEEQAGLFQKVTNERAHWNEEMRQEAISQERHRLARELHDS
VSQQLFAMSMMMSAINEQVAEIPETTKKQLQLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMKI
EWLIEPIQLKKGVEDHLFRIVQEALSNTLRHAKAKKTEVRLRKIEQYAILKIIDDGVGFKVGVNKAGSYGLRSMQERVHE
IGGTLKVLSFPNKGTQIEVKVPIMIERGGES

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=203, Percent_Identity=29.064039408867, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082311, Length=196, Percent_Identity=32.6530612244898, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788812, Length=205, Percent_Identity=25.8536585365854, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40359; Mature: 40359

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQKNISWMYIRYSMLSSVSIALICTIVYVWKSEQQVYDLLWKESIASVPIGLFIMTTS
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHH
LLIGGIVGYAIGYYIEQRIQGLNTFLFEVERGNFPSDVSFTADDEFHEVERKVIVLARRL
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EEQAGLFQKVTNERAHWNEEMRQEAISQERHRLARELHDSVSQQLFAMSMMMSAINEQVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIPETTKKQLQLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMKI
HCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCH
EWLIEPIQLKKGVEDHLFRIVQEALSNTLRHAKAKKTEVRLRKIEQYAILKIIDDGVGFK
HHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VGVNKAGSYGLRSMQERVHEIGGTLKVLSFPNKGTQIEVKVPIMIERGGES
EECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEEEEECCCCC
>Mature Secondary Structure
MKKQKNISWMYIRYSMLSSVSIALICTIVYVWKSEQQVYDLLWKESIASVPIGLFIMTTS
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHH
LLIGGIVGYAIGYYIEQRIQGLNTFLFEVERGNFPSDVSFTADDEFHEVERKVIVLARRL
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EEQAGLFQKVTNERAHWNEEMRQEAISQERHRLARELHDSVSQQLFAMSMMMSAINEQVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIPETTKKQLQLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMKI
HCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCH
EWLIEPIQLKKGVEDHLFRIVQEALSNTLRHAKAKKTEVRLRKIEQYAILKIIDDGVGFK
HHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VGVNKAGSYGLRSMQERVHEIGGTLKVLSFPNKGTQIEVKVPIMIERGGES
EECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]