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The map label for this gene is rstB [H]

Identifier: 117921746

GI number: 117921746

Start: 3969838

End: 3971067

Strand: Direct

Name: rstB [H]

Synonym: Shewana3_3308

Alternate gene names: 117921746

Gene position: 3969838-3971067 (Clockwise)

Preceding gene: 117921745

Following gene: 117921750

Centisome position: 79.84

GC content: 48.13

Gene sequence:

>1230_bases
ATGAAATTTCAGTTCTACCGCTTGTTTATCTTTTTACTGCTGTCCTGCGGAATCGTGATCTGGAGCTTTGGTCAGCTCGC
CGAGCATTTCGCCCACGACGAATACAACTACCAAATCGACGTCGACGACCTACTGCAAAATTATGATGAGCAACTACCTA
AGATAAAGCGCATTCCCGTTGCCTCTCTCTCGCTGCCAGAAAATTTGCAATCCTCACTGCAACTGGGGGCGACAATCGCC
CTGCGCCATAGCAGTAATGAGCTCTATTATTATCGCCTCGATAAGGATAGCAGCCATATCTTAGTGCTAGGCCCAGTGCA
AACACCTGTGCCGAAGGAGCGCAATACCAGTTTGATTTTATTGGGATTATACTCATCACTCTGCTTGGTTGCCCTCTGGT
GGATTTGGCCGGTATTTCGGGATCTTAACCATTTGCAGCAGGCCGCTATCGAATTTGGCCAAGCCCCGCGTAAACGACCT
CAGGATATCCATAAACACTCGGCAATTTTCCCGCTTGCTAAGGCTTTTAACAGTGTTAGCCACCAAATCGTCGACTTTAT
CCAGATGCACAAGGAGCTGTCCCGCACCATCTCCCACGAGGTACGCACGCCTCTTGCGCGAATGCGCTTTGCACTGGAAA
TTATTCGGCCACAAATCGATGCTAATTACGCTAACCGCTTAAATGAAGATATCGATGATATTGAACAACTTGCAGCAAAT
TATCTGTCTTTTGCTCGCCTAGAGCATAAGGAAGAAACCCTCAGCCAAGAATCACAATCCATTGAATTATTTATGGAAAA
ATTGGCCCACAAATATGCTATCTACCAGCCTAAGTTTAATATTGTGTTTCACTATGAGGCGCAGCAGGCCCACTTCGACC
CGATTGCGATGACGATTGCGATTCAAAACCTTGTCCAAAATGCTATGCGTTTCGCCCAGCATGACATCCATGTCCACTTC
CATCAGCAACGAGGCATAAATCGGATCAGCGTCGAGGATGATGGTCCGGGATTTGAAGGCAAAGGCAAAAAACTGGTAGC
AGCCTTCGAGCGGGATAGCCAGCAGAGCGATACTAGTGGCTATGGTTTAGGGCTGTATATCGTCAAGAAAATCGCCACTT
GGCATTATGGCACGCTAGAGTTAAGCCGCTCACAGGCCCTAGGCGGCGCCGAAATCAGCATTAGCTGGGACGATGCCTTA
ACGCCTAAAGCCGAAACTGACGCCGAGTAA

Upstream 100 bases:

>100_bases
GTTTAGATGAGCTGAATGTACCGGGACTGGCCATAACGACTATTCATGGGAAAGGTTATTTATTCAATTACTTGCCAACC
CAAGGTCGGACCTAATCCAA

Downstream 100 bases:

>100_bases
TTGAGTGCTGAGGTTTAGTCTTTGACTAATAAGGCTAACACTTCGAAATGATCAGAGTGAGGAAAAAGATCGAATAACTG
CACCTTAGTTAACCGATAGC

Product: ATPase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKFQFYRLFIFLLLSCGIVIWSFGQLAEHFAHDEYNYQIDVDDLLQNYDEQLPKIKRIPVASLSLPENLQSSLQLGATIA
LRHSSNELYYYRLDKDSSHILVLGPVQTPVPKERNTSLILLGLYSSLCLVALWWIWPVFRDLNHLQQAAIEFGQAPRKRP
QDIHKHSAIFPLAKAFNSVSHQIVDFIQMHKELSRTISHEVRTPLARMRFALEIIRPQIDANYANRLNEDIDDIEQLAAN
YLSFARLEHKEETLSQESQSIELFMEKLAHKYAIYQPKFNIVFHYEAQQAHFDPIAMTIAIQNLVQNAMRFAQHDIHVHF
HQQRGINRISVEDDGPGFEGKGKKLVAAFERDSQQSDTSGYGLGLYIVKKIATWHYGTLELSRSQALGGAEISISWDDAL
TPKAETDAE

Sequences:

>Translated_409_residues
MKFQFYRLFIFLLLSCGIVIWSFGQLAEHFAHDEYNYQIDVDDLLQNYDEQLPKIKRIPVASLSLPENLQSSLQLGATIA
LRHSSNELYYYRLDKDSSHILVLGPVQTPVPKERNTSLILLGLYSSLCLVALWWIWPVFRDLNHLQQAAIEFGQAPRKRP
QDIHKHSAIFPLAKAFNSVSHQIVDFIQMHKELSRTISHEVRTPLARMRFALEIIRPQIDANYANRLNEDIDDIEQLAAN
YLSFARLEHKEETLSQESQSIELFMEKLAHKYAIYQPKFNIVFHYEAQQAHFDPIAMTIAIQNLVQNAMRFAQHDIHVHF
HQQRGINRISVEDDGPGFEGKGKKLVAAFERDSQQSDTSGYGLGLYIVKKIATWHYGTLELSRSQALGGAEISISWDDAL
TPKAETDAE
>Mature_409_residues
MKFQFYRLFIFLLLSCGIVIWSFGQLAEHFAHDEYNYQIDVDDLLQNYDEQLPKIKRIPVASLSLPENLQSSLQLGATIA
LRHSSNELYYYRLDKDSSHILVLGPVQTPVPKERNTSLILLGLYSSLCLVALWWIWPVFRDLNHLQQAAIEFGQAPRKRP
QDIHKHSAIFPLAKAFNSVSHQIVDFIQMHKELSRTISHEVRTPLARMRFALEIIRPQIDANYANRLNEDIDDIEQLAAN
YLSFARLEHKEETLSQESQSIELFMEKLAHKYAIYQPKFNIVFHYEAQQAHFDPIAMTIAIQNLVQNAMRFAQHDIHVHF
HQQRGINRISVEDDGPGFEGKGKKLVAAFERDSQQSDTSGYGLGLYIVKKIATWHYGTLELSRSQALGGAEISISWDDAL
TPKAETDAE

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=308, Percent_Identity=30.1948051948052, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789808, Length=254, Percent_Identity=27.1653543307087, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790346, Length=258, Percent_Identity=25.1937984496124, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790551, Length=296, Percent_Identity=26.0135135135135, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1788279, Length=216, Percent_Identity=25.9259259259259, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786912, Length=231, Percent_Identity=24.6753246753247, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47002; Mature: 47002

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFQFYRLFIFLLLSCGIVIWSFGQLAEHFAHDEYNYQIDVDDLLQNYDEQLPKIKRIPV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHCCHHHCCCC
ASLSLPENLQSSLQLGATIALRHSSNELYYYRLDKDSSHILVLGPVQTPVPKERNTSLIL
CCCCCCHHHHHHHHHCCEEEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCEEE
LGLYSSLCLVALWWIWPVFRDLNHLQQAAIEFGQAPRKRPQDIHKHSAIFPLAKAFNSVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
HQIVDFIQMHKELSRTISHEVRTPLARMRFALEIIRPQIDANYANRLNEDIDDIEQLAAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHH
YLSFARLEHKEETLSQESQSIELFMEKLAHKYAIYQPKFNIVFHYEAQQAHFDPIAMTIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEEECCCCCCCHHHHHHH
IQNLVQNAMRFAQHDIHVHFHQQRGINRISVEDDGPGFEGKGKKLVAAFERDSQQSDTSG
HHHHHHHHHHHHHHHHEEEEEHHCCCCEEEECCCCCCCCCCCCEEEEEHHCCCCCCCCCC
YGLGLYIVKKIATWHYGTLELSRSQALGGAEISISWDDALTPKAETDAE
CCHHHHHHHHHHHHCCCEEEECHHHCCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKFQFYRLFIFLLLSCGIVIWSFGQLAEHFAHDEYNYQIDVDDLLQNYDEQLPKIKRIPV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHCCHHHCCCC
ASLSLPENLQSSLQLGATIALRHSSNELYYYRLDKDSSHILVLGPVQTPVPKERNTSLIL
CCCCCCHHHHHHHHHCCEEEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCEEE
LGLYSSLCLVALWWIWPVFRDLNHLQQAAIEFGQAPRKRPQDIHKHSAIFPLAKAFNSVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
HQIVDFIQMHKELSRTISHEVRTPLARMRFALEIIRPQIDANYANRLNEDIDDIEQLAAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHH
YLSFARLEHKEETLSQESQSIELFMEKLAHKYAIYQPKFNIVFHYEAQQAHFDPIAMTIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEEECCCCCCCHHHHHHH
IQNLVQNAMRFAQHDIHVHFHQQRGINRISVEDDGPGFEGKGKKLVAAFERDSQQSDTSG
HHHHHHHHHHHHHHHHEEEEEHHCCCCEEEECCCCCCCCCCCCEEEEEHHCCCCCCCCCC
YGLGLYIVKKIATWHYGTLELSRSQALGGAEISISWDDALTPKAETDAE
CCHHHHHHHHHHHHCCCEEEECHHHCCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]