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The map label for this gene is leuO [H]

Identifier: 117921998

GI number: 117921998

Start: 4270905

End: 4271750

Strand: Direct

Name: leuO [H]

Synonym: Shewana3_3562

Alternate gene names: 117921998

Gene position: 4270905-4271750 (Clockwise)

Preceding gene: 117921988

Following gene: 117921999

Centisome position: 85.9

GC content: 41.96

Gene sequence:

>846_bases
ATGGATTTAAATTTAGTACGTACATTTTTAGTGGTGGCAGAGTGCCAGTCCTATACCAAGGCCGCCGAACGTATGCAATT
GACCCAGCCCGCGATTAGCGCCGCCATTAAGCGATTAGAAAAACTCTACGGTGAAAATCTGTTTATCAAGCAAGGGCGAG
GCATAGAACTCTCCAGCAAAGGGCAGCAACTTATCCCCTCCTTTCGTCAAGCTGTCAGCATTATCGAAAATGCGATGAAT
ATGCGAAAGCAGTTTAATGTTTACTGTAATGAGTCGCTGATAAATACCTTATTCCCCATTGATAACATTAACTTGCAAGA
ATCTCCGCCGGAGAAAAACCAGCTGTTTGAACACCTGCGCCAAGAGAAAACCGACATTATTGTCGATAGTATGTTGACTC
GAGACAGTGCATTTATGGTGGAGGAAATTCTCTTAGAGCCCTTAGTGGTGATTTGCCGAAAAGACCATCCACGAATTCAG
CAAAGCATCACCAAAGACGCCTTTTATCGTGAAGATCATGTGTTTTATAGCGGCACGTGGGAAAAGATCCGTGGCTTCGA
ACTCTTTGCCAACGAACCCATTGAAGAACGCAAAATGGCATTAATCTGTCATTCGATTGCCAGCATTGCCTTGAATATTT
CTCAGAGTGATTCAATTGCCGTTATCGCTCGCTCCTTTGCCGAGCAATGGGCAGCAAAATTAAATCTGCAAATATTAACT
TGCCCCATTAATACCGATTTAATTCCCTATCATTTGGTGTATCACAAACGACAAATTAAAAATCCCCTGCATAAAAAAAT
CAGAGAAGAAATAAAAGCCAAGATAAAGGCTTTACATAGGCAATAA

Upstream 100 bases:

>100_bases
ACAAAATATAAACATCCCATCCAGATTAGGCGCAAAACGCACAAGAAACAATCAACAAAATGTTTTACGATACATAAATC
AAATTTATGAATTGAAGATT

Downstream 100 bases:

>100_bases
TTGCTAATTCGAGCTTCAACAATGGGTGTCAATGTAACCATTGCACCCGTAAAACACTTCCCAGAAATACAGTCGCAGTA
TCACAGTCGCAGTATCACAG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MDLNLVRTFLVVAECQSYTKAAERMQLTQPAISAAIKRLEKLYGENLFIKQGRGIELSSKGQQLIPSFRQAVSIIENAMN
MRKQFNVYCNESLINTLFPIDNINLQESPPEKNQLFEHLRQEKTDIIVDSMLTRDSAFMVEEILLEPLVVICRKDHPRIQ
QSITKDAFYREDHVFYSGTWEKIRGFELFANEPIEERKMALICHSIASIALNISQSDSIAVIARSFAEQWAAKLNLQILT
CPINTDLIPYHLVYHKRQIKNPLHKKIREEIKAKIKALHRQ

Sequences:

>Translated_281_residues
MDLNLVRTFLVVAECQSYTKAAERMQLTQPAISAAIKRLEKLYGENLFIKQGRGIELSSKGQQLIPSFRQAVSIIENAMN
MRKQFNVYCNESLINTLFPIDNINLQESPPEKNQLFEHLRQEKTDIIVDSMLTRDSAFMVEEILLEPLVVICRKDHPRIQ
QSITKDAFYREDHVFYSGTWEKIRGFELFANEPIEERKMALICHSIASIALNISQSDSIAVIARSFAEQWAAKLNLQILT
CPINTDLIPYHLVYHKRQIKNPLHKKIREEIKAKIKALHRQ
>Mature_281_residues
MDLNLVRTFLVVAECQSYTKAAERMQLTQPAISAAIKRLEKLYGENLFIKQGRGIELSSKGQQLIPSFRQAVSIIENAMN
MRKQFNVYCNESLINTLFPIDNINLQESPPEKNQLFEHLRQEKTDIIVDSMLTRDSAFMVEEILLEPLVVICRKDHPRIQ
QSITKDAFYREDHVFYSGTWEKIRGFELFANEPIEERKMALICHSIASIALNISQSDSIAVIARSFAEQWAAKLNLQILT
CPINTDLIPYHLVYHKRQIKNPLHKKIREEIKAKIKALHRQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=290, Percent_Identity=24.8275862068966, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI87081766, Length=288, Percent_Identity=21.5277777777778, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI145693105, Length=208, Percent_Identity=21.1538461538462, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786820, Length=285, Percent_Identity=22.8070175438596, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32535; Mature: 32535

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLNLVRTFLVVAECQSYTKAAERMQLTQPAISAAIKRLEKLYGENLFIKQGRGIELSSK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
GQQLIPSFRQAVSIIENAMNMRKQFNVYCNESLINTLFPIDNINLQESPPEKNQLFEHLR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
QEKTDIIVDSMLTRDSAFMVEEILLEPLVVICRKDHPRIQQSITKDAFYREDHVFYSGTW
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCCH
EKIRGFELFANEPIEERKMALICHSIASIALNISQSDSIAVIARSFAEQWAAKLNLQILT
HHHCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEE
CPINTDLIPYHLVYHKRQIKNPLHKKIREEIKAKIKALHRQ
ECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLNLVRTFLVVAECQSYTKAAERMQLTQPAISAAIKRLEKLYGENLFIKQGRGIELSSK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
GQQLIPSFRQAVSIIENAMNMRKQFNVYCNESLINTLFPIDNINLQESPPEKNQLFEHLR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
QEKTDIIVDSMLTRDSAFMVEEILLEPLVVICRKDHPRIQQSITKDAFYREDHVFYSGTW
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCCH
EKIRGFELFANEPIEERKMALICHSIASIALNISQSDSIAVIARSFAEQWAAKLNLQILT
HHHCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEE
CPINTDLIPYHLVYHKRQIKNPLHKKIREEIKAKIKALHRQ
ECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]