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The map label for this gene is cls [H]

Identifier: 118476308

GI number: 118476308

Start: 652152

End: 653681

Strand: Reverse

Name: cls [H]

Synonym: BALH_0563

Alternate gene names: 118476308

Gene position: 653681-652152 (Counterclockwise)

Preceding gene: 118476311

Following gene: 118476307

Centisome position: 12.43

GC content: 34.58

Gene sequence:

>1530_bases
ATGAAGAAACCTATCGTCCAATTACTATTAATATTTACAATTGTATCTATCGTTCTTTTCCTATTAAATACATCTTATAT
TTCGTTATATACATTTGTCGGCGTTCTCTGGTCCATCACAATTGTAGGAATTTCATTCGTTATTTTTATCGAAAATAGAT
CTCCTCAAAGTACCTTAGCCTGGTTTTTAGTATTAGCACTTCTTCCCGTTATAGGTGTGCTTTTATACTCTATTTTCGGG
CGTAGCCGTTGGAGAAGAAAAAAGCATCTCCATCGTTCAGAAGAACAAAGAAAGTTATTCCGTGAAATATTAGAAGGGAG
ACGACTAGAGTTATCACTCAAAGTCCCATTAAATGAACGCTCTGTCCATTTAACAGAAGTCGTACAAAAATTTGGAGGCG
GGCCTGCAGCAGATAGAACAACTACAAAGCTTTTAACAAACGGAGATCAAACATTCTCAGAAATTTTGCAAGCGATCGAG
CAAGCAAAACATCATATACATATTCAATACTATATTTATAAATCTGATGAGATTGGTACAAAAGTTCGGGATGCGTTAAT
AAAAAAAGCAAAATCTGGTGTTATTGTACGATTCCTTTATGATGGACTCGGAAGTAATACGTTAAGAAGACGGTTTTTAC
AACCTATGAAAGAAGCTGGAATTGAAATTGTTGAATTTGATCCTATTTTTTCAGCTTGGTTACTTGAAACCGTCAATTAT
CGTAACCACCGTAAAATCGTCATTGTAGATGGAGAAATCGGTTTTACTGGTGGACTAAACGTCGGTGATGAATATCTTGG
TCGTTCAAAAAAATTTCCCGTTTGGCGTGACAGTCATTTAAAAATAGAAGGAAAAGCACTATATAAATTACAAGCAATCT
TTCTAGAGGATTGGCTCTATGCATCTAGTGGATTGAATACTTATTCTTGGGATCCATTTATGAATAGACAATACTTCCCT
GGTAAAGAAATTTCAAGTGCAGAAGGTGCTGTGCAAATTGTAGCGAGCGGACCAAGCTCAGATGATAAAAGTATTCGTAA
TACGTTATTAGCTGTTATGGGATCTGCAAAAAAATCGATTTGGATTGCTACACCATACTTTATTCCAGATCAAGAAACTT
TAACCTTATTACGTTTAAGTGCAATATCTGGCATAGATGTACGCATTTTATATCCTGGTAAAAGTGATAGTATTATTAGT
GACCAAGCATCTCAGTCCTACTTCACTCCACTTTTAAAAGCTGGTGCCTCCATTTATAGTTATAAAGATGGTTTTATGCA
TGCAAAAATTGTACTTGTTGATGATAAAATTGCAACAATCGGAACAGCAAATATGGACGTACGCAGCTTTGAATTGAATT
ACGAAATTATTAGCGTACTGTATGAATCAGAAACTGTTCATGATATTAAACGTGATTTTGAAGATGACTTTAAGCATTCT
ACTGAAATTAAATGGAACGCTTTCCAAAAGAGAAGTATCAAAAAACGAATATTAGAATCTTTTATGAGGCTCATTTCTCC
TTTACTATAA

Upstream 100 bases:

>100_bases
TTTTGTAAAAAAAACAAAACACTTTATAATAAGAATGTCGGATTGTACCTTCTTATATGAAAAGTCTATATTTAAGAAAG
ATTAGAGGAATTATATAGAA

Downstream 100 bases:

>100_bases
GCAATCAATGTATTATTGATTGCTTTTTCTTTTATATTTGTCCCCTCTCCTTTTCTTTGCATACCATATTAAAACAAGTT
ATCATAAGATGTATTACTTT

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 1 [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MKKPIVQLLLIFTIVSIVLFLLNTSYISLYTFVGVLWSITIVGISFVIFIENRSPQSTLAWFLVLALLPVIGVLLYSIFG
RSRWRRKKHLHRSEEQRKLFREILEGRRLELSLKVPLNERSVHLTEVVQKFGGGPAADRTTTKLLTNGDQTFSEILQAIE
QAKHHIHIQYYIYKSDEIGTKVRDALIKKAKSGVIVRFLYDGLGSNTLRRRFLQPMKEAGIEIVEFDPIFSAWLLETVNY
RNHRKIVIVDGEIGFTGGLNVGDEYLGRSKKFPVWRDSHLKIEGKALYKLQAIFLEDWLYASSGLNTYSWDPFMNRQYFP
GKEISSAEGAVQIVASGPSSDDKSIRNTLLAVMGSAKKSIWIATPYFIPDQETLTLLRLSAISGIDVRILYPGKSDSIIS
DQASQSYFTPLLKAGASIYSYKDGFMHAKIVLVDDKIATIGTANMDVRSFELNYEIISVLYESETVHDIKRDFEDDFKHS
TEIKWNAFQKRSIKKRILESFMRLISPLL

Sequences:

>Translated_509_residues
MKKPIVQLLLIFTIVSIVLFLLNTSYISLYTFVGVLWSITIVGISFVIFIENRSPQSTLAWFLVLALLPVIGVLLYSIFG
RSRWRRKKHLHRSEEQRKLFREILEGRRLELSLKVPLNERSVHLTEVVQKFGGGPAADRTTTKLLTNGDQTFSEILQAIE
QAKHHIHIQYYIYKSDEIGTKVRDALIKKAKSGVIVRFLYDGLGSNTLRRRFLQPMKEAGIEIVEFDPIFSAWLLETVNY
RNHRKIVIVDGEIGFTGGLNVGDEYLGRSKKFPVWRDSHLKIEGKALYKLQAIFLEDWLYASSGLNTYSWDPFMNRQYFP
GKEISSAEGAVQIVASGPSSDDKSIRNTLLAVMGSAKKSIWIATPYFIPDQETLTLLRLSAISGIDVRILYPGKSDSIIS
DQASQSYFTPLLKAGASIYSYKDGFMHAKIVLVDDKIATIGTANMDVRSFELNYEIISVLYESETVHDIKRDFEDDFKHS
TEIKWNAFQKRSIKKRILESFMRLISPLL
>Mature_509_residues
MKKPIVQLLLIFTIVSIVLFLLNTSYISLYTFVGVLWSITIVGISFVIFIENRSPQSTLAWFLVLALLPVIGVLLYSIFG
RSRWRRKKHLHRSEEQRKLFREILEGRRLELSLKVPLNERSVHLTEVVQKFGGGPAADRTTTKLLTNGDQTFSEILQAIE
QAKHHIHIQYYIYKSDEIGTKVRDALIKKAKSGVIVRFLYDGLGSNTLRRRFLQPMKEAGIEIVEFDPIFSAWLLETVNY
RNHRKIVIVDGEIGFTGGLNVGDEYLGRSKKFPVWRDSHLKIEGKALYKLQAIFLEDWLYASSGLNTYSWDPFMNRQYFP
GKEISSAEGAVQIVASGPSSDDKSIRNTLLAVMGSAKKSIWIATPYFIPDQETLTLLRLSAISGIDVRILYPGKSDSIIS
DQASQSYFTPLLKAGASIYSYKDGFMHAKIVLVDDKIATIGTANMDVRSFELNYEIISVLYESETVHDIKRDFEDDFKHS
TEIKWNAFQKRSIKKRILESFMRLISPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=497, Percent_Identity=27.7665995975855, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1787007, Length=330, Percent_Identity=27.8787878787879, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI87081826, Length=381, Percent_Identity=26.7716535433071, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 58096; Mature: 58096

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPIVQLLLIFTIVSIVLFLLNTSYISLYTFVGVLWSITIVGISFVIFIENRSPQSTLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHH
WFLVLALLPVIGVLLYSIFGRSRWRRKKHLHRSEEQRKLFREILEGRRLELSLKVPLNER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCC
SVHLTEVVQKFGGGPAADRTTTKLLTNGDQTFSEILQAIEQAKHHIHIQYYIYKSDEIGT
CCHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCH
KVRDALIKKAKSGVIVRFLYDGLGSNTLRRRFLQPMKEAGIEIVEFDPIFSAWLLETVNY
HHHHHHHHHHCCCEEEEEEHHCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCC
RNHRKIVIVDGEIGFTGGLNVGDEYLGRSKKFPVWRDSHLKIEGKALYKLQAIFLEDWLY
CCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHH
ASSGLNTYSWDPFMNRQYFPGKEISSAEGAVQIVASGPSSDDKSIRNTLLAVMGSAKKSI
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEE
WIATPYFIPDQETLTLLRLSAISGIDVRILYPGKSDSIISDQASQSYFTPLLKAGASIYS
EEECCEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHEE
YKDGFMHAKIVLVDDKIATIGTANMDVRSFELNYEIISVLYESETVHDIKRDFEDDFKHS
ECCCCEEEEEEEEECCEEEEECCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHCCC
TEIKWNAFQKRSIKKRILESFMRLISPLL
CCCEEHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKPIVQLLLIFTIVSIVLFLLNTSYISLYTFVGVLWSITIVGISFVIFIENRSPQSTLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHH
WFLVLALLPVIGVLLYSIFGRSRWRRKKHLHRSEEQRKLFREILEGRRLELSLKVPLNER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCC
SVHLTEVVQKFGGGPAADRTTTKLLTNGDQTFSEILQAIEQAKHHIHIQYYIYKSDEIGT
CCHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCH
KVRDALIKKAKSGVIVRFLYDGLGSNTLRRRFLQPMKEAGIEIVEFDPIFSAWLLETVNY
HHHHHHHHHHCCCEEEEEEHHCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCC
RNHRKIVIVDGEIGFTGGLNVGDEYLGRSKKFPVWRDSHLKIEGKALYKLQAIFLEDWLY
CCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHH
ASSGLNTYSWDPFMNRQYFPGKEISSAEGAVQIVASGPSSDDKSIRNTLLAVMGSAKKSI
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEE
WIATPYFIPDQETLTLLRLSAISGIDVRILYPGKSDSIISDQASQSYFTPLLKAGASIYS
EEECCEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHEE
YKDGFMHAKIVLVDDKIATIGTANMDVRSFELNYEIISVLYESETVHDIKRDFEDDFKHS
ECCCCEEEEEEEEECCEEEEECCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHCCC
TEIKWNAFQKRSIKKRILESFMRLISPLL
CCCEEHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]