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The map label for this gene is napA [H]

Identifier: 117921867

GI number: 117921867

Start: 4105840

End: 4108320

Strand: Direct

Name: napA [H]

Synonym: Shewana3_3430

Alternate gene names: 117921867

Gene position: 4105840-4108320 (Clockwise)

Preceding gene: 117921866

Following gene: 117921868

Centisome position: 82.58

GC content: 51.63

Gene sequence:

>2481_bases
ATGAGCATTAGCCGCCGCGAGTTTCTCAAGGCTAACGCAGCGGTTGCCGCTGCCACGGCCGTTGGCGTCACGCTTCCCGT
GAAAATGGTCGAAGCCGCCGAGTCAGATAACATTAAGTGGGATAAAGCCCCCTGCCGTTTTTGCGGTGTGGGTTGTAGCG
TATTAGTCGGTACCAAAGCCGGTAAAGTCGTCGCGACTAAGGGCGATCCCGAAAGCCCAGTTAACCGTGGTTTGAACTGT
ATTAAGGGTTACTTCCTGTCGAAAATCATGTACGGCAAGGACAGATTAACCACGCCGCTGCTGCGGATGAAAGACGGCAA
ATACCACAAAGAAGGCGAGTTTACCCCAGTAAGCTGGGATGTTGCCTTCGATACTATGGCCGCTAAGTGGAAAAACAGCA
TCGCCACTAAGGGCCCTACCTCAGTCGGTATGTTCGGCTCGGGCCAGTGGACCATTTGGGAAGGTTATGCCGCCTCTAAA
TTACATAAGGCCGGATTCTTAACCAACAATATCGACCCTAACGCCCGCCACTGCATGGCATCGGCCGTAGGTGGCTTTAT
GCGTACCTTCGGTATCGACGAGCCTATGGGGTGTTACGATGACTTAGAAGCCGCCGACCAATTTGTGCTCTGGGGCGCTA
ACATGGCAGAGATGCACCCGATCCTGTGGGCACGCTTATCCGACCGTCGTTTAAGCCACAAAGACTGCCGCGTACATGTG
CTATCCACCTTCGAGAACCGCAGTTTCGACTTAGCGGACAATCCTATGGTGTTCCGTCCACAGTCAGACTTAGTGATCCT
CAACTTTATCGCTAACTACATCATCCAACACAAAGCCGTGAATACCGACTTTGTGACTAAACATACTAAGTTTGCTCTCG
GTGTGGATGATATCGGTTATGGTCTACGTCCAGATCATCCGTTAGAGAAGAAAGCCAAAAACCCAGGCAATGGTAAGTCT
AGCCCTATCAGCTTCGAAGAATACGCTAAGTTCGTCAGCACTTATACGCTGGAATATGCGGCGAAAATGAGTGGTGTAGA
GCCTGAAAAATTAGAAACCTTAGCTAAGGCCTACGCCGATCCTAAGGTCAAAGTCATGAGTCTGTGGACCATGGGGATCA
ACCAGCACGTACGTGGTGTGTGGGCAAACAACATGCTCTACAACATCCACTTACTCACAGGTAAAATCGCTACCCCAGGT
AACAGCCCATTCTCATTAACGGGTCAACCTTCGGCCTGTGGTACTGCCCGTGAAGTCGGCACCTTCGCCCACCGCTTACC
TGCGGATATGGAAGTGGCCAACGATAAACACCGCGCCATCACAGAAAAAGTGTGGCAGCTACCAGAAGGCACCATTCCGC
CAAAACCCGGCTTCCATGCCGTGCTGCAAAGCCGCATGCTCAAAGACGGTAAGCTCAACTGCTATTGGACCATGTGTACC
AACAATATGCAGGCGGGTCCGAATATCAACGATGAAATCTACCCCGGTTTCCGTAACCCAGAAAACTTTATCGTGGTATC
CGATCCATACCCAACCGTCACCGCCATGGCCGCTGACTTAATTCTGCCCACCGCCATGTGGGTTGAGAAAGAAGGTGCCT
ACGGTAACGCCGAGCGCCGCACCCATATGTGGCATCAACAGGTCAAAGCGCCAGAAGGGGCCAAGTCGGATCTGTGGCAA
TTAGTCGAGTTCTCAAAACGCTTTAAAGTGTCTGAAGTGTGGCCGGCTGAGCTTATCGCTAAACAGCCAGAATATGCTGA
CAAAACGCTTTATGAAGTGCTGTTTGCCAACGGCGTCATCAACAAGTTCCCAACCACAGATTGTAAGGGCGACTTAAACG
ATGAAAGTGCGCACTTTGGCTTCTATCTGCAAAAAGGCATTTTCGAAGAATATGCCGCCTTTGGTCGCGGCCATGGCCAT
GACTTAGCCGACTTTGACCGTTACCACGAAACCCGTGGCCTACGCTGGCCTGTGGTTGAGGGCAAAGAAACCCTGCGCCG
CTTCGTTGAAGGTAGCGATCCTTATGTGAAGGCAGGAGAAGGCTACAAGTTCTACGGTAAACCCGATGGCAAAGCGGTGA
TTTTCGCCCTGCCCTACGAACCTGCAGCAGAAGAGCCAAATGAAGAATACGATCTGTGGATGTCTACCGGCCGTGTACTC
GAACACTGGCATACAGGTTCGATGACCGCCCGTGTACCTGAGCTGTACCGCGCTTACCCCGATGCACAAATCTTTATGCA
TCCTGAAGACGCTAAAGCCCGTGGTCTGCAACGTGGTGATGAAGTGCTAGTCGCATCCCCCCGTGGTGAGGTCAAAACCC
GTGTCGAAACCAAAGGCCGTAACAAACCGCCACGCGGCGTGGTGTTTATGCCCTTCTTCGATGCGCGTCAGTTAGTTAAC
AAGTTGATTTTGGATGCGACCGATCCACTGTCAAAAGAAACTGACTTTAAGAAGTGTCCCGTAAAAGTGATGAAGGCCTA
A

Upstream 100 bases:

>100_bases
TCGACAATGTTGAAGCCATCAATGCCCTGTCCGGCGTGTTATCCGCCAGTTTGATTTACCACCAAGTAGAACCCTTAGAA
CAAGAGAGTGAGGAAACACT

Downstream 100 bases:

>100_bases
GCCTCATTTGATAGCAATAAGCGTATTTGAAAGCGATTAAGGAGTGCTAAGGTGAGTCAGCAAGTGAAGAGTCCATTCAC
AGTCAAGCAAGTCAACCGTC

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 826; Mature: 825

Protein sequence:

>826_residues
MSISRREFLKANAAVAAATAVGVTLPVKMVEAAESDNIKWDKAPCRFCGVGCSVLVGTKAGKVVATKGDPESPVNRGLNC
IKGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPVSWDVAFDTMAAKWKNSIATKGPTSVGMFGSGQWTIWEGYAASK
LHKAGFLTNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSHKDCRVHV
LSTFENRSFDLADNPMVFRPQSDLVILNFIANYIIQHKAVNTDFVTKHTKFALGVDDIGYGLRPDHPLEKKAKNPGNGKS
SPISFEEYAKFVSTYTLEYAAKMSGVEPEKLETLAKAYADPKVKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATPG
NSPFSLTGQPSACGTAREVGTFAHRLPADMEVANDKHRAITEKVWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMCT
NNMQAGPNINDEIYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGAKSDLWQ
LVEFSKRFKVSEVWPAELIAKQPEYADKTLYEVLFANGVINKFPTTDCKGDLNDESAHFGFYLQKGIFEEYAAFGRGHGH
DLADFDRYHETRGLRWPVVEGKETLRRFVEGSDPYVKAGEGYKFYGKPDGKAVIFALPYEPAAEEPNEEYDLWMSTGRVL
EHWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLQRGDEVLVASPRGEVKTRVETKGRNKPPRGVVFMPFFDARQLVN
KLILDATDPLSKETDFKKCPVKVMKA

Sequences:

>Translated_826_residues
MSISRREFLKANAAVAAATAVGVTLPVKMVEAAESDNIKWDKAPCRFCGVGCSVLVGTKAGKVVATKGDPESPVNRGLNC
IKGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPVSWDVAFDTMAAKWKNSIATKGPTSVGMFGSGQWTIWEGYAASK
LHKAGFLTNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSHKDCRVHV
LSTFENRSFDLADNPMVFRPQSDLVILNFIANYIIQHKAVNTDFVTKHTKFALGVDDIGYGLRPDHPLEKKAKNPGNGKS
SPISFEEYAKFVSTYTLEYAAKMSGVEPEKLETLAKAYADPKVKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATPG
NSPFSLTGQPSACGTAREVGTFAHRLPADMEVANDKHRAITEKVWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMCT
NNMQAGPNINDEIYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGAKSDLWQ
LVEFSKRFKVSEVWPAELIAKQPEYADKTLYEVLFANGVINKFPTTDCKGDLNDESAHFGFYLQKGIFEEYAAFGRGHGH
DLADFDRYHETRGLRWPVVEGKETLRRFVEGSDPYVKAGEGYKFYGKPDGKAVIFALPYEPAAEEPNEEYDLWMSTGRVL
EHWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLQRGDEVLVASPRGEVKTRVETKGRNKPPRGVVFMPFFDARQLVN
KLILDATDPLSKETDFKKCPVKVMKA
>Mature_825_residues
SISRREFLKANAAVAAATAVGVTLPVKMVEAAESDNIKWDKAPCRFCGVGCSVLVGTKAGKVVATKGDPESPVNRGLNCI
KGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPVSWDVAFDTMAAKWKNSIATKGPTSVGMFGSGQWTIWEGYAASKL
HKAGFLTNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSHKDCRVHVL
STFENRSFDLADNPMVFRPQSDLVILNFIANYIIQHKAVNTDFVTKHTKFALGVDDIGYGLRPDHPLEKKAKNPGNGKSS
PISFEEYAKFVSTYTLEYAAKMSGVEPEKLETLAKAYADPKVKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATPGN
SPFSLTGQPSACGTAREVGTFAHRLPADMEVANDKHRAITEKVWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMCTN
NMQAGPNINDEIYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGAKSDLWQL
VEFSKRFKVSEVWPAELIAKQPEYADKTLYEVLFANGVINKFPTTDCKGDLNDESAHFGFYLQKGIFEEYAAFGRGHGHD
LADFDRYHETRGLRWPVVEGKETLRRFVEGSDPYVKAGEGYKFYGKPDGKAVIFALPYEPAAEEPNEEYDLWMSTGRVLE
HWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLQRGDEVLVASPRGEVKTRVETKGRNKPPRGVVFMPFFDARQLVNK
LILDATDPLSKETDFKKCPVKVMKA

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=827, Percent_Identity=72.6723095525998, Blast_Score=1271, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=793, Percent_Identity=23.7074401008827, Blast_Score=191, Evalue=2e-49,
Organism=Escherichia coli, GI3868720, Length=604, Percent_Identity=24.5033112582781, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI3868719, Length=489, Percent_Identity=24.5398773006135, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI87081797, Length=832, Percent_Identity=21.2740384615385, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787870, Length=557, Percent_Identity=22.0825852782765, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 92352; Mature: 92221

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISRREFLKANAAVAAATAVGVTLPVKMVEAAESDNIKWDKAPCRFCGVGCSVLVGTKA
CCCCHHHHHHHHHHHHHHHHHCEECHHHHHHHCCCCCCEECCCCCHHHCCCCEEEEECCC
GKVVATKGDPESPVNRGLNCIKGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPVSWD
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCCCCCCCCCCCCCEEEEH
VAFDTMAAKWKNSIATKGPTSVGMFGSGQWTIWEGYAASKLHKAGFLTNNIDPNARHCMA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEECCCCCCHHHHHH
SAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSHKDCRVHV
HHHHHHHHHCCCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEE
LSTFENRSFDLADNPMVFRPQSDLVILNFIANYIIQHKAVNTDFVTKHTKFALGVDDIGY
EEECCCCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHHCCCCCHHCCCCEEEECHHHCCC
GLRPDHPLEKKAKNPGNGKSSPISFEEYAKFVSTYTLEYAAKMSGVEPEKLETLAKAYAD
CCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
PKVKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATPGNSPFSLTGQPSACGTAREVG
CCEEEEEEEECCCCHHHCEEEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCHHHHH
TFAHRLPADMEVANDKHRAITEKVWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMCT
HHHHHCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEC
NNMQAGPNINDEIYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAERR
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHHH
THMWHQQVKAPEGAKSDLWQLVEFSKRFKVSEVWPAELIAKQPEYADKTLYEVLFANGVI
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCC
NKFPTTDCKGDLNDESAHFGFYLQKGIFEEYAAFGRGHGHDLADFDRYHETRGLRWPVVE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
GKETLRRFVEGSDPYVKAGEGYKFYGKPDGKAVIFALPYEPAAEEPNEEYDLWMSTGRVL
CHHHHHHHHCCCCCEEECCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
EHWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLQRGDEVLVASPRGEVKTRVETKGR
HHHCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHCCCCCCEEEEECCCCCCEEEHHHCCC
NKPPRGVVFMPFFDARQLVNKLILDATDPLSKETDFKKCPVKVMKA
CCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
SISRREFLKANAAVAAATAVGVTLPVKMVEAAESDNIKWDKAPCRFCGVGCSVLVGTKA
CCCHHHHHHHHHHHHHHHHHCEECHHHHHHHCCCCCCEECCCCCHHHCCCCEEEEECCC
GKVVATKGDPESPVNRGLNCIKGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPVSWD
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCCCCCCCCCCCCCEEEEH
VAFDTMAAKWKNSIATKGPTSVGMFGSGQWTIWEGYAASKLHKAGFLTNNIDPNARHCMA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEECCCCCCHHHHHH
SAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSHKDCRVHV
HHHHHHHHHCCCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEE
LSTFENRSFDLADNPMVFRPQSDLVILNFIANYIIQHKAVNTDFVTKHTKFALGVDDIGY
EEECCCCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHHCCCCCHHCCCCEEEECHHHCCC
GLRPDHPLEKKAKNPGNGKSSPISFEEYAKFVSTYTLEYAAKMSGVEPEKLETLAKAYAD
CCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
PKVKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATPGNSPFSLTGQPSACGTAREVG
CCEEEEEEEECCCCHHHCEEEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCHHHHH
TFAHRLPADMEVANDKHRAITEKVWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMCT
HHHHHCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEC
NNMQAGPNINDEIYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAERR
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHHH
THMWHQQVKAPEGAKSDLWQLVEFSKRFKVSEVWPAELIAKQPEYADKTLYEVLFANGVI
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCC
NKFPTTDCKGDLNDESAHFGFYLQKGIFEEYAAFGRGHGHDLADFDRYHETRGLRWPVVE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
GKETLRRFVEGSDPYVKAGEGYKFYGKPDGKAVIFALPYEPAAEEPNEEYDLWMSTGRVL
CHHHHHHHHCCCCCEEECCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
EHWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLQRGDEVLVASPRGEVKTRVETKGR
HHHCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHCCCCCCEEEEECCCCCCEEEHHHCCC
NKPPRGVVFMPFFDARQLVNKLILDATDPLSKETDFKKCPVKVMKA
CCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]