Click here to switch to the map view.

The map label for this gene is purT [H]

Identifier: 117921635

GI number: 117921635

Start: 3835696

End: 3836871

Strand: Direct

Name: purT [H]

Synonym: Shewana3_3197

Alternate gene names: 117921635

Gene position: 3835696-3836871 (Clockwise)

Preceding gene: 117921630

Following gene: 117921636

Centisome position: 77.14

GC content: 53.66

Gene sequence:

>1176_bases
ATGATAGGAACTCCCTACACAGAGGGCGCTCGACGCGCCATGTTGCTTGGCTGCGGTGAGCTAGGTAAAGAAGTCGCCAT
CGAGCTCCAACGCTTAGGTGTTGAAGTGATTGGCGTCGATCGTTATCCTAATGCCCCTGCCATGCAAATTGCCCATCGCT
CCCATGTGATCAATATGCTCGACGCTAAAGCGCTTCGCGCCATTATCGAGCTAGAAAAGCCCCACTTAGTGATCCCCGAA
ATTGAAGCCATCGCCACTCAGACCTTGGTCGAGATGGAAACCGAAGGCCTCAATGTCGTGCCGACCGCACGCGCGACTCA
GCTGACCATGGACAGAGAAGGTATTCGTCGCCTCGCAGCCGAAACCTTAGGTCTACCGACCTCCCCCTATTTCTTCTGCG
ACACTGAAACCGAGTTTAATCAAGCCATTGGCAAGATTGGCGTACCCTGCGTGGTCAAACCCGTGATGAGTTCATCGGGC
AAGGGCCAGAGTGTTATCCGTGATGCGGCCCAAAGCACCAAAGCTTGGCAATACGCCCAAGAAGGCGGCCGCGCGGGCGG
TGGCCGTGTGATTGTTGAAGGCTTTATCCCCTTCGATTACGAAATTACCCTGCTGACCATTAGTGCGGTTAACGGCATCC
ACTTCTGCGCGCCAATTGGCCACAGACAAGAAGACGGCGACTACCGCGAGTCATGGCAACCTCAAGCCATGTCGGCCGAC
GTGCTGGCAAAATCCCAAGCAATCGCCAGCAAAGTGGTCGAAGCCCTCGGCGGTTATGGCTTATTTGGGGTCGAGCTGTT
TGTGAAGGGGAGCGATGTGTACTTCTCTGAAGTCTCGCCTCGTCCGCACGATACCGGTTTAGTCACCTTAATTAGCCAAG
ATTTATCCGAGTTTGCACTGCACGTTAGGGCGATTCTTGGTTTGCCCATTCCGAATATCCACCAACATGGCCCCAGCGCC
TCGGCGGTAGTATTGGTGGAAGGCAAATCGAAAAACATTCGCTATCAAGGTCTTGCCGATGCATTGGCGGCGGAAAATAC
TCAGCTCAGATTATTCGCTAAACCTGAAATCGATGGTCGCCGCCGTTTAGGGGTGGCCCTCGCCCGCGATAAGGATATCG
AAAGCGCAGTCAACAAAGCGTTGGATAGTGCATCTAAGGTAAAAGTGATTTTCTAG

Upstream 100 bases:

>100_bases
TGTTAATTTCAGTCTCATGCTTTAGCGCCAATCGGGGTAGACTATGGCCCAAGAACGTCTAAAGGCGTCAATTATCCCAA
TATAAAAAGGTACCCACACC

Downstream 100 bases:

>100_bases
GCATGAATGCTTAACGACTTGTCGCCAATACCTTAATGTTTAGTACTGTGATCAGCACCGCGCCCAGCACTATTGCTGGG
CGCGTTATTAAGGCGTTTAA

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MIGTPYTEGARRAMLLGCGELGKEVAIELQRLGVEVIGVDRYPNAPAMQIAHRSHVINMLDAKALRAIIELEKPHLVIPE
IEAIATQTLVEMETEGLNVVPTARATQLTMDREGIRRLAAETLGLPTSPYFFCDTETEFNQAIGKIGVPCVVKPVMSSSG
KGQSVIRDAAQSTKAWQYAQEGGRAGGGRVIVEGFIPFDYEITLLTISAVNGIHFCAPIGHRQEDGDYRESWQPQAMSAD
VLAKSQAIASKVVEALGGYGLFGVELFVKGSDVYFSEVSPRPHDTGLVTLISQDLSEFALHVRAILGLPIPNIHQHGPSA
SAVVLVEGKSKNIRYQGLADALAAENTQLRLFAKPEIDGRRRLGVALARDKDIESAVNKALDSASKVKVIF

Sequences:

>Translated_391_residues
MIGTPYTEGARRAMLLGCGELGKEVAIELQRLGVEVIGVDRYPNAPAMQIAHRSHVINMLDAKALRAIIELEKPHLVIPE
IEAIATQTLVEMETEGLNVVPTARATQLTMDREGIRRLAAETLGLPTSPYFFCDTETEFNQAIGKIGVPCVVKPVMSSSG
KGQSVIRDAAQSTKAWQYAQEGGRAGGGRVIVEGFIPFDYEITLLTISAVNGIHFCAPIGHRQEDGDYRESWQPQAMSAD
VLAKSQAIASKVVEALGGYGLFGVELFVKGSDVYFSEVSPRPHDTGLVTLISQDLSEFALHVRAILGLPIPNIHQHGPSA
SAVVLVEGKSKNIRYQGLADALAAENTQLRLFAKPEIDGRRRLGVALARDKDIESAVNKALDSASKVKVIF
>Mature_391_residues
MIGTPYTEGARRAMLLGCGELGKEVAIELQRLGVEVIGVDRYPNAPAMQIAHRSHVINMLDAKALRAIIELEKPHLVIPE
IEAIATQTLVEMETEGLNVVPTARATQLTMDREGIRRLAAETLGLPTSPYFFCDTETEFNQAIGKIGVPCVVKPVMSSSG
KGQSVIRDAAQSTKAWQYAQEGGRAGGGRVIVEGFIPFDYEITLLTISAVNGIHFCAPIGHRQEDGDYRESWQPQAMSAD
VLAKSQAIASKVVEALGGYGLFGVELFVKGSDVYFSEVSPRPHDTGLVTLISQDLSEFALHVRAILGLPIPNIHQHGPSA
SAVVLVEGKSKNIRYQGLADALAAENTQLRLFAKPEIDGRRRLGVALARDKDIESAVNKALDSASKVKVIF

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=389, Percent_Identity=67.866323907455, Blast_Score=546, Evalue=1e-157,
Organism=Escherichia coli, GI1786733, Length=255, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324702, Length=373, Percent_Identity=24.6648793565684, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42234; Mature: 42234

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGTPYTEGARRAMLLGCGELGKEVAIELQRLGVEVIGVDRYPNAPAMQIAHRSHVINML
CCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHH
DAKALRAIIELEKPHLVIPEIEAIATQTLVEMETEGLNVVPTARATQLTMDREGIRRLAA
HHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECHHHHHHHHH
ETLGLPTSPYFFCDTETEFNQAIGKIGVPCVVKPVMSSSGKGQSVIRDAAQSTKAWQYAQ
HHHCCCCCCEEEECCCCHHHHHHHHCCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EGGRAGGGRVIVEGFIPFDYEITLLTISAVNGIHFCAPIGHRQEDGDYRESWQPQAMSAD
HCCCCCCCEEEEEEECCCCEEEEEEEEEECCCEEEECCCCCCCCCCCHHHCCCCCCCCHH
VLAKSQAIASKVVEALGGYGLFGVELFVKGSDVYFSEVSPRPHDTGLVTLISQDLSEFAL
HHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHH
HVRAILGLPIPNIHQHGPSASAVVLVEGKSKNIRYQGLADALAAENTQLRLFAKPEIDGR
HHHHHHCCCCCCHHCCCCCCCEEEEEECCCCCEEECCHHHHHHCCCCEEEEEECCCCCCC
RRLGVALARDKDIESAVNKALDSASKVKVIF
HHHCEEEECCCCHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MIGTPYTEGARRAMLLGCGELGKEVAIELQRLGVEVIGVDRYPNAPAMQIAHRSHVINML
CCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHH
DAKALRAIIELEKPHLVIPEIEAIATQTLVEMETEGLNVVPTARATQLTMDREGIRRLAA
HHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECHHHHHHHHH
ETLGLPTSPYFFCDTETEFNQAIGKIGVPCVVKPVMSSSGKGQSVIRDAAQSTKAWQYAQ
HHHCCCCCCEEEECCCCHHHHHHHHCCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EGGRAGGGRVIVEGFIPFDYEITLLTISAVNGIHFCAPIGHRQEDGDYRESWQPQAMSAD
HCCCCCCCEEEEEEECCCCEEEEEEEEEECCCEEEECCCCCCCCCCCHHHCCCCCCCCHH
VLAKSQAIASKVVEALGGYGLFGVELFVKGSDVYFSEVSPRPHDTGLVTLISQDLSEFAL
HHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHH
HVRAILGLPIPNIHQHGPSASAVVLVEGKSKNIRYQGLADALAAENTQLRLFAKPEIDGR
HHHHHHCCCCCCHHCCCCCCCEEEEEECCCCCEEECCHHHHHHCCCCEEEEEECCCCCCC
RRLGVALARDKDIESAVNKALDSASKVKVIF
HHHCEEEECCCCHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA