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The map label for this gene is rimO [H]

Identifier: 117922061

GI number: 117922061

Start: 4354424

End: 4355866

Strand: Direct

Name: rimO [H]

Synonym: Shewana3_3627

Alternate gene names: 117922061

Gene position: 4354424-4355866 (Clockwise)

Preceding gene: 117922059

Following gene: 117922063

Centisome position: 87.58

GC content: 51.56

Gene sequence:

>1443_bases
ATGACAGTTGAAACTTTTAATCCTAAGCAAACAACCACGCTTGAGACCCCAGCCAAGACATTAGAAGCAGCGAGTGCTGA
CTTAGCTAATGCCGAAAGTGCTACCGGGAATCGCATCGGTTTCGTGTCTTTGGGTTGCCCAAAAAACCTAGTGGACTCAG
AGCGCATTCTGACCCAATTACGCATCGACGGCTATGAAGTCACCAACAGCTACGATAATGCCGATCTGGTTATCGTCAAC
ACCTGTGGCTTTATCGATGCGGCGGTTGAAGAATCCTTAGATGCGGTGCGCGAAGCCCTAGAAGAAAACGGCAAAGTGAT
CGTGACTGGCTGTTTAGGCGCCAAGGAAAACCAAATTCGTGAAGTGCACCCAGACGTGCTCGAAATCACCGGCCCACACA
GCTACGAAGCCGTGTTAAAGCATGTACACAAGTACGTGCCAAAACCAGAGCACAACCCATTTACCTCGTTAATCCCACAA
ACTGGGGTTAAGTTAACGCCTAAGCATTACGCTTACTTAAAGATTTCAGAAGGTTGTGATAACCGTTGTACCTTCTGCAT
CATCCCAGCACTGCGTGGCGATTTAGATAGCCGTCCAGCCGGTAGCGTATTAGATGAAGCCAAGCGTTTGGTTGAATCAG
GCGTGCAAGAAATTTTAGTCGTAAGCCAAGACACCTCTGCCTACGGCAAAGACAAGGGCGGCCGCACCGATTTCTGGAAC
GGTATGCCGGTGAAGCAAGACATCACTAGCCTTGCCCGCCAACTGGGCAAGATGGGCGCTTGGGTACGTTTACACTACAT
CTACCCATACCCATGGGTTGACGATCTGATCCCATTAATGGCCGAAGGCTTAATCCTGCCTTACTTAGACATTCCAATGC
AGCACGCCAGCCCTCGCATCCTGAAAATGATGAAGCGTCCAGGCCGTGTTGACCGTCAGTTAGAAGCGATTCAACGCTGG
CGCGAAATCTGCCCAGACTTGGTGATCCGCTCAACCTTTATCGTGGGCTTCCCTGGCGAAACCGAAGAAGACTTCGAAAT
GTTGCTCGACTTCCTGCGTGAAGCGCGCCTCGACCGCGTAGGCTGCTTCAAGTACTCAGAAGTGGAAGGCGCAGTCGCCA
ACACCATCGCTGAACTTATCAGCGAAGACGTGAAGGAAGACAGATACCACAGATTTATGGAAGTCCAAGCCGAGATCAGC
GCCGAGCGTTTAGCCCGTTTCGTTGGCCGCACTATGGATATTCTGATCGATGACGTAGACGAAGAAGGCGCCATTGGCCG
CAGCTTCGCCGATGCCCCAGAAATCGACGGCATGGTCTTTATCAACGGTGAAACCGAACTCGAACCCGGCATGTTAGTCC
GCGCTGTGATCACTCACTCGGACGAGCACGATCTGTGGGCTGAGTTAGTCGACGCCGATGCAGAAGATGATATCGAGGCT
TAA

Upstream 100 bases:

>100_bases
GCTGTACACTTTTCACCCAAAGACACTGTACCTTTTAATCACTTCAGGTATAATCCGCGCTCTTTTTACGGCGCTCTTTT
ATCAGCATCAGCGAGACACC

Downstream 100 bases:

>100_bases
GCACTCGACTTAAGCGTGCTACTAAAGCGCTTGCCCTAAGCAGTCGATTAGCCGGATTAAAAACATAAATAGCGAGCCCT
TGATATTAATACTAATAGTC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MTVETFNPKQTTTLETPAKTLEAASADLANAESATGNRIGFVSLGCPKNLVDSERILTQLRIDGYEVTNSYDNADLVIVN
TCGFIDAAVEESLDAVREALEENGKVIVTGCLGAKENQIREVHPDVLEITGPHSYEAVLKHVHKYVPKPEHNPFTSLIPQ
TGVKLTPKHYAYLKISEGCDNRCTFCIIPALRGDLDSRPAGSVLDEAKRLVESGVQEILVVSQDTSAYGKDKGGRTDFWN
GMPVKQDITSLARQLGKMGAWVRLHYIYPYPWVDDLIPLMAEGLILPYLDIPMQHASPRILKMMKRPGRVDRQLEAIQRW
REICPDLVIRSTFIVGFPGETEEDFEMLLDFLREARLDRVGCFKYSEVEGAVANTIAELISEDVKEDRYHRFMEVQAEIS
AERLARFVGRTMDILIDDVDEEGAIGRSFADAPEIDGMVFINGETELEPGMLVRAVITHSDEHDLWAELVDADAEDDIEA

Sequences:

>Translated_480_residues
MTVETFNPKQTTTLETPAKTLEAASADLANAESATGNRIGFVSLGCPKNLVDSERILTQLRIDGYEVTNSYDNADLVIVN
TCGFIDAAVEESLDAVREALEENGKVIVTGCLGAKENQIREVHPDVLEITGPHSYEAVLKHVHKYVPKPEHNPFTSLIPQ
TGVKLTPKHYAYLKISEGCDNRCTFCIIPALRGDLDSRPAGSVLDEAKRLVESGVQEILVVSQDTSAYGKDKGGRTDFWN
GMPVKQDITSLARQLGKMGAWVRLHYIYPYPWVDDLIPLMAEGLILPYLDIPMQHASPRILKMMKRPGRVDRQLEAIQRW
REICPDLVIRSTFIVGFPGETEEDFEMLLDFLREARLDRVGCFKYSEVEGAVANTIAELISEDVKEDRYHRFMEVQAEIS
AERLARFVGRTMDILIDDVDEEGAIGRSFADAPEIDGMVFINGETELEPGMLVRAVITHSDEHDLWAELVDADAEDDIEA
>Mature_479_residues
TVETFNPKQTTTLETPAKTLEAASADLANAESATGNRIGFVSLGCPKNLVDSERILTQLRIDGYEVTNSYDNADLVIVNT
CGFIDAAVEESLDAVREALEENGKVIVTGCLGAKENQIREVHPDVLEITGPHSYEAVLKHVHKYVPKPEHNPFTSLIPQT
GVKLTPKHYAYLKISEGCDNRCTFCIIPALRGDLDSRPAGSVLDEAKRLVESGVQEILVVSQDTSAYGKDKGGRTDFWNG
MPVKQDITSLARQLGKMGAWVRLHYIYPYPWVDDLIPLMAEGLILPYLDIPMQHASPRILKMMKRPGRVDRQLEAIQRWR
EICPDLVIRSTFIVGFPGETEEDFEMLLDFLREARLDRVGCFKYSEVEGAVANTIAELISEDVKEDRYHRFMEVQAEISA
ERLARFVGRTMDILIDDVDEEGAIGRSFADAPEIDGMVFINGETELEPGMLVRAVITHSDEHDLWAELVDADAEDDIEA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=383, Percent_Identity=27.6762402088773, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI28872784, Length=456, Percent_Identity=25.8771929824561, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI28872782, Length=461, Percent_Identity=25.5965292841649, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1787057, Length=433, Percent_Identity=73.2101616628176, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=456, Percent_Identity=27.6315789473684, Blast_Score=142, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=479, Percent_Identity=25.4697286012526, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71996771, Length=216, Percent_Identity=31.0185185185185, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21356207, Length=421, Percent_Identity=25.1781472684086, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI19922432, Length=414, Percent_Identity=25.6038647342995, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53567; Mature: 53436

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVETFNPKQTTTLETPAKTLEAASADLANAESATGNRIGFVSLGCPKNLVDSERILTQL
CCCCCCCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
RIDGYEVTNSYDNADLVIVNTCGFIDAAVEESLDAVREALEENGKVIVTGCLGAKENQIR
HCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHH
EVHPDVLEITGPHSYEAVLKHVHKYVPKPEHNPFTSLIPQTGVKLTPKHYAYLKISEGCD
HCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEECCCCEEEEEECCCCC
NRCTFCIIPALRGDLDSRPAGSVLDEAKRLVESGVQEILVVSQDTSAYGKDKGGRTDFWN
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCC
GMPVKQDITSLARQLGKMGAWVRLHYIYPYPWVDDLIPLMAEGLILPYLDIPMQHASPRI
CCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCEECHHCCCCCCCCHHH
LKMMKRPGRVDRQLEAIQRWREICPDLVIRSTFIVGFPGETEEDFEMLLDFLREARLDRV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHH
GCFKYSEVEGAVANTIAELISEDVKEDRYHRFMEVQAEISAERLARFVGRTMDILIDDVD
CCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCC
EEGAIGRSFADAPEIDGMVFINGETELEPGMLVRAVITHSDEHDLWAELVDADAEDDIEA
CCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEHHHHHCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TVETFNPKQTTTLETPAKTLEAASADLANAESATGNRIGFVSLGCPKNLVDSERILTQL
CCCCCCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
RIDGYEVTNSYDNADLVIVNTCGFIDAAVEESLDAVREALEENGKVIVTGCLGAKENQIR
HCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHH
EVHPDVLEITGPHSYEAVLKHVHKYVPKPEHNPFTSLIPQTGVKLTPKHYAYLKISEGCD
HCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEECCCCEEEEEECCCCC
NRCTFCIIPALRGDLDSRPAGSVLDEAKRLVESGVQEILVVSQDTSAYGKDKGGRTDFWN
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCC
GMPVKQDITSLARQLGKMGAWVRLHYIYPYPWVDDLIPLMAEGLILPYLDIPMQHASPRI
CCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCEECHHCCCCCCCCHHH
LKMMKRPGRVDRQLEAIQRWREICPDLVIRSTFIVGFPGETEEDFEMLLDFLREARLDRV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHH
GCFKYSEVEGAVANTIAELISEDVKEDRYHRFMEVQAEISAERLARFVGRTMDILIDDVD
CCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCC
EEGAIGRSFADAPEIDGMVFINGETELEPGMLVRAVITHSDEHDLWAELVDADAEDDIEA
CCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEHHHHHCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]