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The map label for this gene is rumB

Identifier: 117921747

GI number: 117921747

Start: 3971082

End: 3972236

Strand: Reverse

Name: rumB

Synonym: Shewana3_3309

Alternate gene names: 117921747

Gene position: 3972236-3971082 (Counterclockwise)

Preceding gene: 117921748

Following gene: 117921742

Centisome position: 79.89

GC content: 50.82

Gene sequence:

>1155_bases
GTGAGTACATTAGTACAATGTGGGTATTTTGAACGAGGCCAATGCCAATCCTGTCGCCATATTAAGCTCCCTATGGCGCA
GCAACTGGCGGCCAAAAACCTAGAATTGCAGCAATTGTTAGCGCCTTTTGTCGATAAGACCGAACCGCAATTTTTACCAC
CGGTTGTGGGTGACAGCAGCGGTTTTCGTAACAAGGCGAAAATGGTCGCCTTGGGCGCAGCCCATGCGCCTGTGTTGGGG
ATTGTTAGTCCCAGCGGCGAAGCGGTGAGCCTGTGTGACTGCCTGTTGTATCCTACTGATATGCAAGCCTTACTCCACCG
TTTAGAGCGCTTTGTACAGCAAGCGGGCATTCCGCCTTACCGTGTCGATAAGGCCAAGGGCGAGCTTAAATTTATCCTAC
TGACGCGCAGCCAAGTGCGTGGCGAGTATATGTTGCGGTTTGTGCTGCGCTCGCGGGATGCCATCGCCCGTATCGAGCGT
GAATTGCCTACGCTGATGGCCGAATATCCGCAGATCAAAGTCGTATCCGTCAATTTGCAACCTGTTCATATGGCTATCTT
GGAGGGTGAGGAGGAGATTTTCCTCACTGAAAATACTCGCCTCGAAGAGCGCTTTAACGATGTGCCTTTGTTTATTCGTC
CCAAGAGCTTTTTTCAAACCAATCCGCAGGTGGCGGCAAAGCTGTACCAAACCGCCCGTGAGTGGGTGGCCGACTTCGCG
CCAGCTTCCCTTTGGGACTTATTCTGTGGTGTGGGCGGCTTTGGTTTGCACTGTGCAGCTAAAGATATCCCACTGACAGG
GATTGAAATTGAAGCCGAAGCCATTGCCTGCGCTAAGATGTCGGCGCAGTTGATGGGATTGGATAAAGTAGAATTTATGG
CGCTCGATTCCACCGACTTTGCCAAGGGCGAGGCGGCGCAAACTAAGCCTGAGTTGATTATTGTCAATCCACCGCGCCGC
GGGATTGGTGAGTCCCTGTGCCATTCCTTAAGTGAGTTTGCGCCTAAGGCGATTTTGTATTCCAGCTGCAATCCTAAAAC
CTTAGCCAAAGATCTCGGCCATATCCGTGGCTATCGGTTAACTAAGGTGCAGTTATTCGATCTTTTTCCTCACTCTGATC
ATTTCGAAGTGTTAGCCTTATTAGTCAAAGACTAA

Upstream 100 bases:

>100_bases
ACTGGGGCCTTAATGTGTTTGTTATGCTTCAGTTGTCATCCAAATTTCACTCGCCATCTCAATAGATAAGATAGACGGGC
ACTGCTGTGGGTACGATGCT

Downstream 100 bases:

>100_bases
ACCTCAGCACTCAATTACTCGGCGTCAGTTTCGGCTTTAGGCGTTAAGGCATCGTCCCAGCTAATGCTGATTTCGGCGCC
GCCTAGGGCCTGTGAGCGGC

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSTLVQCGYFERGQCQSCRHIKLPMAQQLAAKNLELQQLLAPFVDKTEPQFLPPVVGDSSGFRNKAKMVALGAAHAPVLG
IVSPSGEAVSLCDCLLYPTDMQALLHRLERFVQQAGIPPYRVDKAKGELKFILLTRSQVRGEYMLRFVLRSRDAIARIER
ELPTLMAEYPQIKVVSVNLQPVHMAILEGEEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPQVAAKLYQTAREWVADFA
PASLWDLFCGVGGFGLHCAAKDIPLTGIEIEAEAIACAKMSAQLMGLDKVEFMALDSTDFAKGEAAQTKPELIIVNPPRR
GIGESLCHSLSEFAPKAILYSSCNPKTLAKDLGHIRGYRLTKVQLFDLFPHSDHFEVLALLVKD

Sequences:

>Translated_384_residues
MSTLVQCGYFERGQCQSCRHIKLPMAQQLAAKNLELQQLLAPFVDKTEPQFLPPVVGDSSGFRNKAKMVALGAAHAPVLG
IVSPSGEAVSLCDCLLYPTDMQALLHRLERFVQQAGIPPYRVDKAKGELKFILLTRSQVRGEYMLRFVLRSRDAIARIER
ELPTLMAEYPQIKVVSVNLQPVHMAILEGEEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPQVAAKLYQTAREWVADFA
PASLWDLFCGVGGFGLHCAAKDIPLTGIEIEAEAIACAKMSAQLMGLDKVEFMALDSTDFAKGEAAQTKPELIIVNPPRR
GIGESLCHSLSEFAPKAILYSSCNPKTLAKDLGHIRGYRLTKVQLFDLFPHSDHFEVLALLVKD
>Mature_383_residues
STLVQCGYFERGQCQSCRHIKLPMAQQLAAKNLELQQLLAPFVDKTEPQFLPPVVGDSSGFRNKAKMVALGAAHAPVLGI
VSPSGEAVSLCDCLLYPTDMQALLHRLERFVQQAGIPPYRVDKAKGELKFILLTRSQVRGEYMLRFVLRSRDAIARIERE
LPTLMAEYPQIKVVSVNLQPVHMAILEGEEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPQVAAKLYQTAREWVADFAP
ASLWDLFCGVGGFGLHCAAKDIPLTGIEIEAEAIACAKMSAQLMGLDKVEFMALDSTDFAKGEAAQTKPELIIVNPPRRG
IGESLCHSLSEFAPKAILYSSCNPKTLAKDLGHIRGYRLTKVQLFDLFPHSDHFEVLALLVKD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily

Homologues:

Organism=Homo sapiens, GI34222389, Length=183, Percent_Identity=28.9617486338798, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI51173878, Length=183, Percent_Identity=28.9617486338798, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1787083, Length=379, Percent_Identity=54.6174142480211, Blast_Score=430, Evalue=1e-122,
Organism=Escherichia coli, GI1789148, Length=182, Percent_Identity=30.7692307692308, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790403, Length=185, Percent_Identity=28.1081081081081, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24666579, Length=422, Percent_Identity=24.6445497630332, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMB_SHESA (A0L0G4)

Other databases:

- EMBL:   CP000469
- RefSeq:   YP_870939.1
- ProteinModelPortal:   A0L0G4
- SMR:   A0L0G4
- STRING:   A0L0G4
- GeneID:   4477610
- GenomeReviews:   CP000469_GR
- KEGG:   shn:Shewana3_3309
- NMPDR:   fig|94122.5.peg.3320
- eggNOG:   COG2265
- HOGENOM:   HBG296593
- OMA:   QPQHAAI
- PhylomeDB:   A0L0G4
- ProtClustDB:   PRK03522
- BioCyc:   SSP94122:SHEWANA3_3309-MONOMER
- HAMAP:   MF_01012
- InterPro:   IPR011825
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02085

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.189

Molecular weight: Translated: 42758; Mature: 42627

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 343-343 BINDING 219-219 BINDING 248-248 BINDING 269-269 BINDING 316-316

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLVQCGYFERGQCQSCRHIKLPMAQQLAAKNLELQQLLAPFVDKTEPQFLPPVVGDSS
CCCCEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
GFRNKAKMVALGAAHAPVLGIVSPSGEAVSLCDCLLYPTDMQALLHRLERFVQQAGIPPY
CCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCE
RVDKAKGELKFILLTRSQVRGEYMLRFVLRSRDAIARIERELPTLMAEYPQIKVVSVNLQ
EECCCCCCEEEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECC
PVHMAILEGEEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPQVAAKLYQTAREWVADFA
EEEEEEECCCCEEEEECCCCHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCC
PASLWDLFCGVGGFGLHCAAKDIPLTGIEIEAEAIACAKMSAQLMGLDKVEFMALDSTDF
CHHHHHHHHCCCCCCEEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCC
AKGEAAQTKPELIIVNPPRRGIGESLCHSLSEFAPKAILYSSCNPKTLAKDLGHIRGYRL
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCEE
TKVQLFDLFPHSDHFEVLALLVKD
EEEEEEECCCCCCCHHHHHEEECC
>Mature Secondary Structure 
STLVQCGYFERGQCQSCRHIKLPMAQQLAAKNLELQQLLAPFVDKTEPQFLPPVVGDSS
CCCEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
GFRNKAKMVALGAAHAPVLGIVSPSGEAVSLCDCLLYPTDMQALLHRLERFVQQAGIPPY
CCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCE
RVDKAKGELKFILLTRSQVRGEYMLRFVLRSRDAIARIERELPTLMAEYPQIKVVSVNLQ
EECCCCCCEEEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECC
PVHMAILEGEEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPQVAAKLYQTAREWVADFA
EEEEEEECCCCEEEEECCCCHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCC
PASLWDLFCGVGGFGLHCAAKDIPLTGIEIEAEAIACAKMSAQLMGLDKVEFMALDSTDF
CHHHHHHHHCCCCCCEEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCC
AKGEAAQTKPELIIVNPPRRGIGESLCHSLSEFAPKAILYSSCNPKTLAKDLGHIRGYRL
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCEE
TKVQLFDLFPHSDHFEVLALLVKD
EEEEEEECCCCCCCHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA