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The map label for this gene is alsS [H]

Identifier: 118476958

GI number: 118476958

Start: 1400417

End: 1402174

Strand: Direct

Name: alsS [H]

Synonym: BALH_1252

Alternate gene names: 118476958

Gene position: 1400417-1402174 (Clockwise)

Preceding gene: 118476957

Following gene: 118476959

Centisome position: 26.64

GC content: 39.7

Gene sequence:

>1758_bases
GTGGACGTAGGATTTTTTTTATTGATAAAAGTTTTTAAAAGGGGGCTTATGAGAACAATGTCTTCAAAAACGGAAGAGAA
ACTGGCAACCGGTGCACAGCTATTGTTAGAAGCGCTAGAAAAGGAAGGAGTAGAAGTGATTTTTGGTTATCCTGGTGGTG
CAGTTTTACCTCTTTATGATGCGCTCTATGACTGTGAAATTCCGCATATTTTAACAAGGCATGAACAAGGAGCGATTCAC
GCTGCTGAAGGGTATGCCAGAATTACTGGTAATCCAGGGGTTGTCATTGCAACGAGTGGTCCAGGTGCTACAAATGTGAT
TACGGGTCTAGCAGATGCGATGATCGATTCGTTACCACTTGTTGTATTTACAGGGCAAGTAGCAACAACGTTAATTGGAA
GTGATGCTTTCCAAGAAGCGGATATTATGGGGCTTACGATGCCGGTGACAAAACATAACTATCAAGTGCGAAAGGCTTCA
GATTTACCTCGAATTATTAAAGAAGCATTTCATATTGCTAGAACAGGAAGACCAGGCCCAGTCGTGATCGATCTTCCGAA
AGATATGGTAGTAGAGCAAGGAGAACGATGTAGTGATGTGCAAATGGATTTACCAGGATACAACCCTAATTATGAACCGA
ATCTACTGCAAATAAACAAATTATTACAAGCAATTCAGGTTGCAAACAAGCCATTAATTTTAGCTGGAGCAGGTGTATTG
CATGCGAAAGCTTCAAAAGAATTAACAAGTTTTGCTCGTAAATATGAAATTCCTGTTGTGCATACATTACTTGGTCTTGG
TGGGTTTCCACCAGATGATGAACTATTTCTAGGGATGGGAGGAATGCACGGTTCTTACACAGCTAATATGGCGTTATATG
AATGCGACTTACTCATTAATATAGGCGCTAGATTCGATGATCGTCTTACTGGAAATTTAGCTTATTTTGCGAAAGAGGCG
ACTGTCGCGCATATCGATATTGATCCAGCCGAAATCGGAAAAAATGTACCGACTGAAATTCCTATTGTTGCAAGTGCTAA
GCAAGCGCTCCAAGTATTACTTCAACCAGAAGGAAGGAAAGAAAATCATCATGAGTGGCTCTCTTTATTAAAGAATAGAA
AAGAAAAGTATCCACTCTCTTATAAACGAAATTCTGAAAGTATTAAGCCTCAATATGCAATTGATATGTTATATGAAATT
ACGAAGGGAGAAGCGATTGTAACAACAGATGTTGGGCAACATCAAATGTGGGCAGCCCAATATTATCTGCTCAAAACTCC
AGATAAATGGGTAACTTCAGGAGGACTAGGAACGATGGGATTTGGATTTCCGGCAGCAATTGGTGCACAAATTGCGAAGC
CTGATGAACTAGTTATTGCAATTGTTGGTGACGCTGGATTTCAAATGACTCTGCAAGAATTAAGTGTGTTAAAAGAACAC
TCTTTACCTGTTAAAGTATTTATTTTAAATAATGAAGCTTTAGGAATGGTAAGACAATGGCAAGATGAATTTTATAACCA
AAGATATTCGCACTCTTTACTATCGTGTCAACCAGATTTCGTTGCTCTTGCGAATGCGTATGGCATAAAAGGAGTTCGCA
TAGATGATCCACTTCTTGCGAAGAAACAATTGCAGTATGCAATTCAATTACAAGAGCCAGTTGTAATTGATTGCCGTGTA
CTTCAATCAGAAAAGGTAATGCCGATGGTTGCGCCAGGGAAAGGTGTTCACCAAATGGAGGGAGTGGAAAAAAGGTGA

Upstream 100 bases:

>100_bases
CTGATTAAAGTTTCACTTTATGGGTCACTGAGGCAAGAGTATTCTTGCAAAGAAGGGTGGTACCGCGAAATCTTTCGTCC
CTTCAGCACATAGCTGGAGT

Downstream 100 bases:

>100_bases
AGAGAATTGTTACAGCAACAGTTCGAAATCAAAGTGGTGTGTTAAACCGAATTACAGGTGTTATGACACGAAGACATTTT
AATATTGAAAGTATTTCAGT

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MDVGFFLLIKVFKRGLMRTMSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAIH
AAEGYARITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKAS
DLPRIIKEAFHIARTGRPGPVVIDLPKDMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVL
HAKASKELTSFARKYEIPVVHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAYFAKEA
TVAHIDIDPAEIGKNVPTEIPIVASAKQALQVLLQPEGRKENHHEWLSLLKNRKEKYPLSYKRNSESIKPQYAIDMLYEI
TKGEAIVTTDVGQHQMWAAQYYLLKTPDKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEH
SLPVKVFILNNEALGMVRQWQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQYAIQLQEPVVIDCRV
LQSEKVMPMVAPGKGVHQMEGVEKR

Sequences:

>Translated_585_residues
MDVGFFLLIKVFKRGLMRTMSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAIH
AAEGYARITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKAS
DLPRIIKEAFHIARTGRPGPVVIDLPKDMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVL
HAKASKELTSFARKYEIPVVHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAYFAKEA
TVAHIDIDPAEIGKNVPTEIPIVASAKQALQVLLQPEGRKENHHEWLSLLKNRKEKYPLSYKRNSESIKPQYAIDMLYEI
TKGEAIVTTDVGQHQMWAAQYYLLKTPDKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEH
SLPVKVFILNNEALGMVRQWQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQYAIQLQEPVVIDCRV
LQSEKVMPMVAPGKGVHQMEGVEKR
>Mature_585_residues
MDVGFFLLIKVFKRGLMRTMSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAIH
AAEGYARITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKAS
DLPRIIKEAFHIARTGRPGPVVIDLPKDMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVL
HAKASKELTSFARKYEIPVVHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLINIGARFDDRLTGNLAYFAKEA
TVAHIDIDPAEIGKNVPTEIPIVASAKQALQVLLQPEGRKENHHEWLSLLKNRKEKYPLSYKRNSESIKPQYAIDMLYEI
TKGEAIVTTDVGQHQMWAAQYYLLKTPDKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEH
SLPVKVFILNNEALGMVRQWQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQYAIQLQEPVVIDCRV
LQSEKVMPMVAPGKGVHQMEGVEKR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=498, Percent_Identity=25.3012048192771, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI21361361, Length=538, Percent_Identity=23.4200743494424, Blast_Score=139, Evalue=1e-32,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=45.9363957597173, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1790104, Length=566, Percent_Identity=40.8127208480565, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1786717, Length=517, Percent_Identity=35.7833655705996, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1787096, Length=544, Percent_Identity=27.7573529411765, Blast_Score=195, Evalue=8e-51,
Organism=Escherichia coli, GI1788716, Length=484, Percent_Identity=24.5867768595041, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=485, Percent_Identity=29.0721649484536, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=485, Percent_Identity=29.0721649484536, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=558, Percent_Identity=24.1935483870968, Blast_Score=135, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=573, Percent_Identity=44.6771378708551, Blast_Score=477, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6320816, Length=487, Percent_Identity=24.2299794661191, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=496, Percent_Identity=24.3951612903226, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922626, Length=565, Percent_Identity=25.8407079646018, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64200; Mature: 64200

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVGFFLLIKVFKRGLMRTMSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
ALYDCEIPHILTRHEQGAIHAAEGYARITGNPGVVIATSGPGATNVITGLADAMIDSLPL
HHHCCCCCHHHHCCCCCCEEECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCE
VVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIARTGRPGP
EEEECHHHHHHHCCCHHCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCC
VVIDLPKDMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVL
EEEECCHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEE
HAKASKELTSFARKYEIPVVHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLIN
EHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCEECCEEEEEEEEEEE
IGARFDDRLTGNLAYFAKEATVAHIDIDPAEIGKNVPTEIPIVASAKQALQVLLQPEGRK
ECCCCCCCCCCCEEEEEECCEEEEEECCHHHHCCCCCCCCCEEHHHHHHHHHHHCCCCCC
ENHHEWLSLLKNRKEKYPLSYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAAQ
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEE
YYLLKTPDKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEH
EEEEECCCHHCCCCCCCCCCCCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHC
SLPVKVFILNNEALGMVRQWQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHCCCCCEEECCCHHH
KKQLQYAIQLQEPVVIDCRVLQSEKVMPMVAPGKGVHQMEGVEKR
HHHHHHHEEECCCEEEEEEEECCCCCCEEECCCCCCHHCCCCCCC
>Mature Secondary Structure
MDVGFFLLIKVFKRGLMRTMSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
ALYDCEIPHILTRHEQGAIHAAEGYARITGNPGVVIATSGPGATNVITGLADAMIDSLPL
HHHCCCCCHHHHCCCCCCEEECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCE
VVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIARTGRPGP
EEEECHHHHHHHCCCHHCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCC
VVIDLPKDMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVL
EEEECCHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEE
HAKASKELTSFARKYEIPVVHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECDLLIN
EHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCEECCEEEEEEEEEEE
IGARFDDRLTGNLAYFAKEATVAHIDIDPAEIGKNVPTEIPIVASAKQALQVLLQPEGRK
ECCCCCCCCCCCEEEEEECCEEEEEECCHHHHCCCCCCCCCEEHHHHHHHHHHHCCCCCC
ENHHEWLSLLKNRKEKYPLSYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAAQ
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEE
YYLLKTPDKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEH
EEEEECCCHHCCCCCCCCCCCCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHC
SLPVKVFILNNEALGMVRQWQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHCCCCCEEECCCHHH
KKQLQYAIQLQEPVVIDCRVLQSEKVMPMVAPGKGVHQMEGVEKR
HHHHHHHEEECCCEEEEEEEECCCCCCEEECCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]