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The map label for this gene is cysK [H]

Identifier: 118475839

GI number: 118475839

Start: 73497

End: 74438

Strand: Direct

Name: cysK [H]

Synonym: BALH_0066

Alternate gene names: 118475839

Gene position: 73497-74438 (Clockwise)

Preceding gene: 118475838

Following gene: 118475840

Centisome position: 1.4

GC content: 39.92

Gene sequence:

>942_bases
GTGTTAGGAGTGACAGGAATGCGAGTGGCACAATCAGTTTCAGAATTAATCGGGAAAACGCCGATCGTTAAGTTGAACCG
CATCGTAGAATCAGACAGCGCAGATATATACTTAAAATTAGAATTTATGAATCCGGGGAGCAGCGTTAAAGATCGTATCG
CATTAGCTATGATTGAAGATGCTGAGAAAAAAGGATTATTAAAAGAAGGCGATACAATCATTGAGCCGACAAGTGGTAAC
ACAGGAATTGGTTTAGCGATGGTAGCAGCTGCTAAAGGATATAAGGCAATTTTAGTAATGCCAGAAACAATGAGCGTTGA
GCGTCGCAATTTATTACGTGCTTATGGCGCTGAATTAGTATTGACTCCAGGGCCTGAAGGAATGGGCGGAGCAATTCGTC
AAGCGACTGAATTAGCAAAAGAACATGGCTACTTTATACCGCAACAGTTCAAAAATCAATCAAATCCAGAAATTCATCGT
TTAACAACAGGTCCAGAAATTGTTGAGCAAATGGGTGATCAATTAGATGCATTTATTGCAGGTATTGGTACAGGTGGAAC
GATTACTGGTGCTGGTGAAGTGCTGAAGGAAGCATATAAAGATATTAAAATTTATGCAGTAGAACCTGCGGATTCACCGG
TGTTATCTGGTGGAAAACCGGGTCCACATAAAATCCAAGGAATTGGGGCTGGATTTGTTCCAGAAACATTGGATGTAGAA
GTATATGATGAAATCATTCAAGTGAAAACAGAGCAAGCGTTCGAATACGCAAGAAGAGTGGCTAAAGAGGAAGGTATTTT
AGTTGGTATCTCTTCAGGAGCGGTTATTTATGCAGCGACAGAAGTTGCGAAAAAGTTAGGTAAAGGGAAAAAGGTACTTG
TTATTATCCCAAGTAATGGTGAACGTTATTTAAGTACACCACTTTATCAATTTGAGTCTTAA

Upstream 100 bases:

>100_bases
TTGAGAATTTGTAATAAGGGAATTTACCCTCTAAATACATTGACAAACAGGGAATTTTCTGACAAAATCTAAATATAGAC
AAAACCAATAAAAATACTCG

Downstream 100 bases:

>100_bases
TTACATGTGATTGAAAAAGCATCCTTGAAAAAGGATGCTTTTTCTTTTTGGATTGGAAATAGGAAAAGAGTGAATGACTA
GAAAACTTCATGTAAAATAA

Product: cysteine synthase

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; Superoxide-inducible protein 11; SOI11 [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLGVTGMRVAQSVSELIGKTPIVKLNRIVESDSADIYLKLEFMNPGSSVKDRIALAMIEDAEKKGLLKEGDTIIEPTSGN
TGIGLAMVAAAKGYKAILVMPETMSVERRNLLRAYGAELVLTPGPEGMGGAIRQATELAKEHGYFIPQQFKNQSNPEIHR
LTTGPEIVEQMGDQLDAFIAGIGTGGTITGAGEVLKEAYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPETLDVE
VYDEIIQVKTEQAFEYARRVAKEEGILVGISSGAVIYAATEVAKKLGKGKKVLVIIPSNGERYLSTPLYQFES

Sequences:

>Translated_313_residues
MLGVTGMRVAQSVSELIGKTPIVKLNRIVESDSADIYLKLEFMNPGSSVKDRIALAMIEDAEKKGLLKEGDTIIEPTSGN
TGIGLAMVAAAKGYKAILVMPETMSVERRNLLRAYGAELVLTPGPEGMGGAIRQATELAKEHGYFIPQQFKNQSNPEIHR
LTTGPEIVEQMGDQLDAFIAGIGTGGTITGAGEVLKEAYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPETLDVE
VYDEIIQVKTEQAFEYARRVAKEEGILVGISSGAVIYAATEVAKKLGKGKKVLVIIPSNGERYLSTPLYQFES
>Mature_313_residues
MLGVTGMRVAQSVSELIGKTPIVKLNRIVESDSADIYLKLEFMNPGSSVKDRIALAMIEDAEKKGLLKEGDTIIEPTSGN
TGIGLAMVAAAKGYKAILVMPETMSVERRNLLRAYGAELVLTPGPEGMGGAIRQATELAKEHGYFIPQQFKNQSNPEIHR
LTTGPEIVEQMGDQLDAFIAGIGTGGTITGAGEVLKEAYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPETLDVE
VYDEIIQVKTEQAFEYARRVAKEEGILVGISSGAVIYAATEVAKKLGKGKKVLVIIPSNGERYLSTPLYQFES

Specific function: Catalyzes the conversion of O-acetylserine to cysteine. Also acts as a sensor of cysteine availability in the signal transduction pathway modulating CymR activity. When cysteine is present, the pool of O-acetylserine (OAS) is low, which leads to the forma

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=303, Percent_Identity=43.5643564356436, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI295821200, Length=303, Percent_Identity=43.5643564356436, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI4557415, Length=303, Percent_Identity=43.5643564356436, Blast_Score=238, Evalue=4e-63,
Organism=Escherichia coli, GI1788754, Length=313, Percent_Identity=52.7156549520767, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI2367138, Length=298, Percent_Identity=41.6107382550336, Blast_Score=228, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17535051, Length=298, Percent_Identity=48.993288590604, Blast_Score=277, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI115535073, Length=305, Percent_Identity=49.8360655737705, Blast_Score=266, Evalue=8e-72,
Organism=Caenorhabditis elegans, GI17562970, Length=305, Percent_Identity=47.8688524590164, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI32566674, Length=299, Percent_Identity=48.1605351170569, Blast_Score=261, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17561720, Length=299, Percent_Identity=46.8227424749164, Blast_Score=235, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI32566672, Length=213, Percent_Identity=48.3568075117371, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17534315, Length=317, Percent_Identity=39.1167192429022, Blast_Score=175, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25147552, Length=315, Percent_Identity=36.8253968253968, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17561716, Length=117, Percent_Identity=47.008547008547, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321594, Length=318, Percent_Identity=38.9937106918239, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321449, Length=329, Percent_Identity=35.258358662614, Blast_Score=148, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24643623, Length=312, Percent_Identity=41.9871794871795, Blast_Score=214, Evalue=5e-56,
Organism=Drosophila melanogaster, GI20129101, Length=312, Percent_Identity=41.9871794871795, Blast_Score=214, Evalue=5e-56,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33478; Mature: 33478

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGVTGMRVAQSVSELIGKTPIVKLNRIVESDSADIYLKLEFMNPGSSVKDRIALAMIED
CCCCCHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHH
AEKKGLLKEGDTIIEPTSGNTGIGLAMVAAAKGYKAILVMPETMSVERRNLLRAYGAELV
HHHCCCCCCCCEEEECCCCCCCCHHHEEECCCCCEEEEEECCCCCHHHHHHHHHCCCEEE
LTPGPEGMGGAIRQATELAKEHGYFIPQQFKNQSNPEIHRLTTGPEIVEQMGDQLDAFIA
ECCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
GIGTGGTITGAGEVLKEAYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPETLDVE
CCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHH
VYDEIIQVKTEQAFEYARRVAKEEGILVGISSGAVIYAATEVAKKLGKGKKVLVIIPSNG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEHHHHHHHHCCCCEEEEEECCCC
ERYLSTPLYQFES
CCEECCCCHHCCC
>Mature Secondary Structure
MLGVTGMRVAQSVSELIGKTPIVKLNRIVESDSADIYLKLEFMNPGSSVKDRIALAMIED
CCCCCHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHH
AEKKGLLKEGDTIIEPTSGNTGIGLAMVAAAKGYKAILVMPETMSVERRNLLRAYGAELV
HHHCCCCCCCCEEEECCCCCCCCHHHEEECCCCCEEEEEECCCCCHHHHHHHHHCCCEEE
LTPGPEGMGGAIRQATELAKEHGYFIPQQFKNQSNPEIHRLTTGPEIVEQMGDQLDAFIA
ECCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
GIGTGGTITGAGEVLKEAYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPETLDVE
CCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHH
VYDEIIQVKTEQAFEYARRVAKEEGILVGISSGAVIYAATEVAKKLGKGKKVLVIIPSNG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEHHHHHHHHCCCCEEEEEECCCC
ERYLSTPLYQFES
CCEECCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8755892; 9298659 [H]