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The map label for this gene is citS [H]

Identifier: 118476559

GI number: 118476559

Start: 952410

End: 953999

Strand: Direct

Name: citS [H]

Synonym: BALH_0831

Alternate gene names: 118476559

Gene position: 952410-953999 (Clockwise)

Preceding gene: 118476557

Following gene: 118476560

Centisome position: 18.12

GC content: 36.98

Gene sequence:

>1590_bases
ATGAAGAAATCACGCAAAGTGTCATTACAAACGAAAATAGTAAGCTTAATTATCGCGTTAATTTTATTTGTCGTTCTCCT
GTTAGCAGGAATATTTGTGTACATTCAGTCCGTTGATACGAAGCGTCAAGTAGAACAGTTAGCACTGCAAACAGCTAAAT
CTCTTTCTTTTATGCCAGCTATAAAAGAAGCTTTTCAAAATAAAGAGCATAAAAGTACGATCCAATCTATTGCAGAACAA
GTTCGTGAGCAAGCTGGTGCAGATACTGTCATTGTAGAAAATCGCTCTGGGGTTATGTATTCACATTCTAATTCTGAGTT
AATTGGAACAAAGAGTAACAATCCATATAACTATGAAGCACTTACTTTTGGCGGTTATTATACGCTCGAAGGAAATGGTG
TAAGTGGTCCGGCTTTAATGGCGAAAGCACCGATTATTGTTCATAACGGAGACTATGATCAAGTAGTAGGTGTTGTGACA
GTGGAGTTTTTAATAAAAGGCATTGAATCTAACATATTGAGCAGAACGAAAGAAATCATCCTCTTTTCCGTAGCAGTATT
ATTAGCAGGCATTGTAGGTGGCATCCTTTTAGCACGTAGCATTCGTAAAGATACACTCGGTTTAGAGCCGAATGAAATCG
CAGCGTTATACCGGGAACGAAGCGCAATACTACTATCTATAAAAGAAGGGATCATCGCCATTGATCAAAATGGTTTTATT
ACGATGATGAATACGTCGGCAGAAGAGATGCTGCATGTAAATGGTGATTATATGCAGCAACACATTTCAAAAGTTTTACC
TGAGTTTAATATGGAGAGAGTATTAGAAAACGATCAAGAAATCGCGTTTCAAGATAAAGTGTTTATTTTAAATAGGACGC
CGATACTCGAAAATAATAGTACGGTAGGCGTTGTATGTAGTTTTAGAGATAAAACAGAATTGCAAAACTTAGTGAACACG
ATATCTGAGGTAAGAAAGTATTCCGAGGACTTACGCGCTCAAACACATGAATTTACAAATAAACTTTTCGTCTTATCAGG
ATTACTTCAGCTAGGACATTACAAGGAAGCGATTGAATTTATTCAGCAAGAATCTAACATTCATCAAAGCCAAAACCATA
TTTTATTCCATCAAATTCATGATGCGAAAGTACAAGCTATTTTATTAGGCAAAATCGGAACAGCATCTGAGAAGAAAATT
GATTTTCATATTGAAGGAGATAGTGCGCTGCATCCGTTACCAGATCATATAAAAGTATCGCACCTTATTACGATTCTTGG
AAACATTATCGATAATGCGTTTGATGCGGTGAGTGGGCAAGAGGAAAAGAGTGTTTCTTTCTTTGTCACGGATATTGGGC
ACGACATTGTGTTTGAAGTGATAGATAGTGGAGCAGGCATACCGGCAGAAAAAATCACGACCATTTTTCAAAAAGGATTT
TCAACGAAAGGAAATGATCGTGGTTACGGACTAGCGAATGTGAAAGAAATGGTAGATCTACTAGAGGGAACAATTGAAAT
TCAAAACGAGAAAAATGGGGGAGCGATTTTTACAATTTACCTTCCGAAAACATTGAGAGAAAGTACATGA

Upstream 100 bases:

>100_bases
GAACATGGATATTTTTAATAGTTGCTTCATTTTCAGTTTTGTCATAATAATAGAAAAAGGAGATTGATAGTTTTGAATAT
TTTATCAAAGGATATGAAGT

Downstream 100 bases:

>100_bases
CAAACAGGGGGATGGGCATATGTTGAAGGTTGCAATTGCAGAAGATGATTTCCGCGTCGCACAAATTCAGGAAGAGTTTT
TATCAAAAATAAAGGACGTA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MKKSRKVSLQTKIVSLIIALILFVVLLLAGIFVYIQSVDTKRQVEQLALQTAKSLSFMPAIKEAFQNKEHKSTIQSIAEQ
VREQAGADTVIVENRSGVMYSHSNSELIGTKSNNPYNYEALTFGGYYTLEGNGVSGPALMAKAPIIVHNGDYDQVVGVVT
VEFLIKGIESNILSRTKEIILFSVAVLLAGIVGGILLARSIRKDTLGLEPNEIAALYRERSAILLSIKEGIIAIDQNGFI
TMMNTSAEEMLHVNGDYMQQHISKVLPEFNMERVLENDQEIAFQDKVFILNRTPILENNSTVGVVCSFRDKTELQNLVNT
ISEVRKYSEDLRAQTHEFTNKLFVLSGLLQLGHYKEAIEFIQQESNIHQSQNHILFHQIHDAKVQAILLGKIGTASEKKI
DFHIEGDSALHPLPDHIKVSHLITILGNIIDNAFDAVSGQEEKSVSFFVTDIGHDIVFEVIDSGAGIPAEKITTIFQKGF
STKGNDRGYGLANVKEMVDLLEGTIEIQNEKNGGAIFTIYLPKTLREST

Sequences:

>Translated_529_residues
MKKSRKVSLQTKIVSLIIALILFVVLLLAGIFVYIQSVDTKRQVEQLALQTAKSLSFMPAIKEAFQNKEHKSTIQSIAEQ
VREQAGADTVIVENRSGVMYSHSNSELIGTKSNNPYNYEALTFGGYYTLEGNGVSGPALMAKAPIIVHNGDYDQVVGVVT
VEFLIKGIESNILSRTKEIILFSVAVLLAGIVGGILLARSIRKDTLGLEPNEIAALYRERSAILLSIKEGIIAIDQNGFI
TMMNTSAEEMLHVNGDYMQQHISKVLPEFNMERVLENDQEIAFQDKVFILNRTPILENNSTVGVVCSFRDKTELQNLVNT
ISEVRKYSEDLRAQTHEFTNKLFVLSGLLQLGHYKEAIEFIQQESNIHQSQNHILFHQIHDAKVQAILLGKIGTASEKKI
DFHIEGDSALHPLPDHIKVSHLITILGNIIDNAFDAVSGQEEKSVSFFVTDIGHDIVFEVIDSGAGIPAEKITTIFQKGF
STKGNDRGYGLANVKEMVDLLEGTIEIQNEKNGGAIFTIYLPKTLREST
>Mature_529_residues
MKKSRKVSLQTKIVSLIIALILFVVLLLAGIFVYIQSVDTKRQVEQLALQTAKSLSFMPAIKEAFQNKEHKSTIQSIAEQ
VREQAGADTVIVENRSGVMYSHSNSELIGTKSNNPYNYEALTFGGYYTLEGNGVSGPALMAKAPIIVHNGDYDQVVGVVT
VEFLIKGIESNILSRTKEIILFSVAVLLAGIVGGILLARSIRKDTLGLEPNEIAALYRERSAILLSIKEGIIAIDQNGFI
TMMNTSAEEMLHVNGDYMQQHISKVLPEFNMERVLENDQEIAFQDKVFILNRTPILENNSTVGVVCSFRDKTELQNLVNT
ISEVRKYSEDLRAQTHEFTNKLFVLSGLLQLGHYKEAIEFIQQESNIHQSQNHILFHQIHDAKVQAILLGKIGTASEKKI
DFHIEGDSALHPLPDHIKVSHLITILGNIIDNAFDAVSGQEEKSVSFFVTDIGHDIVFEVIDSGAGIPAEKITTIFQKGF
STKGNDRGYGLANVKEMVDLLEGTIEIQNEKNGGAIFTIYLPKTLREST

Specific function: Member of the two-component regulatory system CitT/CitS. Regulates the expression of the citM-yflN operon. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=532, Percent_Identity=31.015037593985, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI1786837, Length=537, Percent_Identity=30.1675977653631, Blast_Score=233, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58628; Mature: 58628

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSRKVSLQTKIVSLIIALILFVVLLLAGIFVYIQSVDTKRQVEQLALQTAKSLSFMPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IKEAFQNKEHKSTIQSIAEQVREQAGADTVIVENRSGVMYSHSNSELIGTKSNNPYNYEA
HHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCEEEECCCCCCCEEE
LTFGGYYTLEGNGVSGPALMAKAPIIVHNGDYDQVVGVVTVEFLIKGIESNILSRTKEII
EEECCEEEEECCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFSVAVLLAGIVGGILLARSIRKDTLGLEPNEIAALYRERSAILLSIKEGIIAIDQNGFI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCEEEEECCCEE
TMMNTSAEEMLHVNGDYMQQHISKVLPEFNMERVLENDQEIAFQDKVFILNRTPILENNS
EEECCCHHHHEECCHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECEEEEEECCCEEECCC
TVGVVCSFRDKTELQNLVNTISEVRKYSEDLRAQTHEFTNKLFVLSGLLQLGHYKEAIEF
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQQESNIHQSQNHILFHQIHDAKVQAILLGKIGTASEKKIDFHIEGDSALHPLPDHIKVS
HHHHHCCHHCCCCEEEEEECCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHH
HLITILGNIIDNAFDAVSGQEEKSVSFFVTDIGHDIVFEVIDSGAGIPAEKITTIFQKGF
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
STKGNDRGYGLANVKEMVDLLEGTIEIQNEKNGGAIFTIYLPKTLREST
CCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCHHHCCCC
>Mature Secondary Structure
MKKSRKVSLQTKIVSLIIALILFVVLLLAGIFVYIQSVDTKRQVEQLALQTAKSLSFMPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IKEAFQNKEHKSTIQSIAEQVREQAGADTVIVENRSGVMYSHSNSELIGTKSNNPYNYEA
HHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCEEEECCCCCCCEEE
LTFGGYYTLEGNGVSGPALMAKAPIIVHNGDYDQVVGVVTVEFLIKGIESNILSRTKEII
EEECCEEEEECCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFSVAVLLAGIVGGILLARSIRKDTLGLEPNEIAALYRERSAILLSIKEGIIAIDQNGFI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCEEEEECCCEE
TMMNTSAEEMLHVNGDYMQQHISKVLPEFNMERVLENDQEIAFQDKVFILNRTPILENNS
EEECCCHHHHEECCHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECEEEEEECCCEEECCC
TVGVVCSFRDKTELQNLVNTISEVRKYSEDLRAQTHEFTNKLFVLSGLLQLGHYKEAIEF
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQQESNIHQSQNHILFHQIHDAKVQAILLGKIGTASEKKIDFHIEGDSALHPLPDHIKVS
HHHHHCCHHCCCCEEEEEECCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHH
HLITILGNIIDNAFDAVSGQEEKSVSFFVTDIGHDIVFEVIDSGAGIPAEKITTIFQKGF
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
STKGNDRGYGLANVKEMVDLLEGTIEIQNEKNGGAIFTIYLPKTLREST
CCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 10972810 [H]