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The map label for this gene is pncA [H]

Identifier: 118475794

GI number: 118475794

Start: 23895

End: 24434

Strand: Reverse

Name: pncA [H]

Synonym: BALH_0017

Alternate gene names: 118475794

Gene position: 24434-23895 (Counterclockwise)

Preceding gene: 118475809

Following gene: 118475793

Centisome position: 0.46

GC content: 30.37

Gene sequence:

>540_bases
ATGAAGAATACTGCCTTACTCATTATCGATATGATTAATGATTTTCAATTTTCACACGGGCCCATCCTAGCCAAAAAATG
CGAAATGATAACCAATCCGATTTTACAATTAAAGAAAACAATGAAATCCTTCGGTTACCCCATCATTTATATTAATGACC
ATTATCAACTTTGGAGATCTGATATTGACCAATTAATTACTCATTGCACAAATGAATATAGTGAAAATATCATTCATAAG
ATTGCTCCAAGTTCTGATGATTACATTTTTATTAAACCACATTACTCTGCTTTTTATGAAACACCTTTAAATTCTTTATT
AGGCTATTTAAAAATAGAAAATCTAATATTAACAGGGGTGGCCGGGAATATATGTATCCTCTTTACAGCTAACGATGCTC
ATATGAGGAATTATACATTGTATGTACCCCAAGACTGTATCGCCTCTAATCATGATCAAGATAACCTACATGCTTTAAAA
ATAATGGAAACGACATTAAAAGCAAATATAATACCGTCATCTCAAATAAAATTTAATTAA

Upstream 100 bases:

>100_bases
TCCTTTCATTACAAATAAAGTACACTTATTTCCATTTACTACACATGGGATAACTTTTACAAATTCAATCCACCCTAAGA
AAAAAGAGAGGGGATCACAT

Downstream 100 bases:

>100_bases
TACATATATCTATTTTCTAACTTTTTATATCAAATTAACCCGCTCGCTTCCCTCTTGTGCTACAATGATTCTGTTTTGTG
TTTTTAGTAGTCACGGTAAA

Product: nicotinamidase

Products: 2,3-Dihydro-2,3-dihydroxybenzoate; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MKNTALLIIDMINDFQFSHGPILAKKCEMITNPILQLKKTMKSFGYPIIYINDHYQLWRSDIDQLITHCTNEYSENIIHK
IAPSSDDYIFIKPHYSAFYETPLNSLLGYLKIENLILTGVAGNICILFTANDAHMRNYTLYVPQDCIASNHDQDNLHALK
IMETTLKANIIPSSQIKFN

Sequences:

>Translated_179_residues
MKNTALLIIDMINDFQFSHGPILAKKCEMITNPILQLKKTMKSFGYPIIYINDHYQLWRSDIDQLITHCTNEYSENIIHK
IAPSSDDYIFIKPHYSAFYETPLNSLLGYLKIENLILTGVAGNICILFTANDAHMRNYTLYVPQDCIASNHDQDNLHALK
IMETTLKANIIPSSQIKFN
>Mature_179_residues
MKNTALLIIDMINDFQFSHGPILAKKCEMITNPILQLKKTMKSFGYPIIYINDHYQLWRSDIDQLITHCTNEYSENIIHK
IAPSSDDYIFIKPHYSAFYETPLNSLLGYLKIENLILTGVAGNICILFTANDAHMRNYTLYVPQDCIASNHDQDNLHALK
IMETTLKANIIPSSQIKFN

Specific function: Required For Production Of 2,3-Dhb. Also Serves As An Aryl Carrier Protein And Plays A Role In Enterobactin Assembly. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=190, Percent_Identity=26.8421052631579, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.3.2.1 [C]

Molecular weight: Translated: 20570; Mature: 20570

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTALLIIDMINDFQFSHGPILAKKCEMITNPILQLKKTMKSFGYPIIYINDHYQLWRS
CCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHH
DIDQLITHCTNEYSENIIHKIAPSSDDYIFIKPHYSAFYETPLNSLLGYLKIENLILTGV
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHEEEEEC
AGNICILFTANDAHMRNYTLYVPQDCIASNHDQDNLHALKIMETTLKANIIPSSQIKFN
CCCEEEEEECCCCCCCEEEEEECHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEECC
>Mature Secondary Structure
MKNTALLIIDMINDFQFSHGPILAKKCEMITNPILQLKKTMKSFGYPIIYINDHYQLWRS
CCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHH
DIDQLITHCTNEYSENIIHKIAPSSDDYIFIKPHYSAFYETPLNSLLGYLKIENLILTGV
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHEEEEEC
AGNICILFTANDAHMRNYTLYVPQDCIASNHDQDNLHALKIMETTLKANIIPSSQIKFN
CCCEEEEEECCCCCCCEEEEEECHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; Isochorismate [C]

Specific reaction: H2O + Isochorismate --> 2,3-Dihydro-2,3-dihydroxybenzoate + Pyruvate [C]

General reaction: Ether hydrolysis [C]

Inhibitor: Fe2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]