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The map label for this gene is malS [C]

Identifier: 118476741

GI number: 118476741

Start: 1171460

End: 1172815

Strand: Direct

Name: malS [C]

Synonym: BALH_1021

Alternate gene names: 118476741

Gene position: 1171460-1172815 (Clockwise)

Preceding gene: 118476739

Following gene: 118476742

Centisome position: 22.28

GC content: 33.41

Gene sequence:

>1356_bases
ATGCGATTCGTTCGGATAATTGGTATAATATTCTTACAATTAAAAACATGGGGGATGCGTGTGGGGAAAATACATACTAG
GAAACTATTCATTTGTTTTTGTTTAGCTGTCGTTTTGTTTGTACCAATACATACATTTGCAGACGAAAAAAGAGAGTGGC
GAGATGAAGTTATATATTCCATTATGATTGATCGTTTTAATAATGGGGAACCGAAAAATGACAAACAGTTAGAAGTTGGT
AATTTAGAAGGATATCAGGGCGGAGATATAAGAGGCATTATAAAAAGACTGGATTACATAAAAGAAATGGGATTTACCAC
TGTTATGCTTTCGCCGCTGTTTGAAAGTGTAAAATACGATGGATTAGACGTGCGCAATTTTCAGAAGGTAAATGAACATT
TCGGAACAGAAAATGATGTAAAAGAACTTGTGCAAGAAGCTCATGCAAAAGGAATGAAAGTTATATTTCAATTTCCGCTT
GGAGAAAACGAACAACAAGTAATCGACTCGATGAAATGGTGGGTCAAAGAAGTTGATTTAGATGCAAGTTATGTAATGCA
TAGTGAAAAAAAGTCTCCTGCTTTTTGGGATGATGTGCAAAAAGATATGCAAGTGATAAAAAAAGATTTTCGAGTTATGA
CAAAAGAAGATAGTGAATACAACGAAAAAATAGTAGAATCGTTTTCTAAAGCGGACGTATCGGTAAAATCTTTATATGAT
GTGAGTAAAAAAGACGAGGAATTCATTACATTTTTAGATAATCAAGATACAAAAAGATTTGCTCGTATTGCAAAGGAAAA
TATGAATTATCCGCCATCGCGTTTGAAACTAGCTCTTACATATTTATTGACATCACCAGGCATCCCGAATTTTTATTACG
GGACTGAAATTGCATTAGATGGAGGGGATACTCCAGATAATAGACGATTAATGGATTTCAAATCGGATGAAAAGTTTATG
CAGCATATAACAAAACTTGGTGAACTTAGACAAACGAGACCATCTTTACGACGCGGTACATTTGAACTCTTATACGACAA
AAATGGAATGAGTGTACTAAAACGAAAATATAAAGGTGAAGTCACATTAGTAGCGATTAATAATACGAAAGAGACGCAAA
AAGTTGCTTTACCTGCAAGTGCGATTGGTGAAAAACAAGAGTTAAGAGGATTGTTAGAAGATGAAATTATAAGAGAAGAA
AATAGAAAGTTTTATCTCGTTTTAAAGCGTGAAGAATCAAATGTGTATAAAGTTAATAGAGAAACAGGTGTGAATTGGTT
ATTTATCTCCTTAATAGTTGGTGTGAACGTATTATTTATTACTTTTTTAATTGCGGTTAAAAAGAGACGGAAATGA

Upstream 100 bases:

>100_bases
ATTCGAAGTGAAAAGAAAAATCAACTAAAAATTCAGAAAATTTTATGTGTTATGTCAGAAAATCTAACATGTATTAATTT
AATAAGAATGAAATCAACGT

Downstream 100 bases:

>100_bases
GTAACGATTTTCTTTAGTAGCTCGTCTTCAGTTTAAGGTAAGGTGTTGCTCTTAATAAGTATAGGGAGTCAGGTTAAAGA
GAAGACTTTCTCTTTAACCT

Product: alpha-amylase family protein

Products: NA

Alternate protein names: CDase; Cyclomaltodextrin hydrolase, decycling [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MRFVRIIGIIFLQLKTWGMRVGKIHTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLEVG
NLEGYQGGDIRGIIKRLDYIKEMGFTTVMLSPLFESVKYDGLDVRNFQKVNEHFGTENDVKELVQEAHAKGMKVIFQFPL
GENEQQVIDSMKWWVKEVDLDASYVMHSEKKSPAFWDDVQKDMQVIKKDFRVMTKEDSEYNEKIVESFSKADVSVKSLYD
VSKKDEEFITFLDNQDTKRFARIAKENMNYPPSRLKLALTYLLTSPGIPNFYYGTEIALDGGDTPDNRRLMDFKSDEKFM
QHITKLGELRQTRPSLRRGTFELLYDKNGMSVLKRKYKGEVTLVAINNTKETQKVALPASAIGEKQELRGLLEDEIIREE
NRKFYLVLKREESNVYKVNRETGVNWLFISLIVGVNVLFITFLIAVKKRRK

Sequences:

>Translated_451_residues
MRFVRIIGIIFLQLKTWGMRVGKIHTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLEVG
NLEGYQGGDIRGIIKRLDYIKEMGFTTVMLSPLFESVKYDGLDVRNFQKVNEHFGTENDVKELVQEAHAKGMKVIFQFPL
GENEQQVIDSMKWWVKEVDLDASYVMHSEKKSPAFWDDVQKDMQVIKKDFRVMTKEDSEYNEKIVESFSKADVSVKSLYD
VSKKDEEFITFLDNQDTKRFARIAKENMNYPPSRLKLALTYLLTSPGIPNFYYGTEIALDGGDTPDNRRLMDFKSDEKFM
QHITKLGELRQTRPSLRRGTFELLYDKNGMSVLKRKYKGEVTLVAINNTKETQKVALPASAIGEKQELRGLLEDEIIREE
NRKFYLVLKREESNVYKVNRETGVNWLFISLIVGVNVLFITFLIAVKKRRK
>Mature_451_residues
MRFVRIIGIIFLQLKTWGMRVGKIHTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLEVG
NLEGYQGGDIRGIIKRLDYIKEMGFTTVMLSPLFESVKYDGLDVRNFQKVNEHFGTENDVKELVQEAHAKGMKVIFQFPL
GENEQQVIDSMKWWVKEVDLDASYVMHSEKKSPAFWDDVQKDMQVIKKDFRVMTKEDSEYNEKIVESFSKADVSVKSLYD
VSKKDEEFITFLDNQDTKRFARIAKENMNYPPSRLKLALTYLLTSPGIPNFYYGTEIALDGGDTPDNRRLMDFKSDEKFM
QHITKLGELRQTRPSLRRGTFELLYDKNGMSVLKRKYKGEVTLVAINNTKETQKVALPASAIGEKQELRGLLEDEIIREE
NRKFYLVLKREESNVYKVNRETGVNWLFISLIVGVNVLFITFLIAVKKRRK

Specific function: Hydrolyzes cyclodextrins. Can also act on linear maltodextrins, with the exception of maltose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=128, Percent_Identity=27.34375, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786604, Length=154, Percent_Identity=33.1168831168831, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790687, Length=105, Percent_Identity=31.4285714285714, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322245, Length=104, Percent_Identity=32.6923076923077, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324416, Length=108, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322241, Length=108, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322021, Length=108, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321731, Length=108, Percent_Identity=32.4074074074074, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319776, Length=108, Percent_Identity=32.4074074074074, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321726, Length=108, Percent_Identity=32.4074074074074, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24583745, Length=162, Percent_Identity=30.8641975308642, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221330053, Length=147, Percent_Identity=30.6122448979592, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45549022, Length=179, Percent_Identity=26.2569832402235, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24586587, Length=99, Percent_Identity=38.3838383838384, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.54 [H]

Molecular weight: Translated: 52681; Mature: 52681

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644767; 8425614 [H]