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The map label for this gene is lmrB [H]

Identifier: 118476576

GI number: 118476576

Start: 980471

End: 981946

Strand: Direct

Name: lmrB [H]

Synonym: BALH_0851

Alternate gene names: 118476576

Gene position: 980471-981946 (Clockwise)

Preceding gene: 118476574

Following gene: 118476581

Centisome position: 18.65

GC content: 37.13

Gene sequence:

>1476_bases
ATGAATATGACAACTAAGAGCCCTACAAGTGGCAAAGTTGATACTTCTAATTTGAAACATACCCCCATTTTAATTGCATT
ACTTTTAGGGGCAATGGTTGCATTATTAAACGAAACATTACTTGGTAATGCGTTAACAGTATTAATGAAAGAGTTTGGTG
TAACAGCTTCAACGATTCAATGGCTTTCTACAGCTTACATGTTAGTGGTTGGGGTTTTAGTTCCGATTACAGCGTTACTT
CAGCAATGGCTCACAACGAGACAAATGTTTTTAATCGCTATGGTAACGTTTTTAGTCGGTACATTAATTGCGGGATTTGC
ACCGACATTTTCCGTTTTATTAGTCGGCCGTATCGTCCAAGCAGTAGCGACGGGATTAATTACACCGTTATTAATGAATA
CGATTTTAATTATTTGTCCGCCTGAAAAACGGGGTGCCACAATGGGGTTAATCGCACTTGTAATGATGGCAGCTCCAGCA
ATTGGGCCTACATTATCTGGAGTGATTGTGGATTCATTAAATTGGCGCTGGTTATTTTATATTGTTATTCCTGTCGTGAT
TATTTCCATTATGATTGGAATGAAGTACATTCAAAATGTTTCAGAGTTAACAAGACCGAAAGTAGATTATCCATCTATCC
TTTTATCAACATTAGGATTTGGCGGGCTTGTGTATAGCTTTAGTGCATCGGGTGATTTAGGGTGGTCTGATGCGAAAGTG
TATGGCACTTTACTTGTTGGGCTTATCTCATTATGTATTTTTGTTGCTCGACAATTAAAAATTGAGAATCCAATTTTAGA
ATTACGCGCATTTAAAGTTCCGATGTTTACATTATCAGTTGGATTAATCGTCATTGTGATGATGTCATTATTTTCAACGA
TGACTTTATTACCGATGTTCCTGCAAACAGTTTTACTCGTTACAGCCTTTAAATCAGGAATCATCATGCTTCCGGGAAGT
GTTATTAGCGCCGTAATGGGACCAATTGCAGGTAAACTATTTGATAAATTTAGTCCGAAAGTTATTATCGTTCCCGGCAT
TGTTTTAGTAGGGATTGCGATGTTCTTATTTAAAGGCATTACTCCTGATACATCAATAGTACAAATTATTGTCATGCATA
GCGTATTAATGGTTGGACTCATGTTTGTCATGACAGCACAGACATATGGCTTAAATCAATTAACACCAAATTTATATCCG
CATGGAACAGCGTTGTTTAATACTCTGCAGCAAGTAGCTGGCGCAATTGGAACAGCTATCTTTATTTCGAAAATGTCTTC
AGGAACAACTAGCTATATGGAAAGCTCCGCAAATCCAATGGATCCAGTAGAGAAGTTAAATGGTTTAACATCTGGATTCC
AAGGTGCATTTGCACTAGGGTTAATCTTTATCATCGTTGCTTTTATTGTATCGTTGTTCTTAAAAGAAGAGAAAAAGGGA
GTAGAAGCAAGGAGAGTTTTACAGAACGAAAATTAA

Upstream 100 bases:

>100_bases
ACAATTGATTCTCCCTTTTTAATCTACATATGTATATTATATTACATGTGTTCAGGTTTATTTAAAGTTTTTTAGTAAGA
AACAGGAGGAGAATTTGTAA

Downstream 100 bases:

>100_bases
GTTCCATATAAGAAGACATCTTTCAGAGGGAAAGATGTCTTTTACATTTCCTCCGTAACATCATACCGAAGCAACTCTCC
CTGATCATAAAACCTCTCAA

Product: lincomycin resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MNMTTKSPTSGKVDTSNLKHTPILIALLLGAMVALLNETLLGNALTVLMKEFGVTASTIQWLSTAYMLVVGVLVPITALL
QQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQAVATGLITPLLMNTILIICPPEKRGATMGLIALVMMAAPA
IGPTLSGVIVDSLNWRWLFYIVIPVVIISIMIGMKYIQNVSELTRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDAKV
YGTLLVGLISLCIFVARQLKIENPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMFLQTVLLVTAFKSGIIMLPGS
VISAVMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGITPDTSIVQIIVMHSVLMVGLMFVMTAQTYGLNQLTPNLYP
HGTALFNTLQQVAGAIGTAIFISKMSSGTTSYMESSANPMDPVEKLNGLTSGFQGAFALGLIFIIVAFIVSLFLKEEKKG
VEARRVLQNEN

Sequences:

>Translated_491_residues
MNMTTKSPTSGKVDTSNLKHTPILIALLLGAMVALLNETLLGNALTVLMKEFGVTASTIQWLSTAYMLVVGVLVPITALL
QQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQAVATGLITPLLMNTILIICPPEKRGATMGLIALVMMAAPA
IGPTLSGVIVDSLNWRWLFYIVIPVVIISIMIGMKYIQNVSELTRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDAKV
YGTLLVGLISLCIFVARQLKIENPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMFLQTVLLVTAFKSGIIMLPGS
VISAVMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGITPDTSIVQIIVMHSVLMVGLMFVMTAQTYGLNQLTPNLYP
HGTALFNTLQQVAGAIGTAIFISKMSSGTTSYMESSANPMDPVEKLNGLTSGFQGAFALGLIFIIVAFIVSLFLKEEKKG
VEARRVLQNEN
>Mature_491_residues
MNMTTKSPTSGKVDTSNLKHTPILIALLLGAMVALLNETLLGNALTVLMKEFGVTASTIQWLSTAYMLVVGVLVPITALL
QQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQAVATGLITPLLMNTILIICPPEKRGATMGLIALVMMAAPA
IGPTLSGVIVDSLNWRWLFYIVIPVVIISIMIGMKYIQNVSELTRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDAKV
YGTLLVGLISLCIFVARQLKIENPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMFLQTVLLVTAFKSGIIMLPGS
VISAVMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGITPDTSIVQIIVMHSVLMVGLMFVMTAQTYGLNQLTPNLYP
HGTALFNTLQQVAGAIGTAIFISKMSSGTTSYMESSANPMDPVEKLNGLTSGFQGAFALGLIFIIVAFIVSLFLKEEKKG
VEARRVLQNEN

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=430, Percent_Identity=24.4186046511628, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1788710, Length=431, Percent_Identity=24.8259860788863, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87081983, Length=333, Percent_Identity=24.6246246246246, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1790195, Length=393, Percent_Identity=24.4274809160305, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788392, Length=395, Percent_Identity=25.8227848101266, Blast_Score=82, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323735, Length=318, Percent_Identity=26.1006289308176, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=145, Percent_Identity=27.5862068965517, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=136, Percent_Identity=30.8823529411765, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=320, Percent_Identity=22.8125, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52812; Mature: 52812

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMTTKSPTSGKVDTSNLKHTPILIALLLGAMVALLNETLLGNALTVLMKEFGVTASTIQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
WLSTAYMLVVGVLVPITALLQQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVATGLITPLLMNTILIICPPEKRGATMGLIALVMMAAPAIGPTLSGVIVDSLNWRWLFY
HHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHH
IVIPVVIISIMIGMKYIQNVSELTRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDAKV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHEEECCCCCCCCCHHHH
YGTLLVGLISLCIFVARQLKIENPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMF
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQTVLLVTAFKSGIIMLPGSVISAVMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGI
HHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCC
TPDTSIVQIIVMHSVLMVGLMFVMTAQTYGLNQLTPNLYPHGTALFNTLQQVAGAIGTAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
FISKMSSGTTSYMESSANPMDPVEKLNGLTSGFQGAFALGLIFIIVAFIVSLFLKEEKKG
HHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VEARRVLQNEN
HHHHHHHCCCC
>Mature Secondary Structure
MNMTTKSPTSGKVDTSNLKHTPILIALLLGAMVALLNETLLGNALTVLMKEFGVTASTIQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
WLSTAYMLVVGVLVPITALLQQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVATGLITPLLMNTILIICPPEKRGATMGLIALVMMAAPAIGPTLSGVIVDSLNWRWLFY
HHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHH
IVIPVVIISIMIGMKYIQNVSELTRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDAKV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHEEECCCCCCCCCHHHH
YGTLLVGLISLCIFVARQLKIENPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMF
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQTVLLVTAFKSGIIMLPGSVISAVMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGI
HHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCC
TPDTSIVQIIVMHSVLMVGLMFVMTAQTYGLNQLTPNLYPHGTALFNTLQQVAGAIGTAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
FISKMSSGTTSYMESSANPMDPVEKLNGLTSGFQGAFALGLIFIIVAFIVSLFLKEEKKG
HHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VEARRVLQNEN
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]