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The map label for this gene is gltX [H]

Identifier: 118475858

GI number: 118475858

Start: 103014

End: 104504

Strand: Direct

Name: gltX [H]

Synonym: BALH_0087

Alternate gene names: 118475858

Gene position: 103014-104504 (Clockwise)

Preceding gene: 118475857

Following gene: 118475859

Centisome position: 1.96

GC content: 37.16

Gene sequence:

>1491_bases
GTGTTGACATTAAGAATGGAAGGTGTACCAATTATGGAAAAGCAAGTGAGAGTGCGCTATGCGCCAAGTCCAACAGGACA
CTTACATATCGGAAATGCGCGTACGGCATTATTTAATTATTTATTTGCTCGTCATCAAGATGGTAAGTTTATTATTCGTA
TTGAAGATACTGATGTAAAACGTAATGTTGCTGGTGGAGAAGAAAGCCAATTAAAATACTTGAAATGGCTCGGTATGGAC
TGGGATGAAGGTGTTGATGTTGGTGGTGAATTTGGACCATATCGTCAAACAGAGCGTTTAGATATTTATAAAAAGTTATA
TGAAGATTTATTAGAGCGTGGTTTAGCTTACAAATGTTATATGACAGAAGAAGAGCTAGAGGCAGAACGCGAAGGGCAAA
TTGCTCGTGGTGAAACACCTCGTTACGCAGGTAACCACCGTGATTTAACTGAAGCGCAAGTGAAAGAATTTGAAGCTGAG
GGACGTATTCCAAGTATTCGTTTCCGCGTACCAGCTGACCGTGATTACACATTTAAAGATATTGTAAAAGATGAAGTTGC
ATTCCATTCAAATGATTTCGGTGATTTCGTTATCGTGAAAAAAGATGGGATTCCAACTTATAACTTTGCAGTAGCAGTAG
ATGATCACTTAATGGAAATTACACACGTACTTCGTGGTGATGACCATATTTCAAACACGCCAAAACAAATGATGATTTAT
GAAGCTTTCGGTTGGGATATTCCGCAATTCGGTCATATGACTTTAATTGTAAATGAAAGCCGTAAAAAATTAAGTAAGCG
TGATGAATCTATTATTCAATTTATTGAGCAATATAAAGAGCTTGGATATCTTCCAGAAGCAATCTTTAACTTTATTGCAC
TACTAGGTTGGTCGCCAGTAGGAGAAGAAGAAATCTTCTCTCAAGAAGAGTTTATCAAAATGTTTGATGCAGCTCGTTTA
TCAAAATCACCTGCATTATTTGATTCTCAAAAACTAAAATGGATGAACAACCAATATATGAAAAAGCAAGATTTAGATAC
GGTGGTAGAATTAAGCTTACCGCATTTAGTGAAGGCTGGACGTATAGGTGAGACTTTAAGTGAACAAGAACAAGCTTGGA
TTCGTGATGTAATTGCGTTATATCATGAACAAATGAGCTTTGGAGCTGAAATTGTAGAGCTTTCTGAAATGTTCTTCAAA
GATCACGTTGATTATGAAGAAGAAGGACAAGAAGTATTAAAAGGTGAACAAGTACCAGAAGTACTTCGTGCATTTGCTGG
TCAAGTAGAAGCACTAGAAGCTATGGAACCGGCAGCAATTAAGGCGGCTATTAAAGCGGTTCAAAAGGAAACAGGTCATA
AAGGTAAAAACTTATTTATGCCAATCCGTGTTGCAACTACTGGTCAAACACATGGCCCAGAGCTTCCTAATGCTATTGCA
CTTCTTGGAAAAGAAAAAGTTTTAAATCGTCTTCAAAAAGTAATTGGTTAA

Upstream 100 bases:

>100_bases
AAGGAATTGCTTCTCAAGCAGTTGTTTTATTACAGAAAAAATAATGGTTTTTAATTCGTAAGATGGATAATCACATTTTT
TTGGTGTACAATTATAGAAT

Downstream 100 bases:

>100_bases
CATTTTCTAGGTTTAGAAATATAATAAGTATACTTAAAACCTAATAAGGAAAAGCGACGAGAAGGAGAAGTAGAAAATCA
GGCTTTTTACAGAGAGAACC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MLTLRMEGVPIMEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHQDGKFIIRIEDTDVKRNVAGGEESQLKYLKWLGMD
WDEGVDVGGEFGPYRQTERLDIYKKLYEDLLERGLAYKCYMTEEELEAEREGQIARGETPRYAGNHRDLTEAQVKEFEAE
GRIPSIRFRVPADRDYTFKDIVKDEVAFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIY
EAFGWDIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEAIFNFIALLGWSPVGEEEIFSQEEFIKMFDAARL
SKSPALFDSQKLKWMNNQYMKKQDLDTVVELSLPHLVKAGRIGETLSEQEQAWIRDVIALYHEQMSFGAEIVELSEMFFK
DHVDYEEEGQEVLKGEQVPEVLRAFAGQVEALEAMEPAAIKAAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIA
LLGKEKVLNRLQKVIG

Sequences:

>Translated_496_residues
MLTLRMEGVPIMEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHQDGKFIIRIEDTDVKRNVAGGEESQLKYLKWLGMD
WDEGVDVGGEFGPYRQTERLDIYKKLYEDLLERGLAYKCYMTEEELEAEREGQIARGETPRYAGNHRDLTEAQVKEFEAE
GRIPSIRFRVPADRDYTFKDIVKDEVAFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIY
EAFGWDIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEAIFNFIALLGWSPVGEEEIFSQEEFIKMFDAARL
SKSPALFDSQKLKWMNNQYMKKQDLDTVVELSLPHLVKAGRIGETLSEQEQAWIRDVIALYHEQMSFGAEIVELSEMFFK
DHVDYEEEGQEVLKGEQVPEVLRAFAGQVEALEAMEPAAIKAAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIA
LLGKEKVLNRLQKVIG
>Mature_496_residues
MLTLRMEGVPIMEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHQDGKFIIRIEDTDVKRNVAGGEESQLKYLKWLGMD
WDEGVDVGGEFGPYRQTERLDIYKKLYEDLLERGLAYKCYMTEEELEAEREGQIARGETPRYAGNHRDLTEAQVKEFEAE
GRIPSIRFRVPADRDYTFKDIVKDEVAFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIY
EAFGWDIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEAIFNFIALLGWSPVGEEEIFSQEEFIKMFDAARL
SKSPALFDSQKLKWMNNQYMKKQDLDTVVELSLPHLVKAGRIGETLSEQEQAWIRDVIALYHEQMSFGAEIVELSEMFFK
DHVDYEEEGQEVLKGEQVPEVLRAFAGQVEALEAMEPAAIKAAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIA
LLGKEKVLNRLQKVIG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=335, Percent_Identity=39.4029850746269, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI4826960, Length=333, Percent_Identity=24.024024024024, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1788743, Length=481, Percent_Identity=35.5509355509356, Blast_Score=321, Evalue=6e-89,
Organism=Escherichia coli, GI226510925, Length=255, Percent_Identity=33.7254901960784, Blast_Score=135, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI71993394, Length=500, Percent_Identity=32.4, Blast_Score=241, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6324540, Length=505, Percent_Identity=33.8613861386139, Blast_Score=252, Evalue=8e-68,
Organism=Saccharomyces cerevisiae, GI6324742, Length=325, Percent_Identity=26.1538461538462, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321192, Length=220, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=33.739837398374, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI17864488, Length=310, Percent_Identity=24.8387096774194, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 56859; Mature: 56859

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLRMEGVPIMEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHQDGKFIIRIEDTDVK
CEEEEECCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCH
RNVAGGEESQLKYLKWLGMDWDEGVDVGGEFGPYRQTERLDIYKKLYEDLLERGLAYKCY
HCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
MTEEELEAEREGQIARGETPRYAGNHRDLTEAQVKEFEAEGRIPSIRFRVPADRDYTFKD
ECHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
IVKDEVAFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIY
HHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCHHEEEE
EAFGWDIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEAIFNFIALLGWSPV
HHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
GEEEIFSQEEFIKMFDAARLSKSPALFDSQKLKWMNNQYMKKQDLDTVVELSLPHLVKAG
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RIGETLSEQEQAWIRDVIALYHEQMSFGAEIVELSEMFFKDHVDYEEEGQEVLKGEQVPE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHH
VLRAFAGQVEALEAMEPAAIKAAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIA
HHHHHHCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHH
LLGKEKVLNRLQKVIG
HHHHHHHHHHHHHHHC
>Mature Secondary Structure
MLTLRMEGVPIMEKQVRVRYAPSPTGHLHIGNARTALFNYLFARHQDGKFIIRIEDTDVK
CEEEEECCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCH
RNVAGGEESQLKYLKWLGMDWDEGVDVGGEFGPYRQTERLDIYKKLYEDLLERGLAYKCY
HCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
MTEEELEAEREGQIARGETPRYAGNHRDLTEAQVKEFEAEGRIPSIRFRVPADRDYTFKD
ECHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
IVKDEVAFHSNDFGDFVIVKKDGIPTYNFAVAVDDHLMEITHVLRGDDHISNTPKQMMIY
HHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCHHEEEE
EAFGWDIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEAIFNFIALLGWSPV
HHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
GEEEIFSQEEFIKMFDAARLSKSPALFDSQKLKWMNNQYMKKQDLDTVVELSLPHLVKAG
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RIGETLSEQEQAWIRDVIALYHEQMSFGAEIVELSEMFFKDHVDYEEEGQEVLKGEQVPE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHH
VLRAFAGQVEALEAMEPAAIKAAIKAVQKETGHKGKNLFMPIRVATTGQTHGPELPNAIA
HHHHHHCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHH
LLGKEKVLNRLQKVIG
HHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA