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The map label for this gene is prfB [H]

Identifier: 117921735

GI number: 117921735

Start: 3946347

End: 3947405

Strand: Reverse

Name: prfB [H]

Synonym: Shewana3_3297

Alternate gene names: 117921735

Gene position: 3947405-3946347 (Counterclockwise)

Preceding gene: 117921737

Following gene: 117921734

Centisome position: 79.39

GC content: 50.8

Gene sequence:

>1059_bases
TTGCCGAGCGTACGCAGCTTCTTAGGGGGTATCTTTGACTACGATGCTAAGCATGAGCGTCTAGAAGAAGTCAGCCGTGA
ACTTGAAAGTTCTGAGGTGTGGAACGAGCCAGAACGTGCTCAAGCCCTAGGTAAAGAGCGCGCCAGCCTCGAAGCCGTTG
TCAAAACTATCGATGATATGGACGCAGGTCTTGAAGACGTTGAAGGTCTGCTAGAACTTGCCATCGAAGAAGAAGACGAA
GACACCTTCAATGAAACCACCAAAGAGCTGGATTATTTAGAGAGCCGTTTGGCCGAACTCGAATTCCGCCGTATGTTCTC
TGGTCAACATGATGCCTCCGATTGTTACTTAGACATTCAATCGGGCAGTGGCGGTACTGAGGCACAGGACTGGGCCAACA
TGGTACTGCGTATGTACCTGCGTTGGGGCGAAGCCCACGGATTCAGCCCTGAGCTGATGGAAGTCACCGACGGTGACGTC
GCCGGCATTAAGGGCGCGACCATTAAATTCACCGGTGAGTATGCTTTCGGTTGGTTACGTACCGAGACGGGCGTGCACCG
TTTAGTGCGTAAATCGCCATTCGACTCATCGGGCCGTCGCCATACCTCTTTCTGCTCGGTATTCGTTTACCCAGAGATTG
ATGACGATATCACCATTGACATCAATCCTGCTGACTTACGTATCGACGTATACCGCGCCTCGGGTGCGGGTGGTCAGCAC
GTTAACCGAACCGAATCTGCGGTACGTATTACCCACTTACCGACTAACACGGTAGTGCAGTGCCAGAACGACCGTTCACA
GCACAAGAACAAAGATTCGGCGATGAAGCAGTTAAAAGCCAAACTGTTCGAATTAGAAATGCATAAGCAAAATGCGGAAA
AGCAAGCGGCCGAAGATGCTAAATCTGACATCGGTTGGGGCAGCCAGATCCGTTCTTATGTATTGGATGATTCCCGTATT
AAAGACTTACGCACTAGCGTGGAGACGCGCAATACCCAAGCGGTACTCGATGGCGATCTGGACAAGTTTATCGAAGCCAG
TTTGAAATCAGGGCTGTAA

Upstream 100 bases:

>100_bases
GCCGCCTCCTTTGATGGAAAATCCATTGAAACGGCAAAAATAAAGCGAAGTTGAAAATCCATGTTTGAAGTTAATCCAGT
AAAATTCAAAATTAAGGAGC

Downstream 100 bases:

>100_bases
TTAATTCAAAGGTATAGACGAGAAATAAGATGACTGAACAAGTACAAGATGAAAACAAACTGATTGCCGAGCGTCGTGCC
AAGCTAGAAAGCATTCGCCC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MPSVRSFLGGIFDYDAKHERLEEVSRELESSEVWNEPERAQALGKERASLEAVVKTIDDMDAGLEDVEGLLELAIEEEDE
DTFNETTKELDYLESRLAELEFRRMFSGQHDASDCYLDIQSGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDV
AGIKGATIKFTGEYAFGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPADLRIDVYRASGAGGQH
VNRTESAVRITHLPTNTVVQCQNDRSQHKNKDSAMKQLKAKLFELEMHKQNAEKQAAEDAKSDIGWGSQIRSYVLDDSRI
KDLRTSVETRNTQAVLDGDLDKFIEASLKSGL

Sequences:

>Translated_352_residues
MPSVRSFLGGIFDYDAKHERLEEVSRELESSEVWNEPERAQALGKERASLEAVVKTIDDMDAGLEDVEGLLELAIEEEDE
DTFNETTKELDYLESRLAELEFRRMFSGQHDASDCYLDIQSGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDV
AGIKGATIKFTGEYAFGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPADLRIDVYRASGAGGQH
VNRTESAVRITHLPTNTVVQCQNDRSQHKNKDSAMKQLKAKLFELEMHKQNAEKQAAEDAKSDIGWGSQIRSYVLDDSRI
KDLRTSVETRNTQAVLDGDLDKFIEASLKSGL
>Mature_351_residues
PSVRSFLGGIFDYDAKHERLEEVSRELESSEVWNEPERAQALGKERASLEAVVKTIDDMDAGLEDVEGLLELAIEEEDED
TFNETTKELDYLESRLAELEFRRMFSGQHDASDCYLDIQSGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDVA
GIKGATIKFTGEYAFGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPADLRIDVYRASGAGGQHV
NRTESAVRITHLPTNTVVQCQNDRSQHKNKDSAMKQLKAKLFELEMHKQNAEKQAAEDAKSDIGWGSQIRSYVLDDSRIK
DLRTSVETRNTQAVLDGDLDKFIEASLKSGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=307, Percent_Identity=33.2247557003257, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI34577120, Length=365, Percent_Identity=30.4109589041096, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI166795305, Length=214, Percent_Identity=34.5794392523364, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI2367172, Length=345, Percent_Identity=78.5507246376812, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1787462, Length=344, Percent_Identity=37.7906976744186, Blast_Score=202, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=242, Percent_Identity=31.404958677686, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=319, Percent_Identity=31.6614420062696, Blast_Score=139, Evalue=6e-34,
Organism=Drosophila melanogaster, GI19921226, Length=281, Percent_Identity=37.0106761565836, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39626; Mature: 39495

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSVRSFLGGIFDYDAKHERLEEVSRELESSEVWNEPERAQALGKERASLEAVVKTIDDM
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
DAGLEDVEGLLELAIEEEDEDTFNETTKELDYLESRLAELEFRRMFSGQHDASDCYLDIQ
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
SGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDVAGIKGATIKFTGEYAFGWLR
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEEECCEEEEEEE
TETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPADLRIDVYRASGAGGQH
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCEEEEEEEEECCCCCCC
VNRTESAVRITHLPTNTVVQCQNDRSQHKNKDSAMKQLKAKLFELEMHKQNAEKQAAEDA
CCCCCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSDIGWGSQIRSYVLDDSRIKDLRTSVETRNTQAVLDGDLDKFIEASLKSGL
HHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PSVRSFLGGIFDYDAKHERLEEVSRELESSEVWNEPERAQALGKERASLEAVVKTIDDM
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
DAGLEDVEGLLELAIEEEDEDTFNETTKELDYLESRLAELEFRRMFSGQHDASDCYLDIQ
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
SGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDVAGIKGATIKFTGEYAFGWLR
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEEECCEEEEEEE
TETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPADLRIDVYRASGAGGQH
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCEEEEEEEEECCCCCCC
VNRTESAVRITHLPTNTVVQCQNDRSQHKNKDSAMKQLKAKLFELEMHKQNAEKQAAEDA
CCCCCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSDIGWGSQIRSYVLDDSRIKDLRTSVETRNTQAVLDGDLDKFIEASLKSGL
HHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA