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The map label for this gene is queF [H]

Identifier: 118476913

GI number: 118476913

Start: 1360785

End: 1361291

Strand: Direct

Name: queF [H]

Synonym: BALH_1205

Alternate gene names: 118476913

Gene position: 1360785-1361291 (Clockwise)

Preceding gene: 118476912

Following gene: 118476915

Centisome position: 25.88

GC content: 33.53

Gene sequence:

>507_bases
ATGTTTAAAATGGCAGGAAGATTAGATGAAGATTTAAAAGATGTAACATTATTAGGAAATCAAAATACAAAGTATTTATT
TGAATACAGTCCAGAAATTTTAGAGGTATTTGATAACAATCATCCAAACCGTGATTATTTTGTTAAATTCAATTGTCCCG
AATTTACAAGCTTATGTCCGAAAACAGGCCAACCGGATTTTGCAACAATTTATATTAGCTACATTCCAGAACAAAGAATG
GTAGAGAGTAAATCTCTAAAGCTATATTTATTTAGTTTCCGCAATCACGGTGATTTTCACGAAGATTGCATGAACGTTAT
TATGAATGATTTAATTAAATTAATGGATCCACGTTACATTGAGGTATGGGGGAAATTCACACCACGTGGAGGGATTTCAA
TTGATCCTTACTGCAACTACGGTCGTCCAGGAACGAAATATGAACAAATGGCAGACTACCGCATGATGAACCATGATCTA
TATCCAGAAACAATTGACAATCGTTAA

Upstream 100 bases:

>100_bases
TGAAAAAGCAGTTAACCGTAAAGAAATGAATAATGCAAAAGTATTGCCGCAGCTTCATGCGTTAGTATGGGGAAATAAAC
GCGGAGTATAATGAGAAGGG

Downstream 100 bases:

>100_bases
TATATAATAGAAAGAAAGGATCTGTATAACATACAGATCCTTTCTTTCTATTATATTATGCATTTTGATTAATGACAGTA
TAGTTTTTATGACTAACAAC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MFKMAGRLDEDLKDVTLLGNQNTKYLFEYSPEILEVFDNNHPNRDYFVKFNCPEFTSLCPKTGQPDFATIYISYIPEQRM
VESKSLKLYLFSFRNHGDFHEDCMNVIMNDLIKLMDPRYIEVWGKFTPRGGISIDPYCNYGRPGTKYEQMADYRMMNHDL
YPETIDNR

Sequences:

>Translated_168_residues
MFKMAGRLDEDLKDVTLLGNQNTKYLFEYSPEILEVFDNNHPNRDYFVKFNCPEFTSLCPKTGQPDFATIYISYIPEQRM
VESKSLKLYLFSFRNHGDFHEDCMNVIMNDLIKLMDPRYIEVWGKFTPRGGISIDPYCNYGRPGTKYEQMADYRMMNHDL
YPETIDNR
>Mature_168_residues
MFKMAGRLDEDLKDVTLLGNQNTKYLFEYSPEILEVFDNNHPNRDYFVKFNCPEFTSLCPKTGQPDFATIYISYIPEQRM
VESKSLKLYLFSFRNHGDFHEDCMNVIMNDLIKLMDPRYIEVWGKFTPRGGISIDPYCNYGRPGTKYEQMADYRMMNHDL
YPETIDNR

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=113, Percent_Identity=33.6283185840708, Blast_Score=73, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016856
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 19935; Mature: 19935

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKMAGRLDEDLKDVTLLGNQNTKYLFEYSPEILEVFDNNHPNRDYFVKFNCPEFTSLCP
CCCCCCCCCCCCHHEEEECCCCCEEEEEECHHHHHHHCCCCCCCCEEEEECCCHHHHCCC
KTGQPDFATIYISYIPEQRMVESKSLKLYLFSFRNHGDFHEDCMNVIMNDLIKLMDPRYI
CCCCCCEEEEEEEECCHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEE
EVWGKFTPRGGISIDPYCNYGRPGTKYEQMADYRMMNHDLYPETIDNR
EEEECCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MFKMAGRLDEDLKDVTLLGNQNTKYLFEYSPEILEVFDNNHPNRDYFVKFNCPEFTSLCP
CCCCCCCCCCCCHHEEEECCCCCEEEEEECHHHHHHHCCCCCCCCEEEEECCCHHHHCCC
KTGQPDFATIYISYIPEQRMVESKSLKLYLFSFRNHGDFHEDCMNVIMNDLIKLMDPRYI
CCCCCCEEEEEEEECCHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEE
EVWGKFTPRGGISIDPYCNYGRPGTKYEQMADYRMMNHDLYPETIDNR
EEEECCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA