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The map label for this gene is glnG [H]

Identifier: 117921695

GI number: 117921695

Start: 3898399

End: 3899835

Strand: Reverse

Name: glnG [H]

Synonym: Shewana3_3257

Alternate gene names: 117921695

Gene position: 3899835-3898399 (Counterclockwise)

Preceding gene: 117921696

Following gene: 117921694

Centisome position: 78.43

GC content: 52.26

Gene sequence:

>1437_bases
ATGGACACTATTCTGATTGTCGACGATAACCATGCCATCTGCCAGGCCTTAGGTTTAATGCTGGAGATCAATGGTTATCA
GGTGTTGACTTGCCATACGCCCGAAGATGCCCTTAGCCTAATGGCAACCCAAGATGTTGATTTGGTTATCCAAGATATGA
ACTTCACCCGCGATACCACCTCGGGGGAAGAAGGGCGGCAGTTGTTTTATGCCCTGCGTGAGCGTCAGCAAGATCTGCCG
ATCATCCTGATGACGGCGTGGACTCAGCTTGAAACGGCGGTCGAGCTAGTGAAAGCGGGCGCCGCCGATTATATGGGTAA
ACCGTGGGATGATGCTAAGTTACTAAACAGTATCAATAATTTAATCGCGCTACATAAACTTGCCCGTGCAAACCAACAAC
TCGAGCGAGTGAACTCTCAGCGTCAGGTGGCGATCGCCGATGCGGATCTCTGTGGAATCGTGTTTGGCAGTGGCGCTATG
CAGCGCTGTATCGACTTAGCCTTACAACTGGCGCGCTCCGATGTGTCAGTGTTGATCACCGGCCCCAATGGTGCGGGTAA
AGACAAGCTTGCCGATATTATTCACGCCAATTCCCCCTTAAAAAACAAACCTTTTATCAAGGTGAATGTGGGCGCCTTGC
CGATGGAGTTGCTCGAAGCAGAACTCTTTGGTGCCGAGGCGGGAGCTTTTACCGGCGCCAATAAGGCGCGCATTGGTCGC
TTTGAGGCGGCCGATGGCGGCACGCTGTTTTTGGATGAAATTGGTAATTTACCCCTGTCGGGCCAAGTGAAATTGCTGCG
GGTATTACAAACCGGGGAGTTTGAGCGCTTAGGCAGCCATAAAACCTTAAAGGTGAAGGTGAGGGTGATCAGTGCGACCA
ACGCCGACCTGGCCCAAGATATTGCCGAGGGGCGTTTCCGTGAGGACTTATTCTACCGCTTGAATGTGATTGAATTGGCC
TTACCGCCGCTGAATCAACGAATTGACGATATCTTGCCCTTAGTGAAGCATTTTGTCGGCGAGGGTTTTAGCCTGAGTAA
ACCGGCGCAGCAAGCCCTGTTGCAGCACCGCTGGCCGGGCAATGTGCGGGAGCTGGAAAATGCCTGCAAACGTGCGGCGC
TCTTGGCCCAAAGCCGAGTGTTAACCGAAGCCGATTTTGGGTTACCACCCGCCCATGGAAGTCATTCCAGTGCAATAAAC
ACTACGACATTTGGGCGAACCCTGTCCGCAATAACACCGATTGAACCGCAGGTGAAAAGTTTGCATCATGACGTGCCTGC
TCAATCGGACTCCAGCGACGCTATGGATGTTAGCCGTGAGCAGATTGAAATGGCCCTAAGGCAACACAAGGGCGTGATTG
CGCGCGTGGCGAAATCCTTAGGCTTGAGTCGACAGGCCTTGTATCGCCGCATGGATAAGTTTGGTCTAGAAAAATAA

Upstream 100 bases:

>100_bases
TTAAATAAGTTGAAGGGCATTAAGTGCGATAACAGTAGGATGTTATTGCACTTTTTGTTATTTTAAGGTTAATAGTTTTA
CTCTGGACGCACATGATCAC

Downstream 100 bases:

>100_bases
CCATAGCATGATAAACACTGAGCATTAGGCGGAATTTTAGGTGCAACTGCGAACAAAATTAATCATTTATTCAACCGTTT
GTTGCGCCTTAGGTGTGCTC

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MDTILIVDDNHAICQALGLMLEINGYQVLTCHTPEDALSLMATQDVDLVIQDMNFTRDTTSGEEGRQLFYALRERQQDLP
IILMTAWTQLETAVELVKAGAADYMGKPWDDAKLLNSINNLIALHKLARANQQLERVNSQRQVAIADADLCGIVFGSGAM
QRCIDLALQLARSDVSVLITGPNGAGKDKLADIIHANSPLKNKPFIKVNVGALPMELLEAELFGAEAGAFTGANKARIGR
FEAADGGTLFLDEIGNLPLSGQVKLLRVLQTGEFERLGSHKTLKVKVRVISATNADLAQDIAEGRFREDLFYRLNVIELA
LPPLNQRIDDILPLVKHFVGEGFSLSKPAQQALLQHRWPGNVRELENACKRAALLAQSRVLTEADFGLPPAHGSHSSAIN
TTTFGRTLSAITPIEPQVKSLHHDVPAQSDSSDAMDVSREQIEMALRQHKGVIARVAKSLGLSRQALYRRMDKFGLEK

Sequences:

>Translated_478_residues
MDTILIVDDNHAICQALGLMLEINGYQVLTCHTPEDALSLMATQDVDLVIQDMNFTRDTTSGEEGRQLFYALRERQQDLP
IILMTAWTQLETAVELVKAGAADYMGKPWDDAKLLNSINNLIALHKLARANQQLERVNSQRQVAIADADLCGIVFGSGAM
QRCIDLALQLARSDVSVLITGPNGAGKDKLADIIHANSPLKNKPFIKVNVGALPMELLEAELFGAEAGAFTGANKARIGR
FEAADGGTLFLDEIGNLPLSGQVKLLRVLQTGEFERLGSHKTLKVKVRVISATNADLAQDIAEGRFREDLFYRLNVIELA
LPPLNQRIDDILPLVKHFVGEGFSLSKPAQQALLQHRWPGNVRELENACKRAALLAQSRVLTEADFGLPPAHGSHSSAIN
TTTFGRTLSAITPIEPQVKSLHHDVPAQSDSSDAMDVSREQIEMALRQHKGVIARVAKSLGLSRQALYRRMDKFGLEK
>Mature_478_residues
MDTILIVDDNHAICQALGLMLEINGYQVLTCHTPEDALSLMATQDVDLVIQDMNFTRDTTSGEEGRQLFYALRERQQDLP
IILMTAWTQLETAVELVKAGAADYMGKPWDDAKLLNSINNLIALHKLARANQQLERVNSQRQVAIADADLCGIVFGSGAM
QRCIDLALQLARSDVSVLITGPNGAGKDKLADIIHANSPLKNKPFIKVNVGALPMELLEAELFGAEAGAFTGANKARIGR
FEAADGGTLFLDEIGNLPLSGQVKLLRVLQTGEFERLGSHKTLKVKVRVISATNADLAQDIAEGRFREDLFYRLNVIELA
LPPLNQRIDDILPLVKHFVGEGFSLSKPAQQALLQHRWPGNVRELENACKRAALLAQSRVLTEADFGLPPAHGSHSSAIN
TTTFGRTLSAITPIEPQVKSLHHDVPAQSDSSDAMDVSREQIEMALRQHKGVIARVAKSLGLSRQALYRRMDKFGLEK

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=486, Percent_Identity=36.0082304526749, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI1788550, Length=489, Percent_Identity=37.8323108384458, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1790437, Length=480, Percent_Identity=36.6666666666667, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1788905, Length=389, Percent_Identity=40.6169665809769, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI87082117, Length=372, Percent_Identity=39.247311827957, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789087, Length=376, Percent_Identity=38.8297872340425, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1786524, Length=240, Percent_Identity=47.0833333333333, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87082152, Length=348, Percent_Identity=38.5057471264368, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1789233, Length=327, Percent_Identity=39.1437308868502, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI87081872, Length=215, Percent_Identity=46.9767441860465, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1787583, Length=339, Percent_Identity=35.1032448377581, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1789828, Length=273, Percent_Identity=34.7985347985348, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87081858, Length=325, Percent_Identity=29.5384615384615, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52370; Mature: 52370

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTILIVDDNHAICQALGLMLEINGYQVLTCHTPEDALSLMATQDVDLVIQDMNFTRDTT
CCEEEEEECCHHHHHHHCCEEEECCEEEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCC
SGEEGRQLFYALRERQQDLPIILMTAWTQLETAVELVKAGAADYMGKPWDDAKLLNSINN
CCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHH
LIALHKLARANQQLERVNSQRQVAIADADLCGIVFGSGAMQRCIDLALQLARSDVSVLIT
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHEECCHHHHHHHHHHHHHHCCCCEEEEE
GPNGAGKDKLADIIHANSPLKNKPFIKVNVGALPMELLEAELFGAEAGAFTGANKARIGR
CCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHCCC
FEAADGGTLFLDEIGNLPLSGQVKLLRVLQTGEFERLGSHKTLKVKVRVISATNADLAQD
EECCCCCEEEHHHHCCCCCCCCHHHHHHHHCCCHHHHCCCCEEEEEEEEEECCCHHHHHH
IAEGRFREDLFYRLNVIELALPPLNQRIDDILPLVKHFVGEGFSLSKPAQQALLQHRWPG
HHHCHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCC
NVRELENACKRAALLAQSRVLTEADFGLPPAHGSHSSAINTTTFGRTLSAITPIEPQVKS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCHHHHCCCCCHHHHH
LHHDVPAQSDSSDAMDVSREQIEMALRQHKGVIARVAKSLGLSRQALYRRMDKFGLEK
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDTILIVDDNHAICQALGLMLEINGYQVLTCHTPEDALSLMATQDVDLVIQDMNFTRDTT
CCEEEEEECCHHHHHHHCCEEEECCEEEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCC
SGEEGRQLFYALRERQQDLPIILMTAWTQLETAVELVKAGAADYMGKPWDDAKLLNSINN
CCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHH
LIALHKLARANQQLERVNSQRQVAIADADLCGIVFGSGAMQRCIDLALQLARSDVSVLIT
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHEECCHHHHHHHHHHHHHHCCCCEEEEE
GPNGAGKDKLADIIHANSPLKNKPFIKVNVGALPMELLEAELFGAEAGAFTGANKARIGR
CCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHCCC
FEAADGGTLFLDEIGNLPLSGQVKLLRVLQTGEFERLGSHKTLKVKVRVISATNADLAQD
EECCCCCEEEHHHHCCCCCCCCHHHHHHHHCCCHHHHCCCCEEEEEEEEEECCCHHHHHH
IAEGRFREDLFYRLNVIELALPPLNQRIDDILPLVKHFVGEGFSLSKPAQQALLQHRWPG
HHHCHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCC
NVRELENACKRAALLAQSRVLTEADFGLPPAHGSHSSAINTTTFGRTLSAITPIEPQVKS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCHHHHCCCCCHHHHH
LHHDVPAQSDSSDAMDVSREQIEMALRQHKGVIARVAKSLGLSRQALYRRMDKFGLEK
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]